Journal: Cell systems
Article Title: Pulse-chase proteomics of the App Knock-In mouse models of Alzheimer’s disease reveals synaptic dysfunction originates in presynaptic terminals
doi: 10.1016/j.cels.2020.11.007
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: The following primary antibodies were used for Western and dot blots: Anti-Human Amyloid-beta (N) (82E1) (also detects β-CTF) mouse monoclonal at 1:1,000 (Immuno-Biological Laboratories Cat# 10323, RRID: AB_10707424); Anti-Amyloid beta precursor protein [Y188] rabbit monoclonal at 1:1,000 (Abcam Cat# ab32136, RRID: AB_2289606); Anti-AP180 (Snap91) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 155 003, RRID: AB_887691); Anti-Gapdh (0411) mouse monoclonal at 1:2,000 (Santa Cruz Biotechnology Cat# sc-47724, RRID: AB_627678); Anti-Pip5k1c rabbit polyclonal at 1:500 (Novus Cat# NBP1–82986, RRID: AB_11029240); Anti-Snap25 rabbit polyclonal at 1:500 (Synaptic Systems Cat# 111 002, RRID: AB_887790); Anti-Synaptobrevin 2 (Vamp2) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 104 202, RRID: AB_887810); Anti-Synaptophysin mouse monoclonal at 1:1,1000 (Sigma-Aldrich Cat# S5768, RRID: AB_477523); Anti-Synaptotagmin 1/2 cytoplasmic tail rabbit polyclonal at 1:500 (Synaptic Systems Cat# 105 003AF, RRID: AB_2744565); Anti-Syntaxin 1B rabbit polyclonal at 1:500 (Synaptic Systems Cat# 110 402, RRID: AB_887901); Anti-Vamp1 rabbit polyclonal at 1:500 (Abcam Cat# ab41324, RRID: AB_1281203); Anti-Ubiquitin P4D1 mouse monoclonal at 1:1,000 (Santa Cruz Biotechnology Cat# sc-8017, RRID: AB_628423); Anti-VCP mouse monoclonal at 1:2,000 (Abcam Cat# ab11433, RRID: AB_298039).
Techniques: Recombinant, Sequencing, Modification, Blocking Assay, Bicinchoninic Acid Protein Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Mass Spectrometry