rabbit polyclonal antibody  (Alomone Labs)


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    Alomone Labs rabbit polyclonal antibody
    Rabbit Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibody - by Bioz Stars, 2022-11
    93/100 stars

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    Alomone Labs anti tmem16a antibody
    Two different <t>TMEM16A‐specific</t> blockers abolished STICs and STDs. (a) an example showing the effect of 1 μM Ani9 and 3 μM CaCC(inh)‐A01 on STICs held under voltage clamp at −60 mV. (bi) summary of the effect of 1 μM Ani9 on STIC amplitude ( n = 6 cells from 6 animals; * p
    Anti Tmem16a Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tmem16a antibody/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    95
    Alomone Labs anti nav1 6 scn8a antibody
    Endogenously tagged Na V 1.2 displays higher mobility at the distal AIS ( A ) FRAP of Na V 1.2-GFP knock-in neurons. Still images of a <t>Nav1.2-GFP</t> knock-in neuron before (–5 min), and 0 min and 45 min after FRAP in the proximal and distal area (A), FRAP ROIs are indicated by white arrowheads. ( B ) Corresponding kymograph from experiment in A. ( C ) Average fluorescence recovery in the proximal and distal AIS of 15 neurons, from 2 independent experiments. 2-way ANOVA with Šídák’s multiple comparisons test, 44 min: *p = 0.02, 45 min: *p = 0.04. ( D ). Individual recovery in the proximal versus distal AIS, paired t-test **p = 0.002, n = 15 neurons from 2 independent experiments.
    Anti Nav1 6 Scn8a Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nav1 6 scn8a antibody/product/Alomone Labs
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    95
    Alomone Labs anti kir4 1 kcnj10 antibody
    Endogenously tagged Na V 1.2 displays higher mobility at the distal AIS ( A ) FRAP of Na V 1.2-GFP knock-in neurons. Still images of a <t>Nav1.2-GFP</t> knock-in neuron before (–5 min), and 0 min and 45 min after FRAP in the proximal and distal area (A), FRAP ROIs are indicated by white arrowheads. ( B ) Corresponding kymograph from experiment in A. ( C ) Average fluorescence recovery in the proximal and distal AIS of 15 neurons, from 2 independent experiments. 2-way ANOVA with Šídák’s multiple comparisons test, 44 min: *p = 0.02, 45 min: *p = 0.04. ( D ). Individual recovery in the proximal versus distal AIS, paired t-test **p = 0.002, n = 15 neurons from 2 independent experiments.
    Anti Kir4 1 Kcnj10 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Alomone Labs anti mc5 receptor antibody
    Endogenously tagged Na V 1.2 displays higher mobility at the distal AIS ( A ) FRAP of Na V 1.2-GFP knock-in neurons. Still images of a <t>Nav1.2-GFP</t> knock-in neuron before (–5 min), and 0 min and 45 min after FRAP in the proximal and distal area (A), FRAP ROIs are indicated by white arrowheads. ( B ) Corresponding kymograph from experiment in A. ( C ) Average fluorescence recovery in the proximal and distal AIS of 15 neurons, from 2 independent experiments. 2-way ANOVA with Šídák’s multiple comparisons test, 44 min: *p = 0.02, 45 min: *p = 0.04. ( D ). Individual recovery in the proximal versus distal AIS, paired t-test **p = 0.002, n = 15 neurons from 2 independent experiments.
    Anti Mc5 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Two different TMEM16A‐specific blockers abolished STICs and STDs. (a) an example showing the effect of 1 μM Ani9 and 3 μM CaCC(inh)‐A01 on STICs held under voltage clamp at −60 mV. (bi) summary of the effect of 1 μM Ani9 on STIC amplitude ( n = 6 cells from 6 animals; * p

