rabbit monoclonal antibodies against s1pr1  (Abcam)

 
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    Abcam rabbit monoclonal antibodies against s1pr1
    Correlation of <t>S1PR1/STAT3</t> signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.
    Rabbit Monoclonal Antibodies Against S1pr1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibodies against s1pr1/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibodies against s1pr1 - by Bioz Stars, 2021-06
    99/100 stars

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    1) Product Images from "S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation"

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    Journal: EBioMedicine

    doi: 10.1016/j.ebiom.2018.10.005

    Correlation of S1PR1/STAT3 signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.
    Figure Legend Snippet: Correlation of S1PR1/STAT3 signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.

    Techniques Used:

    Gastric cancer cell lines have different sensitivity to DDP treatment. (A) Cell viability of SGC7901 and SGC7901/DDP cells, cells were treated with fold diluted DDP. Data were expressed as mean ± SD, n = 4. (B) Cell viability of indicated gastric tumor cells, cells were treated with fold diluted DDP after transfection of S1PR1 overexpression or empty vector. Data were expressed as mean ± SD, n = 4. (C) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a progressive disease outcome. (D) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a complete remission/response outcome.
    Figure Legend Snippet: Gastric cancer cell lines have different sensitivity to DDP treatment. (A) Cell viability of SGC7901 and SGC7901/DDP cells, cells were treated with fold diluted DDP. Data were expressed as mean ± SD, n = 4. (B) Cell viability of indicated gastric tumor cells, cells were treated with fold diluted DDP after transfection of S1PR1 overexpression or empty vector. Data were expressed as mean ± SD, n = 4. (C) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a progressive disease outcome. (D) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a complete remission/response outcome.

    Techniques Used: Transfection, Over Expression, Plasmid Preparation

    S1PR1 and pY-STAT3 were highly expressed in gastric cancer patients. (A) upper panel, Kaplan-Meier plots for overall survival of high and low transcript levels of S1PR1 in gastric cancer patients; lower panel transcript levels of S1PR1 in 4 stages of gastric cancer patients. (B) Immunohistochemistry staining of S1PR1 of gastric cancer patient tissues. Upper panel: a stage IIIb sample, lower panel: a stage IIa sample. Scale bar: 200 μM left panel, 100 μM right panel. (C) Immuno score levels of S1PR1 in different stage of gastric cancer patients were compared. (D) Correlation of S1PR1 and STAT3 transcript levels in gastric cancer patients. (E) Immunohistochemistry staining of pY-STAT3 of gastric cancer patient tissues. Upper panel stage IIIc, lower panel stage IIa Scale bar: 200 μM left panel, 100 μM right panel. (F) Correlation of S1PR1 and pY-STAT3 protein levels determined by IS in gastric cancer patients. (G) Left panel: Immunofluorescence double staining of S1PR1(green) and pY-STAT3(red) in gastric tumor, para-cancerous and normal samples, DAPI(blue) was adopted to reveal the nuclear of the cells. Scale bar: 20 μM. Right panel, quantification of patient tissue sections for percentages of overlapping red (pY-STAT3) and green (S1PR1) channels, shown as Manders colocalization coefficients M1 (p-STAT3 to S1PR1) and M2 (S1PR1 to p-STAT3), respectively.
    Figure Legend Snippet: S1PR1 and pY-STAT3 were highly expressed in gastric cancer patients. (A) upper panel, Kaplan-Meier plots for overall survival of high and low transcript levels of S1PR1 in gastric cancer patients; lower panel transcript levels of S1PR1 in 4 stages of gastric cancer patients. (B) Immunohistochemistry staining of S1PR1 of gastric cancer patient tissues. Upper panel: a stage IIIb sample, lower panel: a stage IIa sample. Scale bar: 200 μM left panel, 100 μM right panel. (C) Immuno score levels of S1PR1 in different stage of gastric cancer patients were compared. (D) Correlation of S1PR1 and STAT3 transcript levels in gastric cancer patients. (E) Immunohistochemistry staining of pY-STAT3 of gastric cancer patient tissues. Upper panel stage IIIc, lower panel stage IIa Scale bar: 200 μM left panel, 100 μM right panel. (F) Correlation of S1PR1 and pY-STAT3 protein levels determined by IS in gastric cancer patients. (G) Left panel: Immunofluorescence double staining of S1PR1(green) and pY-STAT3(red) in gastric tumor, para-cancerous and normal samples, DAPI(blue) was adopted to reveal the nuclear of the cells. Scale bar: 20 μM. Right panel, quantification of patient tissue sections for percentages of overlapping red (pY-STAT3) and green (S1PR1) channels, shown as Manders colocalization coefficients M1 (p-STAT3 to S1PR1) and M2 (S1PR1 to p-STAT3), respectively.

