rabbit monoclonal antibody  (Sino Biological)


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    Name:
    MERS CoV Spike Antibody Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with purified recombinant recombinant MERS CoV NCoV Novel coronavirus Spike Protein Catalog 40069 V08B AFS88936 1 Met1 Trp1297
    Catalog Number:
    40069-R723
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    MERS CoV
    Applications:
    Microneutralizaiton(MN)
    Immunogen:
    Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297) (Catalog#40069-V08B)
    Product Aliases:
    Anti-coronavirus s1 Antibody, Anti-coronavirus s2 Antibody, Anti-coronavirus spike Antibody, Anti-cov spike Antibody, Anti-ncov RBD Antibody, Anti-ncov s1 Antibody, Anti-ncov s2 Antibody, Anti-ncov spike Antibody, Anti-RBD Antibody, Anti-S Antibody, Anti-s1 Antibody, Anti-Spike RBD Antibody
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological rabbit monoclonal antibody
    This antibody was obtained from a rabbit immunized with purified recombinant recombinant MERS CoV NCoV Novel coronavirus Spike Protein Catalog 40069 V08B AFS88936 1 Met1 Trp1297
    https://www.bioz.com/result/rabbit monoclonal antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibody - by Bioz Stars, 2021-06
    94/100 stars

    Images

    Related Articles

    Positive Control:

    Article Title: Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial
    Article Snippet: Two-fold diluted serum was incubated with 2 × 106 viral particles of pseudotyped virus for 60 min at 37°C and added to 786-O cells, incubated for 6 h, and replaced with fresh medium; luciferase-reporter activity was measured 3 days later. .. A commercially available neutralising monoclonal antibody (40069-R723, Sino Biological, Beijing, China) was used as a positive control. .. To assess cellular immunity, interferon-γ-linked enzyme-linked immunospot (ELISpot) assays were done with fresh peripheral blood mononuclear cells (PBMCs) to determine responses to the MERS-CoV spike vaccine antigen.

    Infection:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Article Title: Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.
    Article Snippet: Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat.

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Immunofluorescence:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Staining:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Article Title: Diffuse High Intensity PD-L1 Staining in Thymic Epithelial Tumors
    Article Snippet: .. The TMA was stained with rabbit monoclonal antibody (clone 15, Sino Biological) to human PD-L1. .. PD-L1 staining was scored based on intensity as follows: 0=none, 1=equivocal/uninterpretable, 2=weak, and 3=intermediate-strong.

    Incubation:

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning
    Article Snippet: .. The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT. ..

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Article Title: Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.
    Article Snippet: Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat.

    Blocking Assay:

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Luciferase:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Activity Assay:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Derivative Assay:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

