rabbit polyclonal p2x7 c terminus antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal p2x7 c terminus antibody
    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p
    Rabbit Polyclonal P2x7 C Terminus Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal p2x7 c terminus antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal p2x7 c terminus antibody - by Bioz Stars, 2022-09
    86/100 stars

    Images

    1) Product Images from "Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *"

    Article Title: Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.574699

    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p
    Figure Legend Snippet: The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p

    Techniques Used: Activity Assay, Sequencing, Mutagenesis, Relative Rate

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    Alomone Labs rabbit polyclonal p2x7 c terminus antibody
    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p
    Rabbit Polyclonal P2x7 C Terminus Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal p2x7 c terminus antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal p2x7 c terminus antibody - by Bioz Stars, 2022-09
    86/100 stars
      Buy from Supplier

    96
    Alomone Labs rabbit anti p2x7
    Silencing nc021972 prevented the activation of the <t>P2X7</t> receptor induced by HGHF via p38 MAPK signaling in PC12 cells. The protein level of phosphorylation of p38 was tested by Western blotting analysis. Representative images of Western blotting ( a ).
    Rabbit Anti P2x7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti p2x7/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti p2x7 - by Bioz Stars, 2022-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in  gray , P2X1 aa20–23 and equivalent residues in P2X2  underlined , and the P2X7 residues Lys 17  and Val 18  in  red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7.  C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT:  n  = 14 alone,  n  = 11 MCD,  n  = 3 cholesterol; mutants:  n  = 12 alone,  n  = 11 MCD,  n  = 3 cholesterol; *,  p

    Journal: The Journal of Biological Chemistry

    Article Title: Plasma Membrane Cholesterol as a Regulator of Human and Rodent P2X7 Receptor Activation and Sensitization *

    doi: 10.1074/jbc.M114.574699

    Figure Lengend Snippet: The N terminus is important for the sensitivity of P2X7 receptor activity to cholesterol depletion. A , amino acid sequence alignment of the N-terminal region of human P2X1, P2X2, and P2X7 receptors, with conserved amino acids highlighted in gray , P2X1 aa20–23 and equivalent residues in P2X2 underlined , and the P2X7 residues Lys 17 and Val 18 in red. B , time course of dye uptake mediated by the K17R/V18M mutant and wild type P2X7, alone, pretreated with MCD and loaded with cholesterol ( chol ), normalized to the untreated WT P2X7. C , bar graph of the relative rate of dye uptake normalized to the untreated WT P2X7. Dye uptake mediated by the K17R/V18M mutant is not potentiated by MCD, but is inhibited by cholesterol loading (WT: n = 14 alone, n = 11 MCD, n = 3 cholesterol; mutants: n = 12 alone, n = 11 MCD, n = 3 cholesterol; *, p

    Article Snippet: Rabbit polyclonal P2X7 C terminus antibody was obtained from Alomone Labs (Jerusalem, Israel), and anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were from Thermo Fisher Scientific and Bio-Rad, respectively.

    Techniques: Activity Assay, Sequencing, Mutagenesis, Relative Rate

    Silencing nc021972 prevented the activation of the P2X7 receptor induced by HGHF via p38 MAPK signaling in PC12 cells. The protein level of phosphorylation of p38 was tested by Western blotting analysis. Representative images of Western blotting ( a ).

    Journal: Purinergic Signalling

    Article Title: LncRNA NONRATT021972 siRNA attenuates P2X7 receptor expression and inflammatory cytokine production induced by combined high glucose and free fatty acids in PC12 cells

    doi: 10.1007/s11302-016-9500-0

    Figure Lengend Snippet: Silencing nc021972 prevented the activation of the P2X7 receptor induced by HGHF via p38 MAPK signaling in PC12 cells. The protein level of phosphorylation of p38 was tested by Western blotting analysis. Representative images of Western blotting ( a ).

    Article Snippet: The rabbit anti-P2X7 (corresponding to amino acid residues 576–595 of the rat P2X7 receptor, C-terminus) was bought from Alomone Laboratories (Jerusalem, Israel).

    Techniques: Activation Assay, Western Blot

    Silencing nc021972 suppressed the activation of the P2X7 receptor evoked by HGHF in PC12 cells. a mRNA expression of the P2X7 receptor determined by real-time PCR. The protein level of the P2X7 receptor was tested by Western blotting analysis. Representative

    Journal: Purinergic Signalling

    Article Title: LncRNA NONRATT021972 siRNA attenuates P2X7 receptor expression and inflammatory cytokine production induced by combined high glucose and free fatty acids in PC12 cells

    doi: 10.1007/s11302-016-9500-0

    Figure Lengend Snippet: Silencing nc021972 suppressed the activation of the P2X7 receptor evoked by HGHF in PC12 cells. a mRNA expression of the P2X7 receptor determined by real-time PCR. The protein level of the P2X7 receptor was tested by Western blotting analysis. Representative

    Article Snippet: The rabbit anti-P2X7 (corresponding to amino acid residues 576–595 of the rat P2X7 receptor, C-terminus) was bought from Alomone Laboratories (Jerusalem, Israel).

    Techniques: Activation Assay, Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Silencing nc021972 prevented P2X7 receptor-mediated PC12 neuroinflammation and cell viability induced by HGHF in PC12 cells. Basal and BzATP-induced IL-6 ( a ) and TNF-α ( b ) release and cell viability of PC12 cells ( c ) were recorded after tranfection

    Journal: Purinergic Signalling

    Article Title: LncRNA NONRATT021972 siRNA attenuates P2X7 receptor expression and inflammatory cytokine production induced by combined high glucose and free fatty acids in PC12 cells

    doi: 10.1007/s11302-016-9500-0

    Figure Lengend Snippet: Silencing nc021972 prevented P2X7 receptor-mediated PC12 neuroinflammation and cell viability induced by HGHF in PC12 cells. Basal and BzATP-induced IL-6 ( a ) and TNF-α ( b ) release and cell viability of PC12 cells ( c ) were recorded after tranfection

    Article Snippet: The rabbit anti-P2X7 (corresponding to amino acid residues 576–595 of the rat P2X7 receptor, C-terminus) was bought from Alomone Laboratories (Jerusalem, Israel).

    Techniques: