rabbit polyclonal ab  (Abcam)

 
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  • 99
    Name:
    Anti CDX2 antibody
    Description:

    Catalog Number:
    ab88129
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    Structured Review

    Abcam rabbit polyclonal ab
    CDX2 immunolabelling controls. The binding specificity of the rabbit <t>polyclonal</t> anti CDX2 primary antibody (ab88129) (a) and mouse monoclonal anti CDX2 primary antibody (ab15258) (b) was verified with the use of blocking peptide (ab99158) prior to the antibody staining. The secondary antibodies used were goat anti-rabbit Rhodamine conjugated (sc-2091) in (a) and goat anti-mouse Rhodamine conjugated (sc-2092) in (b) . Dashed circle marks the metaphase plate. DAPI labels chromatin.

    https://www.bioz.com/result/rabbit polyclonal ab/product/Abcam
    Average 99 stars, based on 60 article reviews
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    rabbit polyclonal ab - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development"

    Article Title: Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development

    Journal: BMC Developmental Biology

    doi: 10.1186/1471-213X-13-32

    CDX2 immunolabelling controls. The binding specificity of the rabbit polyclonal anti CDX2 primary antibody (ab88129) (a) and mouse monoclonal anti CDX2 primary antibody (ab15258) (b) was verified with the use of blocking peptide (ab99158) prior to the antibody staining. The secondary antibodies used were goat anti-rabbit Rhodamine conjugated (sc-2091) in (a) and goat anti-mouse Rhodamine conjugated (sc-2092) in (b) . Dashed circle marks the metaphase plate. DAPI labels chromatin.
    Figure Legend Snippet: CDX2 immunolabelling controls. The binding specificity of the rabbit polyclonal anti CDX2 primary antibody (ab88129) (a) and mouse monoclonal anti CDX2 primary antibody (ab15258) (b) was verified with the use of blocking peptide (ab99158) prior to the antibody staining. The secondary antibodies used were goat anti-rabbit Rhodamine conjugated (sc-2091) in (a) and goat anti-mouse Rhodamine conjugated (sc-2092) in (b) . Dashed circle marks the metaphase plate. DAPI labels chromatin.

    Techniques Used: Binding Assay, Blocking Assay, Staining

    OCT4 immunolabelling controls. Control immunostaining with both of the anti OCT4 antibodies used in the experiment. (a1) presents HBL single labelled for OCT4 with goat polyclonal primary antibody (ab27985) detected with mouse anti-goat Rhodamine conjugated secondary antibody (sc-24900). (a2) presents single labelled HBl with rabbit polyclonal anti OCT4 antibody (ab18976) detected with goat anti-rabbit FITC conjugated secondary antibody (sc-2012). (b) indicates colocalisation of the OCT4 in HBl double-labelled with both of the anti OCT4 antibodies. The secondary antibodies used were mouse anti-goat Rhodamine conjugated (sc-2490) and donkey anti-rabbit FITC conjugated (sc-2090). DAPI labels chromatin.
    Figure Legend Snippet: OCT4 immunolabelling controls. Control immunostaining with both of the anti OCT4 antibodies used in the experiment. (a1) presents HBL single labelled for OCT4 with goat polyclonal primary antibody (ab27985) detected with mouse anti-goat Rhodamine conjugated secondary antibody (sc-24900). (a2) presents single labelled HBl with rabbit polyclonal anti OCT4 antibody (ab18976) detected with goat anti-rabbit FITC conjugated secondary antibody (sc-2012). (b) indicates colocalisation of the OCT4 in HBl double-labelled with both of the anti OCT4 antibodies. The secondary antibodies used were mouse anti-goat Rhodamine conjugated (sc-2490) and donkey anti-rabbit FITC conjugated (sc-2090). DAPI labels chromatin.

