rabbit polyclonal tlr4 ab  (Boster Bio)


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    Name:
    Anti Toll like receptor 4 TLR4 Antibody
    Description:
    Polyclonal antibody for TLR4 detection Host Rabbit Size 100μg vial Tested applications IHC P Reactive species Human TLR4 information Molecular Weight 95680 MW Subcellular Localization Cell membrane Single pass type I membrane protein Upon complex formation with CD36 and TLR6 internalized through dynamin dependent endocytosis Tissue Specificity Highly expressed in placenta spleen and peripheral blood leukocytes Detected in monocytes macrophages dendritic cells and several types of T cells
    Catalog Number:
    pa1484
    Price:
    280.0
    Applications:
    IHC, WB
    Host:
    Rabbit
    Immunogen:
    A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4(50-68aa DNLPFSTKNLDLSFNPLRH).
    Category:
    Primary Antibodies
    Reactivity:
    Human
    Buy from Supplier


    Structured Review

    Boster Bio rabbit polyclonal tlr4 ab
    Anti Toll like receptor 4 TLR4 Antibody
    Polyclonal antibody for TLR4 detection Host Rabbit Size 100μg vial Tested applications IHC P Reactive species Human TLR4 information Molecular Weight 95680 MW Subcellular Localization Cell membrane Single pass type I membrane protein Upon complex formation with CD36 and TLR6 internalized through dynamin dependent endocytosis Tissue Specificity Highly expressed in placenta spleen and peripheral blood leukocytes Detected in monocytes macrophages dendritic cells and several types of T cells
    https://www.bioz.com/result/rabbit polyclonal tlr4 ab/product/Boster Bio
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal tlr4 ab - by Bioz Stars, 2021-01
    94/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: H19 promote calcium oxalate nephrocalcinosis-induced renal tubular epithelial cell injury via a ceRNA pathway
    Article Snippet: .. For IHC, sections were incubated overnight at 4 °C with anti-HMGB1, anti-TLR4, anti-NF-kB, anti-SOD2 (Boster, China, BM4813) and anti-NOX2 (Boster, China, BA2811) antibodies and then detected using an Envision HRP Polymer System (Boster, China). .. All images were acquired by a high-throughput Leica SCN400 scanner.

    BIA-KA:

    Article Title: Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor κB and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis
    Article Snippet: .. Materials TLR4 antibody and NF-κB antibody (Boster, Wuhan, China), NF-κB p65 antibody (Ebioscience, USA), BCA protein concentration Kit (Pierce, USA), reverse transcription polymerase chain reaction (RT-PCR) Kit (Gibco, USA), TRIzol (Invitrogen, USA), PCR primer and DNA ladder (Sbsgene, China). .. Animal grouping and ANP model development Seventy two male SD rats were divided into three groups with 24 rats each as sham-operation, ANP, and ANP with PDTC pretreatment.

    Blocking Assay:

    Article Title: Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens
    Article Snippet: .. The membranes were blocked with blocking buffer (Beyotime) at room temperature for 2 h and then were incubated with anti-phospho-P38 [#4511T, anti-rabbit, Cell Signaling Technology (CST), USA], anti-P38 (#9212S, anti-rabbit, CST), anti-phospho-JNK (#4668S, anti-rabbit, CST), anti-JNK (#928, anti-rabbit, CST), anti-phospho-ERK (#9101S, anti-rabbit, CST), anti-ERK (#9102S, anti-rabbit, CST), anti-TLR-3 (NBP2-24565, anti-rabbit, NBP2-24565Novus Biologicals, USA), and anti-TLR-4 (BA1717, anti-rabbit, Boster, China) primary antibodies overnight at 4°C, followed by incubation with the corresponding horseradish peroxidase-conjugated secondary antibody (Beyotime) at 4°C for 4 h. The protein–antibody complexes were detected with the ECL Plus A and B (Beyotime), and the results were quantified using the Fusion FX software (Vilber, France). .. Nuclear and cytoplasmic proteins were prepared using the nuclear and cytoplasmic protein extraction kit (Beyotime), and then the levels of NF-κB p65 (D14E12) subunit (#3033P, anti-rabbit, CST), nuclear factor erythroid 2 p45-related factor 2 (Nrf2) (ab62352, anti-rabbit; Abcam, Britain), and inhibitory protein-κBα (IκBα) (44D4) (#4812S, anti-rabbit, CST) were detected.

