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Abcam rabbit polyclonal egfp antibody ab
Rabbit Polyclonal Egfp Antibody Ab, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal egfp antibody ab/product/Abcam
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal egfp antibody ab - by Bioz Stars, 2020-09
88/100 stars

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Article Title: A novel dopamine transporter transgenic mouse line for identification and purification of midbrain dopaminergic neurons reveals midbrain heterogeneity
Article Snippet: .. Sections were incubated with rabbit polyclonal eGFP antibody (AB) (1:1000, Abcam, USA) at 4°C overnight. .. On the second day, sections were incubated with biotinylated goat anti-rabbit IgG for 1h (1:400, DAKO Cytomation A/S, Denmark).

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  • egfp  (Abcam)
    93
    Abcam egfp
    Early colonization of PLNs by i.f.-inoculated <t>MuHV-4.</t> (a) C57BL/6 mice were infected i.f. with MHV-GFP. Sections of PLNs harvested 3 and 5 days later were stained for virus-expressed <t>eGFP</t> (green in merge) and for cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of at least six mice per time point. Arrowheads show examples of green/red co-localization. (b) eGFP + cells were counted for five mice (three sections per mouse). Bars show means ± sem for eGFP + cells expressing each marker (co-localization with ER-TR7 was
    Egfp, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfp/product/Abcam
    Average 93 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    egfp - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Abcam anti gfp antibody
    TRIM21 targets Lys-190 in <t>SALL4</t> amino acid sequence. A , structures of fragmented-SALL4B-EGFP probes are shown. NLS , nuclear localization signal. B , EGFP signals of MCF-7 cells expressing the probes are shown ( n = 3). TRIM21 knockdown was performed with shTRIM21-5. The identified TRIM21 target region (aa 172–255) is depicted. C , the immunoblot image shows <t>GFP</t> immunoreactions of MCF-7 cells expressing SALL4B(1–255)-EGFP probes with Lys to Arg mutations ( mut. ) at residues 173, 175, and 190 ( n = 3). D , TRIM21 knockdown was performed in MCF-7 cells expressing the SALL4B(1–255) K190R-EGFP probe ( n = 3). E , the immunoblot image shows the signal of the polyubiquitinated SALL4B(1–255)-EGFP probe ( n = 3). MCF-7 cells were treated with MG-132. Input samples were used for the total amount of each probe (α-GFP) and internal control (α-β-actin). F , the immunoblot image shows overexpression of the SALL4B-EGFP probe with or without K190R mutation ( n = 3). Short- and long-exposure images of GFP immunoreaction are shown. The results of migration assays are shown. Arrowheads indicate migrated cells. Scale bar , 100 μm. The graph shows the migrated cell numbers. n.s. , not significant; *, p
    Anti Gfp Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gfp antibody/product/Abcam
    Average 99 stars, based on 494 article reviews
    Price from $9.99 to $1999.99
    anti gfp antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Abcam rabbit polyclonal anti egfp antibodies
    TRIM21 targets Lys-190 in <t>SALL4</t> amino acid sequence. A , structures of fragmented-SALL4B-EGFP probes are shown. NLS , nuclear localization signal. B , EGFP signals of MCF-7 cells expressing the probes are shown ( n = 3). TRIM21 knockdown was performed with shTRIM21-5. The identified TRIM21 target region (aa 172–255) is depicted. C , the immunoblot image shows <t>GFP</t> immunoreactions of MCF-7 cells expressing SALL4B(1–255)-EGFP probes with Lys to Arg mutations ( mut. ) at residues 173, 175, and 190 ( n = 3). D , TRIM21 knockdown was performed in MCF-7 cells expressing the SALL4B(1–255) K190R-EGFP probe ( n = 3). E , the immunoblot image shows the signal of the polyubiquitinated SALL4B(1–255)-EGFP probe ( n = 3). MCF-7 cells were treated with MG-132. Input samples were used for the total amount of each probe (α-GFP) and internal control (α-β-actin). F , the immunoblot image shows overexpression of the SALL4B-EGFP probe with or without K190R mutation ( n = 3). Short- and long-exposure images of GFP immunoreaction are shown. The results of migration assays are shown. Arrowheads indicate migrated cells. Scale bar , 100 μm. The graph shows the migrated cell numbers. n.s. , not significant; *, p
    Rabbit Polyclonal Anti Egfp Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti egfp antibodies/product/Abcam
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti egfp antibodies - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Early colonization of PLNs by i.f.-inoculated MuHV-4. (a) C57BL/6 mice were infected i.f. with MHV-GFP. Sections of PLNs harvested 3 and 5 days later were stained for virus-expressed eGFP (green in merge) and for cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of at least six mice per time point. Arrowheads show examples of green/red co-localization. (b) eGFP + cells were counted for five mice (three sections per mouse). Bars show means ± sem for eGFP + cells expressing each marker (co-localization with ER-TR7 was

    Journal: The Journal of General Virology

    Article Title: Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination

    doi: 10.1099/vir.0.000140

    Figure Lengend Snippet: Early colonization of PLNs by i.f.-inoculated MuHV-4. (a) C57BL/6 mice were infected i.f. with MHV-GFP. Sections of PLNs harvested 3 and 5 days later were stained for virus-expressed eGFP (green in merge) and for cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of at least six mice per time point. Arrowheads show examples of green/red co-localization. (b) eGFP + cells were counted for five mice (three sections per mouse). Bars show means ± sem for eGFP + cells expressing each marker (co-localization with ER-TR7 was

    Article Snippet: Sections (6 μm) were air dried (1 h, 23 °C), blocked with 0.3 % Triton X-100, 5 % normal goat serum (1 h, 23 °C) and then incubated (18 h, 4 °C) with combinations of primary antibodies to eGFP [rabbit polyclonal Ab (pAb); Abcam], MuHV-4 (rabbit pAb, raised in house against whole virus), CD169 (rat mAb 3D6.112; Serotec), CD45RB/B220 (rat mAb RA3-6B2; Abcam), CD68 (rat mAb FA-11; Biolegend), ER-TR7 (rat mAb; AbCam) and CD11c (hamster mAb N418).

    Techniques: Mouse Assay, Infection, Staining, Expressing, Marker

    MuHV-4 spread to the spleen in mice depleted of SSMs. (a) C57BL/6 mice were depleted or not of SSMs by i.f. clodronate-loaded liposomes, 3 and 5 days before i.f. inoculation of replication-deficient (ORF50 − ) MHV-GFP (10 5 p.f.u.). Four days later, PLN sections were stained for viral eGFP (green in merge) and CD169 (red). Nuclei were stained with DAPI (blue). Arrowheads show example CD169 + cells, which were essentially absent after clodronate treatment. (b) Mean ± sem counts of eGFP + cells from three sections each of three mice per group showed a significant increase with clodronate (* P

    Journal: The Journal of General Virology

    Article Title: Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination

    doi: 10.1099/vir.0.000140

    Figure Lengend Snippet: MuHV-4 spread to the spleen in mice depleted of SSMs. (a) C57BL/6 mice were depleted or not of SSMs by i.f. clodronate-loaded liposomes, 3 and 5 days before i.f. inoculation of replication-deficient (ORF50 − ) MHV-GFP (10 5 p.f.u.). Four days later, PLN sections were stained for viral eGFP (green in merge) and CD169 (red). Nuclei were stained with DAPI (blue). Arrowheads show example CD169 + cells, which were essentially absent after clodronate treatment. (b) Mean ± sem counts of eGFP + cells from three sections each of three mice per group showed a significant increase with clodronate (* P

    Article Snippet: Sections (6 μm) were air dried (1 h, 23 °C), blocked with 0.3 % Triton X-100, 5 % normal goat serum (1 h, 23 °C) and then incubated (18 h, 4 °C) with combinations of primary antibodies to eGFP [rabbit polyclonal Ab (pAb); Abcam], MuHV-4 (rabbit pAb, raised in house against whole virus), CD169 (rat mAb 3D6.112; Serotec), CD45RB/B220 (rat mAb RA3-6B2; Abcam), CD68 (rat mAb FA-11; Biolegend), ER-TR7 (rat mAb; AbCam) and CD11c (hamster mAb N418).

