rabbit polyclonal antiserum against mers cov  (Sino Biological)


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    Name:
    MERS CoV Spike Protein Antibody Rabbit PAb
    Description:
    Produced in rabbits immunized with purified recombinant MERS CoV NCoV Novel coronavirus Spike Protein ECD aa 1 1297 Catalog 40069 V08B AFS88936 1 Met1 Trp1297 MERS CoV NCoV Novel coronavirus Spike Protein ECD aa 1 1297 specific IgG was purified by MERS CoV NCoV Novel coronavirus Spike Protein ECD aa 1 1297 affinity chromatography
    Catalog Number:
    40069-T30
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    MERS CoV
    Applications:
    ELISA
    Immunogen:
    Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297) Protein (Catalog#40069-V08B)
    Product Aliases:
    Anti-coronavirus s1 Antibody, Anti-coronavirus s2 Antibody, Anti-coronavirus spike Antibody, Anti-cov spike Antibody, Anti-ncov RBD Antibody, Anti-ncov s1 Antibody, Anti-ncov s2 Antibody, Anti-ncov spike Antibody, Anti-RBD Antibody, Anti-S Antibody, Anti-s1 Antibody, Anti-Spike RBD Antibody
    Antibody Type:
    PAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological rabbit polyclonal antiserum against mers cov
    Sequencing of <t>MERS-CoV</t> Spike protein and data analysis
    Produced in rabbits immunized with purified recombinant MERS CoV NCoV Novel coronavirus Spike Protein ECD aa 1 1297 Catalog 40069 V08B AFS88936 1 Met1 Trp1297 MERS CoV NCoV Novel coronavirus Spike Protein ECD aa 1 1297 specific IgG was purified by MERS CoV NCoV Novel coronavirus Spike Protein ECD aa 1 1297 affinity chromatography
    https://www.bioz.com/result/rabbit polyclonal antiserum against mers cov/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antiserum against mers cov - by Bioz Stars, 2021-05
    95/100 stars

    Images

    1) Product Images from "Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease"

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    Journal: Virology

    doi: 10.1016/j.virol.2015.07.013

    Sequencing of MERS-CoV Spike protein and data analysis
    Figure Legend Snippet: Sequencing of MERS-CoV Spike protein and data analysis

    Techniques Used: Sequencing

    Gross and Histopathology. (A) Gross lung pathology demonstrating mostly normal lung with multifocal to coalescing moderate interstitial pneumonia in the left caudal lung lobe. (B) Low magnification of lung field from MERS-CoV EMC inoculated subject demonstrating
    Figure Legend Snippet: Gross and Histopathology. (A) Gross lung pathology demonstrating mostly normal lung with multifocal to coalescing moderate interstitial pneumonia in the left caudal lung lobe. (B) Low magnification of lung field from MERS-CoV EMC inoculated subject demonstrating

    Techniques Used: Histopathology

    Ex-vivo analysis of primary cells. Cells were isolated from lung, kidney, and bronchoalveolar lavage (BAL), and one-step growth kinetics were performed as described in Materials and Methods. Lung, Kidney and BAL demonstrate that MERS-CoV is able to replicate
    Figure Legend Snippet: Ex-vivo analysis of primary cells. Cells were isolated from lung, kidney, and bronchoalveolar lavage (BAL), and one-step growth kinetics were performed as described in Materials and Methods. Lung, Kidney and BAL demonstrate that MERS-CoV is able to replicate

    Techniques Used: Ex Vivo, Isolation

    Fluorescence Reduction Neutralizing Assay. Sera from all subjects was evaluated for the presence of neutralizing antibody to MERS-CoV as described in Materials and Methods. Neutralizing antibody could be detected above the background of the Mock infected
    Figure Legend Snippet: Fluorescence Reduction Neutralizing Assay. Sera from all subjects was evaluated for the presence of neutralizing antibody to MERS-CoV as described in Materials and Methods. Neutralizing antibody could be detected above the background of the Mock infected

    Techniques Used: Fluorescence, Neutralizing Assay, Infection

    Related Articles

    Staining:

    Article Title: Structural Definition of a Neutralization-Sensitive Epitope on the MERS-CoV S1-NTD
    Article Snippet: After incubation, cells were washed, fixed with 80% cold acetone, and rewashed. .. Subsequently, cells were stained with MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) and then secondary goat anti-rabbit IgG H & L labeled with Alexa Fluor® 488 (AF488) was added. .. Finally, cell nuclei were stained with DAPI.

