rabbit polyclonal anti trpv2 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal anti trpv2 antibody
    Light stimulation promotes cell migration mediated by <t>TRPV2</t> in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment (n=432), co-transfected with TRPV2[E594K] (n=290), treated with DMSO 1/1000 (n=180), treated with 100 µM Tranilast (n=285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast (n=89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p
    Rabbit Polyclonal Anti Trpv2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti trpv2 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti trpv2 antibody - by Bioz Stars, 2022-01
    95/100 stars

    Images

    1) Product Images from "Fine tuning of calcium constitutive entry by optogenetically-controlled membrane polarization: impact on cell migration"

    Article Title: Fine tuning of calcium constitutive entry by optogenetically-controlled membrane polarization: impact on cell migration

    Journal: bioRxiv

    doi: 10.1101/2020.06.04.134205

    Light stimulation promotes cell migration mediated by TRPV2 in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment (n=432), co-transfected with TRPV2[E594K] (n=290), treated with DMSO 1/1000 (n=180), treated with 100 µM Tranilast (n=285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast (n=89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p
    Figure Legend Snippet: Light stimulation promotes cell migration mediated by TRPV2 in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment (n=432), co-transfected with TRPV2[E594K] (n=290), treated with DMSO 1/1000 (n=180), treated with 100 µM Tranilast (n=285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast (n=89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p

    Techniques Used: Migration, Expressing, Transfection

    Expression of TRPV2 channels in C2C12 myoblasts. ( A ) Assessment of TRPV2 mRNA expression in C2C12 myoblasts by RT-PCR. ( B ) Western blot analysis of TRPV2 and GAPDH expression in C2C12 myoblasts, HEK293 cells stably expressing TRPV2 (HEK TRPV2), and control HEK293 cells (HEK ctrl). TRPV2 and GAPDH were detected sequentially on the same blot (stripped twice). 5 µg of proteins were deposited for HEK cell lysates and 10 µg for C2C12 myoblasts (n=4). ( C ) Confocal images at two magnifications showing immuno-localization of TRPV2 (red) and nuclei staining (blue) in C2C12 myoblasts (scale bar: 20 µm).
    Figure Legend Snippet: Expression of TRPV2 channels in C2C12 myoblasts. ( A ) Assessment of TRPV2 mRNA expression in C2C12 myoblasts by RT-PCR. ( B ) Western blot analysis of TRPV2 and GAPDH expression in C2C12 myoblasts, HEK293 cells stably expressing TRPV2 (HEK TRPV2), and control HEK293 cells (HEK ctrl). TRPV2 and GAPDH were detected sequentially on the same blot (stripped twice). 5 µg of proteins were deposited for HEK cell lysates and 10 µg for C2C12 myoblasts (n=4). ( C ) Confocal images at two magnifications showing immuno-localization of TRPV2 (red) and nuclei staining (blue) in C2C12 myoblasts (scale bar: 20 µm).

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Stable Transfection, Staining

    Involvement of TRPV2 channels in the mediation of constitutive calcium entry during light stimulation. ( A ) Representative traces of Fura-2 normalized ratio in response to a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP treated with DMSO 1/1000 (vehicle) or with 100 µM Tranilast, a TRPV2 inhibitor. ( B ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in eNpHR-expressing C2C12 myoblasts treated with DMSO 1/1000 (n=40) or with 100 µM Tranilast (n=45). ( C ) Confocal images showing immuno-expression of the negative-dominant TRPV2[E594K] tagged with a flag sequence (red) co-transfected (TRPV2[E594K] right panels) or not (Ctrl, left panel) with eNpHR-YFP (green) in C2C12 myoblasts. ( D ) Representative traces of normalized Fura-2 ratio during a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP co-transfected (TRPV2[E594K]) or not (Ctrl) with the negative-dominant TRPV2[E594K]. ( E ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in control C2C12 myoblasts expressing eNpHR-YFP (Ctrl, n=31) or in C2C12 myoblasts expressing eNpHR-YFP co-transfected with negative-dominant TRPV2[E594K] (TRPV2[E594K], n=102). Data are presented as mean ± SEM. *** and **** represent significant differences with p
    Figure Legend Snippet: Involvement of TRPV2 channels in the mediation of constitutive calcium entry during light stimulation. ( A ) Representative traces of Fura-2 normalized ratio in response to a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP treated with DMSO 1/1000 (vehicle) or with 100 µM Tranilast, a TRPV2 inhibitor. ( B ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in eNpHR-expressing C2C12 myoblasts treated with DMSO 1/1000 (n=40) or with 100 µM Tranilast (n=45). ( C ) Confocal images showing immuno-expression of the negative-dominant TRPV2[E594K] tagged with a flag sequence (red) co-transfected (TRPV2[E594K] right panels) or not (Ctrl, left panel) with eNpHR-YFP (green) in C2C12 myoblasts. ( D ) Representative traces of normalized Fura-2 ratio during a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP co-transfected (TRPV2[E594K]) or not (Ctrl) with the negative-dominant TRPV2[E594K]. ( E ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in control C2C12 myoblasts expressing eNpHR-YFP (Ctrl, n=31) or in C2C12 myoblasts expressing eNpHR-YFP co-transfected with negative-dominant TRPV2[E594K] (TRPV2[E594K], n=102). Data are presented as mean ± SEM. *** and **** represent significant differences with p

