Structured Review

Santa Cruz Biotechnology rabbit polyclonal antibodies against p22 phox
Nox-4 (a) and <t>p22</t> <t>phox</t> (b) protein expressions in the liver. m/m : Misty; Veh: vehicle-treated db/db mice; K-100: Kangen-karyu 100 mg/kg body weight-treated db/db mice; K-200: Kangen-karyu 200 mg/kg body weight-treated db/db mice. Bars represent the means ± S.E.M. * P < 0.05, ** P < 0.01, *** P < 0.001 versus vehicle-treated db/db mouse values.
Rabbit Polyclonal Antibodies Against P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibodies against p22 phox/product/Santa Cruz Biotechnology
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal antibodies against p22 phox - by Bioz Stars, 2024-03
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1) Product Images from "Evaluation of Effects of Chinese Prescription Kangen-karyu on Diabetes-Induced Alterations such as Oxidative Stress and Apoptosis in the Liver of Type 2 Diabetic db/db Mice"

Article Title: Evaluation of Effects of Chinese Prescription Kangen-karyu on Diabetes-Induced Alterations such as Oxidative Stress and Apoptosis in the Liver of Type 2 Diabetic db/db Mice

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2012/143489

Nox-4 (a) and p22 phox (b) protein expressions in the liver. m/m : Misty; Veh: vehicle-treated db/db mice; K-100: Kangen-karyu 100 mg/kg body weight-treated db/db mice; K-200: Kangen-karyu 200 mg/kg body weight-treated db/db mice. Bars represent the means ± S.E.M. * P < 0.05, ** P < 0.01, *** P < 0.001 versus vehicle-treated db/db mouse values.
Figure Legend Snippet: Nox-4 (a) and p22 phox (b) protein expressions in the liver. m/m : Misty; Veh: vehicle-treated db/db mice; K-100: Kangen-karyu 100 mg/kg body weight-treated db/db mice; K-200: Kangen-karyu 200 mg/kg body weight-treated db/db mice. Bars represent the means ± S.E.M. * P < 0.05, ** P < 0.01, *** P < 0.001 versus vehicle-treated db/db mouse values.

Techniques Used:


Structured Review

Santa Cruz Biotechnology rabbit polyclonal anti p22 phox
Rabbit Polyclonal Anti P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p22 phox/product/Santa Cruz Biotechnology
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti p22 phox - by Bioz Stars, 2024-03
95/100 stars

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Structured Review

Santa Cruz Biotechnology rabbit polyclonal antibodies against p22 phox
NADPH oxidase subunit <t>p22</t> <t>phox</t> expression. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for 24 hours or 48 hours, or pretreated with 10 μ M rosiglitazone (RSG), or 10 μ M ciglitazone or 10 μ M troglitazone) and p22 phox was detected by immunoblotting. Pretreatment with three different PPAR γ agonists blocked HG-induced p22 phox expression ( n = 5, * P < .05 versus NG, ** P < .05 versus HG 24 hours or HG 48 hours).
Rabbit Polyclonal Antibodies Against P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibodies against p22 phox/product/Santa Cruz Biotechnology
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal antibodies against p22 phox - by Bioz Stars, 2024-03
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1) Product Images from "Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells"

Article Title: Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells

Journal: Experimental Diabetes Research

doi: 10.1155/2009/910783

NADPH oxidase subunit p22 phox expression. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for 24 hours or 48 hours, or pretreated with 10 μ M rosiglitazone (RSG), or 10 μ M ciglitazone or 10 μ M troglitazone) and p22 phox was detected by immunoblotting. Pretreatment with three different PPAR γ agonists blocked HG-induced p22 phox expression ( n = 5, * P < .05 versus NG, ** P < .05 versus HG 24 hours or HG 48 hours).
Figure Legend Snippet: NADPH oxidase subunit p22 phox expression. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for 24 hours or 48 hours, or pretreated with 10 μ M rosiglitazone (RSG), or 10 μ M ciglitazone or 10 μ M troglitazone) and p22 phox was detected by immunoblotting. Pretreatment with three different PPAR γ agonists blocked HG-induced p22 phox expression ( n = 5, * P < .05 versus NG, ** P < .05 versus HG 24 hours or HG 48 hours).