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  • 93
    Sino Biological ccl2 neutralizing antibody
    Erdr1 enhances the proliferation and migration of HDFs by inducing the production of <t>CCL2</t> via the activation of MAPK kinases. (A) The levels of ERK1/2, p-ERK1/2, p38, p-p38, JNK1/2 and p-JNK1/2 in HDFs treated with 50 ng/ml Erdr1, EGF or PBs for 5, 15, 30, 45 and 60 min were analyzed by western blotting. Tubulin was used as the loading control. The (B) proliferation and (C) migration of HDFs treated with 50 ng/ml Erdr1 or DMSO (control) for 24 h after pretreatment with the ERK1/2 inhibitor u0126 (20 µ M), the p38 inhibitor sB203580 (20 µ M) or the JNK1/2 inhibitor sP600125 (20 µ M) for 1 h was determined using the MTT and wound healing assays, respectively. n=6. (D) The levels of CCL2 protein were determined in HDFs pre-treated with 20 µ M u0126, sB203580 or sP600125 for 1 h and subsequently treated with 50 ng/ml Erdr1 for 24 h using ELISA. n=6. * P
    Ccl2 Neutralizing Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ccl2 neutralizing antibody/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ccl2 neutralizing antibody - by Bioz Stars, 2021-06
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    88
    Sino Biological cadm3
    eSIBR enhances multi-target knockdown potency in primary neuron cultures. ( A ) Schematic of lentiviral vectors used for multiple endogenous gene knockdown. The cytomegalovirus promoter (CMV, yellow) promotes antisense-strand expression (relative to viral RNA) of amiRNAs located in an intron preceding a nuclear-localized GFP (nlsGFP, green) coding sequence. amiRNA triplets target cadm1, 2 and 3 or contain three unique scrambled sequences (scrambled1, 2 and 3). Orange boxes represent viral-specific sequences. LTR, long-terminal repeat, RRE, Rev-response element, cPPT, central polypurine tract, WPRE, woodchuck hepatitis virus posttranscriptional regulatory element, SA, splice acceptor, SD, splice donor. ( B ) Representative western blots and ( C ) quantification of endogenous Cadm family knockdown efficiency in 14DIV cultured rat hippocampal neurons transduced with viral constructs carrying the indicated amiRNA sequences in wtSIBR or eSIBR backbones. Antibodies were against Cadm1, <t>Cadm3</t> or Cadm1–3 (pleio-Cadm). Knockdown percentage was calculated relative to uninfected control cultures (no virus). Actin was used as a loading control. ( D ) Comparison of endogenous Cadm potency-of-knockdown for viral vectors containing amiRNAs in eSIBR backbones (blue bars) relative to their counterparts in the wtSIBR backbone (black bars). (C) and (D) n = 4 independent experiments, * P
    Cadm3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cadm3/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cadm3 - by Bioz Stars, 2021-06
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    94
    Sino Biological influenza a virus hemagglutinin ha antibody rabbit mab
    Effect of λ-CGN on <t>influenza</t> A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice
    Influenza A Virus Hemagglutinin Ha Antibody Rabbit Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a virus hemagglutinin ha antibody rabbit mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a virus hemagglutinin ha antibody rabbit mab - by Bioz Stars, 2021-06
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    92
    Sino Biological unconjugated rabbit rsv f monoclonal antibody
    Non-replicating mouse codon-optimized recombinant adenovirus vaccines construction and in vitro protein expression. ( A ) Schematic representation of the Ad constructs. Both Ad-SF and Ad-SF40L express the full length <t>RSV</t> F protein preceded by a secretion signal, S. Ad-SF40L also encodes the full length mouse CD40 ligand, CD40L, following a trimerization motif, T. Ad-Empty does not encode for RSV F or CD40L whereas Ad-SNP40L encodes the influenza A nucleoprotein (NP) followed by CD40L as previously described. ( B ) Representative images at 10x magnification of in vitro protein expression following an immunofluorescence assay with a rabbit RSV F antibody along with Alexa Fluor 594-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse CD40L. A merge of the two fluorochromes show the co-expression of RSV F and CD40L. Data are representative of two experiments.
    Unconjugated Rabbit Rsv F Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated rabbit rsv f monoclonal antibody/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated rabbit rsv f monoclonal antibody - by Bioz Stars, 2021-06
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    Erdr1 enhances the proliferation and migration of HDFs by inducing the production of CCL2 via the activation of MAPK kinases. (A) The levels of ERK1/2, p-ERK1/2, p38, p-p38, JNK1/2 and p-JNK1/2 in HDFs treated with 50 ng/ml Erdr1, EGF or PBs for 5, 15, 30, 45 and 60 min were analyzed by western blotting. Tubulin was used as the loading control. The (B) proliferation and (C) migration of HDFs treated with 50 ng/ml Erdr1 or DMSO (control) for 24 h after pretreatment with the ERK1/2 inhibitor u0126 (20 µ M), the p38 inhibitor sB203580 (20 µ M) or the JNK1/2 inhibitor sP600125 (20 µ M) for 1 h was determined using the MTT and wound healing assays, respectively. n=6. (D) The levels of CCL2 protein were determined in HDFs pre-treated with 20 µ M u0126, sB203580 or sP600125 for 1 h and subsequently treated with 50 ng/ml Erdr1 for 24 h using ELISA. n=6. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Erythroid differentiation regulator 1 promotes wound healing by inducing the production of C-C motif chemokine ligand 2 via the activation of MAP kinases in vitro and in vivo

    doi: 10.3892/ijmm.2020.4762

    Figure Lengend Snippet: Erdr1 enhances the proliferation and migration of HDFs by inducing the production of CCL2 via the activation of MAPK kinases. (A) The levels of ERK1/2, p-ERK1/2, p38, p-p38, JNK1/2 and p-JNK1/2 in HDFs treated with 50 ng/ml Erdr1, EGF or PBs for 5, 15, 30, 45 and 60 min were analyzed by western blotting. Tubulin was used as the loading control. The (B) proliferation and (C) migration of HDFs treated with 50 ng/ml Erdr1 or DMSO (control) for 24 h after pretreatment with the ERK1/2 inhibitor u0126 (20 µ M), the p38 inhibitor sB203580 (20 µ M) or the JNK1/2 inhibitor sP600125 (20 µ M) for 1 h was determined using the MTT and wound healing assays, respectively. n=6. (D) The levels of CCL2 protein were determined in HDFs pre-treated with 20 µ M u0126, sB203580 or sP600125 for 1 h and subsequently treated with 50 ng/ml Erdr1 for 24 h using ELISA. n=6. * P

    Article Snippet: To evaluate the effects of the CCL2-neutralizing antibody (Sino Biological, Inc.) on cell proliferation, HDFs were treated with 50 ng/ml Erdr1 or PBs for 24 h following pretreatment with 1, 10 or 30 µ g/ml CCL2-neutralizing antibody.