    Labeling:

    Article Title: Structural Definition of a Neutralization-Sensitive Epitope on the MERS-CoV S1-NTD
    Article Snippet: After incubation, cells were washed, fixed with 80% cold acetone, and rewashed. .. Subsequently, cells were stained with MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) and then secondary goat anti-rabbit IgG H & L labeled with Alexa Fluor® 488 (AF488) was added. .. Finally, cell nuclei were stained with DAPI.

    Incubation:

    Article Title: Characterization of a human monoclonal antibody generated from a B-cell specific for a prefusion-stabilized spike protein of Middle East respiratory syndrome coronavirus
    Article Snippet: Then, human anti-MERS-CoV antibodies were incubated with the Vero cells infected with MERS-CoV. .. As positive controls, a rabbit polyclonal anti-MERS-CoV spike protein antibody (Sino biological Inc., Beijing, China) for MERS-CoV, a mouse anti-229E coronavirus nucleoprotein OC-43 antibody (MERCK, Darmstadt, Germany) for HCoV-229E, a mouse anti-coronavirus antibody, hCoV OC-43 (LifeSpan BioScience, Seattle, WA, USA) for HCoV-OC43, a rabbit polyclonal anti-hCoV-HKU1 spike protein antibody (Sino Biological Inc., Beijing, China) for hCoV-NL63, a human serum of a Korean SARS-CoV-2 convalescent person were also incubated. .. The secondary antibodies, Fluorescein isothiocyanate (FITC) -conjugated goat anti-human-IgG (Abcam, Cambridge, U.K.), FITC-conjugated goat anti-rabbit-IgG (Abcam, Cambridge, U.K.), and FITC-conjugated goat anti-mouse-IgG (Jackson Immunoresearch, West Grove, PA, USA) were added to the cells and incubated.

    Article Title: A Novel Bacterium-Like Particle Vaccine Displaying the MERS-CoV Receptor-Binding Domain Induces Specific Mucosal and Systemic Immune Responses in Mice
    Article Snippet: Briefly, Sf9 cells cultured in 96-well plates at 2 × 106 cells/mL were infected with the recombinant baculovirus. .. After 48 h of infection, the cultured plates were fixed with 80% cold acetone overnight at -20°C, washed three times with PBS-0.05% Tween 20 (PBST), and then incubated with a rabbit anti-MERS-S polyclonal antibody (1:500, Sino Biological Inc, Beijing, China) containing 1% bovine serum albumin (BSA, Sigma-Aldrich, USA) at 37°C for 1 h. After three washes with PBST, an FITC-labeled goat against rabbit IgG antibody (1:300, BioWorld, Inc, St. Louis, MN, USA) was added with Evans blue (Sigma-Aldrich, St. Louis, MN, USA) for 1 h at 37 °C. ..

    Article Title: A high-throughput inhibition assay to study MERS-CoV antibody interactions using image cytometry
    Article Snippet: After incubation, cells were washed with PBS, fixed with 80% cold acetone for 20 min at RT, and washed again with PBS. .. Subsequently, 100 μL of MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) was pipetted into each well, incubated for 60 min at RT, and washed twice. .. Next, secondary goat anti-rabbit IgG H & L labeled with Alexa Fluor® 488 (AF488) and DPP4 PE-conjugated mouse antibody (Sino Biological) were added, incubated for 60 min at RT, and washed twice.

    Infection:

    Article Title: A Novel Bacterium-Like Particle Vaccine Displaying the MERS-CoV Receptor-Binding Domain Induces Specific Mucosal and Systemic Immune Responses in Mice
    Article Snippet: Briefly, Sf9 cells cultured in 96-well plates at 2 × 106 cells/mL were infected with the recombinant baculovirus. .. After 48 h of infection, the cultured plates were fixed with 80% cold acetone overnight at -20°C, washed three times with PBS-0.05% Tween 20 (PBST), and then incubated with a rabbit anti-MERS-S polyclonal antibody (1:500, Sino Biological Inc, Beijing, China) containing 1% bovine serum albumin (BSA, Sigma-Aldrich, USA) at 37°C for 1 h. After three washes with PBST, an FITC-labeled goat against rabbit IgG antibody (1:300, BioWorld, Inc, St. Louis, MN, USA) was added with Evans blue (Sigma-Aldrich, St. Louis, MN, USA) for 1 h at 37 °C. ..

    Cell Culture:

    Article Title: A Novel Bacterium-Like Particle Vaccine Displaying the MERS-CoV Receptor-Binding Domain Induces Specific Mucosal and Systemic Immune Responses in Mice
    Article Snippet: Briefly, Sf9 cells cultured in 96-well plates at 2 × 106 cells/mL were infected with the recombinant baculovirus. .. After 48 h of infection, the cultured plates were fixed with 80% cold acetone overnight at -20°C, washed three times with PBS-0.05% Tween 20 (PBST), and then incubated with a rabbit anti-MERS-S polyclonal antibody (1:500, Sino Biological Inc, Beijing, China) containing 1% bovine serum albumin (BSA, Sigma-Aldrich, USA) at 37°C for 1 h. After three washes with PBST, an FITC-labeled goat against rabbit IgG antibody (1:300, BioWorld, Inc, St. Louis, MN, USA) was added with Evans blue (Sigma-Aldrich, St. Louis, MN, USA) for 1 h at 37 °C. ..

