rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh  (Millipore)


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    Structured Review

    Millipore rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh
    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to <t>glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH),</t> which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.
    Rabbit Polyclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 84 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-03
    84/100 stars

    Images

    1) Product Images from "Impaired structural and functional regeneration of skeletal muscles from β2-adrenoceptor knockout mice"

    Article Title: Impaired structural and functional regeneration of skeletal muscles from β2-adrenoceptor knockout mice

    Journal: Acta Physiologica (Oxford, England)

    doi: 10.1111/apha.12329

    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.
    Figure Legend Snippet: Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.

    Techniques Used: Expressing, Western Blot, Mouse Assay, SDS Page, Molecular Weight

    Related Articles

    Electron Microscopy:

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma. .. The immunogold secondary antibodies were purchased from Electron Microscopy Sciences and included goat anti-mouse 15-nm gold-conjugated (1:20) and goat anti-rabbit 6-nm gold-conjugated (1:25) antibodies for purified VLP labeling.

    Fluorescence:

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: Fluorescence-activated cell sorter (FACS) analysis was performed with the anti-N2 antibody mentioned above. .. The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma.

    Electroporation:

    Article Title: Desmin Mutation in the C-Terminal Domain Impairs Traction Force Generation in Myoblasts
    Article Snippet: 24 h after electroporation culture dishes containing cells were washed three times in PBS and proteins were extracted with RIPA buffer (50 mM Tris, 150 mM NaCl, 1% NP40, 5 mM EDTA, 1 mM NA3 VO4 , 10 mM NaF, PMSF 1 mM, and antiprotease mix from Sigma-Aldrich). .. Primary antibodies were then added: Rabbit polyclonal antidesmin (Biogenesis England, Biotechnologies) at 1:250, mouse monoclonal anti- α -actin (clone 4, Merck Millipore, Darmstadt, Germany) at 1:2000, rabbit monoclonal antivimentin at 1:5000 (AbCam), rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) at 1:15000, or rabbit polyclonal anti-GFP (Life Technologies) at 1:3000.

    Labeling:

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: 2G9 was used for immunogold labeling of the VLPs, while 4B2 and the rabbit polyclonal antibody were used for Western blot analysis. .. The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma.

    Purification:

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma. .. The immunogold secondary antibodies were purchased from Electron Microscopy Sciences and included goat anti-mouse 15-nm gold-conjugated (1:20) and goat anti-rabbit 6-nm gold-conjugated (1:25) antibodies for purified VLP labeling.

    Electrophoresis:

    Article Title: Desmin Mutation in the C-Terminal Domain Impairs Traction Force Generation in Myoblasts
    Article Snippet: Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis on a 10% acrylamid-bisacrylamide gel and transferred to nitrocellulose membranes (Macherey Nagel, Duren, Germany). .. Primary antibodies were then added: Rabbit polyclonal antidesmin (Biogenesis England, Biotechnologies) at 1:250, mouse monoclonal anti- α -actin (clone 4, Merck Millipore, Darmstadt, Germany) at 1:2000, rabbit monoclonal antivimentin at 1:5000 (AbCam), rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) at 1:15000, or rabbit polyclonal anti-GFP (Life Technologies) at 1:3000.

    Generated:

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: The E10 anti-M1 and -M2 antibody was generated in our laboratory and was described previously ( ). .. The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Desmin Mutation in the C-Terminal Domain Impairs Traction Force Generation in Myoblasts
    Article Snippet: Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis on a 10% acrylamid-bisacrylamide gel and transferred to nitrocellulose membranes (Macherey Nagel, Duren, Germany). .. Primary antibodies were then added: Rabbit polyclonal antidesmin (Biogenesis England, Biotechnologies) at 1:250, mouse monoclonal anti- α -actin (clone 4, Merck Millipore, Darmstadt, Germany) at 1:2000, rabbit monoclonal antivimentin at 1:5000 (AbCam), rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) at 1:15000, or rabbit polyclonal anti-GFP (Life Technologies) at 1:3000.

    Western Blot:

    Article Title: Impaired structural and functional regeneration of skeletal muscles from β2-adrenoceptor knockout mice
    Article Snippet: .. Antibodies for Western blotting The primary antibodies used for Western blotting were (1) rabbit polyclonal anti-Smad4 (1 : 1000; cat# 9515; Cell Signaling Technology), (2) rabbit polyclonal anti-akirin1 (1 : 1000; cat# ab77075; Abcam) and (3) rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1 : 2000; cat# ABS16; Millipore, Temecula, CA, USA). .. The corresponding secondary antibody used for all Western blots was peroxidase-conjugated AffiniPure goat anti-rabbit IgG (1 : 10 000; cat# 111-035-003; Jackson Immuno Research Laboratories).

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: 2G9 was used for immunogold labeling of the VLPs, while 4B2 and the rabbit polyclonal antibody were used for Western blot analysis. .. The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma.

    Article Title: Desmin Mutation in the C-Terminal Domain Impairs Traction Force Generation in Myoblasts
    Article Snippet: Paragraph title: Western blot analysis ... Primary antibodies were then added: Rabbit polyclonal antidesmin (Biogenesis England, Biotechnologies) at 1:250, mouse monoclonal anti- α -actin (clone 4, Merck Millipore, Darmstadt, Germany) at 1:2000, rabbit monoclonal antivimentin at 1:5000 (AbCam), rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) at 1:15000, or rabbit polyclonal anti-GFP (Life Technologies) at 1:3000.

