anti perk1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti perk1
    Anti Perk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit perk1 2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit perk1 2
    Rabbit Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti perk1 2 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti perk1 2 antibody
    ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of <t>pERK1/2</t> in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).
    Rabbit Anti Perk1 2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Divergent Pharmacology and Biased Signaling of the Four Melanocortin-4 Receptor Isoforms in Rainbow Trout ( Oncorhynchus mykiss )"

    Article Title: Divergent Pharmacology and Biased Signaling of the Four Melanocortin-4 Receptor Isoforms in Rainbow Trout ( Oncorhynchus mykiss )

    Journal: Biomolecules

    doi: 10.3390/biom13081248

    ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of pERK1/2 in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).
    Figure Legend Snippet: ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of pERK1/2 in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).

    Techniques Used: Western Blot, Expressing, Transfection, Plasmid Preparation, Activity Assay

    rabbit anti perk1 2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti perk1 2
    Rabbit Anti Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti perk1 2  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc rabbit anti perk1 2
    Rabbit Anti Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti perk1 2  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc rabbit anti perk1 2
    Rabbit Anti Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti perk1 2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti perk1 2
    A) Representative western blot for <t>pERK1/2</t> and pTyr-EPHB2 detected in CTRL CRISPR and MYCBP2 CRISPR cells treated with ephrin-B2 (eB2-Fc) for different periods (n=6). Membranes were striped and reblotted with anti-ERK1/2, anti-EPHB2, anti-GAPDH and anti-MYCBP2 antibodies as controls. B) Quantification of EPHB2 tyrosine phosphorylation in CTRL CRISPR cells evoked by ephrin-B2 treatment (15 min, p=0.1363; 30 min, p=0.2056; 1h, p=0.0342; 2h, p=0.0234; 4h, p=0.0068; 8h, p= 0.0231; one-sample t-test). C) Quantification of EPHB2 tyrosine phosphorylation in CTRL CRISPR and MYCBP2 CRISPR HeLa cells (unstimulated, p=0.5589, one-sample t-test; stimulated for 15 min, p=0.7463; 30 min, p=0.5520; 1h, p=0.1920; 2h, p=0.2009; 4h, p=0.1550; 8h, p=0.0331; two-tailed unpaired t-test). D) Quantification of pERK1/2 in CTRL CRISPR and MYCBP2 CRISPR HeLa cells (0 min, p=0.0168, one-sample t-test; 15 min, p=0.6695; 30 min, p= 0.6649; 1h, p= 0.1776; 2h, p= 0.1479; 4h, p= 0.0494; 8h, p= 0.0078; two-tailed unpaired t-test). E) Quantification of EPHB2 in CTRL CRISPR and MYCBP2 CRISPR HeLa (0 min, p=0.4604, one-sample t-test; 15 min, p=0.2222; 30 min, p=0.1376; 1h, p=0.0651; 2h, p=0.0736; 4h, p=0.2451; 8h, p=0.0437, two-tailed unpaired t-test). F) Quantification of ephrin-B2-evoked EPHB2 tyrosine phosphorylation levels relative to total EPHB2 protein levels (0 min, p=0.7058, one-sample t-test; 15 min, p=0.2464; 30 min, p=0.7835; 1h, p=0.9164; 2h, p=0.7196; 4h, p=0.8625; 8h, p=0.5750, two-tailed unpaired t-test). G) Quantification of ephrin-B2-evoked pERK1/2 relative to EPHB2 total protein levels (0 min, p=0.3308, one-sample t-test; 15 min, p=0.3856; 30 min, p=0.0624; 1h, p=0.2683; 2h, p=0.1284; 4h, p=0.7998; 8h, p=0.7790, two-tailed unpaired t-test. Error bars represent SD.
    Rabbit Anti Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function"

