rabbit polyclonal anti h4 lys5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti h4 lys5
    Rabbit Polyclonal Anti H4 Lys5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti h4 lys5/product/Cell Signaling Technology Inc
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    94/100 stars

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    rabbit polyclonal histone h2b antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal histone h2b antibody
    A, Changes in PDUI score (-0.1 > PDUI > 0.1; P<0.05) denoting 3′-UTR-shortened (red) and lengthened (blue) genes. B, Genes showing lengthening (right) or shortening (left) events (-0.1> PDUI > 0.1; P<0.05) and are differentially expressed (FDR<0.05; fold change >1.5) as color coded. Up=upregulated gene expression, Down=downregulated gene expression. C, Venn diagram showing overlapping genes with significant APA alterations between JTE-607- treated and CPSF3 knockdown cells. D, Heatmap of differentially expressed genes in Panc1 cells treated with JTE-607. Expression is plotted as transformed expression value. Blue triangles denote replication-dependent histone genes. E, Gene set enrichment analysis (GSEA) of RNA-seq data from (D) . F, mRNA expression of <t>H2B</t> and H3 in MiaPaCa2 cells treated with JTE-607. * , P < 0.05, ** , P < 0.01, *** , P < 0.001, Ordinary one-way ANOVA with Dunnett’s multiple comparisons test.
    Rabbit Polyclonal Histone H2b Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "CPSF3 inhibition blocks pancreatic cancer cell proliferation through disruption of core histone processing"

    Article Title: CPSF3 inhibition blocks pancreatic cancer cell proliferation through disruption of core histone processing

    Journal: bioRxiv

    doi: 10.1101/2022.05.09.491230

    A, Changes in PDUI score (-0.1 > PDUI > 0.1; P<0.05) denoting 3′-UTR-shortened (red) and lengthened (blue) genes. B, Genes showing lengthening (right) or shortening (left) events (-0.1> PDUI > 0.1; P<0.05) and are differentially expressed (FDR<0.05; fold change >1.5) as color coded. Up=upregulated gene expression, Down=downregulated gene expression. C, Venn diagram showing overlapping genes with significant APA alterations between JTE-607- treated and CPSF3 knockdown cells. D, Heatmap of differentially expressed genes in Panc1 cells treated with JTE-607. Expression is plotted as transformed expression value. Blue triangles denote replication-dependent histone genes. E, Gene set enrichment analysis (GSEA) of RNA-seq data from (D) . F, mRNA expression of H2B and H3 in MiaPaCa2 cells treated with JTE-607. * , P < 0.05, ** , P < 0.01, *** , P < 0.001, Ordinary one-way ANOVA with Dunnett’s multiple comparisons test.
    Figure Legend Snippet: A, Changes in PDUI score (-0.1 > PDUI > 0.1; P<0.05) denoting 3′-UTR-shortened (red) and lengthened (blue) genes. B, Genes showing lengthening (right) or shortening (left) events (-0.1> PDUI > 0.1; P<0.05) and are differentially expressed (FDR<0.05; fold change >1.5) as color coded. Up=upregulated gene expression, Down=downregulated gene expression. C, Venn diagram showing overlapping genes with significant APA alterations between JTE-607- treated and CPSF3 knockdown cells. D, Heatmap of differentially expressed genes in Panc1 cells treated with JTE-607. Expression is plotted as transformed expression value. Blue triangles denote replication-dependent histone genes. E, Gene set enrichment analysis (GSEA) of RNA-seq data from (D) . F, mRNA expression of H2B and H3 in MiaPaCa2 cells treated with JTE-607. * , P < 0.05, ** , P < 0.01, *** , P < 0.001, Ordinary one-way ANOVA with Dunnett’s multiple comparisons test.

    Techniques Used: Expressing, Transformation Assay, RNA Sequencing Assay

    A, IGV-generated density plots of replication-dependent histones highlighting the differences of 3′- UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. B, Western blot of H2B and H3 protein levels in Panc1 cells treated with 0-10μM JTE-607 for 24 and 48hrs. C, IGV-generated density plots of replication-independent histones highlighting the differences of 3′-UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. D, DSeq2 normalized counts of H2AFZ and H3F3A histone variants in Panc1 cells treated with JTE-607. ** , P < 0.001. E-G, Volcano plots of Spearman’s correlation of CPSF3 and: E, all histone genes; F, replication-dependent histone genes; G, replication-independent histone genes. Each dot represents a histone gene. Blue and red dots denote positive and negative correlation, respectively. (Spearman = -0.15>R>0.15, P<0.05).
    Figure Legend Snippet: A, IGV-generated density plots of replication-dependent histones highlighting the differences of 3′- UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. B, Western blot of H2B and H3 protein levels in Panc1 cells treated with 0-10μM JTE-607 for 24 and 48hrs. C, IGV-generated density plots of replication-independent histones highlighting the differences of 3′-UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. D, DSeq2 normalized counts of H2AFZ and H3F3A histone variants in Panc1 cells treated with JTE-607. ** , P < 0.001. E-G, Volcano plots of Spearman’s correlation of CPSF3 and: E, all histone genes; F, replication-dependent histone genes; G, replication-independent histone genes. Each dot represents a histone gene. Blue and red dots denote positive and negative correlation, respectively. (Spearman = -0.15>R>0.15, P<0.05).

    Techniques Used: Generated, Western Blot

    rabbit polyclonal anti histone h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti histone h2b
    KEY RESOURCES TABLE
    Rabbit Polyclonal Anti Histone H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency"

    Article Title: Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2021.06.011

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Electron Microscopy, Plasmid Preparation, Imaging, CCK-8 Assay, CRISPR, shRNA, Negative Control, Construct, Software, Transfection, DNA Extraction

    rabbit polyclonal anti acetyl histone h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti acetyl histone h2b
    Rabbit Polyclonal Anti Acetyl Histone H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polyclonal rabbit antihuman acetylated histone 2b lysine 5 antibodies  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc polyclonal rabbit antihuman acetylated histone 2b lysine 5 antibodies
    Polyclonal Rabbit Antihuman Acetylated Histone 2b Lysine 5 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit antihuman acetylated histone 2b lysine 5 antibodies/product/Cell Signaling Technology Inc
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    rabbit polyclonal anti h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti h2b
    (A) Western blotting of ub-H2A, <t>ub-H2B,</t> and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
    Rabbit Polyclonal Anti H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis"

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    Journal: Cell

    doi: 10.1016/j.cell.2017.04.034

    (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
    Figure Legend Snippet: (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).

    Techniques Used: Western Blot

    (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.
    Figure Legend Snippet: (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.

    Techniques Used: Immunostaining, Staining, Transduction, Construct, Translocation Assay

    Key Resources Table
    Figure Legend Snippet: Key Resources Table

    Techniques Used: Recombinant, In Situ, Sequencing, Software

    rabbit polyclonal anti ub h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti ub h2b
    (A) Western blotting of ub-H2A, <t>ub-H2B,</t> and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
    Rabbit Polyclonal Anti Ub H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti ub h2b/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti ub h2b - by Bioz Stars, 2024-06
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    Images

    1) Product Images from "Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis"

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    Journal: Cell

    doi: 10.1016/j.cell.2017.04.034

    (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
    Figure Legend Snippet: (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).

    Techniques Used: Western Blot

    (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.
    Figure Legend Snippet: (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.

    Techniques Used: Immunostaining, Staining, Transduction, Construct, Translocation Assay