    Techniques: Migration, Activation Assay, Western Blot, MTT Assay, Enzyme-linked Immunosorbent Assay

    Erdr1 induces the production of CCL2 in HDFs. (A and B) The mRNA expression levels of CCL2 mRNA in HDFs treated with Erdr1, EGF or PBs (A) at various concentrations and (B) at different time points were determined using reverse transcription-PCR. (C) The protein levels of CCL2 in HDFs treated with Erdr1, EGF or PBs for 24 h were determined using ELISA. n=6. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Erythroid differentiation regulator 1 promotes wound healing by inducing the production of C-C motif chemokine ligand 2 via the activation of MAP kinases in vitro and in vivo

    doi: 10.3892/ijmm.2020.4762

    Figure Lengend Snippet: Erdr1 induces the production of CCL2 in HDFs. (A and B) The mRNA expression levels of CCL2 mRNA in HDFs treated with Erdr1, EGF or PBs (A) at various concentrations and (B) at different time points were determined using reverse transcription-PCR. (C) The protein levels of CCL2 in HDFs treated with Erdr1, EGF or PBs for 24 h were determined using ELISA. n=6. * P

    Article Snippet: To evaluate the effects of the CCL2-neutralizing antibody (Sino Biological, Inc.) on cell proliferation, HDFs were treated with 50 ng/ml Erdr1 or PBs for 24 h following pretreatment with 1, 10 or 30 µ g/ml CCL2-neutralizing antibody.

    Techniques: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    CCL2-neutralizing antibody inhibits the proliferation and migration of HDFs. (A) The proliferation of HDFs treated with 50 ng/ml Erdr1 or PBs at the indicated concentrations for 24 h following pretreatment with 1, 10 or 30 µ g/ml CCL2-neutralizing antibody was determined using the MTT assay. n=6. (B) Wound healing assay in HDFs treated with 50 ng/ml Erdr1 or PBs for 24 h following pretreatment with 1, 10 or 30 µ g/ml CCL2-neutralizing antibody. n=6. (C) The proposed scheme of the therapeutic action of Erdr1 in wound repair. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Erythroid differentiation regulator 1 promotes wound healing by inducing the production of C-C motif chemokine ligand 2 via the activation of MAP kinases in vitro and in vivo

    doi: 10.3892/ijmm.2020.4762

    Figure Lengend Snippet: CCL2-neutralizing antibody inhibits the proliferation and migration of HDFs. (A) The proliferation of HDFs treated with 50 ng/ml Erdr1 or PBs at the indicated concentrations for 24 h following pretreatment with 1, 10 or 30 µ g/ml CCL2-neutralizing antibody was determined using the MTT assay. n=6. (B) Wound healing assay in HDFs treated with 50 ng/ml Erdr1 or PBs for 24 h following pretreatment with 1, 10 or 30 µ g/ml CCL2-neutralizing antibody. n=6. (C) The proposed scheme of the therapeutic action of Erdr1 in wound repair. * P

    Article Snippet: To evaluate the effects of the CCL2-neutralizing antibody (Sino Biological, Inc.) on cell proliferation, HDFs were treated with 50 ng/ml Erdr1 or PBs for 24 h following pretreatment with 1, 10 or 30 µ g/ml CCL2-neutralizing antibody.

    Techniques: Migration, MTT Assay, Wound Healing Assay

    eSIBR enhances multi-target knockdown potency in primary neuron cultures. ( A ) Schematic of lentiviral vectors used for multiple endogenous gene knockdown. The cytomegalovirus promoter (CMV, yellow) promotes antisense-strand expression (relative to viral RNA) of amiRNAs located in an intron preceding a nuclear-localized GFP (nlsGFP, green) coding sequence. amiRNA triplets target cadm1, 2 and 3 or contain three unique scrambled sequences (scrambled1, 2 and 3). Orange boxes represent viral-specific sequences. LTR, long-terminal repeat, RRE, Rev-response element, cPPT, central polypurine tract, WPRE, woodchuck hepatitis virus posttranscriptional regulatory element, SA, splice acceptor, SD, splice donor. ( B ) Representative western blots and ( C ) quantification of endogenous Cadm family knockdown efficiency in 14DIV cultured rat hippocampal neurons transduced with viral constructs carrying the indicated amiRNA sequences in wtSIBR or eSIBR backbones. Antibodies were against Cadm1, Cadm3 or Cadm1–3 (pleio-Cadm). Knockdown percentage was calculated relative to uninfected control cultures (no virus). Actin was used as a loading control. ( D ) Comparison of endogenous Cadm potency-of-knockdown for viral vectors containing amiRNAs in eSIBR backbones (blue bars) relative to their counterparts in the wtSIBR backbone (black bars). (C) and (D) n = 4 independent experiments, * P

