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rabbit polyclonal anti synaptotagmin 1 antibody  (Proteintech)

 
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    Structured Review

    Proteintech rabbit polyclonal anti synaptotagmin 1 antibody
    Rabbit Polyclonal Anti Synaptotagmin 1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti synaptotagmin 1 antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti synaptotagmin 1 antibody - by Bioz Stars, 2025-02
    86/100 stars

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    Figure 2A: <t>Anti-synaptotagmin-1</t> positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.
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    Figure 2A: <t>Anti-synaptotagmin-1</t> positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.
    Polyclonal Rabbit Anti Synaptotagmin 1 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam rabbit polyclonal anti synaptotagmin 1 antibody
    Figure 2A: <t>Anti-synaptotagmin-1</t> positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.
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    Abcam resource source identifier rabbit polyclonal anti-synaptotagmin 1 abcam cat#ab131551
    Figure 2A: <t>Anti-synaptotagmin-1</t> positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.
    Resource Source Identifier Rabbit Polyclonal Anti Synaptotagmin 1 Abcam Cat#Ab131551, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit polyclonal anti synaptotagmin 1 antibody
    Figure 2A: <t>Anti-synaptotagmin-1</t> positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.
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    Cell Signaling Technology Inc rabbit polyclonal anti synaptotagmin
    (a) neuronal maturation markers of cultured mouse cortical neurons (DIV7) ( top ) and hIPSC-derived neurons ( bottom ). Bright-field and merged fluorescence images of neuronal markers, β-tubulin III (TUBB3) and Map2 (MAP2), and synaptic proteins, postsynaptic density protein 95 (PSD95) and <t>synaptotagmin</t> I (SYT1), with DAPI (nuclei). Scale bar = 50 µm. Visualized dendritic branches and the presence of synaptic markers are characteristic of functional mature neurons. Scale bar = 50 µm. (b) Principal component analysis visualization of chromatin accessibility changes in hIPSC-induced neurons. (c) Number of DA-peaks recovered for each time point and stimulation category when compared versus control hIPSC-derived neurons. Gained (new) and closing (new) indicate peaks observed as differentially accessible for the first time in that time point, whereas gained and closing indicate DA-peaks already observed as DA in a previous time point. (d) Hierarchical clustering of normalized counts obtained from ATAC-seq consensus peaks ( left ) and only DA-peaks ( right ).
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    Synaptic Systems anti synaptotagmin 1 2 cytoplasmic tail rabbit polyclonal
    KEY RESOURCES TABLE
    Anti Synaptotagmin 1 2 Cytoplasmic Tail Rabbit Polyclonal, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Figure 2A: Anti-synaptotagmin-1 positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.

    Journal: PLoS ONE

    Article Title: Retinal Glycoprotein Enrichment by Concanavalin A Enabled Identification of Novel Membrane Autoantigen Synaptotagmin-1 in Equine Recurrent Uveitis

    doi: 10.1371/journal.pone.0050929

    Figure Lengend Snippet: Figure 2A: Anti-synaptotagmin-1 positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.

    Article Snippet: Using polyclonal rabbit anti-synaptotagmin-1 (dilution 1∶1500; Abcam) and monoclonal mouse anti Glucose-regulated protein 78 (GRP78) antibody (dilution 1∶50, BD Biosciences, Heidelberg, Germany), primary antibody incubation was performed over night.