    Key Resources Table
    Figure Legend Snippet: Key Resources Table

    Techniques Used: Recombinant, In Situ, Sequencing, Software

    rabbit polyclonal anti h2b k120ub  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti h2b k120ub
    Rabbit Polyclonal Anti H2b K120ub, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti h2b k120ub/product/Cell Signaling Technology Inc
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    rabbit polyclonal anti h2b k120ub - by Bioz Stars, 2024-06
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    rabbit polyclonal anti h2b k120ub  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti h2b k120ub
    Rabbit Polyclonal Anti H2b K120ub, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti h2b k120ub/product/Cell Signaling Technology Inc
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    rabbit polyclonal anti acetyl h2bk5 igg  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti acetyl h2bk5 igg
    Rabbit Polyclonal Anti Acetyl H2bk5 Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti acetyl h2bk5 igg/product/Cell Signaling Technology Inc
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    rabbit polyclonal anti acetyl h2bk5 igg - by Bioz Stars, 2024-06
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    Cell Signaling Technology Inc rabbit polyclonal anti h4 lys5
    Rabbit Polyclonal Anti H4 Lys5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A, Changes in PDUI score (-0.1 > PDUI > 0.1; P<0.05) denoting 3′-UTR-shortened (red) and lengthened (blue) genes. B, Genes showing lengthening (right) or shortening (left) events (-0.1> PDUI > 0.1; P<0.05) and are differentially expressed (FDR<0.05; fold change >1.5) as color coded. Up=upregulated gene expression, Down=downregulated gene expression. C, Venn diagram showing overlapping genes with significant APA alterations between JTE-607- treated and CPSF3 knockdown cells. D, Heatmap of differentially expressed genes in Panc1 cells treated with JTE-607. Expression is plotted as transformed expression value. Blue triangles denote replication-dependent histone genes. E, Gene set enrichment analysis (GSEA) of RNA-seq data from (D) . F, mRNA expression of <t>H2B</t> and H3 in MiaPaCa2 cells treated with JTE-607. * , P < 0.05, ** , P < 0.01, *** , P < 0.001, Ordinary one-way ANOVA with Dunnett’s multiple comparisons test.
    Rabbit Polyclonal Histone H2b Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    KEY RESOURCES TABLE
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    (A) Western blotting of ub-H2A, <t>ub-H2B,</t> and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
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    (A) Western blotting of ub-H2A, <t>ub-H2B,</t> and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
    Rabbit Polyclonal Anti Ub H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Western blotting of ub-H2A, <t>ub-H2B,</t> and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
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    (A) Western blotting of ub-H2A, <t>ub-H2B,</t> and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).
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    A, Changes in PDUI score (-0.1 > PDUI > 0.1; P<0.05) denoting 3′-UTR-shortened (red) and lengthened (blue) genes. B, Genes showing lengthening (right) or shortening (left) events (-0.1> PDUI > 0.1; P<0.05) and are differentially expressed (FDR<0.05; fold change >1.5) as color coded. Up=upregulated gene expression, Down=downregulated gene expression. C, Venn diagram showing overlapping genes with significant APA alterations between JTE-607- treated and CPSF3 knockdown cells. D, Heatmap of differentially expressed genes in Panc1 cells treated with JTE-607. Expression is plotted as transformed expression value. Blue triangles denote replication-dependent histone genes. E, Gene set enrichment analysis (GSEA) of RNA-seq data from (D) . F, mRNA expression of H2B and H3 in MiaPaCa2 cells treated with JTE-607. * , P < 0.05, ** , P < 0.01, *** , P < 0.001, Ordinary one-way ANOVA with Dunnett’s multiple comparisons test.

    Journal: bioRxiv

    Article Title: CPSF3 inhibition blocks pancreatic cancer cell proliferation through disruption of core histone processing

    doi: 10.1101/2022.05.09.491230

    Figure Lengend Snippet: A, Changes in PDUI score (-0.1 > PDUI > 0.1; P<0.05) denoting 3′-UTR-shortened (red) and lengthened (blue) genes. B, Genes showing lengthening (right) or shortening (left) events (-0.1> PDUI > 0.1; P<0.05) and are differentially expressed (FDR<0.05; fold change >1.5) as color coded. Up=upregulated gene expression, Down=downregulated gene expression. C, Venn diagram showing overlapping genes with significant APA alterations between JTE-607- treated and CPSF3 knockdown cells. D, Heatmap of differentially expressed genes in Panc1 cells treated with JTE-607. Expression is plotted as transformed expression value. Blue triangles denote replication-dependent histone genes. E, Gene set enrichment analysis (GSEA) of RNA-seq data from (D) . F, mRNA expression of H2B and H3 in MiaPaCa2 cells treated with JTE-607. * , P < 0.05, ** , P < 0.01, *** , P < 0.001, Ordinary one-way ANOVA with Dunnett’s multiple comparisons test.

    Article Snippet: Primary antibodies were diluted in 3% BSA in TBST and incubated overnight at 4°C (mouse monoclonal CPSF3 antibody, Abcepta, AT1610a; rabbit polyclonal Histone H3 antibody, Cell Signaling Technology, 9715S; rabbit polyclonal Histone H2B antibody, Cell Signaling Technology, 8135S; mouse monoclonal GAPDH antibody, Proteintech, 60004-1-Ig; rabbit polyclonal FHL1 antibody, Proteintech, 10991-1-AP).

    Techniques: Expressing, Transformation Assay, RNA Sequencing Assay

    A, IGV-generated density plots of replication-dependent histones highlighting the differences of 3′- UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. B, Western blot of H2B and H3 protein levels in Panc1 cells treated with 0-10μM JTE-607 for 24 and 48hrs. C, IGV-generated density plots of replication-independent histones highlighting the differences of 3′-UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. D, DSeq2 normalized counts of H2AFZ and H3F3A histone variants in Panc1 cells treated with JTE-607. ** , P < 0.001. E-G, Volcano plots of Spearman’s correlation of CPSF3 and: E, all histone genes; F, replication-dependent histone genes; G, replication-independent histone genes. Each dot represents a histone gene. Blue and red dots denote positive and negative correlation, respectively. (Spearman = -0.15>R>0.15, P<0.05).