    Techniques Used: Expressing, Fluorescence, Sequencing, Transfection

    Schematic model of the effect of light-induced activation of halorhodopsin pump on the activation of calcium constitutive entry pathway through TRPV2 channels and on the modulation of cell migration. ( 1 ) Light stimulation of C2C12 myoblasts expressing eNpHR (orange) leads to membrane polarization by chloride ion entry (yellow) which gives rise to constitutive calcium entry (red) through TRPV2 channel (blue) by increasing the driving force for Ca 2+ across the plasma membrane. This light-induced calcium entry increases cell migration in a manner that can be abolished ( 2 ) by the TRPV2 inhibitor Tranilast (purple) and the negative-dominant TRPV2[E594K] transcript (green).
    Figure Legend Snippet: Schematic model of the effect of light-induced activation of halorhodopsin pump on the activation of calcium constitutive entry pathway through TRPV2 channels and on the modulation of cell migration. ( 1 ) Light stimulation of C2C12 myoblasts expressing eNpHR (orange) leads to membrane polarization by chloride ion entry (yellow) which gives rise to constitutive calcium entry (red) through TRPV2 channel (blue) by increasing the driving force for Ca 2+ across the plasma membrane. This light-induced calcium entry increases cell migration in a manner that can be abolished ( 2 ) by the TRPV2 inhibitor Tranilast (purple) and the negative-dominant TRPV2[E594K] transcript (green).

    Techniques Used: Activation Assay, Migration, Expressing

    2) Product Images from "Fine Tuning of Calcium Constitutive Entry by Optogenetically-Controlled Membrane Polarization: Impact on Cell Migration"

    Article Title: Fine Tuning of Calcium Constitutive Entry by Optogenetically-Controlled Membrane Polarization: Impact on Cell Migration

