rabbit polyclonal antibodies against mas receptor  (Alomone Labs)

Journal: Molecular Pain

Article Title: Activation of mammalian target of rapamycin mediates rat pain-related responses induced by BmK I, a sodium channel-specific modulator

doi: 10.1186/1744-8069-9-50

Figure Lengend Snippet: Immunoreactivity of p-p70 S6K in the spinal cord dorsal horn after intraplantar BmK I injection. Compared with the saline group (A , B) , the immunoreactivity of p-p70 S6K in both sides of spinal cord dorsal horn increased significantly in BmK I treated groups (C - F) .

Article Snippet: The membranes were incubated with the following primary antibodies which were diluted in PBS-T containing 5% nonfat milk for 5 h at room temperature: rabbit polyclonal antibody against p-mTOR (Ser2448, 1:1000; #2971; Cell Signaling Technology, MA [ ])/rabbit monoclonal antibody against mTOR (7C10, 1:1200; #2983; Cell Signaling Technology, MA)/rabbit polyclonal antibody against p-p70 S6K (Thr421/Ser424, 1:1500; sc-7984-R; Santa Cruz, CA [ ])/rabbit monoclonal antibody against p-4E-BP1 (Thr37/46, 1:1000; #2855; Cell Signaling Technology, MA)/rabbit polyclonal antibody against Actin (I-19, 1:1000; sc-1616; Santa Cruz, CA).

Techniques: Injection

Journal: Molecular Pain

Article Title: Activation of mammalian target of rapamycin mediates rat pain-related responses induced by BmK I, a sodium channel-specific modulator

doi: 10.1186/1744-8069-9-50

Figure Lengend Snippet: BmK I-induced changes in spinal levels of p-mTOR, p-4E-BP1 and p-p70 S6K assessed by Western blot. (A) & (F) , representative western blots showing levels of p-mTOR, p-4E-BP, p-p70 S6K, total mTOR and β-actin in both ipsi- (A) and contralateral (B) side of the spinal cord. (B - E) & (G - J) , histograms represent the mean levels with respect to each control group at different time points after intraplantar BmK I injection. The data are presented as mean ± S.E.M. of three rats per group. * p <0.05, ** p <0.01, *** p <0.001, compared with control group by One-way ANOVA, followed by Bonferroni's post hoc test.

Article Snippet: The membranes were incubated with the following primary antibodies which were diluted in PBS-T containing 5% nonfat milk for 5 h at room temperature: rabbit polyclonal antibody against p-mTOR (Ser2448, 1:1000; #2971; Cell Signaling Technology, MA [ ])/rabbit monoclonal antibody against mTOR (7C10, 1:1200; #2983; Cell Signaling Technology, MA)/rabbit polyclonal antibody against p-p70 S6K (Thr421/Ser424, 1:1500; sc-7984-R; Santa Cruz, CA [ ])/rabbit monoclonal antibody against p-4E-BP1 (Thr37/46, 1:1000; #2855; Cell Signaling Technology, MA)/rabbit polyclonal antibody against Actin (I-19, 1:1000; sc-1616; Santa Cruz, CA).

Techniques: Western Blot, Injection

Journal: Molecular Pain

Article Title: Activation of mammalian target of rapamycin mediates rat pain-related responses induced by BmK I, a sodium channel-specific modulator

doi: 10.1186/1744-8069-9-50

Figure Lengend Snippet: Cellular localization of p-p70 S6K immunoreactivity in the ipsilateral spinal cord dorsal horn. (A - I) Cell-type-specific immunolabeling of p-p70 S6K in the ipsilateral dorsal horn at 2 h after intraplantar BmK I. Arrows indicate colocalization (yellow) of the p-p70 S6K (red) with the respective cell markers (green). (J) Statistical histogram of the cellular distribution of p-p70 S6K.

Article Snippet: The membranes were incubated with the following primary antibodies which were diluted in PBS-T containing 5% nonfat milk for 5 h at room temperature: rabbit polyclonal antibody against p-mTOR (Ser2448, 1:1000; #2971; Cell Signaling Technology, MA [ ])/rabbit monoclonal antibody against mTOR (7C10, 1:1200; #2983; Cell Signaling Technology, MA)/rabbit polyclonal antibody against p-p70 S6K (Thr421/Ser424, 1:1500; sc-7984-R; Santa Cruz, CA [ ])/rabbit monoclonal antibody against p-4E-BP1 (Thr37/46, 1:1000; #2855; Cell Signaling Technology, MA)/rabbit polyclonal antibody against Actin (I-19, 1:1000; sc-1616; Santa Cruz, CA).

Techniques: Immunolabeling

Journal: Molecular Pain

Article Title: Activation of mammalian target of rapamycin mediates rat pain-related responses induced by BmK I, a sodium channel-specific modulator

doi: 10.1186/1744-8069-9-50

Figure Lengend Snippet: 200 μM rapamycin inhibited the activation of spinal mTOR, 4E-BP1 and p70 S6K assessed by Western blot. (A) & (F) , representative western blots showing levels of p-mTOR, p-4E-BP, p-p70 S6K, total mTOR and β-actin in ipsi- (A) and contralateral (B) side of the spinal cord. (B - E) & (G - J) , histograms represent the mean levels with respect to each saline-treated group at 2 h after i.pl. BmK I injection. The data are presented as mean ± S.E.M. of three rats per group. N.s., ** p <0.01, *** p <0.001, compared with saline-treated group by One-way ANOVA, followed by Bonferroni's post hoc test.

Article Snippet: The membranes were incubated with the following primary antibodies which were diluted in PBS-T containing 5% nonfat milk for 5 h at room temperature: rabbit polyclonal antibody against p-mTOR (Ser2448, 1:1000; #2971; Cell Signaling Technology, MA [ ])/rabbit monoclonal antibody against mTOR (7C10, 1:1200; #2983; Cell Signaling Technology, MA)/rabbit polyclonal antibody against p-p70 S6K (Thr421/Ser424, 1:1500; sc-7984-R; Santa Cruz, CA [ ])/rabbit monoclonal antibody against p-4E-BP1 (Thr37/46, 1:1000; #2855; Cell Signaling Technology, MA)/rabbit polyclonal antibody against Actin (I-19, 1:1000; sc-1616; Santa Cruz, CA).

Techniques: Activation Assay, Western Blot, Injection