    Journal: bioRxiv

    Article Title: CPSF3 inhibition blocks pancreatic cancer cell proliferation through disruption of core histone processing

    doi: 10.1101/2022.05.09.491230

    Figure Lengend Snippet: A, IGV-generated density plots of replication-dependent histones highlighting the differences of 3′- UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. B, Western blot of H2B and H3 protein levels in Panc1 cells treated with 0-10μM JTE-607 for 24 and 48hrs. C, IGV-generated density plots of replication-independent histones highlighting the differences of 3′-UTR coverage between DMSO (red) and JTE-607 (blue) treated cells. D, DSeq2 normalized counts of H2AFZ and H3F3A histone variants in Panc1 cells treated with JTE-607. ** , P < 0.001. E-G, Volcano plots of Spearman’s correlation of CPSF3 and: E, all histone genes; F, replication-dependent histone genes; G, replication-independent histone genes. Each dot represents a histone gene. Blue and red dots denote positive and negative correlation, respectively. (Spearman = -0.15>R>0.15, P<0.05).

    Article Snippet: Primary antibodies were diluted in 3% BSA in TBST and incubated overnight at 4°C (mouse monoclonal CPSF3 antibody, Abcepta, AT1610a; rabbit polyclonal Histone H3 antibody, Cell Signaling Technology, 9715S; rabbit polyclonal Histone H2B antibody, Cell Signaling Technology, 8135S; mouse monoclonal GAPDH antibody, Proteintech, 60004-1-Ig; rabbit polyclonal FHL1 antibody, Proteintech, 10991-1-AP).

    Techniques: Generated, Western Blot

    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency

    doi: 10.1016/j.molcel.2021.06.011

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit polyclonal anti-Histone H2B (V119) , Cell Signaling Technology , Cat# 8135; RRID: AB_10891053.

    Techniques: Recombinant, Electron Microscopy, Plasmid Preparation, Imaging, CCK-8 Assay, CRISPR, shRNA, Negative Control, Construct, Software, Transfection, DNA Extraction

    (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).

    Journal: Cell

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    doi: 10.1016/j.cell.2017.04.034

    Figure Lengend Snippet: (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).

    Article Snippet: Rabbit polyclonal anti-H2B , Cell Signaling Technology , Cat#12364.

    Techniques: Western Blot

    (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.

    Journal: Cell

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    doi: 10.1016/j.cell.2017.04.034

    Figure Lengend Snippet: (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.

    Article Snippet: Rabbit polyclonal anti-H2B , Cell Signaling Technology , Cat#12364.

    Techniques: Immunostaining, Staining, Transduction, Construct, Translocation Assay

    Key Resources Table

    Journal: Cell

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    doi: 10.1016/j.cell.2017.04.034

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: Rabbit polyclonal anti-H2B , Cell Signaling Technology , Cat#12364.

    Techniques: Recombinant, In Situ, Sequencing, Software

    (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).

    Journal: Cell

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    doi: 10.1016/j.cell.2017.04.034

    Figure Lengend Snippet: (A) Western blotting of ub-H2A, ub-H2B, and H4K16ac in late spermatids from dpp 12-week mice with H3K27me3 as a loading control. Quantification of blotting intensity for indicated proteins is shown in parentheses (the one in Miwi+/DB control is set as 100% after normalization with H3K27me3 blotting).

    Article Snippet: Rabbit polyclonal anti-ub-H2B , Cell Signaling Technology , Cat#5546; RRID:AB_10693452.

    Techniques: Western Blot

    (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.

    Journal: Cell

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    doi: 10.1016/j.cell.2017.04.034

    Figure Lengend Snippet: (A–C) Immunostaining of endogenous RNF8 (A), (red) or ub-H2B (B), (red) in late spermatids (LS) and H2B in sperm (C), (red) from Miwi+/DB-Cre males transduced by RNF8-N:Cyto IV-EGFP (left) or RNF8-Nmut:Cyto IV-EGFP (right), with nuclei counterstained by DAPI (blue). Left lanes in each staining panel show the representative staining images, and GFP (green) and arrows indicate cells that were successfully transduced with the respective constructs; right lanes show an enlarged view of the representative cells. Right panels: quantification of late spermatids showing visible RNF8 nucleus translocation (A) or ub-H2B staining (B), and sperm without H2B staining (C), top) or with normal morphology (C), bottom) among GFP+ or GFP− cells (n = 100 per group; the average values ± SD of three separate experiments are plotted). Scale bar, 10 μm.

    Article Snippet: Rabbit polyclonal anti-ub-H2B , Cell Signaling Technology , Cat#5546; RRID:AB_10693452.

    Techniques: Immunostaining, Staining, Transduction, Construct, Translocation Assay

    Key Resources Table

    Journal: Cell

    Article Title: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis

    doi: 10.1016/j.cell.2017.04.034

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: Rabbit polyclonal anti-ub-H2B , Cell Signaling Technology , Cat#5546; RRID:AB_10693452.

    Techniques: Recombinant, In Situ, Sequencing, Software