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rabbit polyclonal anti eb2 phospho ser 222  (Covalab Inc)

 
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    Structured Review

    Covalab Inc rabbit polyclonal anti eb2 phospho ser 222
    Phosphorylation of the exogenous GFP-CDKL5 TEY motif and endogenous <t>EB2</t> are restored following ACE-tRNA Arg UGA delivery. ( a , c ) Representative western blots for CDKL5 pTyr 171 ( a ) and EB2 pSer 222 ( c ) in HEK293T co-transfected with GFP, WT GFP-CDKL5 or -R59X, -R134X, -R550X constructs with or without 1X or 4X ACE-tRNA Arg UGA . CDKL5 phosphorylation at Tyr 171 was revealed together with full-length CDKL5 (through an anti-GFP antibody). White arrowheads show the premature truncated GFP-CDKL5 R550X derivative that was not subjected to readthrough. Original images of blots in a are shown in the Supplementary Information file. ( b , d ) Histograms depict the percentages of full-length GFP-CDKL5 phosphorylation at Tyr 171 ( b ) and of EB2 phosphorylation at Ser 222 ( d ) with respect to untreated (UT) GFP-CDKL5 WT. For analysis of phosphorylated EB2, for each sample data were normalized to the total levels of corresponding protein. Data are expressed as mean ± SEM. * p < 0.05 by Kruskal-Wallis test followed by Dunn’s multiple comparison test.
    Rabbit Polyclonal Anti Eb2 Phospho Ser 222, supplied by Covalab Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti eb2 phospho ser 222/product/Covalab Inc
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti eb2 phospho ser 222 - by Bioz Stars, 2025-03
    86/100 stars

    Images

    1) Product Images from "Engineered tRNAs efficiently suppress CDKL5 premature termination codons"

    Article Title: Engineered tRNAs efficiently suppress CDKL5 premature termination codons

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-82766-0

    Phosphorylation of the exogenous GFP-CDKL5 TEY motif and endogenous EB2 are restored following ACE-tRNA Arg UGA delivery. ( a , c ) Representative western blots for CDKL5 pTyr 171 ( a ) and EB2 pSer 222 ( c ) in HEK293T co-transfected with GFP, WT GFP-CDKL5 or -R59X, -R134X, -R550X constructs with or without 1X or 4X ACE-tRNA Arg UGA . CDKL5 phosphorylation at Tyr 171 was revealed together with full-length CDKL5 (through an anti-GFP antibody). White arrowheads show the premature truncated GFP-CDKL5 R550X derivative that was not subjected to readthrough. Original images of blots in a are shown in the Supplementary Information file. ( b , d ) Histograms depict the percentages of full-length GFP-CDKL5 phosphorylation at Tyr 171 ( b ) and of EB2 phosphorylation at Ser 222 ( d ) with respect to untreated (UT) GFP-CDKL5 WT. For analysis of phosphorylated EB2, for each sample data were normalized to the total levels of corresponding protein. Data are expressed as mean ± SEM. * p < 0.05 by Kruskal-Wallis test followed by Dunn’s multiple comparison test.
    Figure Legend Snippet: Phosphorylation of the exogenous GFP-CDKL5 TEY motif and endogenous EB2 are restored following ACE-tRNA Arg UGA delivery. ( a , c ) Representative western blots for CDKL5 pTyr 171 ( a ) and EB2 pSer 222 ( c ) in HEK293T co-transfected with GFP, WT GFP-CDKL5 or -R59X, -R134X, -R550X constructs with or without 1X or 4X ACE-tRNA Arg UGA . CDKL5 phosphorylation at Tyr 171 was revealed together with full-length CDKL5 (through an anti-GFP antibody). White arrowheads show the premature truncated GFP-CDKL5 R550X derivative that was not subjected to readthrough. Original images of blots in a are shown in the Supplementary Information file. ( b , d ) Histograms depict the percentages of full-length GFP-CDKL5 phosphorylation at Tyr 171 ( b ) and of EB2 phosphorylation at Ser 222 ( d ) with respect to untreated (UT) GFP-CDKL5 WT. For analysis of phosphorylated EB2, for each sample data were normalized to the total levels of corresponding protein. Data are expressed as mean ± SEM. * p < 0.05 by Kruskal-Wallis test followed by Dunn’s multiple comparison test.

