Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Source, application and concentration of antibodies.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Concentration Assay, Recombinant, Plasmid Preparation

Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Mass Spectrometry

Journal: Jornal Brasileiro de Pneumologia

Article Title: Diagnostic value of α-enolase expression and serum α-enolase autoantibody levels in lung cancer

doi: 10.1590/S1806-37562016000000241

Figure Lengend Snippet: Photomicrographs showing α-enolase (ENO1) expression in lung tissue samples (immunohistochemistry using the streptavidin-peroxidase staining method). Positive signs are visualized as yellow or brownish yellow granules. In A and B, adenocarcinoma tissue samples showing positive ENO1 expression (magnification, ×400 and ×100, respectively). In C and D, squamous cell carcinoma tissue samples showing positive ENO1 expression (magnification, ×400 and ×100, respectively). In E and F, pulmonary inflammatory pseudotumor tissue samples showing negative ENO1 expression (magnification, ×400 and ×100, respectively).

Article Snippet: The reagents used were rabbit anti-ENO1 monoclonal antibody (Abcam Biotechnology Co. Ltd., Cambridge, UK), an immunohistochemistry reagent kit (Maixin, Fuzhou, China) and an ENO1 antibody ELISA reagent kit (HuaAn, Hangzhou, China).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Jornal Brasileiro de Pneumologia

Article Title: Diagnostic value of α-enolase expression and serum α-enolase autoantibody levels in lung cancer

doi: 10.1590/S1806-37562016000000241

Figure Lengend Snippet: ROC curve showing the sensitivity and specificity of serum α-enolase antibody levels for the diagnosis of lung cancer.

Article Snippet: The reagents used were rabbit anti-ENO1 monoclonal antibody (Abcam Biotechnology Co. Ltd., Cambridge, UK), an immunohistochemistry reagent kit (Maixin, Fuzhou, China) and an ENO1 antibody ELISA reagent kit (HuaAn, Hangzhou, China).

Techniques:

Journal: iScience

Article Title: Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling

doi: 10.1016/j.isci.2021.103607

Figure Lengend Snippet: Proteo-transcriptomic integration confirms HIF signaling and glycolysis as unique features of the male EC phenotype Proteo-transcriptomic analysis in HC (n = 9 ), EC (n = 9 ), and VP (n = 9 ). (A) Upset plot describing the number of features detected by each of the sparse partial least squares models. (B) Schematic representation of the derivation of EC-specific proteins. (C) PCA plot representing sample clustering with respect to EC-specific proteins. The first two principal components capturing maximum of variance were used. Data ellipses were drawn at the level of 0.9. (D) Heatmap visualizing quantile normalized and Z-scaled expression pattern of EC-specific proteins. Column annotation represents the cohorts and the different groups used for pairwise comparison. Proteins are hierarchically clustered based on Euclidean distance. (E) Functional analysis results of proteins belonging to cluster 1 in (D). Size of the bubble and the color gradient are relative to number of features in each pathway and adjusted p value of the enrichment test, respectively. (F) Functional analysis results of the proteins belonging to cluster 2 in (D). The bubble size and color gradient are relative to number of features in each pathway and adjusted p value of the enrichment test, respectively. (G) Schematic representation of the proteins belonging to pathways in (F). Circular nodes in red represents upregulated proteins in EC, in relation to HC, and gray color denotes non-significant expression levels. (H) Western blot analysis of HIF target genes identified in (G) for male HC (n = 9 ), and EC (n = 9 ). (I) Violin plots representing relative protein quantification of HIF-1α, ENO1, and ENO3 normalized to β-Actin from (H) using unpaired t test (significance level, p < 0.05) represented with violin plot with mean. See also Figure S2 .

Article Snippet: Rabbit monoclonal anti-ENO1 [EPR10863(B)] , Abcam , Cat#ab155102.

Techniques: Expressing, Functional Assay, Western Blot

Journal: iScience

Article Title: Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling

doi: 10.1016/j.isci.2021.103607

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-ENO1 [EPR10863(B)] , Abcam , Cat#ab155102.