    Article Title: Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury
    Article Snippet: .. The membranes were washed in Tris-buffered saline containing 0.05% Tween-20 (TBST) followed by blocking for 1 h using 5% nonfat milk in TBST at room temperature, then incubated at 4°C overnight with the following primary antibodies: monoclonaln antibody to Prdx6 (1:1000; ab133348, Abcam, Cambridge, MA, USA), rabbit polyclonal antibody to TLR2 and TLR4 (1:400, BA1716/BA1717, Boster Biological Technology, China), rabbit polyclonal antibody to NF-κB (1:1000; sc-109, Santacruz, CA, USA), rabbit monoclonal antibody to iNOS (1:500, bs0162R, Bioss, China), rabbit monoclonal antibody to COX-2 (1:500, 55070-1-AP, Proteintech, China,) and mouse monoclonal antibody to β-actin (1:10,000, D110007-0100, Songon Biotech, China). .. The next day, the blots were washed and incubated for 1 h with respective HRP-conjugated secondary antibodies (ZSGB-BIO, Beijing, China; dilution 1:3000) at room temperature.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor κB and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis
    Article Snippet: .. Materials TLR4 antibody and NF-κB antibody (Boster, Wuhan, China), NF-κB p65 antibody (Ebioscience, USA), BCA protein concentration Kit (Pierce, USA), reverse transcription polymerase chain reaction (RT-PCR) Kit (Gibco, USA), TRIzol (Invitrogen, USA), PCR primer and DNA ladder (Sbsgene, China). .. Animal grouping and ANP model development Seventy two male SD rats were divided into three groups with 24 rats each as sham-operation, ANP, and ANP with PDTC pretreatment.

    Incubation:

    Article Title: Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens
    Article Snippet: .. The membranes were blocked with blocking buffer (Beyotime) at room temperature for 2 h and then were incubated with anti-phospho-P38 [#4511T, anti-rabbit, Cell Signaling Technology (CST), USA], anti-P38 (#9212S, anti-rabbit, CST), anti-phospho-JNK (#4668S, anti-rabbit, CST), anti-JNK (#928, anti-rabbit, CST), anti-phospho-ERK (#9101S, anti-rabbit, CST), anti-ERK (#9102S, anti-rabbit, CST), anti-TLR-3 (NBP2-24565, anti-rabbit, NBP2-24565Novus Biologicals, USA), and anti-TLR-4 (BA1717, anti-rabbit, Boster, China) primary antibodies overnight at 4°C, followed by incubation with the corresponding horseradish peroxidase-conjugated secondary antibody (Beyotime) at 4°C for 4 h. The protein–antibody complexes were detected with the ECL Plus A and B (Beyotime), and the results were quantified using the Fusion FX software (Vilber, France). .. Nuclear and cytoplasmic proteins were prepared using the nuclear and cytoplasmic protein extraction kit (Beyotime), and then the levels of NF-κB p65 (D14E12) subunit (#3033P, anti-rabbit, CST), nuclear factor erythroid 2 p45-related factor 2 (Nrf2) (ab62352, anti-rabbit; Abcam, Britain), and inhibitory protein-κBα (IκBα) (44D4) (#4812S, anti-rabbit, CST) were detected.

    Article Title: H19 promote calcium oxalate nephrocalcinosis-induced renal tubular epithelial cell injury via a ceRNA pathway
    Article Snippet: .. For IHC, sections were incubated overnight at 4 °C with anti-HMGB1, anti-TLR4, anti-NF-kB, anti-SOD2 (Boster, China, BM4813) and anti-NOX2 (Boster, China, BA2811) antibodies and then detected using an Envision HRP Polymer System (Boster, China). .. All images were acquired by a high-throughput Leica SCN400 scanner.

    Article Title: Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling
    Article Snippet: .. The membranes were incubated against rabbit anti-TLR4 (A00017-Boster, 1:400) and anti-human β-actin (sc-47778-Santa Cruz, 1:1000) antibodies for 12 h and after washing in 1X TBST buffer thrice, were incubated with peroxidase conjugated secondary antibody (1:3000) for 30 min (Boster, China). .. The blots were developed with Super Signal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA) and visualized using ChemiDoc-It™ Imaging System.

