rabbit polyclonal igg  (Valiant)

 
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    Name:
    Anti synaptophysin rabbit polyclonal antibody
    Description:
    Synaptophysin is a calcium binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors
    Catalog Number:
    0810706
    Price:
    698.25
    Category:
    Life Sciences Antibodies Primary Antibodies
    Applications:
    Immunohistochemistry ,Immunoblot
    Size:
    250 µL
    Buy from Supplier


    Structured Review

    Valiant rabbit polyclonal igg
    Synaptophysin is a calcium binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors
    https://www.bioz.com/result/rabbit polyclonal igg/product/Valiant
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal igg - by Bioz Stars, 2021-05
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: A role of stochastic phenotype switching in generating mosaic endothelial cell heterogeneity
    Article Snippet: Immunofluorescent co-staining of en face aorta Aortas were fixed in 4% paraformaldehyde (PFA), washed in PBS, and blocked with TNB Blocking Buffer (TNBT) (0.1 M Tris-HCl, 0.15 M NaCl, 0.2% triton-X-100, 0.5% blocking, Perlin Elmer). .. Aortas were incubated in Image-iT FX signal enhancer (Invitrogen) at room temperature for 30 min and assayed using primary antibodies (rabbit monoclonal vWF (Dako Cat. No. A0082) diluted at 1:1,000, Armenian hamster polyclonal CD31 (Millipore Cat. No. MAB1398Z) diluted at 1:100, and rabbit polyclonal β-galactosidase (MP Biomedicals Cat. No. 08559761) diluted at 1:50) diluted in TNBT at 4 °C overnight. .. Next, aortas were washed with PBS and incubated with secondary antibodies (Alexa Fluor 594 (Invitrogen) and Alexa Fluor 488 (Jackson ImmunoResearch) diluted at 1:200) for 2 h at room temperature.

    Article Title: Neuromuscular Adaptability of Male and Female Rats to Muscle Unloading
    Article Snippet: The next day, sections were washed 4 × 15 min in PBS with 1% BSA before being incubated for 2 h at room temperature in Alexafluor 488 conjugated secondary immunoglobulin (Molecular Probes, Eugene, OR, Cat#A11001; RRID; AB_2534069) which had been diluted 1:200 in PBS with 1% BSA. .. Sections were then washed 4 × 15 min in PBS with 1% BSA before being incubated in a humidified chamber overnight at 4°C in a solution containing rhodamine conjugated α-bungarotoxin (BTX; Molecular Probes, Eugene, OR, Cat# T1175) diluted 1:600 in PBS, along with anti-synaptophysin (MP Biomedicals, Solon, OH, Cat0810706, RRID; AB_2334188) at a dilution of 1:50. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Neuromuscular Adaptability of Male and Female Rats to Muscle Unloading
    Article Snippet: The next day, sections were washed 4 × 15 min in PBS with 1% BSA before being incubated for 2 h at room temperature in Alexafluor 488 conjugated secondary immunoglobulin (Molecular Probes, Eugene, OR, Cat#A11001; RRID; AB_2534069) which had been diluted 1:200 in PBS with 1% BSA. .. Sections were then washed 4 × 15 min in PBS with 1% BSA before being incubated in a humidified chamber overnight at 4°C in a solution containing rhodamine conjugated α-bungarotoxin (BTX; Molecular Probes, Eugene, OR, Cat# T1175) diluted 1:600 in PBS, along with anti-synaptophysin (MP Biomedicals, Solon, OH, Cat0810706, RRID; AB_2334188) at a dilution of 1:50. ..

    Modification:

    Article Title: Activation of Murine Macrophages
    Article Snippet: .. Additional Materials (also see Basic Protocol) OVA: 1 mg/ml chicken egg albumin (OVA; Worthington Biochemical, cat. no. LS003049) in Dulbecco’s modified Eagle medium/F12 (DMEM/F12; GIBCO) Detoxi-gel endotoxin removing column (Pierce, cat. no. 20344) Anti-OVA IgG: 4 mg/ml of rabbit polyclonal IgG to chicken egg albumin (Cappel anti-OVA IgG; MP Biomedicals, cat. no. 55304/05490) in DMEM/F12 Endotoxin removal buffer for protein solution (EndoClean; BioVintage, cat. no. 18602) Mini-rotator (Glas-Col), or equivalent Additional reagents and equipment for performing ELISA ( unit 2.1 ) Remove endotoxin from OVA with a Detoxi-Gel endotoxin removing column, following manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Activation of Murine Macrophages
    Article Snippet: .. Additional Materials (also see Basic Protocol) OVA: 1 mg/ml chicken egg albumin (OVA; Worthington Biochemical, cat. no. LS003049) in Dulbecco’s modified Eagle medium/F12 (DMEM/F12; GIBCO) Detoxi-gel endotoxin removing column (Pierce, cat. no. 20344) Anti-OVA IgG: 4 mg/ml of rabbit polyclonal IgG to chicken egg albumin (Cappel anti-OVA IgG; MP Biomedicals, cat. no. 55304/05490) in DMEM/F12 Endotoxin removal buffer for protein solution (EndoClean; BioVintage, cat. no. 18602) Mini-rotator (Glas-Col), or equivalent Additional reagents and equipment for performing ELISA ( unit 2.1 ) Remove endotoxin from OVA with a Detoxi-Gel endotoxin removing column, following manufacturer’s instructions. ..

