rabbit polyclonal primary antibody  (Synaptic Systems)

Journal: In Vivo

Article Title: Therapeutic Potential of Dimethyl Sulfoxide via Subconjunctival Injection in a Diabetic Retinopathy Rat Model

doi: 10.21873/invivo.13811

Figure Lengend Snippet: GFAP immunohistochemical staining of the rat retina in CON, DMSO 0, and DMSO 50 groups. Sections of a control (A), DMSO 0 (B), and the 50% DMSO treatment group (C) retina were stained with anti-GFAP antibodies using 3.3’-diaminobenzidine (DAB: brown) as the chromogen. Nuclei were counterstained with hematoxylin (blue). Bar=100 μm.

Article Snippet: To determine the anti-inflammatory effect of 50% DMSO based on the expression of glial fibrillary acidic protein (GFAP) in retinal slices, the retinal tissues were rehydrated and then incubated with rabbit polyclonal anti-GFAP primary antibodies (1:1,000, ab7260, Abcam, Cambridge, UK) for 1 h and 30 min at 26˚C.

Techniques: Immunohistochemical staining, Staining, Control

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Identification of immune suppressor candidates utilizing comparative transcriptional profiling in histiocytic sarcoma

doi: 10.1007/s00262-024-03908-x

Figure Lengend Snippet: Immunostaining of canine and human histiocytic sarcoma (HS) for osteopontin. Formalin-fixed paraffin-embedded tissues from 18 canine HS and five human HS were stained for osteopontin expression. Slides scanned using an Aperio AT2 automated slide scanner (Leica Biosystems) were visualized using ImageScope software (Leica Biosystems) and an algorithm for quantifying positive pixels was applied to non-necrotic areas of the entirety of the outlined tumor area. Photomicrographs of representative canine HS with primary tissue of origin of A lymph node (LN), B lung, C peri-articular (PA) tissues and D spleen. E Quantification of osteopontin staining grouped by anatomic location in 18 canine HS samples. Each dot represents an individual tumor, and the bar represents the anatomic mean. Photomicrographs of human tumors from five patients diagnosed with HS in F the brain, G subcutaneous tissue of the buttock, H subcutaneous tissue of the foot, I spleen and J tonsil. K Quantification of osteopontin staining grouped by species. Each dot represents an individual tumor, and the bar represents the species mean. Scale bars measure 200 μm

Article Snippet: Osteopontin was detected in both canine and human sections using primary polyclonal rabbit anti-human osteopontin antibody (Rockland Immunochemicals).

Techniques: Immunostaining, Formalin-fixed Paraffin-Embedded, Staining, Expressing, Software