    Immunohistochemistry:

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease
    Article Snippet: .. To detect MERS-CoV antigen, IHC was performed using a rabbit polyclonal antiserum against MERS-CoV (Sino Biological PR China) (1:1000). .. Tissues from an uninfected control animal were used to validate all IHC procedures.

    Recombinant:

    Article Title: Development of a SERS-based lateral flow immunoassay for rapid and ultra-sensitive detection of anti-SARS-CoV-2 IgM/IgG in clinical samples
    Article Snippet: 2.1 Materials and chemicals Tetraethyl orthosilicate (TEOS), 2-(N-morpholino) ethanesulfonic (MES), polyethyleneimine (PEI, 25 K), polyvinylpyrrolidone (PVP, 40 K), Tween 20, N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), sodium borohydride (NaBH4 ), trisodium citrate (Na3 C6 H5 O7 , TSC), goat anti-human IgM, goat anti-human IgG, goat anti-rabbit IgG, bovine serum albumin (BSA) were obtained from Sigma-Aldrich (USA). .. SARS-CoV-2 S protein (recombinant) and S protein antibody (Ab) were obtained from Sino Biological Inc. (Beijing, China). .. Chloroauric acid tetrahydrate, sodium borohydride, formaldehyde (37%, w/w), ammonia solution (28%, w/w), chloroauric acid tetrahydrate (HAuCl4 ·4H2 O), and silver nitrate (AgNO3) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

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  • 95
    Sino Biological rabbit polyclonal antiserum against mers cov
    Sequencing of <t>MERS-CoV</t> Spike protein and data analysis
    Rabbit Polyclonal Antiserum Against Mers Cov, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antiserum against mers cov/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antiserum against mers cov - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    Sequencing of MERS-CoV Spike protein and data analysis

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Sequencing of MERS-CoV Spike protein and data analysis

    Article Snippet: To detect MERS-CoV antigen, IHC was performed using a rabbit polyclonal antiserum against MERS-CoV (Sino Biological PR China) (1:1000).

    Techniques: Sequencing

    Gross and Histopathology. (A) Gross lung pathology demonstrating mostly normal lung with multifocal to coalescing moderate interstitial pneumonia in the left caudal lung lobe. (B) Low magnification of lung field from MERS-CoV EMC inoculated subject demonstrating

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Gross and Histopathology. (A) Gross lung pathology demonstrating mostly normal lung with multifocal to coalescing moderate interstitial pneumonia in the left caudal lung lobe. (B) Low magnification of lung field from MERS-CoV EMC inoculated subject demonstrating

    Article Snippet: To detect MERS-CoV antigen, IHC was performed using a rabbit polyclonal antiserum against MERS-CoV (Sino Biological PR China) (1:1000).

    Techniques: Histopathology

    Ex-vivo analysis of primary cells. Cells were isolated from lung, kidney, and bronchoalveolar lavage (BAL), and one-step growth kinetics were performed as described in Materials and Methods. Lung, Kidney and BAL demonstrate that MERS-CoV is able to replicate

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Ex-vivo analysis of primary cells. Cells were isolated from lung, kidney, and bronchoalveolar lavage (BAL), and one-step growth kinetics were performed as described in Materials and Methods. Lung, Kidney and BAL demonstrate that MERS-CoV is able to replicate

    Article Snippet: To detect MERS-CoV antigen, IHC was performed using a rabbit polyclonal antiserum against MERS-CoV (Sino Biological PR China) (1:1000).

    Techniques: Ex Vivo, Isolation

    Fluorescence Reduction Neutralizing Assay. Sera from all subjects was evaluated for the presence of neutralizing antibody to MERS-CoV as described in Materials and Methods. Neutralizing antibody could be detected above the background of the Mock infected

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Fluorescence Reduction Neutralizing Assay. Sera from all subjects was evaluated for the presence of neutralizing antibody to MERS-CoV as described in Materials and Methods. Neutralizing antibody could be detected above the background of the Mock infected

    Article Snippet: To detect MERS-CoV antigen, IHC was performed using a rabbit polyclonal antiserum against MERS-CoV (Sino Biological PR China) (1:1000).

    Techniques: Fluorescence, Neutralizing Assay, Infection