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  • 92
    Valiant rabbit anti β galactosidase
    Evidence for EMT associated with intestinal fibrosis in Villin-LacZ mice. Frozen colonic tissue sections from ethanol control and TNBS-treated Villin-LacZ mice were either labeled with antibodies to <t>β-gal</t> ( red ) and FSP1 ( green ) or with antibodies
    Rabbit Anti β Galactosidase, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti β galactosidase/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti β galactosidase - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    86
    Valiant goat igg polyclonal antibodies conjugated
    Evidence for EMT associated with intestinal fibrosis in Villin-LacZ mice. Frozen colonic tissue sections from ethanol control and TNBS-treated Villin-LacZ mice were either labeled with antibodies to <t>β-gal</t> ( red ) and FSP1 ( green ) or with antibodies
    Goat Igg Polyclonal Antibodies Conjugated, supplied by Valiant, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat igg polyclonal antibodies conjugated/product/Valiant
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat igg polyclonal antibodies conjugated - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Evidence for EMT associated with intestinal fibrosis in Villin-LacZ mice. Frozen colonic tissue sections from ethanol control and TNBS-treated Villin-LacZ mice were either labeled with antibodies to β-gal ( red ) and FSP1 ( green ) or with antibodies

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of Epithelial to Mesenchymal Transition as a Novel Source of Fibroblasts in Intestinal Fibrosis *

    doi: 10.1074/jbc.M110.102012

    Figure Lengend Snippet: Evidence for EMT associated with intestinal fibrosis in Villin-LacZ mice. Frozen colonic tissue sections from ethanol control and TNBS-treated Villin-LacZ mice were either labeled with antibodies to β-gal ( red ) and FSP1 ( green ) or with antibodies

    Article Snippet: The sections were incubated with the following primary antibodies at 4 °C overnight depending on the experiment: mouse anti-E-cadherin (BD Bioscience), rabbit anti-FSP1 (polyclonal antibody; research gift from Eric Neilson, Vanderbilt University, Nashville, TN), fluorescein isothiocyanate-conjugated mouse anti-α-SMA (Sigma), or rabbit anti-β-galactosidase (β-gal; polyclonal antibody; Cappel, MP Biomedicals).

    Techniques: Mouse Assay, Labeling

    Immunodetection of pendrin and/or β-galactosidase. In cryostat sections from adult cochleas, pendrin (yellow) appears in spiral prominence (Sp) and root cells (Rc) with a similar pattern of expression in Kcnk5 +/ − ( a ) and Kcnk5 −/− ( b ) mice at P45. DAPI (4′,6-diamidino-2-phenylindole dihydrochoride) staining of cell nuclei (blue). In adult Kcnk5 +/ − mouse cochlea ( c – k ), β-galactosidase (green) mainly found in Claudius' cells (Cc, c , d , i , j ), is also present in Rc ( e , k ), while pendrin (magenta) is localized in Rc ( c – h ). Merged images ( c – e ) show that pendrin and β-galactosidase only colocalize in Rc. Stacks of 23 confocal planes of 1.8 μm each in c , f and i , single confocal planes of 1.8 μm optic thickness in d , g and j and single confocal planes of 0.7 μm optic thickness in e , h and k . Scale bars, 50 μm ( a – c , f , i ); 20 μm ( d , e , g , h , j , k ).

    Journal: Nature Communications

    Article Title: KCNK5 channels mostly expressed in cochlear outer sulcus cells are indispensable for hearing

    doi: 10.1038/ncomms9780

    Figure Lengend Snippet: Immunodetection of pendrin and/or β-galactosidase. In cryostat sections from adult cochleas, pendrin (yellow) appears in spiral prominence (Sp) and root cells (Rc) with a similar pattern of expression in Kcnk5 +/ − ( a ) and Kcnk5 −/− ( b ) mice at P45. DAPI (4′,6-diamidino-2-phenylindole dihydrochoride) staining of cell nuclei (blue). In adult Kcnk5 +/ − mouse cochlea ( c – k ), β-galactosidase (green) mainly found in Claudius' cells (Cc, c , d , i , j ), is also present in Rc ( e , k ), while pendrin (magenta) is localized in Rc ( c – h ). Merged images ( c – e ) show that pendrin and β-galactosidase only colocalize in Rc. Stacks of 23 confocal planes of 1.8 μm each in c , f and i , single confocal planes of 1.8 μm optic thickness in d , g and j and single confocal planes of 0.7 μm optic thickness in e , h and k . Scale bars, 50 μm ( a – c , f , i ); 20 μm ( d , e , g , h , j , k ).