    Article Title: Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function

    Journal: bioRxiv

    doi: 10.1101/2023.06.12.544638

    A) Representative western blot for pERK1/2 and pTyr-EPHB2 detected in CTRL CRISPR and MYCBP2 CRISPR cells treated with ephrin-B2 (eB2-Fc) for different periods (n=6). Membranes were striped and reblotted with anti-ERK1/2, anti-EPHB2, anti-GAPDH and anti-MYCBP2 antibodies as controls. B) Quantification of EPHB2 tyrosine phosphorylation in CTRL CRISPR cells evoked by ephrin-B2 treatment (15 min, p=0.1363; 30 min, p=0.2056; 1h, p=0.0342; 2h, p=0.0234; 4h, p=0.0068; 8h, p= 0.0231; one-sample t-test). C) Quantification of EPHB2 tyrosine phosphorylation in CTRL CRISPR and MYCBP2 CRISPR HeLa cells (unstimulated, p=0.5589, one-sample t-test; stimulated for 15 min, p=0.7463; 30 min, p=0.5520; 1h, p=0.1920; 2h, p=0.2009; 4h, p=0.1550; 8h, p=0.0331; two-tailed unpaired t-test). D) Quantification of pERK1/2 in CTRL CRISPR and MYCBP2 CRISPR HeLa cells (0 min, p=0.0168, one-sample t-test; 15 min, p=0.6695; 30 min, p= 0.6649; 1h, p= 0.1776; 2h, p= 0.1479; 4h, p= 0.0494; 8h, p= 0.0078; two-tailed unpaired t-test). E) Quantification of EPHB2 in CTRL CRISPR and MYCBP2 CRISPR HeLa (0 min, p=0.4604, one-sample t-test; 15 min, p=0.2222; 30 min, p=0.1376; 1h, p=0.0651; 2h, p=0.0736; 4h, p=0.2451; 8h, p=0.0437, two-tailed unpaired t-test). F) Quantification of ephrin-B2-evoked EPHB2 tyrosine phosphorylation levels relative to total EPHB2 protein levels (0 min, p=0.7058, one-sample t-test; 15 min, p=0.2464; 30 min, p=0.7835; 1h, p=0.9164; 2h, p=0.7196; 4h, p=0.8625; 8h, p=0.5750, two-tailed unpaired t-test). G) Quantification of ephrin-B2-evoked pERK1/2 relative to EPHB2 total protein levels (0 min, p=0.3308, one-sample t-test; 15 min, p=0.3856; 30 min, p=0.0624; 1h, p=0.2683; 2h, p=0.1284; 4h, p=0.7998; 8h, p=0.7790, two-tailed unpaired t-test. Error bars represent SD.
    Figure Legend Snippet: A) Representative western blot for pERK1/2 and pTyr-EPHB2 detected in CTRL CRISPR and MYCBP2 CRISPR cells treated with ephrin-B2 (eB2-Fc) for different periods (n=6). Membranes were striped and reblotted with anti-ERK1/2, anti-EPHB2, anti-GAPDH and anti-MYCBP2 antibodies as controls. B) Quantification of EPHB2 tyrosine phosphorylation in CTRL CRISPR cells evoked by ephrin-B2 treatment (15 min, p=0.1363; 30 min, p=0.2056; 1h, p=0.0342; 2h, p=0.0234; 4h, p=0.0068; 8h, p= 0.0231; one-sample t-test). C) Quantification of EPHB2 tyrosine phosphorylation in CTRL CRISPR and MYCBP2 CRISPR HeLa cells (unstimulated, p=0.5589, one-sample t-test; stimulated for 15 min, p=0.7463; 30 min, p=0.5520; 1h, p=0.1920; 2h, p=0.2009; 4h, p=0.1550; 8h, p=0.0331; two-tailed unpaired t-test). D) Quantification of pERK1/2 in CTRL CRISPR and MYCBP2 CRISPR HeLa cells (0 min, p=0.0168, one-sample t-test; 15 min, p=0.6695; 30 min, p= 0.6649; 1h, p= 0.1776; 2h, p= 0.1479; 4h, p= 0.0494; 8h, p= 0.0078; two-tailed unpaired t-test). E) Quantification of EPHB2 in CTRL CRISPR and MYCBP2 CRISPR HeLa (0 min, p=0.4604, one-sample t-test; 15 min, p=0.2222; 30 min, p=0.1376; 1h, p=0.0651; 2h, p=0.0736; 4h, p=0.2451; 8h, p=0.0437, two-tailed unpaired t-test). F) Quantification of ephrin-B2-evoked EPHB2 tyrosine phosphorylation levels relative to total EPHB2 protein levels (0 min, p=0.7058, one-sample t-test; 15 min, p=0.2464; 30 min, p=0.7835; 1h, p=0.9164; 2h, p=0.7196; 4h, p=0.8625; 8h, p=0.5750, two-tailed unpaired t-test). G) Quantification of ephrin-B2-evoked pERK1/2 relative to EPHB2 total protein levels (0 min, p=0.3308, one-sample t-test; 15 min, p=0.3856; 30 min, p=0.0624; 1h, p=0.2683; 2h, p=0.1284; 4h, p=0.7998; 8h, p=0.7790, two-tailed unpaired t-test. Error bars represent SD.