    Techniques: Standard Deviation

    Left panels: representative healthy retina; right panels: representative ERU case. Differential interference contrast images of healthy (A) and ERU affected (B) retinal specimen demonstrating that in ERU state, normal retinal architecture is disturbed. GRP78 (green color), a marker staining retinal ganglion cells and a population of inner nuclear layer cells in equine retina was equally expressed in physiological (C) and ERU state (D). Synaptotagmin-1 (Syt1, red color) signal in healthy retina (E) was most prominent in retinal ganglion cell somata, their axons in the nerve fiber layer and in somata of a cell population in the inner nuclear layer, with additional staining foci in the outer and inner plexiform layer and photoreceptor outer segments, while ERU affected retinal sections (F), presented with a clearly reduced overall Syt1 signal. Overlay of GRP78 and Syt1 signals (G: healthy, H: ERU) indicated that in the ERU affected section, Syt1 expression is reduced, although structures expressing it in physiological state are still present. Cell nuclei were counterstained with DAPI (blue color).

    Journal: PLoS ONE

    Article Title: Retinal Glycoprotein Enrichment by Concanavalin A Enabled Identification of Novel Membrane Autoantigen Synaptotagmin-1 in Equine Recurrent Uveitis

    doi: 10.1371/journal.pone.0050929

    Figure Lengend Snippet: Left panels: representative healthy retina; right panels: representative ERU case. Differential interference contrast images of healthy (A) and ERU affected (B) retinal specimen demonstrating that in ERU state, normal retinal architecture is disturbed. GRP78 (green color), a marker staining retinal ganglion cells and a population of inner nuclear layer cells in equine retina was equally expressed in physiological (C) and ERU state (D). Synaptotagmin-1 (Syt1, red color) signal in healthy retina (E) was most prominent in retinal ganglion cell somata, their axons in the nerve fiber layer and in somata of a cell population in the inner nuclear layer, with additional staining foci in the outer and inner plexiform layer and photoreceptor outer segments, while ERU affected retinal sections (F), presented with a clearly reduced overall Syt1 signal. Overlay of GRP78 and Syt1 signals (G: healthy, H: ERU) indicated that in the ERU affected section, Syt1 expression is reduced, although structures expressing it in physiological state are still present. Cell nuclei were counterstained with DAPI (blue color).

    Article Snippet: Using polyclonal rabbit anti-synaptotagmin-1 (dilution 1∶1500; Abcam) and monoclonal mouse anti Glucose-regulated protein 78 (GRP78) antibody (dilution 1∶50, BD Biosciences, Heidelberg, Germany), primary antibody incubation was performed over night.

    Techniques: Marker, Staining, Expressing

    Figure 2A: Anti-synaptotagmin-1 positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.

    Journal: PLoS ONE

    Article Title: Retinal Glycoprotein Enrichment by Concanavalin A Enabled Identification of Novel Membrane Autoantigen Synaptotagmin-1 in Equine Recurrent Uveitis

    doi: 10.1371/journal.pone.0050929

    Figure Lengend Snippet: Figure 2A: Anti-synaptotagmin-1 positive IgG in healthy sera (left, white dots) and ERU cases (right, black dots). Each dot represents the absorbance value of an individual serum sample, measured at 450 nm). The Cut-off value was set at the mean value of negative controls plus the 10-fold standard deviation, represented by the horizontal separation line. In ERU samples, anti-Syt1-autoantibodies are more frequently present than in healthy samples (* = p≤0.05). Figure 2B: Comparison of positive reactions (black) and negative reactions (white) in control sera (left bar) and ERU samples (right bar) expressed in per cent. While in controls, the prevalence of positive IgG reactions to Syt1 was 37%, prevalence in the ERU group was 56%. Prevalence in percent was calculated by dividing numbers of positive samples by numbers of tested samples in each group.

    Article Snippet: For detection of Syt1, blots were incubated with polyclonal rabbit anti-synaptotagmin-1 antibody (dilution 1∶1000, Abcam, Berlin, Germany) followed by goat anti rabbit IgG POD (dilution 1∶3000, Sigma-Aldrich).

