rabbit polyclonal anti nav1 8  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti nav1 8
    Immunochemical study of <t>Nav1.8,</t> Nav1.5, PGP 9.5, anti‐choline acetyltransferase (Ch AT ), and anti‐tyrosine hydroxylase (TH) in partial dissected intracardiac ganglia. Protein expression of Nav1.8 (A and B), Nav1.5 (C and D), PGP 9.5 (E and F), Ch AT (G and H), and TH (I and J) present in intracardiac ganglia. Images on the left side show fluorescein isothiocyanate green fluorescence. Images on the right side show nonstained components of the ganglia. Calibration bars in (A through D) are 20 μm and in (E through J) are 50 μm.
    Rabbit Polyclonal Anti Nav1 8, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti nav1 8/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti nav1 8 - by Bioz Stars, 2022-01
    86/100 stars

    Images

    1) Product Images from "Neuronal Nav1.8 Channels as a Novel Therapeutic Target of Acute Atrial Fibrillation Prevention"

    Article Title: Neuronal Nav1.8 Channels as a Novel Therapeutic Target of Acute Atrial Fibrillation Prevention

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.116.004050

    Immunochemical study of Nav1.8, Nav1.5, PGP 9.5, anti‐choline acetyltransferase (Ch AT ), and anti‐tyrosine hydroxylase (TH) in partial dissected intracardiac ganglia. Protein expression of Nav1.8 (A and B), Nav1.5 (C and D), PGP 9.5 (E and F), Ch AT (G and H), and TH (I and J) present in intracardiac ganglia. Images on the left side show fluorescein isothiocyanate green fluorescence. Images on the right side show nonstained components of the ganglia. Calibration bars in (A through D) are 20 μm and in (E through J) are 50 μm.
    Figure Legend Snippet: Immunochemical study of Nav1.8, Nav1.5, PGP 9.5, anti‐choline acetyltransferase (Ch AT ), and anti‐tyrosine hydroxylase (TH) in partial dissected intracardiac ganglia. Protein expression of Nav1.8 (A and B), Nav1.5 (C and D), PGP 9.5 (E and F), Ch AT (G and H), and TH (I and J) present in intracardiac ganglia. Images on the left side show fluorescein isothiocyanate green fluorescence. Images on the right side show nonstained components of the ganglia. Calibration bars in (A through D) are 20 μm and in (E through J) are 50 μm.

    Techniques Used: Expressing, Fluorescence

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  • 86
    Alomone Labs rabbit polyclonal anti nav1 8
    Immunochemical study of <t>Nav1.8,</t> Nav1.5, PGP 9.5, anti‐choline acetyltransferase (Ch AT ), and anti‐tyrosine hydroxylase (TH) in partial dissected intracardiac ganglia. Protein expression of Nav1.8 (A and B), Nav1.5 (C and D), PGP 9.5 (E and F), Ch AT (G and H), and TH (I and J) present in intracardiac ganglia. Images on the left side show fluorescein isothiocyanate green fluorescence. Images on the right side show nonstained components of the ganglia. Calibration bars in (A through D) are 20 μm and in (E through J) are 50 μm.
    Rabbit Polyclonal Anti Nav1 8, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti nav1 8/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti nav1 8 - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

    93
    Alomone Labs anti rabbit nav1 8 antibody
    Loss of the clock gene Bmal1 in <t>Nav1.8+</t> sensory neurons leads to reduced OA (osteoarthritis) pain. (Fig. 4A) Mechanical hyperalgesia was measured using von Frey filament test. The paw withdrawal threshold (PWT) of the bmal1f/f Nav1.8CreERT(−) group (n = 5) were low starting at 2 weeks after OA induction, and were lower than bmal1f/f Nav1.8CreERT(+) group (n = 5) at all time points. (Fig. 4B) Heat hyperalgesia was measured using hot-plate test. The paw thermal threshold (PTT) in the bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) group was not significantly changed. However, expression of Rev-Erb-α in the dorsal root ganglia (DRG) following OA surgery was higher and TRPV1 was lower in in bmal1f/f Nav1.8CreERT(+) vs. bmal1f/f Nav1.8CreERT(−) mice. Double immunofluorescence staining of Rev-Erb-α (red; Fig. 4C and Fig. 4F) and TRPV1 (green; Fig. 4D Fig. 4G) in DRGs of bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appears yellow (Fig. Fig. 4E Fig. 4H). A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of Rev-Erb-α and TRPV1 expression in DRG (Fig. 4I-4J). Values are mean±SEM.
    Anti Rabbit Nav1 8 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit nav1 8 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rabbit nav1 8 antibody - by Bioz Stars, 2022-01
    93/100 stars
      Buy from Supplier