    Techniques Used: Immunostaining

    2) Product Images from "Malaria transmission assisted by interaction between Plasmodium α-tubulin-1 and Anopheles FREP1 protein"

    Article Title: Malaria transmission assisted by interaction between Plasmodium α-tubulin-1 and Anopheles FREP1 protein

    Journal: bioRxiv

    doi: 10.1101/2019.12.16.878082

    Protein sequence comparison and purified Ab transmission-blocking assays. A ) Sequence alignment of human α-tubulin (HA), P. falciparum α-tubulin-1 (PfA), human β-tubulin (HB), P. falciparum β-tubulin (PfB). B ) Purified rabbit anti-human α-tubulin polyclonal Ab (labeled with α) significantly reduced the number of P. falciparum oocysts in An. gambiae midguts, while purified Ab against β-tubulin (labeled with β) did not inhibit P. falciparum transmission to An. gambiae. A non-related purified rabbit polyclonal Ab (anti-V5, labeled with control) was used as the negative control. Wilcoxon test was used to calculated P-value. The experiment was repeated twice and the results were similar.
    Figure Legend Snippet: Protein sequence comparison and purified Ab transmission-blocking assays. A ) Sequence alignment of human α-tubulin (HA), P. falciparum α-tubulin-1 (PfA), human β-tubulin (HB), P. falciparum β-tubulin (PfB). B ) Purified rabbit anti-human α-tubulin polyclonal Ab (labeled with α) significantly reduced the number of P. falciparum oocysts in An. gambiae midguts, while purified Ab against β-tubulin (labeled with β) did not inhibit P. falciparum transmission to An. gambiae. A non-related purified rabbit polyclonal Ab (anti-V5, labeled with control) was used as the negative control. Wilcoxon test was used to calculated P-value. The experiment was repeated twice and the results were similar.

    Techniques Used: Sequencing, Purification, Transmission Assay, Blocking Assay, Labeling, Negative Control

    Immunofluorescence assay showed that anti-human α-tubulin Ab bound to the impermeable-fixed P. falciparum ookinetes at their apical end. A ) Negative control. P. falciparum ookinetes stained by an un-related polyclonal Ab (ant-V5 AB). No binding signals were detected. B ) Impermeable ookinetes stained by the anti-human-α-tubulin Ab. Ab bound to the apical end of a paraformaldehyde-fixed ookinete. In each treatment, the 1 st row detected α-tubulin-1 (red color). The 2 nd showed the co-localization of P. falciparum (nuclei, blue color) and α-tubulin-1. The 3 rd column shows the bright views of the cells. The white arrows point to ookinetes. The green arrow points to a gametocyte.
    Figure Legend Snippet: Immunofluorescence assay showed that anti-human α-tubulin Ab bound to the impermeable-fixed P. falciparum ookinetes at their apical end. A ) Negative control. P. falciparum ookinetes stained by an un-related polyclonal Ab (ant-V5 AB). No binding signals were detected. B ) Impermeable ookinetes stained by the anti-human-α-tubulin Ab. Ab bound to the apical end of a paraformaldehyde-fixed ookinete. In each treatment, the 1 st row detected α-tubulin-1 (red color). The 2 nd showed the co-localization of P. falciparum (nuclei, blue color) and α-tubulin-1. The 3 rd column shows the bright views of the cells. The white arrows point to ookinetes. The green arrow points to a gametocyte.

    Techniques Used: Immunofluorescence, Negative Control, Staining, Binding Assay

    3) Product Images from "Comparison of rhinovirus A infection in human primary epithelial and HeLa cells"

    Article Title: Comparison of rhinovirus A infection in human primary epithelial and HeLa cells

    Journal: The Journal of General Virology

    doi: 10.1099/vir.0.031302-0

    Viral antibody reactivity. Rabbit polyclonal sera developed against 2A pro (a), 3C pro (b) and 3D pol (c) from HRV A16 were reacted in Western assays with HeLa cell lysates infected (10 p.f.u. per cell) with the indicated HRV and then harvested at 18 h p.i.
    Figure Legend Snippet: Viral antibody reactivity. Rabbit polyclonal sera developed against 2A pro (a), 3C pro (b) and 3D pol (c) from HRV A16 were reacted in Western assays with HeLa cell lysates infected (10 p.f.u. per cell) with the indicated HRV and then harvested at 18 h p.i.