Techniques Used: Expressing, Western Blot


Structured Review

Santa Cruz Biotechnology rabbit polyclonal anti p22 phox
(A) Expression of the NADPH oxidase subunits Nox4 and <t>p22</t> <t>phox</t> in the renal cortex of young and old WKY rats. GAPDH was used to control for loading differences. (B) The bar graph shows a summary of densitometric data. Values are normalized to the level of GAPDH expression in each condition and expressed as % of young rats. Each column represents the mean ± SEM of six independent immunoblots. Significantly different from values in young rats (*p < 0.05) using the Newman-Keuls test.
Rabbit Polyclonal Anti P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p22 phox/product/Santa Cruz Biotechnology
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1) Product Images from "Aging increases oxidative stress and renal expression of oxidant and antioxidant enzymes that are associated with an increased trend in systolic blood pressure"

Article Title: Aging increases oxidative stress and renal expression of oxidant and antioxidant enzymes that are associated with an increased trend in systolic blood pressure

Journal: Oxidative Medicine and Cellular Longevity

doi:

(A) Expression of the NADPH oxidase subunits Nox4 and p22 phox in the renal cortex of young and old WKY rats. GAPDH was used to control for loading differences. (B) The bar graph shows a summary of densitometric data. Values are normalized to the level of GAPDH expression in each condition and expressed as % of young rats. Each column represents the mean ± SEM of six independent immunoblots. Significantly different from values in young rats (*p < 0.05) using the Newman-Keuls test.
Figure Legend Snippet: (A) Expression of the NADPH oxidase subunits Nox4 and p22 phox in the renal cortex of young and old WKY rats. GAPDH was used to control for loading differences. (B) The bar graph shows a summary of densitometric data. Values are normalized to the level of GAPDH expression in each condition and expressed as % of young rats. Each column represents the mean ± SEM of six independent immunoblots. Significantly different from values in young rats (*p < 0.05) using the Newman-Keuls test.

Techniques Used: Expressing, Western Blot


Structured Review

Santa Cruz Biotechnology rabbit polyclonal p22 phox
Expression of NADPH oxidase subunits: (a) gp91 <t>phox</t> , (b) <t>p22</t> phox , (c) NOX4, (d) p47phox, and (e) p67 phox . Group I ( n = 6) was sham-operated animals, which underwent the same surgical procedure without vessel occlusion (control), group II ( n = 6) was subjected to ischemia/reperfusion (I/R), and group III ( n = 5) was treated with vitamin D3 and then subjected to I/R (VitD + I/R). All experiments were performed in synaptosomal fraction in different brain structures. Results are presented as mean ± SE. ∗ p < 0.05 versus control, ∗∗ p < 0.01 versus control, § p < 0.05 versus I/R, and §§ p < 0.01 versus I/R.
Rabbit Polyclonal P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal p22 phox/product/Santa Cruz Biotechnology
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1) Product Images from "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia"

Article Title: Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2018/3273654

Expression of NADPH oxidase subunits: (a) gp91 phox , (b) p22 phox , (c) NOX4, (d) p47phox, and (e) p67 phox . Group I ( n = 6) was sham-operated animals, which underwent the same surgical procedure without vessel occlusion (control), group II ( n = 6) was subjected to ischemia/reperfusion (I/R), and group III ( n = 5) was treated with vitamin D3 and then subjected to I/R (VitD + I/R). All experiments were performed in synaptosomal fraction in different brain structures. Results are presented as mean ± SE. ∗ p < 0.05 versus control, ∗∗ p < 0.01 versus control, § p < 0.05 versus I/R, and §§ p < 0.01 versus I/R.
Figure Legend Snippet: Expression of NADPH oxidase subunits: (a) gp91 phox , (b) p22 phox , (c) NOX4, (d) p47phox, and (e) p67 phox . Group I ( n = 6) was sham-operated animals, which underwent the same surgical procedure without vessel occlusion (control), group II ( n = 6) was subjected to ischemia/reperfusion (I/R), and group III ( n = 5) was treated with vitamin D3 and then subjected to I/R (VitD + I/R). All experiments were performed in synaptosomal fraction in different brain structures. Results are presented as mean ± SE. ∗ p < 0.05 versus control, ∗∗ p < 0.01 versus control, § p < 0.05 versus I/R, and §§ p < 0.01 versus I/R.