    Techniques: Standard Deviation

    Left panels: representative healthy retina; right panels: representative ERU case. Differential interference contrast images of healthy (A) and ERU affected (B) retinal specimen demonstrating that in ERU state, normal retinal architecture is disturbed. GRP78 (green color), a marker staining retinal ganglion cells and a population of inner nuclear layer cells in equine retina was equally expressed in physiological (C) and ERU state (D). Synaptotagmin-1 (Syt1, red color) signal in healthy retina (E) was most prominent in retinal ganglion cell somata, their axons in the nerve fiber layer and in somata of a cell population in the inner nuclear layer, with additional staining foci in the outer and inner plexiform layer and photoreceptor outer segments, while ERU affected retinal sections (F), presented with a clearly reduced overall Syt1 signal. Overlay of GRP78 and Syt1 signals (G: healthy, H: ERU) indicated that in the ERU affected section, Syt1 expression is reduced, although structures expressing it in physiological state are still present. Cell nuclei were counterstained with DAPI (blue color).

    Journal: PLoS ONE

    Article Title: Retinal Glycoprotein Enrichment by Concanavalin A Enabled Identification of Novel Membrane Autoantigen Synaptotagmin-1 in Equine Recurrent Uveitis

    doi: 10.1371/journal.pone.0050929

    Figure Lengend Snippet: Left panels: representative healthy retina; right panels: representative ERU case. Differential interference contrast images of healthy (A) and ERU affected (B) retinal specimen demonstrating that in ERU state, normal retinal architecture is disturbed. GRP78 (green color), a marker staining retinal ganglion cells and a population of inner nuclear layer cells in equine retina was equally expressed in physiological (C) and ERU state (D). Synaptotagmin-1 (Syt1, red color) signal in healthy retina (E) was most prominent in retinal ganglion cell somata, their axons in the nerve fiber layer and in somata of a cell population in the inner nuclear layer, with additional staining foci in the outer and inner plexiform layer and photoreceptor outer segments, while ERU affected retinal sections (F), presented with a clearly reduced overall Syt1 signal. Overlay of GRP78 and Syt1 signals (G: healthy, H: ERU) indicated that in the ERU affected section, Syt1 expression is reduced, although structures expressing it in physiological state are still present. Cell nuclei were counterstained with DAPI (blue color).

    Article Snippet: For detection of Syt1, blots were incubated with polyclonal rabbit anti-synaptotagmin-1 antibody (dilution 1∶1000, Abcam, Berlin, Germany) followed by goat anti rabbit IgG POD (dilution 1∶3000, Sigma-Aldrich).

    Techniques: Marker, Staining, Expressing

    (a) neuronal maturation markers of cultured mouse cortical neurons (DIV7) ( top ) and hIPSC-derived neurons ( bottom ). Bright-field and merged fluorescence images of neuronal markers, β-tubulin III (TUBB3) and Map2 (MAP2), and synaptic proteins, postsynaptic density protein 95 (PSD95) and synaptotagmin I (SYT1), with DAPI (nuclei). Scale bar = 50 µm. Visualized dendritic branches and the presence of synaptic markers are characteristic of functional mature neurons. Scale bar = 50 µm. (b) Principal component analysis visualization of chromatin accessibility changes in hIPSC-induced neurons. (c) Number of DA-peaks recovered for each time point and stimulation category when compared versus control hIPSC-derived neurons. Gained (new) and closing (new) indicate peaks observed as differentially accessible for the first time in that time point, whereas gained and closing indicate DA-peaks already observed as DA in a previous time point. (d) Hierarchical clustering of normalized counts obtained from ATAC-seq consensus peaks ( left ) and only DA-peaks ( right ).

