Journal: British Journal of Cancer
Article Title: Curcumin–cyclodextrin complexes potentiate gemcitabine effects in an orthotopic mouse model of lung cancer
doi: 10.1038/bjc.2012.379
Figure Lengend Snippet: Curcumin–CD complexes affect the production of proteins related to apoptosis and cell cycle. ( A ) Western blot analyses have been performed to measure cleaved PARP, phospho-cyclin B1 and phospho-cdc2 levels. Actin is shown as loading control. ( B – D ) Quantification was performed by densitometric scanning. Results are expressed as mean±s.e.m. and are representative of two independent experiments.
Article Snippet: After blocking in phosphate-buffered saline containing 10% non-fat milk and Tween 20 (0.05%), membranes were incubated with the primary antibody: anti-poly (ADP-ribose) polymerase (PARP) rabbit monoclonal antibody, anti-cyclin B1 mouse monoclonal antibody, antiphospho-cyclin B1 rabbit polyclonal antibody and antiphospho-cdc2 (Thr161) rabbit polyclonal antibody (Cell Signaling, Leiden, The Netherlands).
Techniques: Western Blot, Control