    Journal: Physiological Reports

    Article Title: Ca2+‐activated Cl− channels ( TMEM16A) underlie spontaneous electrical activity in isolated mouse corpus cavernosum smooth muscle cells). Ca2+‐activated Cl− channels ( TMEM16A) underlie spontaneous electrical activity in isolated mouse corpus cavernosum smooth muscle cells

    doi: 10.14814/phy2.15504

    Figure Lengend Snippet: Two different TMEM16A‐specific blockers abolished STICs and STDs. (a) an example showing the effect of 1 μM Ani9 and 3 μM CaCC(inh)‐A01 on STICs held under voltage clamp at −60 mV. (bi) summary of the effect of 1 μM Ani9 on STIC amplitude ( n = 6 cells from 6 animals; * p

    Article Snippet: The only primary antibody used was the anti‐TMEM16A antibody (rabbit polyclonal anti‐human, 1:200 dilution, Alomone Labs Cat# ab72984).

    Techniques:

    Immunofluorescence detection of TMEM16A in freshly isolated CCSM cells. (ai) representative confocal photomicrograph showing that freshly isolated mouse CCSM cells were immunoreactive to a selective TMEM16A antibody, demonstrated by the strong immunofluorescence signal detected [green] ( n = 3 from 3 animals). (aii) bright field image of the same cell as in ai. (bi) negative control, representative confocal photomicrograph showing no immunofluorescence detected in detrusor SM cell exposed to the TMEM16A antibody ( n = 3 from 3 animals). (bii) bright field image of the same cell as in bi. (c) Positive control, representative confocal photomicrographs showing robust TMEM16A staining in mouse jejunum tissue strip ( n = 3 from 3 animals). (d) Smooth muscle cells were identified by smooth muscle myosin positive immunoreactivity [green] ( n = 3 from 3 animals)

    Journal: Physiological Reports

    Article Title: Ca2+‐activated Cl− channels ( TMEM16A) underlie spontaneous electrical activity in isolated mouse corpus cavernosum smooth muscle cells). Ca2+‐activated Cl− channels ( TMEM16A) underlie spontaneous electrical activity in isolated mouse corpus cavernosum smooth muscle cells

    doi: 10.14814/phy2.15504

    Figure Lengend Snippet: Immunofluorescence detection of TMEM16A in freshly isolated CCSM cells. (ai) representative confocal photomicrograph showing that freshly isolated mouse CCSM cells were immunoreactive to a selective TMEM16A antibody, demonstrated by the strong immunofluorescence signal detected [green] ( n = 3 from 3 animals). (aii) bright field image of the same cell as in ai. (bi) negative control, representative confocal photomicrograph showing no immunofluorescence detected in detrusor SM cell exposed to the TMEM16A antibody ( n = 3 from 3 animals). (bii) bright field image of the same cell as in bi. (c) Positive control, representative confocal photomicrographs showing robust TMEM16A staining in mouse jejunum tissue strip ( n = 3 from 3 animals). (d) Smooth muscle cells were identified by smooth muscle myosin positive immunoreactivity [green] ( n = 3 from 3 animals)

    Article Snippet: The only primary antibody used was the anti‐TMEM16A antibody (rabbit polyclonal anti‐human, 1:200 dilution, Alomone Labs Cat# ab72984).

    Techniques: Immunofluorescence, Isolation, Negative Control, Positive Control, Staining, Stripping Membranes

    Two different TMEM16A‐specific blockers inhibited chloride tail currents. (ai) the cell was held at −60 mV and stepped to 0 mV. It was then stepped down to −80 mV to evoke a tail current. Representative traces showing the reversible effect of 1 μM Ani9 on tail current. (aii) summary data showing the effect of 1 μM Ani9 on tail current ( n = 6 cells from 6 animals, * p

    Journal: Physiological Reports

    Article Title: Ca2+‐activated Cl− channels ( TMEM16A) underlie spontaneous electrical activity in isolated mouse corpus cavernosum smooth muscle cells). Ca2+‐activated Cl− channels ( TMEM16A) underlie spontaneous electrical activity in isolated mouse corpus cavernosum smooth muscle cells

    doi: 10.14814/phy2.15504

    Figure Lengend Snippet: Two different TMEM16A‐specific blockers inhibited chloride tail currents. (ai) the cell was held at −60 mV and stepped to 0 mV. It was then stepped down to −80 mV to evoke a tail current. Representative traces showing the reversible effect of 1 μM Ani9 on tail current. (aii) summary data showing the effect of 1 μM Ani9 on tail current ( n = 6 cells from 6 animals, * p

    Article Snippet: The only primary antibody used was the anti‐TMEM16A antibody (rabbit polyclonal anti‐human, 1:200 dilution, Alomone Labs Cat# ab72984).

