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Santa Cruz Biotechnology rabbit igg anti caga polyclonal antibody b 300
Expression, delivery, and phosphorylation of <t>CagA.</t> (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the <t>polyclonal</t> anti-CagA antibody b-300 (bottom). (B)
Rabbit Igg Anti Caga Polyclonal Antibody B 300, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 873 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg anti caga polyclonal antibody b 300/product/Santa Cruz Biotechnology
Average 85 stars, based on 873 article reviews
Price from $9.99 to $1999.99
rabbit igg anti caga polyclonal antibody b 300 - by Bioz Stars, 2020-09
85/100 stars

Images

1) Product Images from "Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿ §"

Article Title: Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿ §

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.02330-08

Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)
Figure Legend Snippet: Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)

Techniques Used: Expressing, Western Blot

2) Product Images from "Dynamic Expansion and Contraction of cagA Copy Number in Helicobacter pylori Impact Development of Gastric Disease"

Article Title: Dynamic Expansion and Contraction of cagA Copy Number in Helicobacter pylori Impact Development of Gastric Disease

Journal: mBio

doi: 10.1128/mBio.01779-16

Relative levels of CagA protein and CagA phosphorylation. (A) The protein levels of CagA and UreA in lysates of H. pylori strains PMSS1, S F -1, S L -2, M F -3, and M L -1 were measured by Western blotting (upper panel). For each lysate, 0.5, 1, 1.5, and 2 μg of total protein were used to determine standard curves for CagA and UreA. The immunoblot images were analyzed using ImageJ software, and the values were plotted on a graph (lower panel). (B) Ratios of CagA to UreA were calculated, and each value was normalized to the value calculated for cagA -S F -1 to determine relative CagA protein levels. The bar graphs indicate average levels of CagA expression of each strain, and error bars represent standard deviations, derived from results of 2 independent experiments. (C) Lysates of AGS cells that were infected with H. pylori strains PMSS1, Δ cagA FL -2, S F -1, S L -2, M F -3, and M L -1 were immunoblotted for phosphorylated CagA (p-CagA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), CagA, and UreA. GAPDH and UreA were used as controls.
Figure Legend Snippet: Relative levels of CagA protein and CagA phosphorylation. (A) The protein levels of CagA and UreA in lysates of H. pylori strains PMSS1, S F -1, S L -2, M F -3, and M L -1 were measured by Western blotting (upper panel). For each lysate, 0.5, 1, 1.5, and 2 μg of total protein were used to determine standard curves for CagA and UreA. The immunoblot images were analyzed using ImageJ software, and the values were plotted on a graph (lower panel). (B) Ratios of CagA to UreA were calculated, and each value was normalized to the value calculated for cagA -S F -1 to determine relative CagA protein levels. The bar graphs indicate average levels of CagA expression of each strain, and error bars represent standard deviations, derived from results of 2 independent experiments. (C) Lysates of AGS cells that were infected with H. pylori strains PMSS1, Δ cagA FL -2, S F -1, S L -2, M F -3, and M L -1 were immunoblotted for phosphorylated CagA (p-CagA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), CagA, and UreA. GAPDH and UreA were used as controls.

Techniques Used: Western Blot, Software, Expressing, Derivative Assay, Infection

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Preservation of Helicobacter pylori CagA Translocation and Host Cell Proinflammatory Responses in the Face of CagL Hypervariability at Amino Acid Residues 58/59
Article Snippet: .. Spent culture media and cell lysates were harvested at 6 and 24 hours post-inoculation (hpi): spent media was analysed for IL-8 secretion using the human IL-8 ELISA set (BD Biosciences) and by immunoblot analysis of cell lysates for phosphorylated CagA using PY99 mouse monoclonal phosphotyrosine- and rabbit polyclonal CagA-specific antibodies (Santa Cruz Biotechnology) as previously described [ ]. .. PY99-specific signal was detected using Amersham ECL Prime chemiluminescent substrate (GE Healthcare) and CagA-specific signal was detected using LumiGLO chemiluminescent substrate (KPL).

SDS-Gel:

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori
Article Snippet: .. Proteins (20 μg) were separated by SDS-gel electrophoresis, amounts of CagA and phosphorylated-CagA were determined by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz) and anti-phosphotyrosine antibody PY99 (Santa Cruz). .. The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression.

Incubation:

Article Title: Pathogenic Acanthamoeba spp. Secrete a Mannose-Induced Cytolytic Protein That Correlates with the Ability To Cause Disease
Article Snippet: .. Rabbit anti-Chinese hamster IgA hyperimmune serum ( ) was then added to a 1:2 dilution and incubated for 2 h. Plates were washed, and a 1:1,000 dilution of goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology) was added. .. Plates were developed by adding 1.0 mM 2,2′-azinobis(3-ethyl-benzthiazoline-6-sulfonic acid) (Sigma) containing 0.003% H2 O2 and incubated for 30 min at room temperature.

Article Title: LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis
Article Snippet: .. The membrane was washed with TBS-0.5% Tween-20 and incubated with goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology), at a dilution of 1/400, for 90 min at 24°C. .. Surface LAM staining Mid-log shaking cultures were centrifuged at 800 rpm for 8 min to remove clumps.

Article Title: Staphylococcus aureus Extracellular Vesicles Carry Biologically Active ?-Lactamase
Article Snippet: .. The blocked membrane was incubated with the lab-made polyclonal anti-BlaZ antibody and then incubated with goat anti-rabbit IgG-horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA). ..

Electrophoresis:

Article Title: Fragmentation of CagA Reduces Hummingbird Phenotype Induction by Helicobactor pylori
Article Snippet: .. Proteins (20 μg) were separated by SDS-gel electrophoresis, amounts of CagA and phosphorylated-CagA were determined by immunoblotting using polyclonal anti-CagA antibody b-300 (Santa Cruz) and anti-phosphotyrosine antibody PY99 (Santa Cruz). .. The H . pylori 60190 and 26695 cagA knockout mutant (26695ΔA) were included as a positive and negative control, respectively, for CagA expression.

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    Santa Cruz Biotechnology rabbit igg anti caga polyclonal antibody b 300
    Expression, delivery, and phosphorylation of <t>CagA.</t> (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the <t>polyclonal</t> anti-CagA antibody b-300 (bottom). (B)
    Rabbit Igg Anti Caga Polyclonal Antibody B 300, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit igg anti caga polyclonal antibody b 300/product/Santa Cruz Biotechnology
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit igg anti caga polyclonal antibody b 300 - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)

    Journal: Journal of Clinical Microbiology

    Article Title: Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿Polymorphism in the CagA EPIYA Motif Impacts Development of Gastric Cancer ▿ §

    doi: 10.1128/JCM.02330-08

    Figure Lengend Snippet: Expression, delivery, and phosphorylation of CagA. (A) Western blot analysis of bacterial lysates from the six indicated Korean strains was conducted using a monoclonal anti-CagA antibody (top) or the polyclonal anti-CagA antibody b-300 (bottom). (B)

    Article Snippet: Alternatively, membranes were probed with a 1:5,000 dilution of rabbit IgG anti-CagA polyclonal antibody b-300 (Santa Cruz Biotechnology), followed by a 1:20,000 dilution of HRP-conjugated bovine anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology).

    Techniques: Expressing, Western Blot