    Journal: Cells

    doi: 10.3390/cells9071684

    Involvement of TRPV2 channels in the mediation of constitutive calcium entry during light stimulation. ( A ) Representative traces of Fura-2 normalized ratio in response to a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP treated with DMSO 1/1000 (vehicle) or with 100 µM Tranilast, a TRPV2 inhibitor. ( B ) Maximum amplitude (ΔF/F0) of the Fura-2 fluorescence response to light stimulation in eNpHR-expressing C2C12 myoblasts treated with DMSO 1/1000 ( n =4 0) or with 100 µM Tranilast ( n = 45). ( C ) Confocal images showing immuno-expression of the negative-dominant TRPV2[E594K] tagged with a flag sequence (red) co-transfected (TRPV2[E594K] right panels) or not (Ctrl, left panel) with eNpHR-YFP (green) in C2C12 myoblasts. ( D ) Representative traces of normalized Fura-2 ratio during a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP co-transfected (TRPV2[E594K]) or not (Ctrl) with the negative-dominant TRPV2[E594K]. ( E ) Maximum amplitude (ΔF/F0) of the Fura-2 fluorescence response to light stimulation in control C2C12 myoblasts expressing eNpHR-YFP (Ctrl, n = 31) or in C2C12 myoblasts expressing eNpHR-YFP co-transfected with negative-dominant TRPV2[E594K] (TRPV2[E594K], n = 102). Data are presented as mean ± SEM. *** and **** represent significant differences with p
    Figure Legend Snippet: Involvement of TRPV2 channels in the mediation of constitutive calcium entry during light stimulation. ( A ) Representative traces of Fura-2 normalized ratio in response to a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP treated with DMSO 1/1000 (vehicle) or with 100 µM Tranilast, a TRPV2 inhibitor. ( B ) Maximum amplitude (ΔF/F0) of the Fura-2 fluorescence response to light stimulation in eNpHR-expressing C2C12 myoblasts treated with DMSO 1/1000 ( n =4 0) or with 100 µM Tranilast ( n = 45). ( C ) Confocal images showing immuno-expression of the negative-dominant TRPV2[E594K] tagged with a flag sequence (red) co-transfected (TRPV2[E594K] right panels) or not (Ctrl, left panel) with eNpHR-YFP (green) in C2C12 myoblasts. ( D ) Representative traces of normalized Fura-2 ratio during a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP co-transfected (TRPV2[E594K]) or not (Ctrl) with the negative-dominant TRPV2[E594K]. ( E ) Maximum amplitude (ΔF/F0) of the Fura-2 fluorescence response to light stimulation in control C2C12 myoblasts expressing eNpHR-YFP (Ctrl, n = 31) or in C2C12 myoblasts expressing eNpHR-YFP co-transfected with negative-dominant TRPV2[E594K] (TRPV2[E594K], n = 102). Data are presented as mean ± SEM. *** and **** represent significant differences with p

    Techniques Used: Expressing, Fluorescence, Sequencing, Transfection

    Schematic model of the effect of light-induced activation of halorhodopsin pump on the activation of calcium constitutive entry pathway through TRPV2 channels and on the modulation of cell migration. ( 1 ) Light stimulation of C2C12 myoblasts expressing eNpHR (orange) leads to membrane polarization by chloride ion entry (yellow) which gives rise to constitutive calcium entry (red) through TRPV2 channel (blue) by increasing the driving force for Ca 2+ across the plasma membrane. This light-induced calcium entry increases cell migration in a manner that can be abolished ( 2 ) by the TRPV2 inhibitor Tranilast (purple) and the negative-dominant TRPV2[E594K] transcript (green).
    Figure Legend Snippet: Schematic model of the effect of light-induced activation of halorhodopsin pump on the activation of calcium constitutive entry pathway through TRPV2 channels and on the modulation of cell migration. ( 1 ) Light stimulation of C2C12 myoblasts expressing eNpHR (orange) leads to membrane polarization by chloride ion entry (yellow) which gives rise to constitutive calcium entry (red) through TRPV2 channel (blue) by increasing the driving force for Ca 2+ across the plasma membrane. This light-induced calcium entry increases cell migration in a manner that can be abolished ( 2 ) by the TRPV2 inhibitor Tranilast (purple) and the negative-dominant TRPV2[E594K] transcript (green).

    Techniques Used: Activation Assay, Migration, Expressing

    Expression of transient receptor potential vanilloid 2 (TRPV2) channels in C2C12 myoblasts. ( A ) Assessment of TRPV2 mRNA expression in C2C12 myoblasts by RT-PCR. ( B ) Western blot analysis of TRPV2 and GAPDH expression in C2C12 myoblasts, human embryonic kidney (HEK)293 cells stably expressing TRPV2 (HEK TRPV2), and control HEK293 cells (HEK ctrl). TRPV2 and GAPDH were detected sequentially on the same blot (stripped twice). 5 µg of proteins were deposited for HEK cell lysates and 10 µg for C2C12 myoblasts ( n = 4). ( C ) Confocal images at two magnifications showing immuno-localization of TRPV2 (red) and nuclei staining (blue) in C2C12 myoblasts (scale bar: 20 µm).
    Figure Legend Snippet: Expression of transient receptor potential vanilloid 2 (TRPV2) channels in C2C12 myoblasts. ( A ) Assessment of TRPV2 mRNA expression in C2C12 myoblasts by RT-PCR. ( B ) Western blot analysis of TRPV2 and GAPDH expression in C2C12 myoblasts, human embryonic kidney (HEK)293 cells stably expressing TRPV2 (HEK TRPV2), and control HEK293 cells (HEK ctrl). TRPV2 and GAPDH were detected sequentially on the same blot (stripped twice). 5 µg of proteins were deposited for HEK cell lysates and 10 µg for C2C12 myoblasts ( n = 4). ( C ) Confocal images at two magnifications showing immuno-localization of TRPV2 (red) and nuclei staining (blue) in C2C12 myoblasts (scale bar: 20 µm).