    Image Search Results


    Immunochemical study of Nav1.8, Nav1.5, PGP 9.5, anti‐choline acetyltransferase (Ch AT ), and anti‐tyrosine hydroxylase (TH) in partial dissected intracardiac ganglia. Protein expression of Nav1.8 (A and B), Nav1.5 (C and D), PGP 9.5 (E and F), Ch AT (G and H), and TH (I and J) present in intracardiac ganglia. Images on the left side show fluorescein isothiocyanate green fluorescence. Images on the right side show nonstained components of the ganglia. Calibration bars in (A through D) are 20 μm and in (E through J) are 50 μm.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Neuronal Nav1.8 Channels as a Novel Therapeutic Target of Acute Atrial Fibrillation Prevention

    doi: 10.1161/JAHA.116.004050

    Figure Lengend Snippet: Immunochemical study of Nav1.8, Nav1.5, PGP 9.5, anti‐choline acetyltransferase (Ch AT ), and anti‐tyrosine hydroxylase (TH) in partial dissected intracardiac ganglia. Protein expression of Nav1.8 (A and B), Nav1.5 (C and D), PGP 9.5 (E and F), Ch AT (G and H), and TH (I and J) present in intracardiac ganglia. Images on the left side show fluorescein isothiocyanate green fluorescence. Images on the right side show nonstained components of the ganglia. Calibration bars in (A through D) are 20 μm and in (E through J) are 50 μm.

    Article Snippet: Tissue sections were incubated at 4°C overnight with primary antibody specific for rabbit polyclonal anti‐Nav1.8 (Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti‐Nav1.5 (Alomone Labs, Jerusalem, Israel), goat polyclonal anti‐PGP9.5 (Abcam, Cambridge, MA), goat polyclonal anti‐choline acetyltransferase (ChAT, CHEMICON International, Temecula, Canada), and mouse monoclonal anti‐tyrosine hydroxylase (TH, Alpha Diagnostic International, San Antonio, TX).

    Techniques: Expressing, Fluorescence

    Loss of the clock gene Bmal1 in Nav1.8+ sensory neurons leads to reduced OA (osteoarthritis) pain. (Fig. 4A) Mechanical hyperalgesia was measured using von Frey filament test. The paw withdrawal threshold (PWT) of the bmal1f/f Nav1.8CreERT(−) group (n = 5) were low starting at 2 weeks after OA induction, and were lower than bmal1f/f Nav1.8CreERT(+) group (n = 5) at all time points. (Fig. 4B) Heat hyperalgesia was measured using hot-plate test. The paw thermal threshold (PTT) in the bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) group was not significantly changed. However, expression of Rev-Erb-α in the dorsal root ganglia (DRG) following OA surgery was higher and TRPV1 was lower in in bmal1f/f Nav1.8CreERT(+) vs. bmal1f/f Nav1.8CreERT(−) mice. Double immunofluorescence staining of Rev-Erb-α (red; Fig. 4C and Fig. 4F) and TRPV1 (green; Fig. 4D Fig. 4G) in DRGs of bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appears yellow (Fig. Fig. 4E Fig. 4H). A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of Rev-Erb-α and TRPV1 expression in DRG (Fig. 4I-4J). Values are mean±SEM.