    Techniques:

    Endogenously tagged Na V 1.2 displays higher mobility at the distal AIS ( A ) FRAP of Na V 1.2-GFP knock-in neurons. Still images of a Nav1.2-GFP knock-in neuron before (–5 min), and 0 min and 45 min after FRAP in the proximal and distal area (A), FRAP ROIs are indicated by white arrowheads. ( B ) Corresponding kymograph from experiment in A. ( C ) Average fluorescence recovery in the proximal and distal AIS of 15 neurons, from 2 independent experiments. 2-way ANOVA with Šídák’s multiple comparisons test, 44 min: *p = 0.02, 45 min: *p = 0.04. ( D ). Individual recovery in the proximal versus distal AIS, paired t-test **p = 0.002, n = 15 neurons from 2 independent experiments.

    Journal: bioRxiv

    Article Title: Sodium channel endocytosis drives axon initial segment plasticity

    doi: 10.1101/2022.11.09.515770

    Figure Lengend Snippet: Endogenously tagged Na V 1.2 displays higher mobility at the distal AIS ( A ) FRAP of Na V 1.2-GFP knock-in neurons. Still images of a Nav1.2-GFP knock-in neuron before (–5 min), and 0 min and 45 min after FRAP in the proximal and distal area (A), FRAP ROIs are indicated by white arrowheads. ( B ) Corresponding kymograph from experiment in A. ( C ) Average fluorescence recovery in the proximal and distal AIS of 15 neurons, from 2 independent experiments. 2-way ANOVA with Šídák’s multiple comparisons test, 44 min: *p = 0.02, 45 min: *p = 0.04. ( D ). Individual recovery in the proximal versus distal AIS, paired t-test **p = 0.002, n = 15 neurons from 2 independent experiments.

    Article Snippet: Antibodies The following antibodies were used in this study: NaV 1.2 (NeuroMab/Antibodies Incorporated #75-024), NaV1.6 (Alomone Labs #ASC-009 and NeuroMab/Antibodies Incorporated #73-026), Pan-Nav (Sigma, #S8809), AnkG (Life technologies #33-8800, Neuromab/Antibodies Incorporated #75-146 and Synaptic systems #386-005), βIV-spectrin (Biotrend, provided by Maren Engelhardt, Johnnes-Kepler-University, Linz, Austria), MAP2 (Abcam/Bio Connect #ab5392), GFP (MBL International/Sanbio #598 and Abcam #ab13970).Corresponding secondary antibodies Alexa-conjugated 405, 488, 568, 594 or 647 goat anti-mouse, anti-mouse IgG1/IgG2a, anti-rabbit, anti guinea-pig or anti-chicken were used (Life Technologies), as well as streptavidin Alexa-633 conjugate (Life Technologies) for the detection of biocytin filled neurons.

