Journal: Cancers
Article Title: Characterization of the Tumor-Microenvironment in Patient-Derived Cervix Xenografts (OCICx)
doi: 10.3390/cancers4030821
Figure Lengend Snippet: Quantitation of the staining for the various markers in the tumor and stromal components separately in the 4 models (OCICx 1, 3, 8, 16) over passage number. The percent pixel positivity is shown for each marker ( A ) CA-9, EF5, CD31; ( B ) Ki67 and LYVE1. Values plotted are mean ± SD. Passage 0 indicates biopsy piece.
Article Snippet: The following antibodies and dilutions were used for immunohistochemistry: Rabbit anti-human polyclonal CA-9 (Novus Biologicals, Oakville, ON, Canada, NB100-417) 1:2000; Mouse anti-EF5 (Non commercial, gift from Cam Koch) 1:250; rabbit anti-mouse polyclonal CD31 (Santa Cruz, Santa Cruz, CA, USA), Sc-1506-R) 1:1000; anti-mouse polyclonal LYVE1 (rabbit) from Novus Biologicals (NB600-1008) 1:200; anti-human Ki67 (rabbit) from Neomarkers (Fremont, California, USA, M-9106-S1) 1:500.
Techniques: Quantitation Assay, Staining, Marker