    Journal: Gene

    Article Title: Pharmacological Targeting of the Mammalian Clock Reveals a Novel Analgesic for Osteoarthritis-Induced Pain

    doi: 10.1016/j.gene.2018.02.048

    Figure Lengend Snippet: Loss of the clock gene Bmal1 in Nav1.8+ sensory neurons leads to reduced OA (osteoarthritis) pain. (Fig. 4A) Mechanical hyperalgesia was measured using von Frey filament test. The paw withdrawal threshold (PWT) of the bmal1f/f Nav1.8CreERT(−) group (n = 5) were low starting at 2 weeks after OA induction, and were lower than bmal1f/f Nav1.8CreERT(+) group (n = 5) at all time points. (Fig. 4B) Heat hyperalgesia was measured using hot-plate test. The paw thermal threshold (PTT) in the bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) group was not significantly changed. However, expression of Rev-Erb-α in the dorsal root ganglia (DRG) following OA surgery was higher and TRPV1 was lower in in bmal1f/f Nav1.8CreERT(+) vs. bmal1f/f Nav1.8CreERT(−) mice. Double immunofluorescence staining of Rev-Erb-α (red; Fig. 4C and Fig. 4F) and TRPV1 (green; Fig. 4D Fig. 4G) in DRGs of bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appears yellow (Fig. Fig. 4E Fig. 4H). A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of Rev-Erb-α and TRPV1 expression in DRG (Fig. 4I-4J). Values are mean±SEM.

    Article Snippet: After that, the DRG sections were incubated overnight with anti-rabbit TrkA antibody (1:200, Ab76291; Abcam), anti-rabbit NGF antibody (1:200; Sc-548; Santa Cruz Biotechnology), anti-rabbit Substance-P antibody (1:200; AB1566; Millipore Sigma), anti-rabbit CGRP antibody (1:500; ; Abcam), anti-rabbit Nav1.7 antibody (1:200; ASC-008; Alamone labs), anti-rabbit Nav1.8 antibody (1:200; ASC-016; Alamone labs), anti-guinea pig TRPV1 antibody (1:200; AB5566; Millipore), anti-mouse NeuN antibody (1:500; MAB377; Millipore), anti-rabbit Bmal1 antibody (1:100; NB1002288; Novus) and anti-mouse Rev-erb-α antibody (1:50,Sc-100910; Santa Cruz Biotechnology).

    Techniques: Hot Plate Test, Expressing, Mouse Assay, Double Immunofluorescence Staining

    Loss of the clock gene bmal1 in Nav1.8+ sensory neurons leads to reduced BMAL1 protein in DRG. Expression of BMAL1 protein targets in the dorsal root ganglia (DRG) following OA surgery in bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups. Double immunofluorescence staining of BMAL1 (green; 3A 3D) and NeuN (red; 3B 3E) in DRG of bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appears yellow (Fig. 3C 3F). A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of BMAL1 expression in DRGs shows lower expression in the bmal1f/f Nav1.8CreERT(+) group (Fig. 3G). Values are mean±SEM.

    Journal: Gene

    Article Title: Pharmacological Targeting of the Mammalian Clock Reveals a Novel Analgesic for Osteoarthritis-Induced Pain

    doi: 10.1016/j.gene.2018.02.048

    Figure Lengend Snippet: Loss of the clock gene bmal1 in Nav1.8+ sensory neurons leads to reduced BMAL1 protein in DRG. Expression of BMAL1 protein targets in the dorsal root ganglia (DRG) following OA surgery in bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups. Double immunofluorescence staining of BMAL1 (green; 3A 3D) and NeuN (red; 3B 3E) in DRG of bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appears yellow (Fig. 3C 3F). A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of BMAL1 expression in DRGs shows lower expression in the bmal1f/f Nav1.8CreERT(+) group (Fig. 3G). Values are mean±SEM.

    Article Snippet: After that, the DRG sections were incubated overnight with anti-rabbit TrkA antibody (1:200, Ab76291; Abcam), anti-rabbit NGF antibody (1:200; Sc-548; Santa Cruz Biotechnology), anti-rabbit Substance-P antibody (1:200; AB1566; Millipore Sigma), anti-rabbit CGRP antibody (1:500; ; Abcam), anti-rabbit Nav1.7 antibody (1:200; ASC-008; Alamone labs), anti-rabbit Nav1.8 antibody (1:200; ASC-016; Alamone labs), anti-guinea pig TRPV1 antibody (1:200; AB5566; Millipore), anti-mouse NeuN antibody (1:500; MAB377; Millipore), anti-rabbit Bmal1 antibody (1:100; NB1002288; Novus) and anti-mouse Rev-erb-α antibody (1:50,Sc-100910; Santa Cruz Biotechnology).