    Techniques Used: Western Blot, CRISPR, Two Tailed Test

    perk1 2 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc perk1 2 rabbit mab
    Perk1 2 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit α perk1 2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit α perk1 2
    Rabbit α Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti perk1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc anti perk1
    Anti Perk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti perk1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc anti perk1

    Anti Perk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti perk1/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    anti perk1 - by Bioz Stars, 2023-09
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    1) Product Images from "Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities"

    Article Title: Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities

    Journal: eLife

    doi: 10.7554/eLife.78629


    Figure Legend Snippet:

    Techniques Used: Knock-Out, Transgenic Assay, Recombinant, Plasmid Preparation, Clone Assay, Sequencing, DC Protein Assay, Transformation Assay, Isolation, Protease Inhibitor, Western Blot, Software

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    • anti perk1  (Cell Signaling Technology Inc)
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    rabbit perk1 2  (Cell Signaling Technology Inc)
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    ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of <t>pERK1/2</t> in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).
    Rabbit Anti Perk1 2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti perk1 2  (Cell Signaling Technology Inc)
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    ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of <t>pERK1/2</t> in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).
    Rabbit Anti Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    perk1 2 rabbit mab  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc perk1 2 rabbit mab
    ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of <t>pERK1/2</t> in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).
    Perk1 2 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/perk1 2 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    perk1 2 rabbit mab - by Bioz Stars, 2023-09
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    rabbit α perk1 2  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc rabbit α perk1 2
    ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of <t>pERK1/2</t> in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).
    Rabbit α Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit α perk1 2/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit α perk1 2 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier

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    ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of pERK1/2 in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).

    Journal: Biomolecules

    Article Title: Divergent Pharmacology and Biased Signaling of the Four Melanocortin-4 Receptor Isoforms in Rainbow Trout ( Oncorhynchus mykiss )

    doi: 10.3390/biom13081248

    Figure Lengend Snippet: ERK1/2 signaling properties of trout Mc4rs. ( A ) Immunoblots of pERK1/2 in cells expressing omMc4ra1, omMc4ra2, omMc4rb1, or omMc4rb2. HEK293T cells were transiently transfected with omMc4ra1, omMc4ra2, omMc4rb1, omMc4rb2, or empty vector plasmids and were starved overnight 24 h after transfection. Cells were treated with vehicle only or with 10 nM AgRP, 1 μM Ipsen 5i, 1 μM ML00253764, or 1 μM MCL0020 for 5 min. Western blots were performed as described in Materials and Methods. ( B ) Densitometry results of basal pERK1/2 levels of omMc4ra1, omMc4ra2, omMc4rb1, and omMc4rb2. ( C ) Densitometry results of pERK1/2 level of omMc4ra1. ( D ) Densitometry results of pERK1/2 level of omMc4ra2. ( E ) Densitometry results of pERK1/2 level of omMc4rb1. ( F ) Densitometry results of pERK1/2 level of omMc4rb2. Data are mean ± SEM from at least three independent experiments. * Significantly different from basal or vector activity (* p < 0.05, and ** p < 0.01).

    Article Snippet: Rabbit anti-pERK1/2 antibody (Catalog #4370, Cell Signaling, Beverly, MA, USA) and mouse anti-β-tubulin antibody (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA) were used in this study.

    Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Activity Assay