rabbit polyclonal antibody against phospho erbb2  (Cell Signaling Technology Inc)

Journal: The Journal of cell biology

Article Title: EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis.

doi: 10.1083/jcb.202012073

Figure Lengend Snippet: Figure 4. Age-associated decline of EGFR signaling in wound healing. (A) Schematic representation of wound healing experiments with young and aged mice. Details are described in Materials and methods. (B) Left: Western blots of EGFR, phosphorylated EGFR (pEGFR), ErbB2, and phosphorylated ErbB2 (pErbB2). Middle and right: Quantification of EGFR and pEGFR expression normalized by GAPDH and EGFR, respectively. (C) Immunostaining with anti-EGFR and anti-pEGFR in infant and aged human keratinocytes with or without EGF treatment. Scale bar, 20 µm. (D) EGF-induced colony expansion of infant and aged human keratinocytes. White dotted lines indicate the periphery of keratinocyte colonies. Scale bar, 200 µm. (E) Relative increase in area of expanding colonies of keratinocytes isolated from infants and aged patients after the addition of EGF. (F) Distribution of colony types after 6 d of cultivation with or without EGF in cultures of infant and aged keratinocytes. The ages of patients from whom keratinocytes were isolated are indicated in each graph. P values were calculated with the two-tailed Student’s t test

Article Snippet: Primary antibodies used for Western blotting were as follows: rabbit monoclonal antibody against EGFR (Abcam; ab52894, 1:1,000), rabbit polyclonal antibody against phospho-EGFR (Tyr845; Millipore; 07-820, 1:1,000), rabbit polyclonal antibody against ErbB2 (Cell Signaling Technology; 2165, 1:1,000), rabbit polyclonal antibody against phospho-ErbB2 (Tyr1248; Cell Signaling Technology; 2247, 1:1,000), mouse monoclonal antibody against COL17A1 (Covance; SIG-3780, 1:500), mouse monoclonal antibody against PLEC (Cosmo Bio; NU-01-PLC, 1:200), rabbit polyclonal antibody against ITGA6 (Abcam; ab97760, 1:1,000), rabbit polyclonal antibody against ITGB4 (Santa Cruz Biotechnology; sc-9090, 1:1,000), rabbit monoclonal antibody against ITGB1 (Abcam; ab179471, 1:1,000), rabbit polyclonal antibody against GAPDH (Cell Signaling Technology; 2118, 1:5,000), and mouse monoclonal antibody against TIMP1 (Thermo Fisher Scientific; MS-608, 1:250).

Techniques: Western Blot, Expressing, Immunostaining, Isolation, Two Tailed Test

Journal: Cell metabolism

Article Title: Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway

doi: 10.1016/j.cmet.2018.08.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-phospho-HER2/ErbB2 (Tyr1248) antibody , Cell Signaling Technology , 2247S, RRID:AB_331725.

Techniques: Derivative Assay, Recombinant, FLAG-tag, Plasmid Preparation, shRNA, Software, Cloning, Mutagenesis, SYBR Green Assay, CyQUANT Assay, Proliferation Assay, Cell Viability Assay, Protease Inhibitor

Journal: Cell chemical biology

Article Title: Mechanism of allosteric coupling into and through the plasma membrane by EGFR

doi: 10.1016/j.chembiol.2018.04.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Phospho-Her2/ErbB2 Tyr 1248 , Cell Signaling Technology , Cat#2247; RRID: AB_331725.

Techniques: Recombinant, Saline, Protease Inhibitor, Binding Assay, Western Blot, Mutagenesis, Fluorescence, Live Cell Imaging, Software, Membrane

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Traditional Chinese Medicine Tang-Luo-Ning Ameliorates Sciatic Nerve Injuries in Streptozotocin-Induced Diabetic Rats

doi: 10.1155/2013/989670

Figure Lengend Snippet: Effect of TLN and α -LA on Nrg1/ErbB2 downstream signal pathway in sciatic nerve of streptozotocin-induced rats. A representative western blot ((a), (c), (e), and (g)) and the grey value analyses of protein expression ((b), (d), (f), and (h)) in sciatic nerves from different experimental groups. ((a), (b)) Ratio of P-ErbB2 to T-ErbB2 (P/T); ((c), (d)) ratio of P-Erk to T-Erk (P/T); ((e), (f)) ratio of P-Bad (Ser112) to T-Bad (P/T); ((g), (h)) ratio of P-Akt to T-Akt (P/T). Statistical analysis was performed using the ANOVA procedure t test (LSD). Data are expressed as mean ± SD. # P < 0.05 versus CTL group, * P < 0.05 versus STZ group. n = 4 per group. The protein expression of ratio of ErbB2 Erk, Bad (P/T) decreased compared with CTL group ( P < 0.05), while Tang-Luo-Ning and alpha-lipoic acid intervention could increase the ratio above compared with STZ group ( P < 0.05). There were no significant differences of ratio of Akt (P/T) between the four groups. Four groups: CTL group: nonstreptozotocin-induced group, STZ group: streptozotocin-induced diabetic group, TLN group: Tang-Luo-Ning group, and α -LA group: alpha-lipoic acid group. ANOVA: analysis of variance; LSD- t test: least significant difference t test.

Article Snippet: Rabbit polyclonal anti-ErbB2 antibody (dilution: 1 : 4000, Novus, Biologicals, Inc.; Littleton, CO, USA), Rabbit polyclonal anti-Phospho-ErbB2 (Tyr1248) antibody (dilution: 1 : 3000, Novus, Biologicals, Inc.; Littleton, CO, USA), after washing 3 × 10 min with Tris-buffered saline, horseradish peroxidase conjugated secondary antibody (dilution 1 : 1000: P-Bad ser112/ser136, 1 : 2000: other antibodies) was added and incubated for 1 hr with shaking.

Techniques: Western Blot, Expressing