    Techniques: Mouse Assay, Staining

    Early SCLN infection by i.n. MuHV-4 inoculation. (a) C57BL/6 mice were infected i.n. with MHV-GFP (10 5 p.f.u.). Sections of SCLNs harvested 5 and 7 days later were stained for viral eGFP (green in merge) and cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of six mice per time point. Arrows show examples of green/red co-localization. Scale bar = 10 μm. (b) eGFP + cells were counted for five mice (three sections per mouse). Bars show mean ± sem for eGFP + cells expressing each marker (co-localization with ER-TR7 was

    Journal: The Journal of General Virology

    Article Title: Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination

    doi: 10.1099/vir.0.000140

    Figure Lengend Snippet: Early SCLN infection by i.n. MuHV-4 inoculation. (a) C57BL/6 mice were infected i.n. with MHV-GFP (10 5 p.f.u.). Sections of SCLNs harvested 5 and 7 days later were stained for viral eGFP (green in merge) and cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of six mice per time point. Arrows show examples of green/red co-localization. Scale bar = 10 μm. (b) eGFP + cells were counted for five mice (three sections per mouse). Bars show mean ± sem for eGFP + cells expressing each marker (co-localization with ER-TR7 was

    Article Snippet: Sections (6 μm) were air dried (1 h, 23 °C), blocked with 0.3 % Triton X-100, 5 % normal goat serum (1 h, 23 °C) and then incubated (18 h, 4 °C) with combinations of primary antibodies to eGFP [rabbit polyclonal Ab (pAb); Abcam], MuHV-4 (rabbit pAb, raised in house against whole virus), CD169 (rat mAb 3D6.112; Serotec), CD45RB/B220 (rat mAb RA3-6B2; Abcam), CD68 (rat mAb FA-11; Biolegend), ER-TR7 (rat mAb; AbCam) and CD11c (hamster mAb N418).

    Techniques: Infection, Mouse Assay, Staining, Expressing, Marker

    PLN infection by i.f. replication-deficient MuHV-4. (a) C57BL/6 mice were infected i.f. with replication-deficient MHV-GFP (ORF50 − ). Sections of PLN harvested 3 and 5 days later were stained for viral eGFP (green in merge) and cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of six mice per time point. Arrows show examples of green/red co-localization. Scale bar = 10 μm. (b) EGFP + cells were counted for five mice (three sections per mouse). Bars show mean ± sem for eGFP + cells expressing each marker (co-localization with ER-TR7 was

    Journal: The Journal of General Virology

    Article Title: Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination

    doi: 10.1099/vir.0.000140

    Figure Lengend Snippet: PLN infection by i.f. replication-deficient MuHV-4. (a) C57BL/6 mice were infected i.f. with replication-deficient MHV-GFP (ORF50 − ). Sections of PLN harvested 3 and 5 days later were stained for viral eGFP (green in merge) and cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of six mice per time point. Arrows show examples of green/red co-localization. Scale bar = 10 μm. (b) EGFP + cells were counted for five mice (three sections per mouse). Bars show mean ± sem for eGFP + cells expressing each marker (co-localization with ER-TR7 was

    Article Snippet: Sections (6 μm) were air dried (1 h, 23 °C), blocked with 0.3 % Triton X-100, 5 % normal goat serum (1 h, 23 °C) and then incubated (18 h, 4 °C) with combinations of primary antibodies to eGFP [rabbit polyclonal Ab (pAb); Abcam], MuHV-4 (rabbit pAb, raised in house against whole virus), CD169 (rat mAb 3D6.112; Serotec), CD45RB/B220 (rat mAb RA3-6B2; Abcam), CD68 (rat mAb FA-11; Biolegend), ER-TR7 (rat mAb; AbCam) and CD11c (hamster mAb N418).