    Techniques Used: Immunohistochemistry, Staining, Immunofluorescence, Double Staining

    S1PR1-STAT3 formed a positive regulatory loop in GC cells and contributed to drug resistance. (A) Expression of indicated genes of SGC7901/DDP cells after treated with different concentrations of S1P and DDP for 24 h was detected by Western blot. (B) Expression of indicated genes of SGC7901 cells transfected with S1PR1 over-expression and empty vectors for 48 h was detected by Western blot. (C) SGC7901 cells transfected with S1PR1 overexpression or empty vector cells were treated with fold diluted DDP for 24 h and the cell viability was detected by MTS method. Data were expressed as mean ± SD, n = 4. (D) Expression of indicated genes of SGC7901/DDP cells transfected with S1PR1 siRNA and mock siRNA for 48 h was determined by Western blot. (E) Cell viability of SGC7901/DDP cells, tested by MTS method. Cells were treated with fold diluted DDP for 24 h with or without S1PR1 knocking down. Data were expressed as mean ± SD, n = 4. (F) Cell apoptosis levels of SGC7091/DDP cells were determined by AnnexinV/PI double staining with FACS after indicated treatment for 24 h. The ratio of double negative population of the cells was presented with mean ± SD, n = 5. *P
    Figure Legend Snippet: S1PR1-STAT3 formed a positive regulatory loop in GC cells and contributed to drug resistance. (A) Expression of indicated genes of SGC7901/DDP cells after treated with different concentrations of S1P and DDP for 24 h was detected by Western blot. (B) Expression of indicated genes of SGC7901 cells transfected with S1PR1 over-expression and empty vectors for 48 h was detected by Western blot. (C) SGC7901 cells transfected with S1PR1 overexpression or empty vector cells were treated with fold diluted DDP for 24 h and the cell viability was detected by MTS method. Data were expressed as mean ± SD, n = 4. (D) Expression of indicated genes of SGC7901/DDP cells transfected with S1PR1 siRNA and mock siRNA for 48 h was determined by Western blot. (E) Cell viability of SGC7901/DDP cells, tested by MTS method. Cells were treated with fold diluted DDP for 24 h with or without S1PR1 knocking down. Data were expressed as mean ± SD, n = 4. (F) Cell apoptosis levels of SGC7091/DDP cells were determined by AnnexinV/PI double staining with FACS after indicated treatment for 24 h. The ratio of double negative population of the cells was presented with mean ± SD, n = 5. *P

    Techniques Used: Expressing, Western Blot, Transfection, Over Expression, Plasmid Preparation, Double Staining, FACS

    Hyper expression of S1PR1 in gastric cancer cells identified drug resistance. (A) Transcript levels determined by QPCR and (B) protein expression levels determined by Western blot of indicated genes of SGC7901 and SGC7901/DDP gastric cancer cell lines. Data were expressed as mean ± SD, n = 5. *P
    Figure Legend Snippet: Hyper expression of S1PR1 in gastric cancer cells identified drug resistance. (A) Transcript levels determined by QPCR and (B) protein expression levels determined by Western blot of indicated genes of SGC7901 and SGC7901/DDP gastric cancer cell lines. Data were expressed as mean ± SD, n = 5. *P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    FTY720 enhanced gastric tumor cells drug sensitivity in vivo . (A) Images of tumor bearing mice of each treatment group at the end of the experiment. (B) Body weight of mice of tumor bearing mice of each treatment group. Data were expressed as mean ± SD, n = 10. (C) H E staining and Immunohistochemistry staining of S1PR1, pY-STAT3 and Ki67 of gastric tumor tissue removed from mice. Scale bar: 10 μM.
    Figure Legend Snippet: FTY720 enhanced gastric tumor cells drug sensitivity in vivo . (A) Images of tumor bearing mice of each treatment group at the end of the experiment. (B) Body weight of mice of tumor bearing mice of each treatment group. Data were expressed as mean ± SD, n = 10. (C) H E staining and Immunohistochemistry staining of S1PR1, pY-STAT3 and Ki67 of gastric tumor tissue removed from mice. Scale bar: 10 μM.

