Journal: Biomedicines

Article Title: Vascular Effects of Low-Dose ACE2 Inhibitor MLN-4760—Benefit or Detriment in Essential Hypertension?

doi: 10.3390/biomedicines10010038

Figure Lengend Snippet: The equilibrium angiotensin peptides levels in plasma.

Article Snippet: Membranes were blocked with 5% nonfat milk for 1 h at room temperature in Tris-buffer solution (pH 7.6) containing 0.1% Tween-20 and probed against the following primary antibodies: rabbit polyclonal anti-endothelial NOS, anti-neuronal NOS and anti-β-actin (Abcam, Cambridge, UK); rabbit polyclonal anti-inducible NOS (Bio-Rad, Inc., Hercules, CA, USA) rabbit polyclonal anti-angiotensin(1–7) Mas receptor (Alomone Labs, Jerusalem, Israel); rabbit polyclonal CBS and mouse monoclonal anti-CSE antibodies (Proteintech, Manchester, UK); and rabbit monoclonal anti-ACE2 (Invitrogen, Waltham, MA, USA) overnight at 4 °C.

Techniques:

Journal: Biomedicines

Article Title: Vascular Effects of Low-Dose ACE2 Inhibitor MLN-4760—Benefit or Detriment in Essential Hypertension?

doi: 10.3390/biomedicines10010038

Figure Lengend Snippet: The correlation between the plasma level of the total alternative renin angiotensin system activity (Alt-S) and the weight of the visceral fat ( a ) and body weight ( b ). The correlation between the plasma level of angiotensin 1–7 (Ang 1–7) and the weight of the visceral fat ( c ) and body weight ( d ) in SHRs and SHRs treated with MLN-4760 (SHR + MLN).

Article Snippet: Membranes were blocked with 5% nonfat milk for 1 h at room temperature in Tris-buffer solution (pH 7.6) containing 0.1% Tween-20 and probed against the following primary antibodies: rabbit polyclonal anti-endothelial NOS, anti-neuronal NOS and anti-β-actin (Abcam, Cambridge, UK); rabbit polyclonal anti-inducible NOS (Bio-Rad, Inc., Hercules, CA, USA) rabbit polyclonal anti-angiotensin(1–7) Mas receptor (Alomone Labs, Jerusalem, Israel); rabbit polyclonal CBS and mouse monoclonal anti-CSE antibodies (Proteintech, Manchester, UK); and rabbit monoclonal anti-ACE2 (Invitrogen, Waltham, MA, USA) overnight at 4 °C.

Techniques: Activity Assay

Journal: Peptides

Article Title: Impact of angiotensin II type 1 and G-protein-coupled Mas receptor expression on the pulmonary performance of patients with idiopathic pulmonary fibrosis

doi: 10.1016/j.peptides.2020.170384

Figure Lengend Snippet: Protein expression of AT1 and Mas receptors in lung tissue (n = 12). A: Expression of AT1 and Mas receptors in idiopathic pulmonary fibrosis lung tissue and control evaluated by western blot. B: Protein quantification of AT1 receptor in idiopathic pulmonary fibrosis and control lung tissue. C: Protein quantification of Mas receptor in idiopathic pulmonary fibrosis and control lung tissue.

Article Snippet: Afterwards the membrane was stained with a 1:500 concentration of rabbit polyclonal anti-human anti-angiotensin II type-1 receptor antibody/AGTR1 (AAR-011, Alomone®, Israel) and a 1:250 concentration of rabbit polyclonal anti-human anti-angiotensin-(1–7) Mas receptor antibody (AAR-013, Alomone®, Israel) or mouse anti-human β-actin monoclonal antibody (A2228, Sigma Aldrich®, Germany), followed by secondary staining with a 1:1000 concentration of rabbit anti-mouse IgG (H + L)-HRP antibody (ThermoFisher Scientific®, MA, USA).

Techniques: Expressing, Western Blot

Journal: Peptides

Article Title: Impact of angiotensin II type 1 and G-protein-coupled Mas receptor expression on the pulmonary performance of patients with idiopathic pulmonary fibrosis

doi: 10.1016/j.peptides.2020.170384

Figure Lengend Snippet: Scatter plots of correlation analysis between spirometry values and AT1 receptor quantification (n = 12). FEV 1 =forced expiratory volume in the first second; FVC = forced vital capacity.

Article Snippet: Afterwards the membrane was stained with a 1:500 concentration of rabbit polyclonal anti-human anti-angiotensin II type-1 receptor antibody/AGTR1 (AAR-011, Alomone®, Israel) and a 1:250 concentration of rabbit polyclonal anti-human anti-angiotensin-(1–7) Mas receptor antibody (AAR-013, Alomone®, Israel) or mouse anti-human β-actin monoclonal antibody (A2228, Sigma Aldrich®, Germany), followed by secondary staining with a 1:1000 concentration of rabbit anti-mouse IgG (H + L)-HRP antibody (ThermoFisher Scientific®, MA, USA).

Techniques: