Anti CDX2 antibody
https://www.bioz.com/result/rabbit polyclonal ab/product/Abcam
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1) Product Images from "Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development"
Article Title: Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development
Journal: BMC Developmental Biology
Figure Legend Snippet: CDX2 immunolabelling controls. The binding specificity of the rabbit polyclonal anti CDX2 primary antibody (ab88129) (a) and mouse monoclonal anti CDX2 primary antibody (ab15258) (b) was verified with the use of blocking peptide (ab99158) prior to the antibody staining. The secondary antibodies used were goat anti-rabbit Rhodamine conjugated (sc-2091) in (a) and goat anti-mouse Rhodamine conjugated (sc-2092) in (b) . Dashed circle marks the metaphase plate. DAPI labels chromatin.
Techniques Used: Binding Assay, Blocking Assay, Staining
Figure Legend Snippet: OCT4 immunolabelling controls. Control immunostaining with both of the anti OCT4 antibodies used in the experiment. (a1) presents HBL single labelled for OCT4 with goat polyclonal primary antibody (ab27985) detected with mouse anti-goat Rhodamine conjugated secondary antibody (sc-24900). (a2) presents single labelled HBl with rabbit polyclonal anti OCT4 antibody (ab18976) detected with goat anti-rabbit FITC conjugated secondary antibody (sc-2012). (b) indicates colocalisation of the OCT4 in HBl double-labelled with both of the anti OCT4 antibodies. The secondary antibodies used were mouse anti-goat Rhodamine conjugated (sc-2490) and donkey anti-rabbit FITC conjugated (sc-2090). DAPI labels chromatin.
Techniques Used: Immunostaining
2) Product Images from "Malaria transmission assisted by interaction between Plasmodium α-tubulin-1 and Anopheles FREP1 protein"
Article Title: Malaria transmission assisted by interaction between Plasmodium α-tubulin-1 and Anopheles FREP1 protein
Figure Legend Snippet: Protein sequence comparison and purified Ab transmission-blocking assays. A ) Sequence alignment of human α-tubulin (HA), P. falciparum α-tubulin-1 (PfA), human β-tubulin (HB), P. falciparum β-tubulin (PfB). B ) Purified rabbit anti-human α-tubulin polyclonal Ab (labeled with α) significantly reduced the number of P. falciparum oocysts in An. gambiae midguts, while purified Ab against β-tubulin (labeled with β) did not inhibit P. falciparum transmission to An. gambiae. A non-related purified rabbit polyclonal Ab (anti-V5, labeled with control) was used as the negative control. Wilcoxon test was used to calculated P-value. The experiment was repeated twice and the results were similar.
Techniques Used: Sequencing, Purification, Transmission Assay, Blocking Assay, Labeling, Negative Control
Figure Legend Snippet: Immunofluorescence assay showed that anti-human α-tubulin Ab bound to the impermeable-fixed P. falciparum ookinetes at their apical end. A ) Negative control. P. falciparum ookinetes stained by an un-related polyclonal Ab (ant-V5 AB). No binding signals were detected. B ) Impermeable ookinetes stained by the anti-human-α-tubulin Ab. Ab bound to the apical end of a paraformaldehyde-fixed ookinete. In each treatment, the 1 st row detected α-tubulin-1 (red color). The 2 nd showed the co-localization of P. falciparum (nuclei, blue color) and α-tubulin-1. The 3 rd column shows the bright views of the cells. The white arrows point to ookinetes. The green arrow points to a gametocyte.
Techniques Used: Immunofluorescence, Negative Control, Staining, Binding Assay
3) Product Images from "Comparison of rhinovirus A infection in human primary epithelial and HeLa cells"
Article Title: Comparison of rhinovirus A infection in human primary epithelial and HeLa cells
Journal: The Journal of General Virology
Figure Legend Snippet: Viral antibody reactivity. Rabbit polyclonal sera developed against 2A pro (a), 3C pro (b) and 3D pol (c) from HRV A16 were reacted in Western assays with HeLa cell lysates infected (10 p.f.u. per cell) with the indicated HRV and then harvested at 18 h p.i.
Techniques Used: Western Blot, Infection
Article Title: Mechanisms of transcription factor-mediated direct reprogramming of mouse embryonic stem cells to trophoblast stem-like cells
Article Snippet: .. Antibodies used for western blotting were
Article Title: Hematopoietic stem and progenitor cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia
Article Snippet: .. Western blot Immunoblot for Scl -CreERT and Scl:Cdx2 cells was performed using
Article Title: Identification and characterisation of NANOG+/ OCT-4high/SOX2+ doxorubicin-resistant stem-like cells from transformed trophoblastic cell lines
Article Snippet: .. Antibodies For this study, we used anti-OCT4 (1:200 for IF, 1:500 for WB, ab18976 Abcam), anti- SOX2 (1:500 for IF, 1:1000 for WB, ab97959 Abcam), anti-NANOG (1:50 for IF, 1:500 for WB, OAAB11202, Aviva Systems Biology),
Article Title: HpSlyD inducing CDX2 and VIL1 expression mediated through TCTP protein may contribute to intestinal metaplasia in the stomach
Article Snippet: .. The membranes were incubated with primary antibodies overnight at 4 °C:
Article Title: MicroRNA-181d-5p-Containing Exosomes Derived from CAFs Promote EMT by Regulating CDX2/HOXA5 in Breast Cancer
Article Snippet: Next, the sections were blocked with normal goat serum (C-0005; Shanghai Haoran Biological Technology, Shanghai, China) at room temperature for 20 min. Later, the sections were probed with primary antibody rabbit anti-CDX2 (ab88129, 1:100) and HOXA5 (ab82645, 1:100) at 4°C overnight and then with secondary antibody goat anti-rabbit IgG (ab6785, 1:1,000) at 37°C for 20 min.
Article Title: Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression
Article Snippet: .. Subsequently, the membrane was hybridized with primary rabbit anti-human antibodies to