Techniques Used: Expressing

Immunohistochemical staining of gp91 phox and p22 phox in gerbil brain slices. Magnification of images for (a) from A–F is 200×, for (b) from A–C is 40×, and for (b) from D–I is 100×.
Figure Legend Snippet: Immunohistochemical staining of gp91 phox and p22 phox in gerbil brain slices. Magnification of images for (a) from A–F is 200×, for (b) from A–C is 40×, and for (b) from D–I is 100×.

Techniques Used: Immunohistochemical staining, Staining


Structured Review

Santa Cruz Biotechnology polyclonal rabbit anti p22 phox antibody
Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase state in WT and S100A9 −/− differentiated Hoxb8 cells. ( a ) Expression of the NADPH oxidase subunits. gp91 <t>phox</t> , p67 phox , p47 phox , p40 phox and <t>p22</t> phox expression in non-differentiated (d0) and differentiated (d5) WT or S100A9 −/− Hoxb8 cells was determined by Western Blot. A representative Western Blot of three independent experiments is shown. ( b ) reactive oxygen species (ROS) production stimulated by N-Formylmethionyl-leucyl-phenylalanine (fMLF), phorbolmyristate acetate (PMA) or lipopolysaccharide (LPS). Differentiated WT and S100A9 −/− Hoxb8 cells were stimulated with fMLF (100 nM), PMA (100 nM) or LPS (100 ng/mL). Total ROS production was measured on a plate reader by real-time chemiluminescence. The results represent fold induction of ROS production of S100A9 −/− Hoxb8 cells compared to S100A9 −/− Hoxb8 cells and are expressed as mean ± SEM. Inserts: representative traces showing ROS production in WT Hoxb8 cells stimulated or not with fMLF, PMA or LPS. Significantly different from control * = p < 0.05, ** = p < 0.01.
Polyclonal Rabbit Anti P22 Phox Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal rabbit anti p22 phox antibody/product/Santa Cruz Biotechnology
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polyclonal rabbit anti p22 phox antibody - by Bioz Stars, 2024-03
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1) Product Images from "Role of S100A8/A9 for Cytokine Secretion, Revealed in Neutrophils Derived from ER-Hoxb8 Progenitors"

Article Title: Role of S100A8/A9 for Cytokine Secretion, Revealed in Neutrophils Derived from ER-Hoxb8 Progenitors

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms22168845

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase state in WT and S100A9 −/− differentiated Hoxb8 cells. ( a ) Expression of the NADPH oxidase subunits. gp91 phox , p67 phox , p47 phox , p40 phox and p22 phox expression in non-differentiated (d0) and differentiated (d5) WT or S100A9 −/− Hoxb8 cells was determined by Western Blot. A representative Western Blot of three independent experiments is shown. ( b ) reactive oxygen species (ROS) production stimulated by N-Formylmethionyl-leucyl-phenylalanine (fMLF), phorbolmyristate acetate (PMA) or lipopolysaccharide (LPS). Differentiated WT and S100A9 −/− Hoxb8 cells were stimulated with fMLF (100 nM), PMA (100 nM) or LPS (100 ng/mL). Total ROS production was measured on a plate reader by real-time chemiluminescence. The results represent fold induction of ROS production of S100A9 −/− Hoxb8 cells compared to S100A9 −/− Hoxb8 cells and are expressed as mean ± SEM. Inserts: representative traces showing ROS production in WT Hoxb8 cells stimulated or not with fMLF, PMA or LPS. Significantly different from control * = p < 0.05, ** = p < 0.01.
Figure Legend Snippet: Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase state in WT and S100A9 −/− differentiated Hoxb8 cells. ( a ) Expression of the NADPH oxidase subunits. gp91 phox , p67 phox , p47 phox , p40 phox and p22 phox expression in non-differentiated (d0) and differentiated (d5) WT or S100A9 −/− Hoxb8 cells was determined by Western Blot. A representative Western Blot of three independent experiments is shown. ( b ) reactive oxygen species (ROS) production stimulated by N-Formylmethionyl-leucyl-phenylalanine (fMLF), phorbolmyristate acetate (PMA) or lipopolysaccharide (LPS). Differentiated WT and S100A9 −/− Hoxb8 cells were stimulated with fMLF (100 nM), PMA (100 nM) or LPS (100 ng/mL). Total ROS production was measured on a plate reader by real-time chemiluminescence. The results represent fold induction of ROS production of S100A9 −/− Hoxb8 cells compared to S100A9 −/− Hoxb8 cells and are expressed as mean ± SEM. Inserts: representative traces showing ROS production in WT Hoxb8 cells stimulated or not with fMLF, PMA or LPS. Significantly different from control * = p < 0.05, ** = p < 0.01.