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Stable Transfection, Staining

    Light stimulation promotes cell migration mediated by TRPV2 in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment ( n = 432), co-transfected with TRPV2[E594K] ( n = 290), treated with DMSO 1/1000 ( n = 180), treated with 100 µM Tranilast ( n = 285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast ( n = 89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p
    Figure Legend Snippet: Light stimulation promotes cell migration mediated by TRPV2 in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment ( n = 432), co-transfected with TRPV2[E594K] ( n = 290), treated with DMSO 1/1000 ( n = 180), treated with 100 µM Tranilast ( n = 285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast ( n = 89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p

    Techniques Used: Migration, Expressing, Transfection

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    Alomone Labs rabbit polyclonal anti trpv2 antibody
    Light stimulation promotes cell migration mediated by <t>TRPV2</t> in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment (n=432), co-transfected with TRPV2[E594K] (n=290), treated with DMSO 1/1000 (n=180), treated with 100 µM Tranilast (n=285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast (n=89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p
    Rabbit Polyclonal Anti Trpv2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti trpv2 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti trpv2 antibody - by Bioz Stars, 2022-01
    95/100 stars
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    Light stimulation promotes cell migration mediated by TRPV2 in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment (n=432), co-transfected with TRPV2[E594K] (n=290), treated with DMSO 1/1000 (n=180), treated with 100 µM Tranilast (n=285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast (n=89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p

    Journal: bioRxiv

    Article Title: Fine tuning of calcium constitutive entry by optogenetically-controlled membrane polarization: impact on cell migration

    doi: 10.1101/2020.06.04.134205

    Figure Lengend Snippet: Light stimulation promotes cell migration mediated by TRPV2 in C2C12 myoblasts expressing eNpHR. ( A ) The role of TRPV2 channels in cell migration was evaluated with a cell migration tracking assay on C2C12 myoblasts expressing eNpHR. Cells were seeded at low density and the migration velocity (µm/min) was assessed for 15 h with a JuliStage system. C2C12 myoblast migration was evaluated for five conditions: without treatment (n=432), co-transfected with TRPV2[E594K] (n=290), treated with DMSO 1/1000 (n=180), treated with 100 µM Tranilast (n=285), and co-transfected with TRPV2[E594K] plus treated with 100 µM Tranilast (n=89) ($ corresponds to the comparison with C2C12 myoblasts without treatment, * corresponds to the comparison with C2C12 myoblasts treated with DMSO 1/1000) ($$$$ and ****: p

    Article Snippet: Cells were stained using the blocking solution containing rabbit polyclonal anti-TRPV2 antibody (1:200, ACC-039, Alomone labs) or rabbit polyclonal anti-FLAG antibody (1:100, F7425, Sigma-Aldrich) overnight at 4°C, followed by incubation with donkey anti-rabbit secondary antibody coupled with red fluorescent Alexa Fluor 555 (1:400, Molecular Probes, ThermoFisher Scientific) for 2 hours at room temperature.

    Techniques: Migration, Expressing, Transfection

    Expression of TRPV2 channels in C2C12 myoblasts. ( A ) Assessment of TRPV2 mRNA expression in C2C12 myoblasts by RT-PCR. ( B ) Western blot analysis of TRPV2 and GAPDH expression in C2C12 myoblasts, HEK293 cells stably expressing TRPV2 (HEK TRPV2), and control HEK293 cells (HEK ctrl). TRPV2 and GAPDH were detected sequentially on the same blot (stripped twice). 5 µg of proteins were deposited for HEK cell lysates and 10 µg for C2C12 myoblasts (n=4). ( C ) Confocal images at two magnifications showing immuno-localization of TRPV2 (red) and nuclei staining (blue) in C2C12 myoblasts (scale bar: 20 µm).