    Techniques: Knock-In, Fluorescence

    Plasticity-induced Na V 1.2 removal is mediated by endocytosis ( A ) Immunostaining for Na V 1.2, AnkG and MAP2 of DIV14 neurons in control conditions and c-LTD with or without the dynamin inhibitor dynasore. ( B ) Na V 1.2 and ( C ) AnkG relative length following c-LTD and in the presence of MG-132, MDL2870, dynasore and Pitstop 2, N = 3-6 independent experiments and n > 100 neurons per condition per experiment. Kruskal-Wallis test; c-LTD: Nav1.2 *p = 0.019, AnkG **p = 0.001, c-LTD + MG-132: Nav1.2 *p = 0.036, AnkG ns p = 0.21, c-LTD + MDL28170: Na V 1.2 **p = 0.005, AnkG *p = 0.018, c-LTD + dynasore: Na V 1.2 ns p > 0.99, AnkG ns p = 0.38, c-LTD + Pitstop 2: Na V 1.2 ns p = 0.068, AnkG ns p = 0.39. ( D ) Immunostaining for dynamin2 reveals increased NMDA-induced dynamin clusters in the AIS. Scale bar is 20 μm. ( E, F ) Population analysis of dynamin2 cluster areas and dynamin surface in the Na V 1.2-positive AIS. In (E): paired t-test, *p = 0.035 and in (F): paired t-test, *p = 0.033. N = 3 independent experiments, from 4 to 7 neurons per condition per experiment. ( G ) Summary scheme and working model. Synaptic NMDARs activation and APs generate local Ca 2+ influx activating downstream effectors, including calcineurin and lead to AnkG degradation by the UPS and local recruitment of dynamin2 responsible for Na V 1.2 channels internalization.

    Journal: bioRxiv

    Article Title: Sodium channel endocytosis drives axon initial segment plasticity

    doi: 10.1101/2022.11.09.515770

    Figure Lengend Snippet: Plasticity-induced Na V 1.2 removal is mediated by endocytosis ( A ) Immunostaining for Na V 1.2, AnkG and MAP2 of DIV14 neurons in control conditions and c-LTD with or without the dynamin inhibitor dynasore. ( B ) Na V 1.2 and ( C ) AnkG relative length following c-LTD and in the presence of MG-132, MDL2870, dynasore and Pitstop 2, N = 3-6 independent experiments and n > 100 neurons per condition per experiment. Kruskal-Wallis test; c-LTD: Nav1.2 *p = 0.019, AnkG **p = 0.001, c-LTD + MG-132: Nav1.2 *p = 0.036, AnkG ns p = 0.21, c-LTD + MDL28170: Na V 1.2 **p = 0.005, AnkG *p = 0.018, c-LTD + dynasore: Na V 1.2 ns p > 0.99, AnkG ns p = 0.38, c-LTD + Pitstop 2: Na V 1.2 ns p = 0.068, AnkG ns p = 0.39. ( D ) Immunostaining for dynamin2 reveals increased NMDA-induced dynamin clusters in the AIS. Scale bar is 20 μm. ( E, F ) Population analysis of dynamin2 cluster areas and dynamin surface in the Na V 1.2-positive AIS. In (E): paired t-test, *p = 0.035 and in (F): paired t-test, *p = 0.033. N = 3 independent experiments, from 4 to 7 neurons per condition per experiment. ( G ) Summary scheme and working model. Synaptic NMDARs activation and APs generate local Ca 2+ influx activating downstream effectors, including calcineurin and lead to AnkG degradation by the UPS and local recruitment of dynamin2 responsible for Na V 1.2 channels internalization.

    Article Snippet: Antibodies The following antibodies were used in this study: NaV 1.2 (NeuroMab/Antibodies Incorporated #75-024), NaV1.6 (Alomone Labs #ASC-009 and NeuroMab/Antibodies Incorporated #73-026), Pan-Nav (Sigma, #S8809), AnkG (Life technologies #33-8800, Neuromab/Antibodies Incorporated #75-146 and Synaptic systems #386-005), βIV-spectrin (Biotrend, provided by Maren Engelhardt, Johnnes-Kepler-University, Linz, Austria), MAP2 (Abcam/Bio Connect #ab5392), GFP (MBL International/Sanbio #598 and Abcam #ab13970).Corresponding secondary antibodies Alexa-conjugated 405, 488, 568, 594 or 647 goat anti-mouse, anti-mouse IgG1/IgG2a, anti-rabbit, anti guinea-pig or anti-chicken were used (Life Technologies), as well as streptavidin Alexa-633 conjugate (Life Technologies) for the detection of biocytin filled neurons.

    Techniques: Immunostaining, Activation Assay