rabbit polyclonal anti cdk1 t161p  (Cell Signaling Technology Inc)

Journal: Developmental cell

Article Title: Low level saturated fatty acid palmitate benefits liver cells by boosting mitochondrial metabolism via CDK1-SIRT3-CPT2 cascade

doi: 10.1016/j.devcel.2019.11.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Phospho-Cdc2 (Thr161) , Cell Signaling , Cat# 9114; RRID: AB_2260040.

Techniques: Virus, Recombinant, Red Blood Cell Lysis, Luciferase, Protease Inhibitor, Magnetic Beads, Plasmid Preparation, Avidin-Biotin Assay, Blocking Assay, Mutagenesis, AST Assay, Software, Immunofluorescence, CRISPR, Phospho-proteomics

Journal: Reproduction (Cambridge, England)

Article Title: Fertilization differently affects the levels of cyclin B1 and M-phase promoting factor activity in maturing and metaphase II mouse oocytes.

doi: 10.1530/REP-08-0271

Figure Lengend Snippet: Figure 5 Phosphorylation ofCDC2A in maturing mouse oocytes fertilized in a polyspermic manner or treated with diamide. Western blot analysis of the amount of CDC2A phosphorylated on Thr161 (CDC2A-pThr161), total CDC2Aanda-tubulininmaturingoocytesfertilizedinapolyspermic manner (A) or treated with diamide (B) and their control counterparts. (C) CDC2A phosphorylated on Tyr15 (CDC2A-pTyr15) and total CDC2A in maturing oocytes and embryo lysate: lane 1, control oocytes, untreated with diamide; 2, oocytes treated with diamide; 3, control, unfertilized oocytes; 4, oocytes fertilized by three to nine spermatozoa; 5, lysate prepared from 13.5-day-old mouse embryos (w5 mg total protein); 6, GV oocytes; 7, metaphase II oocytes. The same membranes were used to detect all examined proteins.

Article Snippet: PVDF membranes were probed overnight at 4 8C with: 1) a mouse monoclonal antibody (MAB) against total CDC2A (diluted 1:100, Abcam, Cambridge, UK, cat. no. ab18); 2) a rabbit polyclonal antibody anti-CDC2A phosphorylated at pTyr15 (anti-CDC2A-pTyr15, 1:100, Abcam, cat. no. ab10533); 3) a rabbit polyclonal antibody against CDC2A phosphorylated at Thr161 (anti-CDC2A-pThr161, 1:50, Cell Signaling Technology, Danvers, MA, USA, cat. no. 9114); 4) a mouse MAB against a-tubulin (1:1000, Sigma–Aldrich, cat. no. T5168).

Techniques: Phospho-proteomics, Western Blot, Control