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rabbit anti esrrb polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech rabbit anti esrrb polyclonal antibody

    Rabbit Anti Esrrb Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal/ESRRB+Antibody/pmc13657169-14-0-5
    Average 93 stars, based on 9 article reviews
    rabbit anti esrrb polyclonal antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells"

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells

    Journal: iScience

    doi: 10.1016/j.isci.2026.117658


    Figure Legend Snippet:

    Techniques Used: Cell-Signaling, Magnetic Beads, Virus, Recombinant, ROS Assay, Reverse Transcription, SYBR Green Assay, Staining, Plasmid Preparation, Flow Cytometry, Western Blot, Expressing, Control, Software

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    Article Title: Resveratrol and AG490 Overcome Glioblastoma Cells' Resistance to Monotherapy by Inhibiting JAK2/STAT3 Signalling Pathway.
    Article Snippet: The cells were lysed using RIPA buffer supplemented with protease and phosphatase inhibitors for 30 min. A BCA kit (Beyotime Biotechnology, Shanghai, China) was used to measure protein concentration, and then separated by 10% SDS-PAGE (G217750T Servicebio), and transferred to PVDF membranes. .. Afterword, the membranes were blocked with 5% skim milk for 2 h, and washed thrice with Tris-buffered saline (TBS-T, 8 min each), and incubated overnight at 4 ◦C with primary antibodies, Rabbit polyclonal anti STAT3 (1:1000, Protein Tech, Rosemont, IL, USA 10253-2-AP), Rabbit polyclonal anti pSTAT3 (1:1000, abs118973), Rabbit polyclonal anti BAX (1:1000, Protein Tech, USA 50599- 2-lg), Rabbit polyclonal anti BCL-2 (1:1000, Protein Tech, USA 26593-1-AP), and Rabbit polyclonal anti- GAPDH (1:5000, Proteintech, Wuhan, China 10494-1-AP). .. The membranes were then washed three times with TBS-T for 8 min and incubated with HRP-conjugated goat anti-rabbit (1:3000, SE134, Solarbio, Beijing, China) secondary antibody for 2 h at 4 ◦C.

    Incubation:

    Article Title: Resveratrol and AG490 Overcome Glioblastoma Cells' Resistance to Monotherapy by Inhibiting JAK2/STAT3 Signalling Pathway.
    Article Snippet: The cells were lysed using RIPA buffer supplemented with protease and phosphatase inhibitors for 30 min. A BCA kit (Beyotime Biotechnology, Shanghai, China) was used to measure protein concentration, and then separated by 10% SDS-PAGE (G217750T Servicebio), and transferred to PVDF membranes. .. Afterword, the membranes were blocked with 5% skim milk for 2 h, and washed thrice with Tris-buffered saline (TBS-T, 8 min each), and incubated overnight at 4 ◦C with primary antibodies, Rabbit polyclonal anti STAT3 (1:1000, Protein Tech, Rosemont, IL, USA 10253-2-AP), Rabbit polyclonal anti pSTAT3 (1:1000, abs118973), Rabbit polyclonal anti BAX (1:1000, Protein Tech, USA 50599- 2-lg), Rabbit polyclonal anti BCL-2 (1:1000, Protein Tech, USA 26593-1-AP), and Rabbit polyclonal anti- GAPDH (1:5000, Proteintech, Wuhan, China 10494-1-AP). .. The membranes were then washed three times with TBS-T for 8 min and incubated with HRP-conjugated goat anti-rabbit (1:3000, SE134, Solarbio, Beijing, China) secondary antibody for 2 h at 4 ◦C.

    Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration
    Article Snippet: Equal protein aliquots were resolved on 10 % SDS-PAGE gels (Bio-Rad, #4561033) at 100 V for 2 h and transferred to PVDF membranes (Millipore, #IPFL00010) via electrophoresis (300 mA, 30 min). .. Membranes were blocked with 5 % non-fat milk (BD DifcoTM, #232100) in TBST (Beyotime, #ST673) for 1 h at 25 °C and incubated overnight at 4 °C with the following primary antibodies: Osteopontin (OPN) : • Rabbit monoclonal (Proteintech, 22952-1-AP), 1:1000 • Collagen I (COL1A1): Rabbit polyclonal (Proteintech, 14695-1-AP), 1:1000 • RUNX2: Rabbit monoclonal (Proteintech, 20700-1-AP), 1:1000 • NRF2: Rabbit monoclonal (Proteintech, 16396-1-AP), 1:2000 • NQO1: Mouse monoclonal (Proteintech, 67240-1-Ig), 1:2000 • Beta Actin: Mouse monoclonal (Proteintech, 66009-1-Ig), 1:20,000 After three 10-min TBST washes, membranes were incubated with HRP-conjugated goat anti-rabbit/mouse IgG (Beyotime, #A0208/#A0216, 1:5000) for 2 h at 25 °C. .. Protein bands were visualized using ECL Prime (Beyotime, #P0018) and imaged on a ChemiDoc MP System (Bio-Rad).

    Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration
    Article Snippet: .. Cells were fixed with 4 % paraformaldehyde (Servicebio, #G1101) for 15 min at 25 °C, permeabilized with 0.3 % Triton X-100 in PBS for 15 min, and blocked with 5 % BSA (Sigma, #A7906) containing 10 % normal goat serum (Servicebio, #G5009) for 1 h. Primary antibodies were diluted in antibody diluent (Servicebio, #G1212) and incubated overnight at 4 °C: • Osteopontin (OPN): Rabbit monoclonal (Proteintech, #22952-1-AP), 1:500 • Osteocalcin (OCN): Rabbit polyclonal (Proteintech, #20277-1-AP), 1:500 • iNOS: Mouse anti-iNOS (Proteintech, #22226-1-AP), 1:500 • CD206: Rabbit anti-CD206 (Proteintech, #18704-1-AP), 1:500 After three PBS washes, species-matched secondary antibodies were applied for 1 h at 25 °C in the dark: • Alexa Fluor 488: Goat anti-rabbit IgG (Servicebio, #GB25303), 1:500 • Alexa Fluor 488: Goat anti-mouse IgG (Servicebio, #GB25301), 1:500 • Cy3: Goat anti-rabbit IgG (Servicebio, # GB21303), 1:500 Nuclei were counterstained with DAPI (Servicebio, #G1407). .. Total RNA was isolated from OIM-induced ADSCs at day 7 using TRIzolTM Reagent (Invitrogen, #15596–026) per manufacturer's protocol.

    Article Title: No Correlation Between Interferon Signaling and Cytosolic Mitochondrial DNA/RNA Leakage in Cultured Skin Fibroblasts of Patients With Mitochondrial Diseases
    Article Snippet: The fold change was then calculated with the formula 2 −ΔΔC t . The qPCR was done on the Viia7 Real Time machine (Thermo Fisher Scientific), and at least >6 determinations on successive passages were performed. .. Fibroblasts were grown in an Opera 96‐well plate, washed, and fixed with 4% paraformaldehyde (sc‐281692, ChemCruz) for 15 min. After three washes with PBS+1% BSA, cells were permeabilized with 0.1% Triton X‐100 in PBS+1% BSA for 10 min, washed, and incubated overnight at 4°C with primary antibody mouse monoclonal anti‐dsDNA (CBL186, Merck) or mouse monoclonal anti‐dsRNA J2 (10010200‐200UG, ThermoFischer) and rabbit polyclonal anti‐TOMM40 (18409‐1‐AP, Proteintech), 1:1000 dilution. ..

    other:

    Article Title: Disturbed mitochondrial maturation in cardiolipin remodeling-deficient cardiomyocytes
    Article Snippet: anti-TOM20, rabbit polyclonal , Proteintech , Cat#11802-1-AP; RRID: AB_2207530.

    Generated:

    Article Title: TRIM9 switches the morphological phenotype of melanoma cells
    Article Snippet: .. Antibodies used : rabbit polyclonal anti GFP (A11122, Invitrogen, RRID:AB_221569), rabbit polyclonal anti TRIM9 (generated in house), rabbit polyclonal anti TRIM9 (Proteintech 10786-1-AP), chicken polyclonal anti-GFP (GFP-1010, Aves), mouse monoclonal anti paxillin (BD Transduction Laboratories 610051), mouse monoclonal anti GAPDH WB(Santa Cruz mouse (D-6): sc-166545), rabbit polyclonal anti VASP (Sigma-Aldrich rabbit Anti-VASP (C-terminal) antibody: V3390), rabbit anti VASP (Cell Signaling Technology antirabbit Phospho-VASP (Ser157) Antibody #3111), rabbit monoclonal anti phospho-FAK (Tyr397 (Invitrogen 44-625G), mouse monoclonal anti FAK (Invitrogen 39-6500), Alexa Flour Phalloidin 488 (Fisher A12379), Alexa Flour Phalloidin 568 (A12380). ..

    Western Blot:

    Article Title: TRIM9 switches the morphological phenotype of melanoma cells
    Article Snippet: .. Antibodies used : rabbit polyclonal anti GFP (A11122, Invitrogen, RRID:AB_221569), rabbit polyclonal anti TRIM9 (generated in house), rabbit polyclonal anti TRIM9 (Proteintech 10786-1-AP), chicken polyclonal anti-GFP (GFP-1010, Aves), mouse monoclonal anti paxillin (BD Transduction Laboratories 610051), mouse monoclonal anti GAPDH WB(Santa Cruz mouse (D-6): sc-166545), rabbit polyclonal anti VASP (Sigma-Aldrich rabbit Anti-VASP (C-terminal) antibody: V3390), rabbit anti VASP (Cell Signaling Technology antirabbit Phospho-VASP (Ser157) Antibody #3111), rabbit monoclonal anti phospho-FAK (Tyr397 (Invitrogen 44-625G), mouse monoclonal anti FAK (Invitrogen 39-6500), Alexa Flour Phalloidin 488 (Fisher A12379), Alexa Flour Phalloidin 568 (A12380). ..



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    Image Search Results


    Journal: iScience

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells

    doi: 10.1016/j.isci.2026.117658

    Figure Lengend Snippet:

    Article Snippet: Rabbit anti-ESRRB polyclonal antibody , Proteintech , 22644-1-AP; RRID: AB_2879140.

    Techniques: Cell-Signaling, Magnetic Beads, Virus, Recombinant, ROS Assay, Reverse Transcription, SYBR Green Assay, Staining, Plasmid Preparation, Flow Cytometry, Western Blot, Expressing, Control, Software

    Deletion of Pex19 impairs the growth of mESCs (A) Pex19 deficiency reduces the growth of mESCs. Equal numbers of WT and Pex19 KO 46C mESCs were seeded and cultured in ESGRO medium, and cell counts were analyzed on day 3 ( n = 3 biological replicates/group; data are presented as mean ± SEM; Student’s t test, ∗∗ p < 0.01). (B) Pex19 deficiency reduces the single-cell self-renewal capacity of mESCs. WT and Pex19 KO mESCs were cultured for colony formation for 5 days ( n = 3 biological replicates/group; data are presented as mean ± SEM, Student’s t test, ∗ p < 0.05). (C) Pex19 deficiency increases cell death and apoptosis of mESCs ( n = 3 biological replicates/group, Student’s t test, ∗∗ p < 0.01, ∗∗∗ p < 0.001). (D) Adding back PEX19 restores the peroxisomal targeting of PEX14. Scale bars, 5 μm. (E–G) Adding back PEX19 rescues defective cell growth and elevated cell death in Pex19 KO mESCs. E and F, n = 3–4 biological replicates/group; data are presented as mean ± SEM, one-way ANOVA, ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001, n.s., not significant). (H) Top enriched GSEA pathways in Pex19 KO vs. WT mESCs. Transcriptomes of WT and Pex19 KO 46C mESCs from the RNA-seq dataset were analyzed using GSEA as described in . NES, Normalized Enrichment Score; FDR, False Discovery Rate. See also .

    Journal: iScience

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells

    doi: 10.1016/j.isci.2026.117658

    Figure Lengend Snippet: Deletion of Pex19 impairs the growth of mESCs (A) Pex19 deficiency reduces the growth of mESCs. Equal numbers of WT and Pex19 KO 46C mESCs were seeded and cultured in ESGRO medium, and cell counts were analyzed on day 3 ( n = 3 biological replicates/group; data are presented as mean ± SEM; Student’s t test, ∗∗ p < 0.01). (B) Pex19 deficiency reduces the single-cell self-renewal capacity of mESCs. WT and Pex19 KO mESCs were cultured for colony formation for 5 days ( n = 3 biological replicates/group; data are presented as mean ± SEM, Student’s t test, ∗ p < 0.05). (C) Pex19 deficiency increases cell death and apoptosis of mESCs ( n = 3 biological replicates/group, Student’s t test, ∗∗ p < 0.01, ∗∗∗ p < 0.001). (D) Adding back PEX19 restores the peroxisomal targeting of PEX14. Scale bars, 5 μm. (E–G) Adding back PEX19 rescues defective cell growth and elevated cell death in Pex19 KO mESCs. E and F, n = 3–4 biological replicates/group; data are presented as mean ± SEM, one-way ANOVA, ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001, n.s., not significant). (H) Top enriched GSEA pathways in Pex19 KO vs. WT mESCs. Transcriptomes of WT and Pex19 KO 46C mESCs from the RNA-seq dataset were analyzed using GSEA as described in . NES, Normalized Enrichment Score; FDR, False Discovery Rate. See also .

    Article Snippet: Rabbit anti-PEX14 polyclonal antibody , Proteintech , 10594-1-AP; RRID: AB_2252194.

    Techniques: Cell Culture, Single Cell, Colony Assay, RNA Sequencing

    Journal: iScience

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells

    doi: 10.1016/j.isci.2026.117658

    Figure Lengend Snippet:

    Article Snippet: Rabbit anti-PEX14 polyclonal antibody , Proteintech , 10594-1-AP; RRID: AB_2252194.