    Techniques Used: Western Blot, Infection

    Related Articles

    Western Blot:

    Article Title: Mechanisms of transcription factor-mediated direct reprogramming of mouse embryonic stem cells to trophoblast stem-like cells
    Article Snippet: .. Antibodies used for western blotting were anti-Cdx2 (1:1000, ab88129, Abcam), anti-Arid3a (1:5000; ( )), anti-Gata3 (1:1000, SC-9009, Santa Cruz), anti-β-actin (1:10000, ab20272, Abcam) and anti-Hdac1 (1:1000, ab7028, Abcam). .. Co-immunoprecipitation One-step affinity purification with streptavidin-agarose beads (Invitrogen) using ∼2 × 107 cells from ES cell lines expressing BirA only or BirA plus biotin-tagged proteins was performed as previously described ( ).

    Article Title: Hematopoietic stem and progenitor cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia
    Article Snippet: .. Western blot Immunoblot for Scl -CreERT and Scl:Cdx2 cells was performed using mouse anti-CDX2 monoclonal antibody (CDX-88; Abcam) (1:1000) and mouse anti-β-actin (BD Biosciences) (1:5000). .. Immunoblot for rabbit anti-Flag immunoprecipitation of Ba/F3 cells transduced with pMIG-Cdx2-Flag was performed using mouse anti-Flag tag monoclonal antibody (8146, Cell Signaling) (1:1000).

    Article Title: Identification and characterisation of NANOG+/ OCT-4high/SOX2+ doxorubicin-resistant stem-like cells from transformed trophoblastic cell lines
    Article Snippet: .. Antibodies For this study, we used anti-OCT4 (1:200 for IF, 1:500 for WB, ab18976 Abcam), anti- SOX2 (1:500 for IF, 1:1000 for WB, ab97959 Abcam), anti-NANOG (1:50 for IF, 1:500 for WB, OAAB11202, Aviva Systems Biology), anti-CDX2 (1:100 for IF, ab15258 Abcam; 1:500 for WB, ab88129 Abcam), anti-beta actin (1:1000 for WB, ab8227 Abcam) and Anti-LASP1 (1:1000 for WB, ab156872 Abcam). ..

    Incubation:

    Article Title: HpSlyD inducing CDX2 and VIL1 expression mediated through TCTP protein may contribute to intestinal metaplasia in the stomach
    Article Snippet: .. The membranes were incubated with primary antibodies overnight at 4 °C: rabbit monoclonal anti-CDX2 (1:2000, Abcam, USA), mouse monoclonal anti-VIL1 (1:2000, Origene, USA), rabbit monoclonal anti-TCTP (1:250, Abcam, USA) and then with the appropriate horseradish peroxidase-conjugated secondary antibody (Zhongshan Golden Bridge Biotechnology Co. Ltd, Beijing, China) and visualized by enhanced chemiluminescence (Solarbio, China). .. TCTP RNA interference and overexpression A small interfering RNA (siRNA) duplex targeting TCTP (5′-GAAATCAATCAAAGGGAAA-3′) and a nonsilencing control siRNA duplex were synthesized by RIBOBIO (Guangzhou, China).

    other:

    Article Title: MicroRNA-181d-5p-Containing Exosomes Derived from CAFs Promote EMT by Regulating CDX2/HOXA5 in Breast Cancer
    Article Snippet: Next, the sections were blocked with normal goat serum (C-0005; Shanghai Haoran Biological Technology, Shanghai, China) at room temperature for 20 min. Later, the sections were probed with primary antibody rabbit anti-CDX2 (ab88129, 1:100) and HOXA5 (ab82645, 1:100) at 4°C overnight and then with secondary antibody goat anti-rabbit IgG (ab6785, 1:1,000) at 37°C for 20 min.

