Journal: eLife

Article Title: The nutrient-sensing GCN2 signaling pathway is essential for circadian clock function by regulating histone acetylation under amino acid starvation

doi: 10.7554/eLife.85241

Figure Lengend Snippet: ( A ) Race tube assay showing that the conidiation rhythm in gcn-5 KO strain was lost compared with WT strain. ( B ) Luciferase assay showing that the luciferase activity rhythm was impaired in the gcn-5 KO strain after 1 day transition from light to dark. A FRQ-LUC translational fusion construct was expressed in WT and gcn-5 KO strains, and the luciferase signal was recorded in DD for more than 7 days. Normalized data with the baseline luciferase signals subtracted are shown. ( C ) Western blot assay showing that rhythmic expression of FRQ protein was dampened in the gcn-5 KO strain ( n = 3; WT: p = 5.00E−08, gcn-5 KO : p = 0.0016, RAIN; WT vs gcn-5 KO : mesor p = 0.1421, amplitude p = 0.0774, phase p = 0.4319, CircaCompare). RT-qPCR analysis showing that rhythmic expression of frq mRNA was dampened in the gcn-5 KO strain without 3-aminotriazole (3-AT; n = 3; WT: p = 8.37E−06, gcn-5 KO : p > 0.05) ( D ) or with 3-AT ( n = 3; WT: p = 5.39E−12, gcn-5 KO : p > 0.05) ( E ). ( F ) Chromatin immunoprecipitation (ChIP) assay showing decreased histone H3ac levels at the promoter of frq gene in the gcn-5 KO strain at the indicated time points in DD ( n = 3; WT: p = 8.10E−05, gcn-5 KO : p > 0.05). Relative H3ac levels were normalized with H2B levels. ( G ) ChIP assay showing decreased WC-2 levels at the promoter of frq gene in the gcn-5 KO strain at the indicated time points in DD ( n = 3; WT: p = 0.0003, gcn-5 KO : p = 0.0459, RAIN; WT vs gcn-5 KO : mesor p = 0.2939, amplitude p = 0.0010, phase p = 0.6933, CircaCompare). Error bars indicate standard deviations ( n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; Student’s t test was used. Figure 5—source data 1. LumiCycle analysis dataset in . Figure 5—source data 2. Scan of western blot probed for FRQ protein and quantification dataset in . Figure 5—source data 3. RT-qPCR analysis dataset in . Figure 5—source data 4. RT-qPCR analysis dataset in . Figure 5—source data 5. Chromatin immunoprecipitation (ChIP) analysis dataset in . Figure 5—source data 6. Chromatin immunoprecipitation (ChIP) analysis dataset in .

Article Snippet: Antibody , Rabbit polyclonal anti-Histone H2B , Abcam , Cat# ab1790 , 1:3000.

Techniques: Luciferase, Activity Assay, Construct, Western Blot, Expressing, Quantitative RT-PCR, Chromatin Immunoprecipitation

Journal: eLife

Article Title: The nutrient-sensing GCN2 signaling pathway is essential for circadian clock function by regulating histone acetylation under amino acid starvation

doi: 10.7554/eLife.85241

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-Histone H2B , Abcam , Cat# ab1790 , 1:3000.

Techniques: Sequencing, Northern Blot, Software

Journal: PLoS ONE

Article Title: Loss of the Histone Pre-mRNA Processing Factor Stem-Loop Binding Protein in Drosophila Causes Genomic Instability and Impaired Cellular Proliferation

doi: 10.1371/journal.pone.0008168

Figure Lengend Snippet: Protein lysates from wt, Slbp 15 , Slbp 10 , and H2aV 810 mutants were obtained from whole 3 rd instar larvae and probed with antibodies to A) H2b and H3, and B) H3K4-me2 and H3K9-me2. β-tubulin was used as a loading control for both panels.

Article Snippet: Polyclonal rabbit C-terminal H3 (1∶3000) (Abcam #1791), polyclonal rabbit H2b (Abcam #1790) (1∶3000), monoclonal mouse H3K9-me2 (Abcam #1220) (1∶750), and polyclonal rabbit H3K4-me2 (Abcam #7766) (1∶3000), and monoclonal mouse Β-tubulin (1∶1000) antibodies were obtained commercially.

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Combination of Coenzyme Q 10 Intake and Moderate Physical Activity Counteracts Mitochondrial Dysfunctions in a SAMP8 Mouse Model

doi: 10.1155/2018/8936251

Figure Lengend Snippet: Summary of used antibodies.

Article Snippet: H2B , Rabbit polyclonal anti-H2B , 1 : 1000 , Abcam.

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Combination of Coenzyme Q 10 Intake and Moderate Physical Activity Counteracts Mitochondrial Dysfunctions in a SAMP8 Mouse Model

doi: 10.1155/2018/8936251

Figure Lengend Snippet: Western blot analysis (a) and relative protein quantification (b) of PGC-1 α , TFAM, and SIRT5 expressed in tibialis anterior (TA) muscle, in sedentary (SED), physical exercise (PHY), ubiquinol (QH 2 ), and ubiquinol associated with physical exercise (QH 2 + PHY) mouse groups ( n = 5). PGC-1 α protein levels were normalized to H2B levels. TFAM and SIRT5 were normalized to VDAC1 levels. AU: arbitrary units. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001; (a) = vs. SED, (b) = vs. PHY, and (c) = vs. QH 2 .

Article Snippet: H2B , Rabbit polyclonal anti-H2B , 1 : 1000 , Abcam.

Techniques: Western Blot