    Journal: Nucleic Acids Research

    Article Title: Improved knockdown from artificial microRNAs in an enhanced miR-155 backbone: a designer's guide to potent multi-target RNAi

    doi: 10.1093/nar/gkv1246

    Figure Lengend Snippet: eSIBR enhances multi-target knockdown potency in primary neuron cultures. ( A ) Schematic of lentiviral vectors used for multiple endogenous gene knockdown. The cytomegalovirus promoter (CMV, yellow) promotes antisense-strand expression (relative to viral RNA) of amiRNAs located in an intron preceding a nuclear-localized GFP (nlsGFP, green) coding sequence. amiRNA triplets target cadm1, 2 and 3 or contain three unique scrambled sequences (scrambled1, 2 and 3). Orange boxes represent viral-specific sequences. LTR, long-terminal repeat, RRE, Rev-response element, cPPT, central polypurine tract, WPRE, woodchuck hepatitis virus posttranscriptional regulatory element, SA, splice acceptor, SD, splice donor. ( B ) Representative western blots and ( C ) quantification of endogenous Cadm family knockdown efficiency in 14DIV cultured rat hippocampal neurons transduced with viral constructs carrying the indicated amiRNA sequences in wtSIBR or eSIBR backbones. Antibodies were against Cadm1, Cadm3 or Cadm1–3 (pleio-Cadm). Knockdown percentage was calculated relative to uninfected control cultures (no virus). Actin was used as a loading control. ( D ) Comparison of endogenous Cadm potency-of-knockdown for viral vectors containing amiRNAs in eSIBR backbones (blue bars) relative to their counterparts in the wtSIBR backbone (black bars). (C) and (D) n = 4 independent experiments, * P

    Article Snippet: We measured protein levels with antibodies against endogenous Cadm1, Cadm3 or pleio-Cadm, which recognizes Cadms 1, 2 and 3 , and measured knockdown efficiency by normalizing values to non-infected sister cultures (no virus) (Figure ).

    Techniques: Expressing, Sequencing, Western Blot, Cell Culture, Transduction, Construct

    Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, In Vivo, Mouse Assay

    Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, Concentration Assay

    Non-replicating mouse codon-optimized recombinant adenovirus vaccines construction and in vitro protein expression. ( A ) Schematic representation of the Ad constructs. Both Ad-SF and Ad-SF40L express the full length RSV F protein preceded by a secretion signal, S. Ad-SF40L also encodes the full length mouse CD40 ligand, CD40L, following a trimerization motif, T. Ad-Empty does not encode for RSV F or CD40L whereas Ad-SNP40L encodes the influenza A nucleoprotein (NP) followed by CD40L as previously described. ( B ) Representative images at 10x magnification of in vitro protein expression following an immunofluorescence assay with a rabbit RSV F antibody along with Alexa Fluor 594-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse CD40L. A merge of the two fluorochromes show the co-expression of RSV F and CD40L. Data are representative of two experiments.

    Journal: Scientific Reports

    Article Title: Targeting CD40 enhances antibody- and CD8-mediated protection against respiratory syncytial virus infection

    doi: 10.1038/s41598-018-34999-z

    Figure Lengend Snippet: Non-replicating mouse codon-optimized recombinant adenovirus vaccines construction and in vitro protein expression. ( A ) Schematic representation of the Ad constructs. Both Ad-SF and Ad-SF40L express the full length RSV F protein preceded by a secretion signal, S. Ad-SF40L also encodes the full length mouse CD40 ligand, CD40L, following a trimerization motif, T. Ad-Empty does not encode for RSV F or CD40L whereas Ad-SNP40L encodes the influenza A nucleoprotein (NP) followed by CD40L as previously described. ( B ) Representative images at 10x magnification of in vitro protein expression following an immunofluorescence assay with a rabbit RSV F antibody along with Alexa Fluor 594-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse CD40L. A merge of the two fluorochromes show the co-expression of RSV F and CD40L. Data are representative of two experiments.

    Article Snippet: After blocking with 3% IgG-free BSA diluted in wash buffer (1x PBS with 0.1% Tween 20) for 1 hour at 37 °C, the cells were stained with an unconjugated Rabbit RSV F monoclonal antibody (Sino Biological; clone #009) for 1 hour at 37 °C.

    Techniques: Recombinant, In Vitro, Expressing, Construct, Immunofluorescence