    Journal: bioRxiv

    Article Title: Fine tuning of calcium constitutive entry by optogenetically-controlled membrane polarization: impact on cell migration

    doi: 10.1101/2020.06.04.134205

    Figure Lengend Snippet: Expression of TRPV2 channels in C2C12 myoblasts. ( A ) Assessment of TRPV2 mRNA expression in C2C12 myoblasts by RT-PCR. ( B ) Western blot analysis of TRPV2 and GAPDH expression in C2C12 myoblasts, HEK293 cells stably expressing TRPV2 (HEK TRPV2), and control HEK293 cells (HEK ctrl). TRPV2 and GAPDH were detected sequentially on the same blot (stripped twice). 5 µg of proteins were deposited for HEK cell lysates and 10 µg for C2C12 myoblasts (n=4). ( C ) Confocal images at two magnifications showing immuno-localization of TRPV2 (red) and nuclei staining (blue) in C2C12 myoblasts (scale bar: 20 µm).

    Article Snippet: Cells were stained using the blocking solution containing rabbit polyclonal anti-TRPV2 antibody (1:200, ACC-039, Alomone labs) or rabbit polyclonal anti-FLAG antibody (1:100, F7425, Sigma-Aldrich) overnight at 4°C, followed by incubation with donkey anti-rabbit secondary antibody coupled with red fluorescent Alexa Fluor 555 (1:400, Molecular Probes, ThermoFisher Scientific) for 2 hours at room temperature.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Stable Transfection, Staining

    Involvement of TRPV2 channels in the mediation of constitutive calcium entry during light stimulation. ( A ) Representative traces of Fura-2 normalized ratio in response to a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP treated with DMSO 1/1000 (vehicle) or with 100 µM Tranilast, a TRPV2 inhibitor. ( B ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in eNpHR-expressing C2C12 myoblasts treated with DMSO 1/1000 (n=40) or with 100 µM Tranilast (n=45). ( C ) Confocal images showing immuno-expression of the negative-dominant TRPV2[E594K] tagged with a flag sequence (red) co-transfected (TRPV2[E594K] right panels) or not (Ctrl, left panel) with eNpHR-YFP (green) in C2C12 myoblasts. ( D ) Representative traces of normalized Fura-2 ratio during a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP co-transfected (TRPV2[E594K]) or not (Ctrl) with the negative-dominant TRPV2[E594K]. ( E ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in control C2C12 myoblasts expressing eNpHR-YFP (Ctrl, n=31) or in C2C12 myoblasts expressing eNpHR-YFP co-transfected with negative-dominant TRPV2[E594K] (TRPV2[E594K], n=102). Data are presented as mean ± SEM. *** and **** represent significant differences with p

    Journal: bioRxiv

    Article Title: Fine tuning of calcium constitutive entry by optogenetically-controlled membrane polarization: impact on cell migration

    doi: 10.1101/2020.06.04.134205

    Figure Lengend Snippet: Involvement of TRPV2 channels in the mediation of constitutive calcium entry during light stimulation. ( A ) Representative traces of Fura-2 normalized ratio in response to a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP treated with DMSO 1/1000 (vehicle) or with 100 µM Tranilast, a TRPV2 inhibitor. ( B ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in eNpHR-expressing C2C12 myoblasts treated with DMSO 1/1000 (n=40) or with 100 µM Tranilast (n=45). ( C ) Confocal images showing immuno-expression of the negative-dominant TRPV2[E594K] tagged with a flag sequence (red) co-transfected (TRPV2[E594K] right panels) or not (Ctrl, left panel) with eNpHR-YFP (green) in C2C12 myoblasts. ( D ) Representative traces of normalized Fura-2 ratio during a light stimulation of 30 s at 48 mW/cm 2 (orange rectangle) in C2C12 myoblasts expressing eNpHR-YFP co-transfected (TRPV2[E594K]) or not (Ctrl) with the negative-dominant TRPV2[E594K]. ( E ) Maximum amplitude (∆F/F0) of the Fura-2 fluorescence response to light stimulation in control C2C12 myoblasts expressing eNpHR-YFP (Ctrl, n=31) or in C2C12 myoblasts expressing eNpHR-YFP co-transfected with negative-dominant TRPV2[E594K] (TRPV2[E594K], n=102). Data are presented as mean ± SEM. *** and **** represent significant differences with p

    Article Snippet: Cells were stained using the blocking solution containing rabbit polyclonal anti-TRPV2 antibody (1:200, ACC-039, Alomone labs) or rabbit polyclonal anti-FLAG antibody (1:100, F7425, Sigma-Aldrich) overnight at 4°C, followed by incubation with donkey anti-rabbit secondary antibody coupled with red fluorescent Alexa Fluor 555 (1:400, Molecular Probes, ThermoFisher Scientific) for 2 hours at room temperature.