    Techniques Used: In Vivo, Mouse Assay, Staining, Immunohistochemistry

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    Abcam rabbit monoclonal antibodies against s1pr1
    Correlation of <t>S1PR1/STAT3</t> signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.
    Rabbit Monoclonal Antibodies Against S1pr1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibodies against s1pr1/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibodies against s1pr1 - by Bioz Stars, 2021-06
    99/100 stars
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    Correlation of S1PR1/STAT3 signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: Correlation of S1PR1/STAT3 signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques:

    Gastric cancer cell lines have different sensitivity to DDP treatment. (A) Cell viability of SGC7901 and SGC7901/DDP cells, cells were treated with fold diluted DDP. Data were expressed as mean ± SD, n = 4. (B) Cell viability of indicated gastric tumor cells, cells were treated with fold diluted DDP after transfection of S1PR1 overexpression or empty vector. Data were expressed as mean ± SD, n = 4. (C) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a progressive disease outcome. (D) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a complete remission/response outcome.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: Gastric cancer cell lines have different sensitivity to DDP treatment. (A) Cell viability of SGC7901 and SGC7901/DDP cells, cells were treated with fold diluted DDP. Data were expressed as mean ± SD, n = 4. (B) Cell viability of indicated gastric tumor cells, cells were treated with fold diluted DDP after transfection of S1PR1 overexpression or empty vector. Data were expressed as mean ± SD, n = 4. (C) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a progressive disease outcome. (D) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a complete remission/response outcome.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Transfection, Over Expression, Plasmid Preparation

    S1PR1 and pY-STAT3 were highly expressed in gastric cancer patients. (A) upper panel, Kaplan-Meier plots for overall survival of high and low transcript levels of S1PR1 in gastric cancer patients; lower panel transcript levels of S1PR1 in 4 stages of gastric cancer patients. (B) Immunohistochemistry staining of S1PR1 of gastric cancer patient tissues. Upper panel: a stage IIIb sample, lower panel: a stage IIa sample. Scale bar: 200 μM left panel, 100 μM right panel. (C) Immuno score levels of S1PR1 in different stage of gastric cancer patients were compared. (D) Correlation of S1PR1 and STAT3 transcript levels in gastric cancer patients. (E) Immunohistochemistry staining of pY-STAT3 of gastric cancer patient tissues. Upper panel stage IIIc, lower panel stage IIa Scale bar: 200 μM left panel, 100 μM right panel. (F) Correlation of S1PR1 and pY-STAT3 protein levels determined by IS in gastric cancer patients. (G) Left panel: Immunofluorescence double staining of S1PR1(green) and pY-STAT3(red) in gastric tumor, para-cancerous and normal samples, DAPI(blue) was adopted to reveal the nuclear of the cells. Scale bar: 20 μM. Right panel, quantification of patient tissue sections for percentages of overlapping red (pY-STAT3) and green (S1PR1) channels, shown as Manders colocalization coefficients M1 (p-STAT3 to S1PR1) and M2 (S1PR1 to p-STAT3), respectively.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: S1PR1 and pY-STAT3 were highly expressed in gastric cancer patients. (A) upper panel, Kaplan-Meier plots for overall survival of high and low transcript levels of S1PR1 in gastric cancer patients; lower panel transcript levels of S1PR1 in 4 stages of gastric cancer patients. (B) Immunohistochemistry staining of S1PR1 of gastric cancer patient tissues. Upper panel: a stage IIIb sample, lower panel: a stage IIa sample. Scale bar: 200 μM left panel, 100 μM right panel. (C) Immuno score levels of S1PR1 in different stage of gastric cancer patients were compared. (D) Correlation of S1PR1 and STAT3 transcript levels in gastric cancer patients. (E) Immunohistochemistry staining of pY-STAT3 of gastric cancer patient tissues. Upper panel stage IIIc, lower panel stage IIa Scale bar: 200 μM left panel, 100 μM right panel. (F) Correlation of S1PR1 and pY-STAT3 protein levels determined by IS in gastric cancer patients. (G) Left panel: Immunofluorescence double staining of S1PR1(green) and pY-STAT3(red) in gastric tumor, para-cancerous and normal samples, DAPI(blue) was adopted to reveal the nuclear of the cells. Scale bar: 20 μM. Right panel, quantification of patient tissue sections for percentages of overlapping red (pY-STAT3) and green (S1PR1) channels, shown as Manders colocalization coefficients M1 (p-STAT3 to S1PR1) and M2 (S1PR1 to p-STAT3), respectively.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Immunohistochemistry, Staining, Immunofluorescence, Double Staining