    Article Snippet: Air-dried cryostat sections (see above) from 4% paraformaldehyde-fixed whole cochleas were rehydrated in PBS, kept in a blocking solution (0.1% Triton-X 100 with 1% bovine serum albumin and 1% normal goat serum in PBS) for 30 min at room temperature and then incubated in primary antibody in the blocking solution, overnight at 4 °C—the rabbit monoclonal anti-β-galactosidase (cat. # 55976, Cappel, MP Biomedicals, Solon, OH) was used at 1:500; the rabbit polyclonal anti-pendrin (generous gift from Dr R. Chambrey; an antibody initially characterized by Royaux et al . ) at 1:500; the rabbit polyclonal anti-KCNJ10 (cat. # APC-035, Alomone Lab Ltd, Jerusalem, Israel) at 1:400 or the rabbit monoclonal anti-KCNJ10 (cat. # ab80959, Abcam, Cambridge, UK) at 1:100; the polyclonal rabbit anti-KCNQ1 (cat. # APC-022, Alomone Lab Ltd) at 1:100; and the mouse monoclonal anti-acetylated tubulin (cat. # T7451, Sigma-Aldrich) at 1:1,000.

    Techniques: Immunodetection, Expressing, Mouse Assay, Staining

    Electroporation of the expression vectors with astrocyte-specific promoter. ( a–e ) The electroporation of pGLAST-DsRed, in which DsRed was driven by rat GLAST promoter. GFP expression vector with CAG promoter was co-transfected with pGLAST-DsRed. After the focal electroporation, DsRed expression is detected in GLAST-immunoreactive cells. ( f–j ) The electroporation of pGFAP-LacZ in which ß-galactosidase was driven by mouse GFAP promoter. GFP expression vector with CAG promoter was co-transfected with pGFAP-LacZ. After the focal electroporation, LacZ expression was detected in GFAP-immunoreactive cells. ( k–m ) Doxycycline-dependent gene expression in astrocytic cells. The adult SVZ was co-transfected with pGFAP-TetON and the reporter plasmid (pTRE-ZsGreen) via focal electroporation ( k ). ZsGreen expression in an astrocytic cell (a yellow arrow) but not in an ependymal cell (a red arrow) after doxycycline administration ( l,m ). Scale bar: 25 μm in ( b ).

    Journal: Scientific Reports

    Article Title: Rapid and efficient gene delivery into the adult mouse brain via focal electroporation

    doi: 10.1038/srep29817

    Figure Lengend Snippet: Electroporation of the expression vectors with astrocyte-specific promoter. ( a–e ) The electroporation of pGLAST-DsRed, in which DsRed was driven by rat GLAST promoter. GFP expression vector with CAG promoter was co-transfected with pGLAST-DsRed. After the focal electroporation, DsRed expression is detected in GLAST-immunoreactive cells. ( f–j ) The electroporation of pGFAP-LacZ in which ß-galactosidase was driven by mouse GFAP promoter. GFP expression vector with CAG promoter was co-transfected with pGFAP-LacZ. After the focal electroporation, LacZ expression was detected in GFAP-immunoreactive cells. ( k–m ) Doxycycline-dependent gene expression in astrocytic cells. The adult SVZ was co-transfected with pGFAP-TetON and the reporter plasmid (pTRE-ZsGreen) via focal electroporation ( k ). ZsGreen expression in an astrocytic cell (a yellow arrow) but not in an ependymal cell (a red arrow) after doxycycline administration ( l,m ). Scale bar: 25 μm in ( b ).

    Article Snippet: The frozen brains were sectioned at a thickness of 35 μm using a Cryostat (CM1900 and CM1850, Leica), and incubated with primary antibodies, including anti-GFP (rabbit polyclonal, Life Technology or rat monoclonal, Nakarai Tesque), anti-RFP (rabbit polyclonal, Abcam), anti-GLAST (goat polyclonal, Frontier Institute), anti-GFAP (mouse monoclonal, SIGMA), anti-ß-gal (rabbit polyclonal, MP Biomedicals), anti-activated caspase3 (rabbit polyclonal, Cell signaling), and anti-S100 (rabbit polyclonal, DAKO).

    Techniques: Electroporation, Expressing, Plasmid Preparation, Transfection