    Techniques: Expressing, Double Immunofluorescence Staining, Mouse Assay

    Loss of the clock gene Bmal1 in Nav1.8+ sensory neurons leads to reduced pain-related protein in DRG. Expression of pain-related targets in the dorsal root ganglia (DRG) following OA surgery in bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups. Double immunofluorescence staining of substance P (red; 5A-5B), NGF (red; 5C-5D), CGRP (red; 5E-5F), TrkA (red; 5G-5H) and Nav1.7 (red; 5I-5J) and NeuN (green) in DRG of bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appear yellow. A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of substance P, NGF, CGRP, TrkA and Nav1.7 expression in DRGs shows lower expression in the bmal1f/f Nav1.8CreERT(+) group (Fig. 5K-4O). Values are mean±SEM.

    Journal: Gene

    Article Title: Pharmacological Targeting of the Mammalian Clock Reveals a Novel Analgesic for Osteoarthritis-Induced Pain

    doi: 10.1016/j.gene.2018.02.048

    Figure Lengend Snippet: Loss of the clock gene Bmal1 in Nav1.8+ sensory neurons leads to reduced pain-related protein in DRG. Expression of pain-related targets in the dorsal root ganglia (DRG) following OA surgery in bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups. Double immunofluorescence staining of substance P (red; 5A-5B), NGF (red; 5C-5D), CGRP (red; 5E-5F), TrkA (red; 5G-5H) and Nav1.7 (red; 5I-5J) and NeuN (green) in DRG of bmal1f/f Nav1.8CreERT(−) bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appear yellow. A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of substance P, NGF, CGRP, TrkA and Nav1.7 expression in DRGs shows lower expression in the bmal1f/f Nav1.8CreERT(+) group (Fig. 5K-4O). Values are mean±SEM.

    Article Snippet: After that, the DRG sections were incubated overnight with anti-rabbit TrkA antibody (1:200, Ab76291; Abcam), anti-rabbit NGF antibody (1:200; Sc-548; Santa Cruz Biotechnology), anti-rabbit Substance-P antibody (1:200; AB1566; Millipore Sigma), anti-rabbit CGRP antibody (1:500; ; Abcam), anti-rabbit Nav1.7 antibody (1:200; ASC-008; Alamone labs), anti-rabbit Nav1.8 antibody (1:200; ASC-016; Alamone labs), anti-guinea pig TRPV1 antibody (1:200; AB5566; Millipore), anti-mouse NeuN antibody (1:500; MAB377; Millipore), anti-rabbit Bmal1 antibody (1:100; NB1002288; Novus) and anti-mouse Rev-erb-α antibody (1:50,Sc-100910; Santa Cruz Biotechnology).

    Techniques: Expressing, Double Immunofluorescence Staining, Mouse Assay

    Analgesic activity of the i.a. REV-ERB agonist SR9009 in a model of OA (osteoarthritis) induced pain. Expression of pain-related targets in the dorsal root ganglia (DRG) following groups of mice including naïve, PMM and SR9009 treated mice. Double immunofluorescence staining of TrkA (red; 7A-7C), NGF (red; 7D-7F), Nav1.7 (red; 7G-7I), Nav1.8 (red; 7J-7L) and TRPV1 (red; 7M-7O) and NeuN (green) in DRGs of Naïve, PMM and SR9009 treated groups mice. Co-localization of the two stains appears yellow. A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of TrkA, NGF, Nav1.7, Nav1.8 and TRPV1expression in DRGs (Fig. 7P-7T) showed that they were increased in OA mice, but that was reduced with i.a. SR9009. Values are mean±SEM.

