Journal: Physiological Research

Article Title: Genistein does not Inhibit TGF-β1-Induced Conversion of Human Dermal Fibroblasts to Myofibroblasts

doi: 10.33549/physiolres.934666

Figure Lengend Snippet: Antibodies used for Western blot and immunofluorescence.

Article Snippet: The list of antibodies applied in Western blotting is shown in . table ft1 table-wrap mode="anchored" t5 caption a7 Antibodies used for Western blot Primary Antibody Abbreviation Host Isotype Clonality Produced by α-Smooth muscle actin α-SMA Rabbit IgG Monoclonal CST, Danvers, MA, USA Fibronectin Fibr Rabbit IgG Monoclonal Abcam, Cambridge, UK Phospho-ERK1/2 pERK1/2 Rabbit Polyclonal CST, Danvers, MA, USA Phospho-AKT pAKT Rabbit IgG Monoclonal CST, Danvers, MA, USA Phospho-SMAD3 pSMAD3 Rabbit IgG Monoclonal Abcam, Cambridge, UK ERK1/2 ERK1/2 Rabbit IgG Monoclonal CST, Danvers, MA, USA SMAD3 SMAD3 Rabbit IgG Monoclonal CST, Danvers, MA, USA AKT AKT Rabbit Polyclonal CST, Danvers, MA, USA β-Actin β-actin Rabbit IgG Monoclonal CST, Danvers, MA, USA D-Glyceraldehyde 3-phosphate GAPDH Rabbit IgG Monoclonal CST, Danvers, MA, USA Secondary Antibody Host Isotype Produced by Anti-rabbit, HRP-linked goat IgG CST, Danvers, MA, USA Antibodies used for immunofluorescence Primary Antibody Abbreviation Host Produced by Secondary Antibody Produced by Channel α-Smooth muscle actin α-SMA Mouse monoclonal DakoCytomation, Denmark Goat anti-mouse Sigma-Aldrich, St. Louis, MO, USA TRITC-red Fibronectin Fibr Rabbit polyclonal DakoCytomation, Denmark Goat anti-rabbit Sigma-Aldrich, St. Louis, MO, USA FITC-green Open in a separate window Antibodies used for Western blot and immunofluorescence.

Techniques: Western Blot, Immunofluorescence, Produced