    Techniques: Infection, Mouse Assay, Staining, Expressing, Marker

    TRIM21 targets Lys-190 in SALL4 amino acid sequence. A , structures of fragmented-SALL4B-EGFP probes are shown. NLS , nuclear localization signal. B , EGFP signals of MCF-7 cells expressing the probes are shown ( n = 3). TRIM21 knockdown was performed with shTRIM21-5. The identified TRIM21 target region (aa 172–255) is depicted. C , the immunoblot image shows GFP immunoreactions of MCF-7 cells expressing SALL4B(1–255)-EGFP probes with Lys to Arg mutations ( mut. ) at residues 173, 175, and 190 ( n = 3). D , TRIM21 knockdown was performed in MCF-7 cells expressing the SALL4B(1–255) K190R-EGFP probe ( n = 3). E , the immunoblot image shows the signal of the polyubiquitinated SALL4B(1–255)-EGFP probe ( n = 3). MCF-7 cells were treated with MG-132. Input samples were used for the total amount of each probe (α-GFP) and internal control (α-β-actin). F , the immunoblot image shows overexpression of the SALL4B-EGFP probe with or without K190R mutation ( n = 3). Short- and long-exposure images of GFP immunoreaction are shown. The results of migration assays are shown. Arrowheads indicate migrated cells. Scale bar , 100 μm. The graph shows the migrated cell numbers. n.s. , not significant; *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Sal-like 4 protein levels in breast cancer cells are post-translationally down-regulated by tripartite motif–containing 21

    doi: 10.1074/jbc.RA117.000245

    Figure Lengend Snippet: TRIM21 targets Lys-190 in SALL4 amino acid sequence. A , structures of fragmented-SALL4B-EGFP probes are shown. NLS , nuclear localization signal. B , EGFP signals of MCF-7 cells expressing the probes are shown ( n = 3). TRIM21 knockdown was performed with shTRIM21-5. The identified TRIM21 target region (aa 172–255) is depicted. C , the immunoblot image shows GFP immunoreactions of MCF-7 cells expressing SALL4B(1–255)-EGFP probes with Lys to Arg mutations ( mut. ) at residues 173, 175, and 190 ( n = 3). D , TRIM21 knockdown was performed in MCF-7 cells expressing the SALL4B(1–255) K190R-EGFP probe ( n = 3). E , the immunoblot image shows the signal of the polyubiquitinated SALL4B(1–255)-EGFP probe ( n = 3). MCF-7 cells were treated with MG-132. Input samples were used for the total amount of each probe (α-GFP) and internal control (α-β-actin). F , the immunoblot image shows overexpression of the SALL4B-EGFP probe with or without K190R mutation ( n = 3). Short- and long-exposure images of GFP immunoreaction are shown. The results of migration assays are shown. Arrowheads indicate migrated cells. Scale bar , 100 μm. The graph shows the migrated cell numbers. n.s. , not significant; *, p

    Article Snippet: The primary antibodies were anti-SALL4 antibody (ab29112, lot number GR146101-1, Abcam; 1:1000 dilution), anti-GFP antibody (ab290, lot number GR19413-1, Abcam; 1:1000 dilution; validated by immunoblotting in EGFP-overexpressing cells), anti-SALL1 antibody (AP17204a, lot number SA111125AA, Abgent, San Diego, CA; 1:1000 dilution; validated by immunoblotting in TRIM21 knockdown cells), and anti-β-actin antibody (ab6276, lot number 66278-2, Abcam; 1:5000 dilution).