    Journal: Gene

    Article Title: Pharmacological Targeting of the Mammalian Clock Reveals a Novel Analgesic for Osteoarthritis-Induced Pain

    doi: 10.1016/j.gene.2018.02.048

    Figure Lengend Snippet: Analgesic activity of the i.a. REV-ERB agonist SR9009 in a model of OA (osteoarthritis) induced pain. Expression of pain-related targets in the dorsal root ganglia (DRG) following groups of mice including naïve, PMM and SR9009 treated mice. Double immunofluorescence staining of TrkA (red; 7A-7C), NGF (red; 7D-7F), Nav1.7 (red; 7G-7I), Nav1.8 (red; 7J-7L) and TRPV1 (red; 7M-7O) and NeuN (green) in DRGs of Naïve, PMM and SR9009 treated groups mice. Co-localization of the two stains appears yellow. A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of TrkA, NGF, Nav1.7, Nav1.8 and TRPV1expression in DRGs (Fig. 7P-7T) showed that they were increased in OA mice, but that was reduced with i.a. SR9009. Values are mean±SEM.

    Article Snippet: After that, the DRG sections were incubated overnight with anti-rabbit TrkA antibody (1:200, Ab76291; Abcam), anti-rabbit NGF antibody (1:200; Sc-548; Santa Cruz Biotechnology), anti-rabbit Substance-P antibody (1:200; AB1566; Millipore Sigma), anti-rabbit CGRP antibody (1:500; ; Abcam), anti-rabbit Nav1.7 antibody (1:200; ASC-008; Alamone labs), anti-rabbit Nav1.8 antibody (1:200; ASC-016; Alamone labs), anti-guinea pig TRPV1 antibody (1:200; AB5566; Millipore), anti-mouse NeuN antibody (1:500; MAB377; Millipore), anti-rabbit Bmal1 antibody (1:100; NB1002288; Novus) and anti-mouse Rev-erb-α antibody (1:50,Sc-100910; Santa Cruz Biotechnology).

    Techniques: Activity Assay, Expressing, Mouse Assay, Double Immunofluorescence Staining

    ALA downregulated NaV1.7 and NaV1.8 expression. Notes: (A) Western blots for NaV1.7 of T13-L2 DRGs from NS- and ALA-treatment rats. Bar graph showed mean density relative to GAPHD for NaV1.7. ALA treatment greatly reduced expressions of NaV1.7 (n=4 for each group, ** p

    Journal: Journal of Pain Research

    Article Title: α-lipoic acid suppresses neuronal excitability and attenuates colonic hypersensitivity to colorectal distention in diabetic rats

    doi: 10.2147/JPR.S135017

    Figure Lengend Snippet: ALA downregulated NaV1.7 and NaV1.8 expression. Notes: (A) Western blots for NaV1.7 of T13-L2 DRGs from NS- and ALA-treatment rats. Bar graph showed mean density relative to GAPHD for NaV1.7. ALA treatment greatly reduced expressions of NaV1.7 (n=4 for each group, ** p

    Article Snippet: The primary antibodies used to probe the target proteins included rabbit anti-NaV1.7 or anti-NaV1.8 (1:200, Alomone Labs, Jerusalem, Israel), rabbit anti-GAPDH (1:1000, Biotechnology Co., CHN), and mouse anti-actin (1:1000; Chemicon, Temecula, CA, USA).

    Techniques: Expressing, Western Blot

    GDF-15 reduces Nav1.8 currents via ALK2 and intracellular signaling of PKA and ERK in small-diameter DRG neurons. A–C. The ALK2-specific inhibitor DMH-1 blocks GDF-15-induced inhibition of Nav1.8 currents. DMH-1 per se does not affect Nav1.8 currents (** P

    Journal: Neuroscience Bulletin

    Article Title: Growth Differentiation Factor-15 Produces Analgesia by Inhibiting Tetrodotoxin-Resistant Nav1.8 Sodium Channel Activity in Rat Primary Sensory Neurons

    doi: 10.1007/s12264-021-00709-5

    Figure Lengend Snippet: GDF-15 reduces Nav1.8 currents via ALK2 and intracellular signaling of PKA and ERK in small-diameter DRG neurons. A–C. The ALK2-specific inhibitor DMH-1 blocks GDF-15-induced inhibition of Nav1.8 currents. DMH-1 per se does not affect Nav1.8 currents (** P

    Article Snippet: Sections were incubated in blocking solution (10% normal donkey serum in 0.01 mol/L PBS with 0.3% Triton X-100) for 2 h at RT, then overnight at 4°C with the following primary antibodies: goat anti-ALK2 (1:100, R & D Systems, AF637, Minneapolis, MN, USA), rabbit anti-Nav1.8 (1:200, ASC-016, Alomone, Jerusalem, Israel), rabbit anti-substance P (1:4000, Peninsula Labs, RIN7451, San Carlos, CA, USA), rabbit anti-CGRP, 1:20000, Peninsula Labs, IHC6006), mouse anti-peripherin (1:1000, Millipore, mab1527, Billerica, MA, USA).