Techniques Used: Expressing, Western Blot


Structured Review

Santa Cruz Biotechnology rabbit polyclonal anti p22 phox
Rabbit Polyclonal Anti P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p22 phox/product/Santa Cruz Biotechnology
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti p22 phox - by Bioz Stars, 2024-03
95/100 stars

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Santa Cruz Biotechnology rabbit polyclonal anti p22 phox
Rabbit Polyclonal Anti P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p22 phox/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti p22 phox - by Bioz Stars, 2024-03
86/100 stars

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Structured Review

Santa Cruz Biotechnology rabbit polyclonal anti p22 phox
Rabbit Polyclonal Anti P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p22 phox/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti p22 phox - by Bioz Stars, 2024-03
86/100 stars

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Santa Cruz Biotechnology rabbit polyclonal anti p22 phox
Rabbit Polyclonal Anti P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p22 phox/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti p22 phox - by Bioz Stars, 2024-03
86/100 stars

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  • 95
    Santa Cruz Biotechnology rabbit polyclonal antibodies against p22 phox
    Nox-4 (a) and <t>p22</t> <t>phox</t> (b) protein expressions in the liver. m/m : Misty; Veh: vehicle-treated db/db mice; K-100: Kangen-karyu 100 mg/kg body weight-treated db/db mice; K-200: Kangen-karyu 200 mg/kg body weight-treated db/db mice. Bars represent the means ± S.E.M. * P < 0.05, ** P < 0.01, *** P < 0.001 versus vehicle-treated db/db mouse values.
    Rabbit Polyclonal Antibodies Against P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against p22 phox/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibodies against p22 phox - by Bioz Stars, 2024-03
    95/100 stars
      Buy from Supplier

    95
    Santa Cruz Biotechnology rabbit polyclonal anti p22 phox
    Nox-4 (a) and <t>p22</t> <t>phox</t> (b) protein expressions in the liver. m/m : Misty; Veh: vehicle-treated db/db mice; K-100: Kangen-karyu 100 mg/kg body weight-treated db/db mice; K-200: Kangen-karyu 200 mg/kg body weight-treated db/db mice. Bars represent the means ± S.E.M. * P < 0.05, ** P < 0.01, *** P < 0.001 versus vehicle-treated db/db mouse values.
    Rabbit Polyclonal Anti P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti p22 phox/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti p22 phox - by Bioz Stars, 2024-03
    95/100 stars
      Buy from Supplier

    95
    Santa Cruz Biotechnology rabbit polyclonal p22 phox
    Expression of NADPH oxidase subunits: (a) gp91 <t>phox</t> , (b) <t>p22</t> phox , (c) NOX4, (d) p47phox, and (e) p67 phox . Group I ( n = 6) was sham-operated animals, which underwent the same surgical procedure without vessel occlusion (control), group II ( n = 6) was subjected to ischemia/reperfusion (I/R), and group III ( n = 5) was treated with vitamin D3 and then subjected to I/R (VitD + I/R). All experiments were performed in synaptosomal fraction in different brain structures. Results are presented as mean ± SE. ∗ p < 0.05 versus control, ∗∗ p < 0.01 versus control, § p < 0.05 versus I/R, and §§ p < 0.01 versus I/R.
    Rabbit Polyclonal P22 Phox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal p22 phox/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal p22 phox - by Bioz Stars, 2024-03
    95/100 stars
      Buy from Supplier

    95
    Santa Cruz Biotechnology polyclonal rabbit anti p22 phox antibody
    Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase state in WT and S100A9 −/− differentiated Hoxb8 cells. ( a ) Expression of the NADPH oxidase subunits. gp91 <t>phox</t> , p67 phox , p47 phox , p40 phox and <t>p22</t> phox expression in non-differentiated (d0) and differentiated (d5) WT or S100A9 −/− Hoxb8 cells was determined by Western Blot. A representative Western Blot of three independent experiments is shown. ( b ) reactive oxygen species (ROS) production stimulated by N-Formylmethionyl-leucyl-phenylalanine (fMLF), phorbolmyristate acetate (PMA) or lipopolysaccharide (LPS). Differentiated WT and S100A9 −/− Hoxb8 cells were stimulated with fMLF (100 nM), PMA (100 nM) or LPS (100 ng/mL). Total ROS production was measured on a plate reader by real-time chemiluminescence. The results represent fold induction of ROS production of S100A9 −/− Hoxb8 cells compared to S100A9 −/− Hoxb8 cells and are expressed as mean ± SEM. Inserts: representative traces showing ROS production in WT Hoxb8 cells stimulated or not with fMLF, PMA or LPS. Significantly different from control * = p < 0.05, ** = p < 0.01.
    Polyclonal Rabbit Anti P22 Phox Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti p22 phox antibody/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti p22 phox antibody - by Bioz Stars, 2024-03
    95/100 stars
      Buy from Supplier