    Protein Concentration:

    Article Title: Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor κB and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis
    Article Snippet: .. Materials TLR4 antibody and NF-κB antibody (Boster, Wuhan, China), NF-κB p65 antibody (Ebioscience, USA), BCA protein concentration Kit (Pierce, USA), reverse transcription polymerase chain reaction (RT-PCR) Kit (Gibco, USA), TRIzol (Invitrogen, USA), PCR primer and DNA ladder (Sbsgene, China). .. Animal grouping and ANP model development Seventy two male SD rats were divided into three groups with 24 rats each as sham-operation, ANP, and ANP with PDTC pretreatment.

    Polymerase Chain Reaction:

    Article Title: Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor κB and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis
    Article Snippet: .. Materials TLR4 antibody and NF-κB antibody (Boster, Wuhan, China), NF-κB p65 antibody (Ebioscience, USA), BCA protein concentration Kit (Pierce, USA), reverse transcription polymerase chain reaction (RT-PCR) Kit (Gibco, USA), TRIzol (Invitrogen, USA), PCR primer and DNA ladder (Sbsgene, China). .. Animal grouping and ANP model development Seventy two male SD rats were divided into three groups with 24 rats each as sham-operation, ANP, and ANP with PDTC pretreatment.

    Software:

    Article Title: Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens
    Article Snippet: .. The membranes were blocked with blocking buffer (Beyotime) at room temperature for 2 h and then were incubated with anti-phospho-P38 [#4511T, anti-rabbit, Cell Signaling Technology (CST), USA], anti-P38 (#9212S, anti-rabbit, CST), anti-phospho-JNK (#4668S, anti-rabbit, CST), anti-JNK (#928, anti-rabbit, CST), anti-phospho-ERK (#9101S, anti-rabbit, CST), anti-ERK (#9102S, anti-rabbit, CST), anti-TLR-3 (NBP2-24565, anti-rabbit, NBP2-24565Novus Biologicals, USA), and anti-TLR-4 (BA1717, anti-rabbit, Boster, China) primary antibodies overnight at 4°C, followed by incubation with the corresponding horseradish peroxidase-conjugated secondary antibody (Beyotime) at 4°C for 4 h. The protein–antibody complexes were detected with the ECL Plus A and B (Beyotime), and the results were quantified using the Fusion FX software (Vilber, France). .. Nuclear and cytoplasmic proteins were prepared using the nuclear and cytoplasmic protein extraction kit (Beyotime), and then the levels of NF-κB p65 (D14E12) subunit (#3033P, anti-rabbit, CST), nuclear factor erythroid 2 p45-related factor 2 (Nrf2) (ab62352, anti-rabbit; Abcam, Britain), and inhibitory protein-κBα (IκBα) (44D4) (#4812S, anti-rabbit, CST) were detected.

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  • 92
    Boster Bio rabbit anti tlr4 antibody
    Quantitative RT-PCR shows the expression of <t>Tlr4</t> and glia-related genes in cervical spinal cord dorsal horn (C3–C4) after AEW treatment on the back skin or intrathecal LPS injection
    Rabbit Anti Tlr4 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tlr4 antibody/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti tlr4 antibody - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    94
    Boster Bio anti tlr4
    Effects of long-term EGCG treatment on hippocampal expression of <t>TLR4,</t> NF-κB, and IL-1β. ( A, C, E ) Representative Western blot images of TLR 4, NF-κB, and IL-1β in the control, EP, and EP+EGCG groups. ( B, D, F ) Histograms showing the quantitative analyses of TLR 4, NF-κB, and IL-1β. Data are expressed as the means ±SEM. (n=6/group). * P
    Anti Tlr4, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tlr4/product/Boster Bio
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti tlr4 - by Bioz Stars, 2021-01
    94/100 stars
      Buy from Supplier

    Image Search Results


    Quantitative RT-PCR shows the expression of Tlr4 and glia-related genes in cervical spinal cord dorsal horn (C3–C4) after AEW treatment on the back skin or intrathecal LPS injection

    Journal: Pain

    Article Title: Toll-like receptor 4 contributes to chronic itch, alloknesis and spinal astrocyte activation in male mice

    doi: 10.1097/j.pain.0000000000000439

    Figure Lengend Snippet: Quantitative RT-PCR shows the expression of Tlr4 and glia-related genes in cervical spinal cord dorsal horn (C3–C4) after AEW treatment on the back skin or intrathecal LPS injection

    Article Snippet: Briefly, the tissue sections were blocked with 2% goat serum, and incubated over night at 4°C with the primary antibodies: mouse GFAP antibody (1:2000, Millipore), rabbit IBA-1 antibody (1:5000, Wako), rabbit anti-TLR4 antibody (1:200, Boster, China).