    Techniques Used: Western Blot, Transfection, Construct, Comparison



    Similar Products

    rabbit polyclonal anti eb2 phospho ser 222  (Covalab Inc)
    86
    Covalab Inc rabbit polyclonal anti eb2 phospho ser 222
    Phosphorylation of the exogenous GFP-CDKL5 TEY motif and endogenous <t>EB2</t> are restored following ACE-tRNA Arg UGA delivery. ( a , c ) Representative western blots for CDKL5 pTyr 171 ( a ) and EB2 pSer 222 ( c ) in HEK293T co-transfected with GFP, WT GFP-CDKL5 or -R59X, -R134X, -R550X constructs with or without 1X or 4X ACE-tRNA Arg UGA . CDKL5 phosphorylation at Tyr 171 was revealed together with full-length CDKL5 (through an anti-GFP antibody). White arrowheads show the premature truncated GFP-CDKL5 R550X derivative that was not subjected to readthrough. Original images of blots in a are shown in the Supplementary Information file. ( b , d ) Histograms depict the percentages of full-length GFP-CDKL5 phosphorylation at Tyr 171 ( b ) and of EB2 phosphorylation at Ser 222 ( d ) with respect to untreated (UT) GFP-CDKL5 WT. For analysis of phosphorylated EB2, for each sample data were normalized to the total levels of corresponding protein. Data are expressed as mean ± SEM. * p < 0.05 by Kruskal-Wallis test followed by Dunn’s multiple comparison test.
    Rabbit Polyclonal Anti Eb2 Phospho Ser 222, supplied by Covalab Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti eb2 phospho ser 222/product/Covalab Inc
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti eb2 phospho ser 222 - by Bioz Stars, 2025-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Phosphorylation of the exogenous GFP-CDKL5 TEY motif and endogenous EB2 are restored following ACE-tRNA Arg UGA delivery. ( a , c ) Representative western blots for CDKL5 pTyr 171 ( a ) and EB2 pSer 222 ( c ) in HEK293T co-transfected with GFP, WT GFP-CDKL5 or -R59X, -R134X, -R550X constructs with or without 1X or 4X ACE-tRNA Arg UGA . CDKL5 phosphorylation at Tyr 171 was revealed together with full-length CDKL5 (through an anti-GFP antibody). White arrowheads show the premature truncated GFP-CDKL5 R550X derivative that was not subjected to readthrough. Original images of blots in a are shown in the Supplementary Information file. ( b , d ) Histograms depict the percentages of full-length GFP-CDKL5 phosphorylation at Tyr 171 ( b ) and of EB2 phosphorylation at Ser 222 ( d ) with respect to untreated (UT) GFP-CDKL5 WT. For analysis of phosphorylated EB2, for each sample data were normalized to the total levels of corresponding protein. Data are expressed as mean ± SEM. * p < 0.05 by Kruskal-Wallis test followed by Dunn’s multiple comparison test.

    Journal: Scientific Reports

    Article Title: Engineered tRNAs efficiently suppress CDKL5 premature termination codons

    doi: 10.1038/s41598-024-82766-0

    Figure Lengend Snippet: Phosphorylation of the exogenous GFP-CDKL5 TEY motif and endogenous EB2 are restored following ACE-tRNA Arg UGA delivery. ( a , c ) Representative western blots for CDKL5 pTyr 171 ( a ) and EB2 pSer 222 ( c ) in HEK293T co-transfected with GFP, WT GFP-CDKL5 or -R59X, -R134X, -R550X constructs with or without 1X or 4X ACE-tRNA Arg UGA . CDKL5 phosphorylation at Tyr 171 was revealed together with full-length CDKL5 (through an anti-GFP antibody). White arrowheads show the premature truncated GFP-CDKL5 R550X derivative that was not subjected to readthrough. Original images of blots in a are shown in the Supplementary Information file. ( b , d ) Histograms depict the percentages of full-length GFP-CDKL5 phosphorylation at Tyr 171 ( b ) and of EB2 phosphorylation at Ser 222 ( d ) with respect to untreated (UT) GFP-CDKL5 WT. For analysis of phosphorylated EB2, for each sample data were normalized to the total levels of corresponding protein. Data are expressed as mean ± SEM. * p < 0.05 by Kruskal-Wallis test followed by Dunn’s multiple comparison test.

    Article Snippet: The following primary antibodies were used at 1:1000 dilution: mouse monoclonal anti-GFP (#11814460001, Roche Diagnostics), sheep polyclonal anti-CDKL5 phospho-Tyr 171 (MRC PPU Reagents and Services, University of Dundee, UK), sheep anti-MAP1S p-Ser 900 (MRC PPU Reagents and Services, University of Dundee, UK), rabbit polyclonal anti-EB2 phospho-Ser 222 (#00117741, Covalab) and rabbit polyclonal anti-EB2 (#orb234827, Biorbyt).

    Techniques: Western Blot, Transfection, Construct, Comparison