Techniques: Software

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: ENO1 expression in liver cancer tissue and peripheral blood of patients with liver cancer. (A) ENO1 expression in liver cancer tissues (×100). (B) ENO1 was not expressed in benign liver lesions (×100). (C) Serum anti-ENO1 antibody levels in patients with liver cancer were higher than those in patients with benign liver lesions and healthy controls. (D) ENO1 expression in liver cancer tissues (×400). (E) ENO1 was not expressed in benign liver lesion (×400). (F) Receiver operating characteristic curve analysis for liver cancer diagnosis using anti-ENO1 antibody levels. The AUC was 0.741, the sensitivity was 64.3% and the specificity was 85.5%. The red dot represents the cut-off value. **P<0.01; ****P<0.0001 ENO1, α-enolase; AUC, area under the curve.

Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

Techniques: Expressing

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: ENO1 expression in pathological tissues.

Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

Techniques: Expressing

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Comparison of the serum anti-ENO1 antibody levels among the three groups of participants [P50 (P25-P75)].

Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

Techniques:

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Validation of the siRNA interference effect on ENO1 expression in liver cancer cells. (A) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (B) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (C) Western blot validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 protein in HepG2 cells in the si-1 and si-2 groups were significantly lower than those in the control group. (D) Western blot validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 protein in Huh7 cells in the si-1 and si-2 groups were significantly lower than those in the control group. **P<0.01; ****P<0.0001. siRNA, small interfering RNA; ENO1, α-enolase; si-1, siRNA interference group 1; si-2, siRNA interference group 2; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Small Interfering RNA, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Effect of ENO1 siRNA on liver cancer cell proliferation. (A) HepG2 and (B) Huh7 cell proliferation was suppressed after ENO1 siRNA treatment. Results show that 72 h after transfection in the si-1 and si-2 groups, ENO1 siRNA treatment resulted in proliferation inhibition in HepG2 and Huh7 cells. In HepG2 cells, the differences in cell proliferation between the NC and the siRNA-treated groups were statistically significant (P<0.05 vs. si-2 group and P<0.01 vs. si-1 group). Cell proliferation in Huh7 cells was also significantly different. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; si-1, siRNA interference group 1; si-2, siRNA interference group 2; OD, optical density; NC, negative control.

Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

Techniques: Transfection, Inhibition, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Effect of ENO1 siRNA on the migration ability of liver cancer cells. (A) Compared with that of the NC group, HepG2 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (B) Gap size was measured using ImageJ software and the ratio of gap size after ENO1 siRNA treatment in HepG2 cells was calculated based on the size of the wound at 0 h. (C) Compared with that of the NC group, Huh7 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (D) Gap size was measured with the ImageJ software and the ratio of gap size after ENO1 siRNA treatment in Huh7 cell was calculated based on the size of the wound at 0 h. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

Techniques: Migration, Software, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Effect of ENO1 siRNA on the invasion and migration abilities of liver cancer cells. Compared with the NC group, the in vitro invasion and migration abilities of (A) HepG2 and (B) Huh7 cells after ENO1 siRNA treatment decreased in the si-1 and si-2 groups, and the differences were statistically significant. Numbers of (C) HepG2 and (D) Huh7 cells were measured with the ImageJ software after ENO1 siRNA treatment. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

Techniques: Migration, In Vitro, Software, Small Interfering RNA, Negative Control

Journal: Molecular cell

Article Title: p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis

doi: 10.1016/j.molcel.2018.04.011

Figure Lengend Snippet: (A) Both PFK and ENO1 enzymes display reduced activities in p300 KO HCT116 cells. WT and p300 KO HCT116 cells were cultured in complete medium. The activities of PFK and ENO1 from total cell lysates were measured using colorimetric assay kits (n=3, *p<0.05, values are expressed as mean ±SEM).

Article Snippet: Rabbit monoclonal anti-ENO1 antibody (clone {"type":"entrez-protein","attrs":{"text":"EPR10864","term_id":"523376409","term_text":"EPR10864"}} EPR10864 (B)) , Abcam , Cat# ab155955.

Techniques: Cell Culture, Colorimetric Assay

Journal: Molecular cell

Article Title: p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis

doi: 10.1016/j.molcel.2018.04.011

Figure Lengend Snippet: Key Resources Table

Article Snippet: Rabbit monoclonal anti-ENO1 antibody (clone {"type":"entrez-protein","attrs":{"text":"EPR10864","term_id":"523376409","term_text":"EPR10864"}} EPR10864 (B)) , Abcam , Cat# ab155955.

Techniques: Recombinant, shRNA, Clone Assay, Synthesized, Activity Assay, Mutagenesis, Mass Spectrometry, Western Blot, Sequencing, Software