    Techniques: Sequencing, Expressing, Over Expression, Mutagenesis, Migration

    Biodistribution of scAAVHSC-CBA-eGFP in the brain and peripheral organs of nonhuman primates. (A) Levels of vector genomes in the brain of animals treated with an IV dose of scAAVHSC17-CBA-eGFP (black bars), scAAVHSC15-CBA-eGFP (blue bars) or scAAVHSC7-CBA-eGFP (grey bars). (B) Levels of vector genomes within peripheral tissues of animals treated with an IV dose of scAAVHSC17-CBA-eGFP (black bars), scAAVHSC15-CBA-eGFP (blue bars) or scAAVHSC7-CBA-eGFP (grey bars). Two weeks after dosing tissues were harvested and processed for GFP vector genome analyses as described under Materials and methods. The bars in panel A represent the mean ± SD of n = 2–12 pieces of tissue from each brain area of each animal with each assay performed in triplicate. The background level of eGFP vector genomes/cell measured across all brain areas in animals treated with vehicle was 0.009. The bars in panel B represent the mean ± SD of n = 6 tissue sections from each animal treated with scAAVHSC15-CBA-eGFP or scAAVHSC17-CBA-eGFP and n = 3 tissue sections from the animal treated with scAAVHSC7-CBA-eGFP. Eye samples were tissues sections taken through an intact fixed eye from each animal and retinal images are shown. Olfactory bulb samples were only collected from the animals treated with scAAVHSC17-CBA-eGFP and biceps, diaphragm, and esophageal tissues were only collected from animals treated with scAAVHSC15-CBA-eGFP and scAAVHSC7-CBA-eGFP. MLN = mesenteric lymph node, PLN = peripheral lymph node.

    Journal: PLoS ONE

    Article Title: Clade F AAVHSCs cross the blood brain barrier and transduce the central nervous system in addition to peripheral tissues following intravenous administration in nonhuman primates

    doi: 10.1371/journal.pone.0225582

    Figure Lengend Snippet: Biodistribution of scAAVHSC-CBA-eGFP in the brain and peripheral organs of nonhuman primates. (A) Levels of vector genomes in the brain of animals treated with an IV dose of scAAVHSC17-CBA-eGFP (black bars), scAAVHSC15-CBA-eGFP (blue bars) or scAAVHSC7-CBA-eGFP (grey bars). (B) Levels of vector genomes within peripheral tissues of animals treated with an IV dose of scAAVHSC17-CBA-eGFP (black bars), scAAVHSC15-CBA-eGFP (blue bars) or scAAVHSC7-CBA-eGFP (grey bars). Two weeks after dosing tissues were harvested and processed for GFP vector genome analyses as described under Materials and methods. The bars in panel A represent the mean ± SD of n = 2–12 pieces of tissue from each brain area of each animal with each assay performed in triplicate. The background level of eGFP vector genomes/cell measured across all brain areas in animals treated with vehicle was 0.009. The bars in panel B represent the mean ± SD of n = 6 tissue sections from each animal treated with scAAVHSC15-CBA-eGFP or scAAVHSC17-CBA-eGFP and n = 3 tissue sections from the animal treated with scAAVHSC7-CBA-eGFP. Eye samples were tissues sections taken through an intact fixed eye from each animal and retinal images are shown. Olfactory bulb samples were only collected from the animals treated with scAAVHSC17-CBA-eGFP and biceps, diaphragm, and esophageal tissues were only collected from animals treated with scAAVHSC15-CBA-eGFP and scAAVHSC7-CBA-eGFP. MLN = mesenteric lymph node, PLN = peripheral lymph node.

    Article Snippet: Histology and microscopy Non-CNS paraffin-embedded tissues (except dorsal root ganglia) were sectioned at 4–5 microns and were processed for eGFP immunohistochemistry (IHC) at Premier Laboratory (Boulder, CO). eGFP-positive cells were stained by IHC with a rabbit anti-eGFP polyclonal antibody (Abcam #ab290).

    Techniques: Crocin Bleaching Assay, Plasmid Preparation