    Labeling:

    Article Title: Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression
    Article Snippet: .. Subsequently, the membrane was hybridized with primary rabbit anti-human antibodies to CDX2 (dilution ratio of 1:2000, ab88129), COL11A1 (dilution ratio of 1:1000, ab64883), E-cadherin (dilution ratio of 1:500, ab15148), Vimentin (dilution ratio of 1:2500, ab92547) and GAPDH (dilution ratio of 1:2500, ab9485) and with the goat anti-rabbit IgG labeled by HRP (dilution ratio of 1:20,000, ab205718). ..

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  • 91
    Abcam rabbit anti ct45a1
    <t>CT45A1</t> levels in WI-38 normal lung cells and the lung cancer cell lines, 95-D, H1299, A549, NCI-H292 and NCI-H1650. The levels were determined by western blotting. CT45A1, cancer-testis antigen family 45 member A1.
    Rabbit Anti Ct45a1, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ct45a1/product/Abcam
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Abcam rabbit polyclonal anti cap d3
    The interaction between KIF4A and condensin I. (A) HeLa cells expressing EGFP-KIF4A WT or S1186 were arrested at mitosis and the KIF4A-condensin I complex was recovered by immunoprecipitation against GFP and SMC2. Mitotic extracts prepared from no EGFP expressing cells (-) were also analyzed as a negative control. To detect KIF4A and condensin, the anti-GFP, -CAPD-2, <t>-CAP-D3,</t> and -SMC2 antibodies were used for WB analysis. (B) A model of the chromosome organization by KIF4A phosphorylation. In interphase, KIF4A and condensin I localize in the nucleus and cytoplasm, respectively. In early mitosis, KIF4A is phosphorylated at S1186 by Cdk1, and associates with the chromosome. After NEB, condensin I is also distributed to the chromosome. KIF4A and condensin I then would form a complex in the chromosome by their further phosphorylation by aurora B. KIF4A assists in the accumulation of condensin I in the chromosome scaffold using its motor activity, and lateral chromatid compaction is achieved.
    Rabbit Polyclonal Anti Cap D3, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam anti nucb2 antibodies
    Downregulated expression of <t>NUCB2</t> inhibited the migration and invasion of thyroid cancer cells by regulating MMP2 and MMP9. Notes: ( A ) Transwell assay demonstrated that the ability of invasion in shRNA group was significantly decreased comparing to control group (n=3, P
    Anti Nucb2 Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Abcam anti asialo gm1
    Domain formation in B. burgdorferi as determined by immunogold TEM analysis requires raft-supporting sterols. A. Representative negative-stain TEM images of B. burgdorferi substituted with the indicated sterols and probed for sterol glycolipids using a rabbit antibody to <t>asialo-GM1</t> followed by a secondary anti-rabbit antibody conjugated to 6 nm colloidal gold. Micrographs show electron dense regions, which are portions of B. burgdorferi and show associated gold particles. Two images (of about 400 nm long segments) are shown for each sterol. Top row, sterols strongly supporting ordered domain formation; middle row, sterols with an intermediate ability to form ordered domains; bottom row, sterols that inhibit ordered domain formation [22] , [26] – [28] . Boxes highlight sterol glycolipid clusters. Bars = 100 nm. TEM micrographs for additional sterols are shown in Fig. S3 in Text S1 . B. Pooled “K-function” analysis of TEM experiments. The spatial distribution of gold particles is presented as curves representing the mean values of L(r)-r from images of three different bacteria from three independent sterol substitution experiments. Values of L(r)-r above the CI (95% confidence level, dashed line) indicate clustering (i.e. domain formation) of the sterol glycolipid at that specific length scale.
    Anti Asialo Gm1, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CT45A1 levels in WI-38 normal lung cells and the lung cancer cell lines, 95-D, H1299, A549, NCI-H292 and NCI-H1650. The levels were determined by western blotting. CT45A1, cancer-testis antigen family 45 member A1.

    Journal: Molecular Medicine Reports

    Article Title: CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway

    doi: 10.3892/mmr.2017.7466

    Figure Lengend Snippet: CT45A1 levels in WI-38 normal lung cells and the lung cancer cell lines, 95-D, H1299, A549, NCI-H292 and NCI-H1650. The levels were determined by western blotting. CT45A1, cancer-testis antigen family 45 member A1.