    Techniques: Quantitative RT-PCR, Expressing, Injection

    Alloknesis under both acute and chronic itch conditions is impaired in Tlr4 −/− mice and suppressed by intrathecal LPS-RS in the back and cheek models

    Journal: Pain

    Article Title: Toll-like receptor 4 contributes to chronic itch, alloknesis and spinal astrocyte activation in male mice

    doi: 10.1097/j.pain.0000000000000439

    Figure Lengend Snippet: Alloknesis under both acute and chronic itch conditions is impaired in Tlr4 −/− mice and suppressed by intrathecal LPS-RS in the back and cheek models

    Article Snippet: Briefly, the tissue sections were blocked with 2% goat serum, and incubated over night at 4°C with the primary antibodies: mouse GFAP antibody (1:2000, Millipore), rabbit IBA-1 antibody (1:5000, Wako), rabbit anti-TLR4 antibody (1:200, Boster, China).

    Techniques: Mouse Assay

    AEW treatment for 5 days in the back model induces astrogliosis but not microgliosis in cervical spinal cord dorsal horn (C3–C4) via TLR4

    Journal: Pain

    Article Title: Toll-like receptor 4 contributes to chronic itch, alloknesis and spinal astrocyte activation in male mice

    doi: 10.1097/j.pain.0000000000000439

    Figure Lengend Snippet: AEW treatment for 5 days in the back model induces astrogliosis but not microgliosis in cervical spinal cord dorsal horn (C3–C4) via TLR4

    Article Snippet: Briefly, the tissue sections were blocked with 2% goat serum, and incubated over night at 4°C with the primary antibodies: mouse GFAP antibody (1:2000, Millipore), rabbit IBA-1 antibody (1:5000, Wako), rabbit anti-TLR4 antibody (1:200, Boster, China).

    Techniques:

    AEW treatment for 5 days in the back model increases TLR4 expression in spinal cord astrocytes

    Journal: Pain

    Article Title: Toll-like receptor 4 contributes to chronic itch, alloknesis and spinal astrocyte activation in male mice

    doi: 10.1097/j.pain.0000000000000439

    Figure Lengend Snippet: AEW treatment for 5 days in the back model increases TLR4 expression in spinal cord astrocytes

    Article Snippet: Briefly, the tissue sections were blocked with 2% goat serum, and incubated over night at 4°C with the primary antibodies: mouse GFAP antibody (1:2000, Millipore), rabbit IBA-1 antibody (1:5000, Wako), rabbit anti-TLR4 antibody (1:200, Boster, China).

    Techniques: Expressing

    TLR4 signaling is required for chronic itch but not acute itch in the back and cheek models

    Journal: Pain

    Article Title: Toll-like receptor 4 contributes to chronic itch, alloknesis and spinal astrocyte activation in male mice

    doi: 10.1097/j.pain.0000000000000439

    Figure Lengend Snippet: TLR4 signaling is required for chronic itch but not acute itch in the back and cheek models

    Article Snippet: Briefly, the tissue sections were blocked with 2% goat serum, and incubated over night at 4°C with the primary antibodies: mouse GFAP antibody (1:2000, Millipore), rabbit IBA-1 antibody (1:5000, Wako), rabbit anti-TLR4 antibody (1:200, Boster, China).

    Techniques:

    Effects of long-term EGCG treatment on hippocampal expression of TLR4, NF-κB, and IL-1β. ( A, C, E ) Representative Western blot images of TLR 4, NF-κB, and IL-1β in the control, EP, and EP+EGCG groups. ( B, D, F ) Histograms showing the quantitative analyses of TLR 4, NF-κB, and IL-1β. Data are expressed as the means ±SEM. (n=6/group). * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: (−)-Epigallocatechin-3-Gallate Protects Against Lithium-Pilocarpine-Induced Epilepsy by Inhibiting the Toll-Like Receptor 4 (TLR4)/Nuclear Factor-κB (NF-κB) Signaling Pathway

    doi: 10.12659/MSM.915025

    Figure Lengend Snippet: Effects of long-term EGCG treatment on hippocampal expression of TLR4, NF-κB, and IL-1β. ( A, C, E ) Representative Western blot images of TLR 4, NF-κB, and IL-1β in the control, EP, and EP+EGCG groups. ( B, D, F ) Histograms showing the quantitative analyses of TLR 4, NF-κB, and IL-1β. Data are expressed as the means ±SEM. (n=6/group). * P

    Article Snippet: The PVDF membrane was then placed in 5% nonfat milk powder and sealed at room temperature for 1 h. Antibodies were diluted with PBS solution at the appropriate concentration according to the specification of each antibody, anti-IL-1β (1: 800 Bioworld USA), anti-TLR4 (1: 500 Boster China), anti-NF-κB (1: 1000 Bioworld USA), and anti-β-actin (1: 1000 Santa Cruz, CA, USA).