    Image Search Results


    Nox-4 (a) and p22 phox (b) protein expressions in the liver. m/m : Misty; Veh: vehicle-treated db/db mice; K-100: Kangen-karyu 100 mg/kg body weight-treated db/db mice; K-200: Kangen-karyu 200 mg/kg body weight-treated db/db mice. Bars represent the means ± S.E.M. * P < 0.05, ** P < 0.01, *** P < 0.001 versus vehicle-treated db/db mouse values.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Evaluation of Effects of Chinese Prescription Kangen-karyu on Diabetes-Induced Alterations such as Oxidative Stress and Apoptosis in the Liver of Type 2 Diabetic db/db Mice

    doi: 10.1155/2012/143489

    Figure Lengend Snippet: Nox-4 (a) and p22 phox (b) protein expressions in the liver. m/m : Misty; Veh: vehicle-treated db/db mice; K-100: Kangen-karyu 100 mg/kg body weight-treated db/db mice; K-200: Kangen-karyu 200 mg/kg body weight-treated db/db mice. Bars represent the means ± S.E.M. * P < 0.05, ** P < 0.01, *** P < 0.001 versus vehicle-treated db/db mouse values.

    Article Snippet: Rabbit polyclonal antibodies against p22 phox , NF- κ Bp65, nuclear factor erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1), cytochrome c , Bax, and mouse monoclonal antibodies against cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), Bcl-2, and histone were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

    Techniques:

    Expression of NADPH oxidase subunits: (a) gp91 phox , (b) p22 phox , (c) NOX4, (d) p47phox, and (e) p67 phox . Group I ( n = 6) was sham-operated animals, which underwent the same surgical procedure without vessel occlusion (control), group II ( n = 6) was subjected to ischemia/reperfusion (I/R), and group III ( n = 5) was treated with vitamin D3 and then subjected to I/R (VitD + I/R). All experiments were performed in synaptosomal fraction in different brain structures. Results are presented as mean ± SE. ∗ p < 0.05 versus control, ∗∗ p < 0.01 versus control, § p < 0.05 versus I/R, and §§ p < 0.01 versus I/R.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia

    doi: 10.1155/2018/3273654

    Figure Lengend Snippet: Expression of NADPH oxidase subunits: (a) gp91 phox , (b) p22 phox , (c) NOX4, (d) p47phox, and (e) p67 phox . Group I ( n = 6) was sham-operated animals, which underwent the same surgical procedure without vessel occlusion (control), group II ( n = 6) was subjected to ischemia/reperfusion (I/R), and group III ( n = 5) was treated with vitamin D3 and then subjected to I/R (VitD + I/R). All experiments were performed in synaptosomal fraction in different brain structures. Results are presented as mean ± SE. ∗ p < 0.05 versus control, ∗∗ p < 0.01 versus control, § p < 0.05 versus I/R, and §§ p < 0.01 versus I/R.

    Article Snippet: The following primary antibodies were used in this study: mouse polyclonal NeuN (1 : 1000, Millipore, Germany), mouse polyclonal 91- phox (1 : 500, Santa Cruz, CA), rabbit polyclonal p22- phox (1 : 500, Santa Cruz, CA), rabbit polyclonal p47- phox (1 : 500, Santa Cruz, CA), rabbit polyclonal p67- phox (1 : 500, Santa Cruz, CA), rabbit monoclonal NOX4 (1 : 5000, Abcam, UK), rabbit polyclonal VDR (1 : 1000, Abcam, UK), goat polyclonal anti-Iba1 (1 : 250, Abcam, UK), and rabbit polyclonal anti-MMP9 (1 : 1000, Sigma-Aldrich, Germany).