    Article Snippet: Rabbit anti-CT45A1 (cat. no. ab170339) polyclonal antibody was purchased from Abcam.

    Techniques: Western Blot

    Viability of A549 cells transfected with CT45A1 siRNA and negative control was determined by an MTT assay. **P

    Journal: Molecular Medicine Reports

    Article Title: CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway

    doi: 10.3892/mmr.2017.7466

    Figure Lengend Snippet: Viability of A549 cells transfected with CT45A1 siRNA and negative control was determined by an MTT assay. **P

    Article Snippet: Rabbit anti-CT45A1 (cat. no. ab170339) polyclonal antibody was purchased from Abcam.

    Techniques: Transfection, Negative Control, MTT Assay

    Micrographs of A549 cells transfected with CT45A1 siRNA and negative control in the lower chambers of a Transwell assay, for the assessment of cell (A) migration and (B) invasion. Magnification, ×200. CT45A1, cancer-testis antigen family 45 member A1; siRNA, small interfering RNA.

    Journal: Molecular Medicine Reports

    Article Title: CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway

    doi: 10.3892/mmr.2017.7466

    Figure Lengend Snippet: Micrographs of A549 cells transfected with CT45A1 siRNA and negative control in the lower chambers of a Transwell assay, for the assessment of cell (A) migration and (B) invasion. Magnification, ×200. CT45A1, cancer-testis antigen family 45 member A1; siRNA, small interfering RNA.

    Article Snippet: Rabbit anti-CT45A1 (cat. no. ab170339) polyclonal antibody was purchased from Abcam.

    Techniques: Transfection, Negative Control, Transwell Assay, Migration, Small Interfering RNA

    Western blotting analysis of (A) Bax, Bcl-2 and survivin, (B) MMP2 and MMP9 and (C) ERK/CREB in A549 cells transfected with CT45A1 siRNA and negative control. **P

    Journal: Molecular Medicine Reports

    Article Title: CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway

    doi: 10.3892/mmr.2017.7466

    Figure Lengend Snippet: Western blotting analysis of (A) Bax, Bcl-2 and survivin, (B) MMP2 and MMP9 and (C) ERK/CREB in A549 cells transfected with CT45A1 siRNA and negative control. **P

    Article Snippet: Rabbit anti-CT45A1 (cat. no. ab170339) polyclonal antibody was purchased from Abcam.

    Techniques: Western Blot, Transfection, Negative Control

    Apoptosis in A549 cells transfected with CT45A1 siRNA and negative control was determined by Annexin V-FITC/propidium iodide staining and flow cytometry. CT45A1, cancer-testis antigen family 45 member A1; siRNA, small interfering RNA; FITC, fluorescein isothiocyanate.

    Journal: Molecular Medicine Reports

    Article Title: CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway

    doi: 10.3892/mmr.2017.7466

    Figure Lengend Snippet: Apoptosis in A549 cells transfected with CT45A1 siRNA and negative control was determined by Annexin V-FITC/propidium iodide staining and flow cytometry. CT45A1, cancer-testis antigen family 45 member A1; siRNA, small interfering RNA; FITC, fluorescein isothiocyanate.

    Article Snippet: Rabbit anti-CT45A1 (cat. no. ab170339) polyclonal antibody was purchased from Abcam.

    Techniques: Transfection, Negative Control, Staining, Flow Cytometry, Cytometry, Small Interfering RNA

    CT45A1 mRNA and protein levels in A549 cells transfected with CT45A1 siRNA and negative control were determined by semi-quantitative reverse transcription polymerase chain reaction (left panel) and western blotting (right panel), respectively. **P

    Journal: Molecular Medicine Reports

    Article Title: CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway

    doi: 10.3892/mmr.2017.7466

    Figure Lengend Snippet: CT45A1 mRNA and protein levels in A549 cells transfected with CT45A1 siRNA and negative control were determined by semi-quantitative reverse transcription polymerase chain reaction (left panel) and western blotting (right panel), respectively. **P

    Article Snippet: Rabbit anti-CT45A1 (cat. no. ab170339) polyclonal antibody was purchased from Abcam.