    Techniques: Expressing, Fluorescence, Sequencing, Transfection

    Schematic model of the effect of light-induced activation of halorhodopsin pump on the activation of calcium constitutive entry pathway through TRPV2 channels and on the modulation of cell migration. ( 1 ) Light stimulation of C2C12 myoblasts expressing eNpHR (orange) leads to membrane polarization by chloride ion entry (yellow) which gives rise to constitutive calcium entry (red) through TRPV2 channel (blue) by increasing the driving force for Ca 2+ across the plasma membrane. This light-induced calcium entry increases cell migration in a manner that can be abolished ( 2 ) by the TRPV2 inhibitor Tranilast (purple) and the negative-dominant TRPV2[E594K] transcript (green).

    Journal: bioRxiv

    Article Title: Fine tuning of calcium constitutive entry by optogenetically-controlled membrane polarization: impact on cell migration

    doi: 10.1101/2020.06.04.134205

    Figure Lengend Snippet: Schematic model of the effect of light-induced activation of halorhodopsin pump on the activation of calcium constitutive entry pathway through TRPV2 channels and on the modulation of cell migration. ( 1 ) Light stimulation of C2C12 myoblasts expressing eNpHR (orange) leads to membrane polarization by chloride ion entry (yellow) which gives rise to constitutive calcium entry (red) through TRPV2 channel (blue) by increasing the driving force for Ca 2+ across the plasma membrane. This light-induced calcium entry increases cell migration in a manner that can be abolished ( 2 ) by the TRPV2 inhibitor Tranilast (purple) and the negative-dominant TRPV2[E594K] transcript (green).

    Article Snippet: Cells were stained using the blocking solution containing rabbit polyclonal anti-TRPV2 antibody (1:200, ACC-039, Alomone labs) or rabbit polyclonal anti-FLAG antibody (1:100, F7425, Sigma-Aldrich) overnight at 4°C, followed by incubation with donkey anti-rabbit secondary antibody coupled with red fluorescent Alexa Fluor 555 (1:400, Molecular Probes, ThermoFisher Scientific) for 2 hours at room temperature.

    Techniques: Activation Assay, Migration, Expressing

    Influx in stretched cardiomyocytes in presence of TRPV2 channels antibodies

    Journal: Cell calcium

    Article Title: Axial stretch-dependent cation entry in dystrophic cardiomyopathy: Involvement of several TRPs channels

    doi: 10.1016/j.ceca.2016.01.001

    Figure Lengend Snippet: Influx in stretched cardiomyocytes in presence of TRPV2 channels antibodies

    Article Snippet: Immunolabelling with a-TRPV2 (ACC-039, Alomone lab) was performed using standard protocol including a permeabilization step with 0.1% Triton X-100 in PBS for 10 min.

    Techniques:

    TRPV2 protein expression in isolated WT and mdx cardiomyocytes

    Journal: Cell calcium

    Article Title: Axial stretch-dependent cation entry in dystrophic cardiomyopathy: Involvement of several TRPs channels

    doi: 10.1016/j.ceca.2016.01.001

    Figure Lengend Snippet: TRPV2 protein expression in isolated WT and mdx cardiomyocytes

    Article Snippet: Immunolabelling with a-TRPV2 (ACC-039, Alomone lab) was performed using standard protocol including a permeabilization step with 0.1% Triton X-100 in PBS for 10 min.

    Techniques: Expressing, Isolation

    TRPV2 expression in heart tissue from WT and mdx mice

    Journal: Cell calcium

    Article Title: Axial stretch-dependent cation entry in dystrophic cardiomyopathy: Involvement of several TRPs channels

    doi: 10.1016/j.ceca.2016.01.001

    Figure Lengend Snippet: TRPV2 expression in heart tissue from WT and mdx mice

    Article Snippet: Immunolabelling with a-TRPV2 (ACC-039, Alomone lab) was performed using standard protocol including a permeabilization step with 0.1% Triton X-100 in PBS for 10 min.

    Techniques: Expressing, Mouse Assay