    Techniques: Inhibition

    Schematic showing how GDF-15 modulates peripheral nociceptive information. GDF-15 inhibits Nav1.8 on nociceptors by membrane ALK2 and downstream intracellular signals, such as PKA and ERK, leading to a reduction in the excitability of DRG neurons and pain relief.

    Journal: Neuroscience Bulletin

    Article Title: Growth Differentiation Factor-15 Produces Analgesia by Inhibiting Tetrodotoxin-Resistant Nav1.8 Sodium Channel Activity in Rat Primary Sensory Neurons

    doi: 10.1007/s12264-021-00709-5

    Figure Lengend Snippet: Schematic showing how GDF-15 modulates peripheral nociceptive information. GDF-15 inhibits Nav1.8 on nociceptors by membrane ALK2 and downstream intracellular signals, such as PKA and ERK, leading to a reduction in the excitability of DRG neurons and pain relief.

    Article Snippet: Sections were incubated in blocking solution (10% normal donkey serum in 0.01 mol/L PBS with 0.3% Triton X-100) for 2 h at RT, then overnight at 4°C with the following primary antibodies: goat anti-ALK2 (1:100, R & D Systems, AF637, Minneapolis, MN, USA), rabbit anti-Nav1.8 (1:200, ASC-016, Alomone, Jerusalem, Israel), rabbit anti-substance P (1:4000, Peninsula Labs, RIN7451, San Carlos, CA, USA), rabbit anti-CGRP, 1:20000, Peninsula Labs, IHC6006), mouse anti-peripherin (1:1000, Millipore, mab1527, Billerica, MA, USA).

    Techniques:

    GDF-15 inhibits Nav1.8 currents in small-diameter DRG neurons. A. Isolation of TTX-resistant Nav1.8 currents in the presence of 500 nmol/L TTX. Cells were depolarized to a variety of potentials (–50 mV to +40 mV) from a holding potential of –60 mV, to elicit Nav1.8 currents. B. I–V curve of Nav1.8 currents. C and D. Nav1.8 currents are reduced following incubation with GDF-15 for 10, 20, and 30 min (* P

    Journal: Neuroscience Bulletin

    Article Title: Growth Differentiation Factor-15 Produces Analgesia by Inhibiting Tetrodotoxin-Resistant Nav1.8 Sodium Channel Activity in Rat Primary Sensory Neurons

    doi: 10.1007/s12264-021-00709-5

    Figure Lengend Snippet: GDF-15 inhibits Nav1.8 currents in small-diameter DRG neurons. A. Isolation of TTX-resistant Nav1.8 currents in the presence of 500 nmol/L TTX. Cells were depolarized to a variety of potentials (–50 mV to +40 mV) from a holding potential of –60 mV, to elicit Nav1.8 currents. B. I–V curve of Nav1.8 currents. C and D. Nav1.8 currents are reduced following incubation with GDF-15 for 10, 20, and 30 min (* P

    Article Snippet: Sections were incubated in blocking solution (10% normal donkey serum in 0.01 mol/L PBS with 0.3% Triton X-100) for 2 h at RT, then overnight at 4°C with the following primary antibodies: goat anti-ALK2 (1:100, R & D Systems, AF637, Minneapolis, MN, USA), rabbit anti-Nav1.8 (1:200, ASC-016, Alomone, Jerusalem, Israel), rabbit anti-substance P (1:4000, Peninsula Labs, RIN7451, San Carlos, CA, USA), rabbit anti-CGRP, 1:20000, Peninsula Labs, IHC6006), mouse anti-peripherin (1:1000, Millipore, mab1527, Billerica, MA, USA).