    Techniques: Expressing, Western Blot

    The effect of ischemic postconditioning on TLR4 between 2 hour and 4.5 hour ischemia groups. (A) Representative images of cells with positive immunochemical staining to TLR4, scale bar = 50 μm. (B) Statistics of the number of cells stained with TLR4. (C) Statistics of the transcriptional levels of TLR4. (D) Representative Western blotting images of TLR4. (E) Statistics of the expressional level of TLR4. (F) Representative images under confocal microscope in the sham group. (G) Representative images under confocal microscope in the ischemia group; (n = 4 in each group).

    Journal: Journal of Neuroinflammation

    Article Title: Protection of ischemic post conditioning against transient focal ischemia-induced brain damage is associated with inhibition of neuroinflammation via modulation of TLR2 and TLR4 pathways

    doi: 10.1186/1742-2094-11-15

    Figure Lengend Snippet: The effect of ischemic postconditioning on TLR4 between 2 hour and 4.5 hour ischemia groups. (A) Representative images of cells with positive immunochemical staining to TLR4, scale bar = 50 μm. (B) Statistics of the number of cells stained with TLR4. (C) Statistics of the transcriptional levels of TLR4. (D) Representative Western blotting images of TLR4. (E) Statistics of the expressional level of TLR4. (F) Representative images under confocal microscope in the sham group. (G) Representative images under confocal microscope in the ischemia group; (n = 4 in each group).

    Article Snippet: The membranes were probed with anti-TLR4 (1:1,000, Boster Biotechnology Company, Wuhan, China), anti-TLR2 (1:1,000, Epitomics), anti-IRAK4 (1:1,000, Cell signaling), anti-IL-1β (1:1,000, Proteintech), anti-β-actin (1:2,000, Proteintech).

    Techniques: Staining, Western Blot, Microscopy

    Functional pathway analysis and graphical illustration of molecular mechanism of LPS induced bursal atrophy A. Diagram showing TLR4-MAPK-NF-κB/AP-1 pathway analysis of differentially regulated genes in LPS-treated BF. The red colored text represents upregulated genes; blue designates downregulated genes and black designates neighboring genes in TLR4-MAPK-NF-κB/AP-1 pathway in chicken bursa of Fabricius at early time point. The figure was drawn in Pathway Builder Tool. 2 software. B. Representative images of immunostained BF sections from LPS and saline control group chicks using anti-TLR4 antibody at 12, 36 and 72 h time points. As seen, TLR4 expression is enhanced in the LPS treated BF compared to control. Integral optical density (IOD) shows increased TLR4 expression at 12 and 36 h after LPS treatment. Scale bars = 50μm. Note: * denotes P

    Journal: Oncotarget

    Article Title: Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling

    doi: 10.18632/oncotarget.19964

    Figure Lengend Snippet: Functional pathway analysis and graphical illustration of molecular mechanism of LPS induced bursal atrophy A. Diagram showing TLR4-MAPK-NF-κB/AP-1 pathway analysis of differentially regulated genes in LPS-treated BF. The red colored text represents upregulated genes; blue designates downregulated genes and black designates neighboring genes in TLR4-MAPK-NF-κB/AP-1 pathway in chicken bursa of Fabricius at early time point. The figure was drawn in Pathway Builder Tool. 2 software. B. Representative images of immunostained BF sections from LPS and saline control group chicks using anti-TLR4 antibody at 12, 36 and 72 h time points. As seen, TLR4 expression is enhanced in the LPS treated BF compared to control. Integral optical density (IOD) shows increased TLR4 expression at 12 and 36 h after LPS treatment. Scale bars = 50μm. Note: * denotes P

    Article Snippet: The membranes were incubated against rabbit anti-TLR4 (A00017-Boster, 1:400) and anti-human β-actin (sc-47778-Santa Cruz, 1:1000) antibodies for 12 h and after washing in 1X TBST buffer thrice, were incubated with peroxidase conjugated secondary antibody (1:3000) for 30 min (Boster, China).

    Techniques: Functional Assay, Software, Expressing