    S1PR1-STAT3 formed a positive regulatory loop in GC cells and contributed to drug resistance. (A) Expression of indicated genes of SGC7901/DDP cells after treated with different concentrations of S1P and DDP for 24 h was detected by Western blot. (B) Expression of indicated genes of SGC7901 cells transfected with S1PR1 over-expression and empty vectors for 48 h was detected by Western blot. (C) SGC7901 cells transfected with S1PR1 overexpression or empty vector cells were treated with fold diluted DDP for 24 h and the cell viability was detected by MTS method. Data were expressed as mean ± SD, n = 4. (D) Expression of indicated genes of SGC7901/DDP cells transfected with S1PR1 siRNA and mock siRNA for 48 h was determined by Western blot. (E) Cell viability of SGC7901/DDP cells, tested by MTS method. Cells were treated with fold diluted DDP for 24 h with or without S1PR1 knocking down. Data were expressed as mean ± SD, n = 4. (F) Cell apoptosis levels of SGC7091/DDP cells were determined by AnnexinV/PI double staining with FACS after indicated treatment for 24 h. The ratio of double negative population of the cells was presented with mean ± SD, n = 5. *P

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: S1PR1-STAT3 formed a positive regulatory loop in GC cells and contributed to drug resistance. (A) Expression of indicated genes of SGC7901/DDP cells after treated with different concentrations of S1P and DDP for 24 h was detected by Western blot. (B) Expression of indicated genes of SGC7901 cells transfected with S1PR1 over-expression and empty vectors for 48 h was detected by Western blot. (C) SGC7901 cells transfected with S1PR1 overexpression or empty vector cells were treated with fold diluted DDP for 24 h and the cell viability was detected by MTS method. Data were expressed as mean ± SD, n = 4. (D) Expression of indicated genes of SGC7901/DDP cells transfected with S1PR1 siRNA and mock siRNA for 48 h was determined by Western blot. (E) Cell viability of SGC7901/DDP cells, tested by MTS method. Cells were treated with fold diluted DDP for 24 h with or without S1PR1 knocking down. Data were expressed as mean ± SD, n = 4. (F) Cell apoptosis levels of SGC7091/DDP cells were determined by AnnexinV/PI double staining with FACS after indicated treatment for 24 h. The ratio of double negative population of the cells was presented with mean ± SD, n = 5. *P

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Expressing, Western Blot, Transfection, Over Expression, Plasmid Preparation, Double Staining, FACS

    Hyper expression of S1PR1 in gastric cancer cells identified drug resistance. (A) Transcript levels determined by QPCR and (B) protein expression levels determined by Western blot of indicated genes of SGC7901 and SGC7901/DDP gastric cancer cell lines. Data were expressed as mean ± SD, n = 5. *P

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: Hyper expression of S1PR1 in gastric cancer cells identified drug resistance. (A) Transcript levels determined by QPCR and (B) protein expression levels determined by Western blot of indicated genes of SGC7901 and SGC7901/DDP gastric cancer cell lines. Data were expressed as mean ± SD, n = 5. *P

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    FTY720 enhanced gastric tumor cells drug sensitivity in vivo . (A) Images of tumor bearing mice of each treatment group at the end of the experiment. (B) Body weight of mice of tumor bearing mice of each treatment group. Data were expressed as mean ± SD, n = 10. (C) H E staining and Immunohistochemistry staining of S1PR1, pY-STAT3 and Ki67 of gastric tumor tissue removed from mice. Scale bar: 10 μM.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: FTY720 enhanced gastric tumor cells drug sensitivity in vivo . (A) Images of tumor bearing mice of each treatment group at the end of the experiment. (B) Body weight of mice of tumor bearing mice of each treatment group. Data were expressed as mean ± SD, n = 10. (C) H E staining and Immunohistochemistry staining of S1PR1, pY-STAT3 and Ki67 of gastric tumor tissue removed from mice. Scale bar: 10 μM.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: In Vivo, Mouse Assay, Staining, Immunohistochemistry