    Techniques: Transfection, Negative Control, Reverse Transcription Polymerase Chain Reaction, Western Blot

    The interaction between KIF4A and condensin I. (A) HeLa cells expressing EGFP-KIF4A WT or S1186 were arrested at mitosis and the KIF4A-condensin I complex was recovered by immunoprecipitation against GFP and SMC2. Mitotic extracts prepared from no EGFP expressing cells (-) were also analyzed as a negative control. To detect KIF4A and condensin, the anti-GFP, -CAPD-2, -CAP-D3, and -SMC2 antibodies were used for WB analysis. (B) A model of the chromosome organization by KIF4A phosphorylation. In interphase, KIF4A and condensin I localize in the nucleus and cytoplasm, respectively. In early mitosis, KIF4A is phosphorylated at S1186 by Cdk1, and associates with the chromosome. After NEB, condensin I is also distributed to the chromosome. KIF4A and condensin I then would form a complex in the chromosome by their further phosphorylation by aurora B. KIF4A assists in the accumulation of condensin I in the chromosome scaffold using its motor activity, and lateral chromatid compaction is achieved.

    Journal: PLoS ONE

    Article Title: Cdk1-dependent phosphorylation of KIF4A at S1186 triggers lateral chromosome compaction during early mitosis

    doi: 10.1371/journal.pone.0209614

    Figure Lengend Snippet: The interaction between KIF4A and condensin I. (A) HeLa cells expressing EGFP-KIF4A WT or S1186 were arrested at mitosis and the KIF4A-condensin I complex was recovered by immunoprecipitation against GFP and SMC2. Mitotic extracts prepared from no EGFP expressing cells (-) were also analyzed as a negative control. To detect KIF4A and condensin, the anti-GFP, -CAPD-2, -CAP-D3, and -SMC2 antibodies were used for WB analysis. (B) A model of the chromosome organization by KIF4A phosphorylation. In interphase, KIF4A and condensin I localize in the nucleus and cytoplasm, respectively. In early mitosis, KIF4A is phosphorylated at S1186 by Cdk1, and associates with the chromosome. After NEB, condensin I is also distributed to the chromosome. KIF4A and condensin I then would form a complex in the chromosome by their further phosphorylation by aurora B. KIF4A assists in the accumulation of condensin I in the chromosome scaffold using its motor activity, and lateral chromatid compaction is achieved.

    Article Snippet: Antibodies The following primary antibodies were used for immunostaining (IF) and western blotting (WB): rabbit polyclonal anti-KIF4A (IF-1:500, WB-1:500; Thermo), rabbit polyclonal anti-phosphorylated KIF4A S1186 (WB-1:500), rabbit polyclonal anti-CAP-H1 (IF-1:100, WB-1:300), rabbit polyclonal anti-GFP (WB-1:1000; Thermo), rabbit polyclonal anti-CAP-H2 (WB-1:500), rat polyclonal anti-CAP-H2 (IF-1:500; Cosmo bio), rabbit polyclonal anti-CAP-D2 (WB-1:500), rabbit polyclonal anti-CAP-D3 (WB-1:500), rabbit polyclonal anti-histone H3S10 phosphorylation (WB-1:2000; Upstate), chicken polyclonal anti-GFP (IF-1:1000; Abcam), rabbit polyclonal anti-histone H3 (WB-1:1000; Millipore), and mouse monoclonal anti-α-tubulin (WB-1:1000; Calbiochem).