    Journal: bioRxiv

    Article Title: Comparative chromatin accessibility upon BDNF-induced neuronal activity delineates neuronal regulatory elements

    doi: 10.1101/2021.05.28.446128

    Figure Lengend Snippet: (a) neuronal maturation markers of cultured mouse cortical neurons (DIV7) ( top ) and hIPSC-derived neurons ( bottom ). Bright-field and merged fluorescence images of neuronal markers, β-tubulin III (TUBB3) and Map2 (MAP2), and synaptic proteins, postsynaptic density protein 95 (PSD95) and synaptotagmin I (SYT1), with DAPI (nuclei). Scale bar = 50 µm. Visualized dendritic branches and the presence of synaptic markers are characteristic of functional mature neurons. Scale bar = 50 µm. (b) Principal component analysis visualization of chromatin accessibility changes in hIPSC-induced neurons. (c) Number of DA-peaks recovered for each time point and stimulation category when compared versus control hIPSC-derived neurons. Gained (new) and closing (new) indicate peaks observed as differentially accessible for the first time in that time point, whereas gained and closing indicate DA-peaks already observed as DA in a previous time point. (d) Hierarchical clustering of normalized counts obtained from ATAC-seq consensus peaks ( left ) and only DA-peaks ( right ).

    Article Snippet: Primary antibodies (mouse monoclonal anti-Map2 (Sigma, m9942), rabbit polyclonal anti-Synaptotagmin (Cell Signaling Technologies, 3347), mouse monoclonal anti-beta III Tubulin (Abcam, ab78078) and mouse monoclonal anti-PSD95 (Thermofisher, MA1-046)) were diluted 1:200 in 0.5% BSA (with the exception of Synaptotagmin, 1:50) and incubated for 1h at room temperature, followed by washes with PBS before the addition of the secondary antibody (goat anti-rabbit or mouse IgG conjugated to Alexa Fluor 594, (Life Technologies, A11012 and A11005), diluted 1:500 in 0.5% BSA, for 30 minutes at room temperature.

    Techniques: Cell Culture, Derivative Assay, Fluorescence, Functional Assay

    Journal: Cell systems

    Article Title: Pulse-chase proteomics of the App Knock-In mouse models of Alzheimer’s disease reveals synaptic dysfunction originates in presynaptic terminals

    doi: 10.1016/j.cels.2020.11.007

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: The following primary antibodies were used for Western and dot blots: Anti-Human Amyloid-beta (N) (82E1) (also detects β-CTF) mouse monoclonal at 1:1,000 (Immuno-Biological Laboratories Cat# 10323, RRID: AB_10707424); Anti-Amyloid beta precursor protein [Y188] rabbit monoclonal at 1:1,000 (Abcam Cat# ab32136, RRID: AB_2289606); Anti-AP180 (Snap91) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 155 003, RRID: AB_887691); Anti-Gapdh (0411) mouse monoclonal at 1:2,000 (Santa Cruz Biotechnology Cat# sc-47724, RRID: AB_627678); Anti-Pip5k1c rabbit polyclonal at 1:500 (Novus Cat# NBP1–82986, RRID: AB_11029240); Anti-Snap25 rabbit polyclonal at 1:500 (Synaptic Systems Cat# 111 002, RRID: AB_887790); Anti-Synaptobrevin 2 (Vamp2) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 104 202, RRID: AB_887810); Anti-Synaptophysin mouse monoclonal at 1:1,1000 (Sigma-Aldrich Cat# S5768, RRID: AB_477523); Anti-Synaptotagmin 1/2 cytoplasmic tail rabbit polyclonal at 1:500 (Synaptic Systems Cat# 105 003AF, RRID: AB_2744565); Anti-Syntaxin 1B rabbit polyclonal at 1:500 (Synaptic Systems Cat# 110 402, RRID: AB_887901); Anti-Vamp1 rabbit polyclonal at 1:500 (Abcam Cat# ab41324, RRID: AB_1281203); Anti-Ubiquitin P4D1 mouse monoclonal at 1:1,000 (Santa Cruz Biotechnology Cat# sc-8017, RRID: AB_628423); Anti-VCP mouse monoclonal at 1:2,000 (Abcam Cat# ab11433, RRID: AB_298039).

    Techniques: Recombinant, Sequencing, Modification, Blocking Assay, Bicinchoninic Acid Protein Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Mass Spectrometry