    FACS:

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: Fluorescence-activated cell sorter (FACS) analysis was performed with the anti-N2 antibody mentioned above. .. The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma.

    Immunofluorescence:

    Article Title: Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin ▿
    Article Snippet: The rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control antibody was purchased from Sigma. .. The secondary antibodies used included a donkey anti-goat fluorescein isothiocyanate (FITC)-conjugated antibody for FACS analysis and indirect immunofluorescence.

    SDS Page:

    Article Title: Desmin Mutation in the C-Terminal Domain Impairs Traction Force Generation in Myoblasts
    Article Snippet: Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis on a 10% acrylamid-bisacrylamide gel and transferred to nitrocellulose membranes (Macherey Nagel, Duren, Germany). .. Primary antibodies were then added: Rabbit polyclonal antidesmin (Biogenesis England, Biotechnologies) at 1:250, mouse monoclonal anti- α -actin (clone 4, Merck Millipore, Darmstadt, Germany) at 1:2000, rabbit monoclonal antivimentin at 1:5000 (AbCam), rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) at 1:15000, or rabbit polyclonal anti-GFP (Life Technologies) at 1:3000.

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  • 90
    Millipore rabbit anti bax
    Effects of coffee oil-algae oil nanoemulsions on protein expressions of <t>cyclin</t> B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and <t>Bax,</t> Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p
    Rabbit Anti Bax, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax/product/Millipore
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rabbit anti bax - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

    84
    Millipore rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh
    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to <t>glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH),</t> which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.
    Rabbit Polyclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 84 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-03
    84/100 stars
      Buy from Supplier

    82
    Millipore rabbit anti bax antibody
    Relationship of BNIP3 expression to <t>Bax</t> activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control <t>COX</t> IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.
    Rabbit Anti Bax Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax antibody/product/Millipore
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti bax antibody - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    Image Search Results


    Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Journal: International Journal of Nanomedicine

    Article Title: Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

    doi: 10.2147/IJN.S144705

    Figure Lengend Snippet: Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Article Snippet: The primary antibodies include mouse monoclonal anti-α-tubulin antibody (Sigma–Aldrich Co.), mouse anti-cyclin A and rabbit anti-Bax (EMD Millipore), mouse anti-CDK2, mouse anti-cytochrome C, mouse anti-p21, mouse anti-cyclin B, and mouse anti-Bcl-2 (BD Biosciences), as well as anti-cdc2 (CDK1) and mouse anti-p53 (Novus Biologicals Co., Littleton, CO, USA).

    Techniques: Incubation, Standard Deviation

    L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Journal: BMC Medicine

    Article Title: Sodium vanadate combined with l-ascorbic acid delays disease progression, enhances motor performance, and ameliorates muscle atrophy and weakness in mice with spinal muscular atrophy

    doi: 10.1186/1741-7015-11-38

    Figure Lengend Snippet: L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Article Snippet: Primary antibodies used for western blotting included mouse anti-SMN (1:5,000; BD Biosciences, San Diego, CA, USA), mouse anti-β-actin (1:10,000; Sigma), rabbit anti-Bax (1:1,000; Millipore, Temecula, CA, USA), and rabbit anti-caspase 3 (1:1,000; Cell Signaling, Temecula, CA, USA).

    Techniques: Western Blot, Expressing

    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.

    Journal: Acta Physiologica (Oxford, England)

    Article Title: Impaired structural and functional regeneration of skeletal muscles from β2-adrenoceptor knockout mice

    doi: 10.1111/apha.12329

    Figure Lengend Snippet: Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.

    Article Snippet: Antibodies for Western blotting The primary antibodies used for Western blotting were (1) rabbit polyclonal anti-Smad4 (1 : 1000; cat# 9515; Cell Signaling Technology), (2) rabbit polyclonal anti-akirin1 (1 : 1000; cat# ab77075; Abcam) and (3) rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1 : 2000; cat# ABS16; Millipore, Temecula, CA, USA).

    Techniques: Expressing, Western Blot, Mouse Assay, SDS Page, Molecular Weight

    Relationship of BNIP3 expression to Bax activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control COX IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: Cyanide-Induced Apoptosis of Dopaminergic Cells Is Promoted by BNIP3 and Bax Modulation of Endoplasmic Reticulum-Mitochondrial Ca2+ Levels S⃞

    doi: 10.1124/jpet.109.159103

    Figure Lengend Snippet: Relationship of BNIP3 expression to Bax activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control COX IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.

    Article Snippet: The primary antibodies were: mouse anti-BNIP3 antibody, mouse anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO), mouse anti-cytochrome c oxidase (COX IV), rabbit anticalnexin antibodies (Abcam Inc., Cambridge, MA), rabbit anti-Bax antibody (Millipore, Billerica, MA), and mouse anti-Bax antibody (Santa Cruz Biotechnology, San Cruz, CA).

    Techniques: Expressing, Activation Assay, Transfection, Western Blot, Plasmid Preparation