    Techniques: Expressing

    Immunohistochemical staining of gp91 phox and p22 phox in gerbil brain slices. Magnification of images for (a) from A–F is 200×, for (b) from A–C is 40×, and for (b) from D–I is 100×.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia

    doi: 10.1155/2018/3273654

    Figure Lengend Snippet: Immunohistochemical staining of gp91 phox and p22 phox in gerbil brain slices. Magnification of images for (a) from A–F is 200×, for (b) from A–C is 40×, and for (b) from D–I is 100×.

    Article Snippet: The following primary antibodies were used in this study: mouse polyclonal NeuN (1 : 1000, Millipore, Germany), mouse polyclonal 91- phox (1 : 500, Santa Cruz, CA), rabbit polyclonal p22- phox (1 : 500, Santa Cruz, CA), rabbit polyclonal p47- phox (1 : 500, Santa Cruz, CA), rabbit polyclonal p67- phox (1 : 500, Santa Cruz, CA), rabbit monoclonal NOX4 (1 : 5000, Abcam, UK), rabbit polyclonal VDR (1 : 1000, Abcam, UK), goat polyclonal anti-Iba1 (1 : 250, Abcam, UK), and rabbit polyclonal anti-MMP9 (1 : 1000, Sigma-Aldrich, Germany).

    Techniques: Immunohistochemical staining, Staining

    Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase state in WT and S100A9 −/− differentiated Hoxb8 cells. ( a ) Expression of the NADPH oxidase subunits. gp91 phox , p67 phox , p47 phox , p40 phox and p22 phox expression in non-differentiated (d0) and differentiated (d5) WT or S100A9 −/− Hoxb8 cells was determined by Western Blot. A representative Western Blot of three independent experiments is shown. ( b ) reactive oxygen species (ROS) production stimulated by N-Formylmethionyl-leucyl-phenylalanine (fMLF), phorbolmyristate acetate (PMA) or lipopolysaccharide (LPS). Differentiated WT and S100A9 −/− Hoxb8 cells were stimulated with fMLF (100 nM), PMA (100 nM) or LPS (100 ng/mL). Total ROS production was measured on a plate reader by real-time chemiluminescence. The results represent fold induction of ROS production of S100A9 −/− Hoxb8 cells compared to S100A9 −/− Hoxb8 cells and are expressed as mean ± SEM. Inserts: representative traces showing ROS production in WT Hoxb8 cells stimulated or not with fMLF, PMA or LPS. Significantly different from control * = p < 0.05, ** = p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of S100A8/A9 for Cytokine Secretion, Revealed in Neutrophils Derived from ER-Hoxb8 Progenitors

    doi: 10.3390/ijms22168845

    Figure Lengend Snippet: Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase state in WT and S100A9 −/− differentiated Hoxb8 cells. ( a ) Expression of the NADPH oxidase subunits. gp91 phox , p67 phox , p47 phox , p40 phox and p22 phox expression in non-differentiated (d0) and differentiated (d5) WT or S100A9 −/− Hoxb8 cells was determined by Western Blot. A representative Western Blot of three independent experiments is shown. ( b ) reactive oxygen species (ROS) production stimulated by N-Formylmethionyl-leucyl-phenylalanine (fMLF), phorbolmyristate acetate (PMA) or lipopolysaccharide (LPS). Differentiated WT and S100A9 −/− Hoxb8 cells were stimulated with fMLF (100 nM), PMA (100 nM) or LPS (100 ng/mL). Total ROS production was measured on a plate reader by real-time chemiluminescence. The results represent fold induction of ROS production of S100A9 −/− Hoxb8 cells compared to S100A9 −/− Hoxb8 cells and are expressed as mean ± SEM. Inserts: representative traces showing ROS production in WT Hoxb8 cells stimulated or not with fMLF, PMA or LPS. Significantly different from control * = p < 0.05, ** = p < 0.01.

    Article Snippet: NADPH oxidase proteins (p22 phox , p40 phox , p47 phox , p67 phox and gp91 phox ) were detected using the polyclonal rabbit anti- p22 phox antibody (Santa Cruz Biotechnology, Heidelberg, Germany), the monoclonal rabbit anti-p40 phox antibody, clone EP2142Y (Abcam), the polyclonal rabbit anti-mouse p47 phox and anti-mouse p67 phox (Merck-Millipore), and the monoclonal rabbit anti-gp91 phox (clone EPR6991; Abcam).

    Techniques: Expressing, Western Blot