    Techniques: Isolation, Incubation

    ALK2 expression in DRG neurons. A–D. Double immunofluorescence reveals the expression of ALK2 in peripherin- ( A ), substance P- (SP, B ), calcitonin gene-related peptide- (CGRP, C ), and isolectin B4- (IB4, D ) positive neurons in the DRG (scale bars, 50 μm). E. Immunocytochemistry double staining of ALK2 and Nav1.8 in isolated DRG neurons (scale bar, 30 μm).

    Journal: Neuroscience Bulletin

    Article Title: Growth Differentiation Factor-15 Produces Analgesia by Inhibiting Tetrodotoxin-Resistant Nav1.8 Sodium Channel Activity in Rat Primary Sensory Neurons

    doi: 10.1007/s12264-021-00709-5

    Figure Lengend Snippet: ALK2 expression in DRG neurons. A–D. Double immunofluorescence reveals the expression of ALK2 in peripherin- ( A ), substance P- (SP, B ), calcitonin gene-related peptide- (CGRP, C ), and isolectin B4- (IB4, D ) positive neurons in the DRG (scale bars, 50 μm). E. Immunocytochemistry double staining of ALK2 and Nav1.8 in isolated DRG neurons (scale bar, 30 μm).

    Article Snippet: Sections were incubated in blocking solution (10% normal donkey serum in 0.01 mol/L PBS with 0.3% Triton X-100) for 2 h at RT, then overnight at 4°C with the following primary antibodies: goat anti-ALK2 (1:100, R & D Systems, AF637, Minneapolis, MN, USA), rabbit anti-Nav1.8 (1:200, ASC-016, Alomone, Jerusalem, Israel), rabbit anti-substance P (1:4000, Peninsula Labs, RIN7451, San Carlos, CA, USA), rabbit anti-CGRP, 1:20000, Peninsula Labs, IHC6006), mouse anti-peripherin (1:1000, Millipore, mab1527, Billerica, MA, USA).

    Techniques: Expressing, Immunofluorescence, Immunocytochemistry, Double Staining, Isolation

    Effects of GDF-15 on the kinetic properties of Nav1.8 channels. A. GDF-15 (1.2 nmol/L) does not shift the voltage-dependent activation curve of Nav1.8 channels. B. GDF-15 left-shifts the steady-state inactivation curve of Nav1.8 channels in a hyperpolarizing direction. C–F. GDF-15 (1.2 nmol/L) exaggerates the frequency-dependent reduction of Nav1.8 currents with increasing stimulation frequency from 1 to 10 Hz (* P

    Journal: Neuroscience Bulletin

    Article Title: Growth Differentiation Factor-15 Produces Analgesia by Inhibiting Tetrodotoxin-Resistant Nav1.8 Sodium Channel Activity in Rat Primary Sensory Neurons

    doi: 10.1007/s12264-021-00709-5

    Figure Lengend Snippet: Effects of GDF-15 on the kinetic properties of Nav1.8 channels. A. GDF-15 (1.2 nmol/L) does not shift the voltage-dependent activation curve of Nav1.8 channels. B. GDF-15 left-shifts the steady-state inactivation curve of Nav1.8 channels in a hyperpolarizing direction. C–F. GDF-15 (1.2 nmol/L) exaggerates the frequency-dependent reduction of Nav1.8 currents with increasing stimulation frequency from 1 to 10 Hz (* P

    Article Snippet: Sections were incubated in blocking solution (10% normal donkey serum in 0.01 mol/L PBS with 0.3% Triton X-100) for 2 h at RT, then overnight at 4°C with the following primary antibodies: goat anti-ALK2 (1:100, R & D Systems, AF637, Minneapolis, MN, USA), rabbit anti-Nav1.8 (1:200, ASC-016, Alomone, Jerusalem, Israel), rabbit anti-substance P (1:4000, Peninsula Labs, RIN7451, San Carlos, CA, USA), rabbit anti-CGRP, 1:20000, Peninsula Labs, IHC6006), mouse anti-peripherin (1:1000, Millipore, mab1527, Billerica, MA, USA).

    Techniques: Activation Assay