Journal: Cell

Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

doi: 10.1016/j.cell.2017.04.034

Figure Lengend Snippet: (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).

Article Snippet: Rabbit polyclonal anti-ub-H2B , Cell Signaling Technology , Cat#5546; RRID:AB_10693452.

Techniques: Western Blot

Journal: Cell

Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

doi: 10.1016/j.cell.2017.04.034

Figure Lengend Snippet: (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.

Article Snippet: Rabbit polyclonal anti-ub-H2B , Cell Signaling Technology , Cat#5546; RRID:AB_10693452.

Techniques: Immunostaining, Staining, Transduction, Construct, Translocation Assay

Journal: Cell

Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

doi: 10.1016/j.cell.2017.04.034

Figure Lengend Snippet: Key Resources Table

Article Snippet: Rabbit polyclonal anti-ub-H2B , Cell Signaling Technology , Cat#5546; RRID:AB_10693452.

Techniques: Recombinant, In Situ, Sequencing, Software