    Techniques: Expressing, Immunoprecipitation, Negative Control, Western Blot, Activity Assay

    Downregulated expression of NUCB2 inhibited the migration and invasion of thyroid cancer cells by regulating MMP2 and MMP9. Notes: ( A ) Transwell assay demonstrated that the ability of invasion in shRNA group was significantly decreased comparing to control group (n=3, P

    Journal: OncoTargets and therapy

    Article Title: High expression of NUCB2 promotes papillary thyroid cancer cells proliferation and invasion

    doi: 10.2147/OTT.S184560

    Figure Lengend Snippet: Downregulated expression of NUCB2 inhibited the migration and invasion of thyroid cancer cells by regulating MMP2 and MMP9. Notes: ( A ) Transwell assay demonstrated that the ability of invasion in shRNA group was significantly decreased comparing to control group (n=3, P

    Article Snippet: The tissue sections were treated with anti-NUCB2 antibodies (rabbit polyclonal antibody to NUCB2, ab224348; Abcam, Cambridge, UK) at 1:1,000 dilution and incubated overnight at 4°C.

    Techniques: Expressing, Migration, Transwell Assay, shRNA

    The influence of NUCB2 on tumor growth of thyroid cancer in mice. Notes: ( A ) Representative images of tumors from both groups are shown. Tumor volumes of shRNA group were smaller than those of the control group (n=6, P

    Journal: OncoTargets and therapy

    Article Title: High expression of NUCB2 promotes papillary thyroid cancer cells proliferation and invasion

    doi: 10.2147/OTT.S184560

    Figure Lengend Snippet: The influence of NUCB2 on tumor growth of thyroid cancer in mice. Notes: ( A ) Representative images of tumors from both groups are shown. Tumor volumes of shRNA group were smaller than those of the control group (n=6, P

    Article Snippet: The tissue sections were treated with anti-NUCB2 antibodies (rabbit polyclonal antibody to NUCB2, ab224348; Abcam, Cambridge, UK) at 1:1,000 dilution and incubated overnight at 4°C.

    Techniques: Mouse Assay, shRNA

    The expression of NUCB2 in human thyroid cancer and in the tissues adjacent to cancer. Notes: ( A ) Represents immunohistochemical images of tissues showing high and low NUCB2 expression in human thyroid cancer at 100× and 200× optical magnifications. ( B ) Immunohistochemical images of NUCB2 expression in human tissues adjacent to cancer tissue at 100× and 200× optical magnifications.

    Journal: OncoTargets and therapy

    Article Title: High expression of NUCB2 promotes papillary thyroid cancer cells proliferation and invasion

    doi: 10.2147/OTT.S184560

    Figure Lengend Snippet: The expression of NUCB2 in human thyroid cancer and in the tissues adjacent to cancer. Notes: ( A ) Represents immunohistochemical images of tissues showing high and low NUCB2 expression in human thyroid cancer at 100× and 200× optical magnifications. ( B ) Immunohistochemical images of NUCB2 expression in human tissues adjacent to cancer tissue at 100× and 200× optical magnifications.

    Article Snippet: The tissue sections were treated with anti-NUCB2 antibodies (rabbit polyclonal antibody to NUCB2, ab224348; Abcam, Cambridge, UK) at 1:1,000 dilution and incubated overnight at 4°C.

    Techniques: Expressing, Immunohistochemistry

    Knocking down of NUCB2 by shRNA in both TPC-1 and K1 cells. Notes: RT-PCR ( A ) and Western blot ( B ) showed lower expression level of NUCB2 in shRNA group (n=3, P

    Journal: OncoTargets and therapy

    Article Title: High expression of NUCB2 promotes papillary thyroid cancer cells proliferation and invasion

    doi: 10.2147/OTT.S184560

    Figure Lengend Snippet: Knocking down of NUCB2 by shRNA in both TPC-1 and K1 cells. Notes: RT-PCR ( A ) and Western blot ( B ) showed lower expression level of NUCB2 in shRNA group (n=3, P

    Article Snippet: The tissue sections were treated with anti-NUCB2 antibodies (rabbit polyclonal antibody to NUCB2, ab224348; Abcam, Cambridge, UK) at 1:1,000 dilution and incubated overnight at 4°C.