    Techniques: Cell-Signaling, Magnetic Beads, Virus, Recombinant, ROS Assay, Reverse Transcription, SYBR Green Assay, Staining, Plasmid Preparation, Flow Cytometry, Western Blot, Expressing, Control, Software

    Deletion of Pex19 induces mitotic block in mESCs (A) Deletion of Pex19 impairs cell cycle progression and induces polynuclear mESCs. WT, Pex19 KO, and Pex19 KO + PEX19 46C mESCs were stained with PI for DNA content, then subjected to FACS analysis ( n = 3 biological replicates/group; data are presented as mean ± SEM). (B) Deletion of Pex5 in mESCs fails to induce detectable defects in the cell cycle. WT, Pex5 KO, and Pex19 KO 46C mESCs were stained with DAPI for DNA content, then subjected to FACS analysis. (C) Deletion of Pex19 but not Pex5 induces a new fraction of polynuclear mitotic cells. WT, Pex5 KO, and Pex19 KO 46C mESCs treated with or without 50 ng/mL nocodazole were stained with an anti-H3pS10 antibody for detection of mitotic cells and DAPI for DNA content, then subjected to FACS analysis. The M-B population represents polynuclear mitotic cells arrested by deletion of Pex19 or treatment with nocodazole. (D and E) Deletion of Pex19 delays cell cycle progression of mESCs. WT and Pex19 KO 46C mESCs treated with 50 ng/mL nocodazole for 5 h were transferred into normal culture medium without nocodazole, and cell cycle progression was analyzed 15 and 25 min later (three groups of cells (80–250 cells/group) from randomly selected fields were analyzed for each time point for both WT and Pex19 KO mESCs; data are presented as mean ± SEM). Scale bars, 10 μm. See also .

    Journal: iScience

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells

    doi: 10.1016/j.isci.2026.117658

    Figure Lengend Snippet: Deletion of Pex19 induces mitotic block in mESCs (A) Deletion of Pex19 impairs cell cycle progression and induces polynuclear mESCs. WT, Pex19 KO, and Pex19 KO + PEX19 46C mESCs were stained with PI for DNA content, then subjected to FACS analysis ( n = 3 biological replicates/group; data are presented as mean ± SEM). (B) Deletion of Pex5 in mESCs fails to induce detectable defects in the cell cycle. WT, Pex5 KO, and Pex19 KO 46C mESCs were stained with DAPI for DNA content, then subjected to FACS analysis. (C) Deletion of Pex19 but not Pex5 induces a new fraction of polynuclear mitotic cells. WT, Pex5 KO, and Pex19 KO 46C mESCs treated with or without 50 ng/mL nocodazole were stained with an anti-H3pS10 antibody for detection of mitotic cells and DAPI for DNA content, then subjected to FACS analysis. The M-B population represents polynuclear mitotic cells arrested by deletion of Pex19 or treatment with nocodazole. (D and E) Deletion of Pex19 delays cell cycle progression of mESCs. WT and Pex19 KO 46C mESCs treated with 50 ng/mL nocodazole for 5 h were transferred into normal culture medium without nocodazole, and cell cycle progression was analyzed 15 and 25 min later (three groups of cells (80–250 cells/group) from randomly selected fields were analyzed for each time point for both WT and Pex19 KO mESCs; data are presented as mean ± SEM). Scale bars, 10 μm. See also .

    Article Snippet: Rabbit anti-PEX5 polyclonal antibody , Novus , NBP1-87185; RRID: AB_11023925.

    Techniques: Blocking Assay, Staining, Analysis

    Journal: iScience

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells

    doi: 10.1016/j.isci.2026.117658

    Figure Lengend Snippet:

    Article Snippet: Rabbit anti-PEX5 polyclonal antibody , Novus , NBP1-87185; RRID: AB_11023925.

    Techniques: Cell-Signaling, Magnetic Beads, Virus, Recombinant, ROS Assay, Reverse Transcription, SYBR Green Assay, Staining, Plasmid Preparation, Flow Cytometry, Western Blot, Expressing, Control, Software

    Journal: iScience

    Article Title: M2 type tumor-associated macrophage-derived CCL-18 promotes glioma cell proliferation but does not mediates glioma recurrence after radiotherapy

    doi: 10.1016/j.isci.2026.117695

    Figure Lengend Snippet:

    Article Snippet: Anti-p65 rabbit antibody , Proteintech , 10745-1-AP; RRID: AB_2178878.

    Techniques: Cell-Signaling, Virus, Tissue, Microarray, Recombinant, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Software, Biomarker Discovery, In Vivo Imaging