    Techniques: shRNA, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing

    Knocking down NUCB2 in papillary thyroid cancer cells inhibited tumor cell proliferation by regulating Ki67 and PCNA. Notes: ( A ) Typical images of colony-forming assay and its quantification demonstrated that colony rate of shRNA group was significantly lower than control group (n=3, P

    Journal: OncoTargets and therapy

    Article Title: High expression of NUCB2 promotes papillary thyroid cancer cells proliferation and invasion

    doi: 10.2147/OTT.S184560

    Figure Lengend Snippet: Knocking down NUCB2 in papillary thyroid cancer cells inhibited tumor cell proliferation by regulating Ki67 and PCNA. Notes: ( A ) Typical images of colony-forming assay and its quantification demonstrated that colony rate of shRNA group was significantly lower than control group (n=3, P

    Article Snippet: The tissue sections were treated with anti-NUCB2 antibodies (rabbit polyclonal antibody to NUCB2, ab224348; Abcam, Cambridge, UK) at 1:1,000 dilution and incubated overnight at 4°C.

    Techniques: shRNA

    Domain formation in B. burgdorferi as determined by immunogold TEM analysis requires raft-supporting sterols. A. Representative negative-stain TEM images of B. burgdorferi substituted with the indicated sterols and probed for sterol glycolipids using a rabbit antibody to asialo-GM1 followed by a secondary anti-rabbit antibody conjugated to 6 nm colloidal gold. Micrographs show electron dense regions, which are portions of B. burgdorferi and show associated gold particles. Two images (of about 400 nm long segments) are shown for each sterol. Top row, sterols strongly supporting ordered domain formation; middle row, sterols with an intermediate ability to form ordered domains; bottom row, sterols that inhibit ordered domain formation [22] , [26] – [28] . Boxes highlight sterol glycolipid clusters. Bars = 100 nm. TEM micrographs for additional sterols are shown in Fig. S3 in Text S1 . B. Pooled “K-function” analysis of TEM experiments. The spatial distribution of gold particles is presented as curves representing the mean values of L(r)-r from images of three different bacteria from three independent sterol substitution experiments. Values of L(r)-r above the CI (95% confidence level, dashed line) indicate clustering (i.e. domain formation) of the sterol glycolipid at that specific length scale.

    Journal: PLoS Pathogens

    Article Title: Proving Lipid Rafts Exist: Membrane Domains in the Prokaryote Borrelia burgdorferi Have the Same Properties as Eukaryotic Lipid Rafts

    doi: 10.1371/journal.ppat.1003353

    Figure Lengend Snippet: Domain formation in B. burgdorferi as determined by immunogold TEM analysis requires raft-supporting sterols. A. Representative negative-stain TEM images of B. burgdorferi substituted with the indicated sterols and probed for sterol glycolipids using a rabbit antibody to asialo-GM1 followed by a secondary anti-rabbit antibody conjugated to 6 nm colloidal gold. Micrographs show electron dense regions, which are portions of B. burgdorferi and show associated gold particles. Two images (of about 400 nm long segments) are shown for each sterol. Top row, sterols strongly supporting ordered domain formation; middle row, sterols with an intermediate ability to form ordered domains; bottom row, sterols that inhibit ordered domain formation [22] , [26] – [28] . Boxes highlight sterol glycolipid clusters. Bars = 100 nm. TEM micrographs for additional sterols are shown in Fig. S3 in Text S1 . B. Pooled “K-function” analysis of TEM experiments. The spatial distribution of gold particles is presented as curves representing the mean values of L(r)-r from images of three different bacteria from three independent sterol substitution experiments. Values of L(r)-r above the CI (95% confidence level, dashed line) indicate clustering (i.e. domain formation) of the sterol glycolipid at that specific length scale.

    Article Snippet: For slot blots, fractions were loaded onto nitrocellulose and probed with anti-asialo GM1 (for sterol glycolipids, rabbit polyclonal IgG, AbCam) or monoclonal antibodies CB2 (anti-OspB, mouse IgG) or CB10 (anti-OspA, mouse IgG) , .

    Techniques: Transmission Electron Microscopy, Staining