phospho histone h2a x ser139 20e3 rabbit mab  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phospho histone h2a x ser139 20e3 rabbit mab
    a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of <t>γ-H2AX</t> staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14 + CD11b + healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V + cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.
    Phospho Histone H2a X Ser139 20e3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho histone h2a x ser139 20e3 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho histone h2a x ser139 20e3 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model"

    Article Title: Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model

    Journal: Nature Communications

    doi: 10.1038/s41467-024-48663-w

    a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of γ-H2AX staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14 + CD11b + healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V + cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.
    Figure Legend Snippet: a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of γ-H2AX staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14 + CD11b + healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V + cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.

    Techniques Used: Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay

    rabbit monoclonal anti phospho histone h2a x ser139 antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti phospho histone h2a x ser139 antibody
    Rabbit Monoclonal Anti Phospho Histone H2a X Ser139 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho histone h2a x ser139 antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho histone h2a x ser139 antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    phospho histone h2a x ser139 20e3 rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phospho histone h2a x ser139 20e3 rabbit mab
    Phospho Histone H2a X Ser139 20e3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho histone h2a x ser139 20e3 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho histone h2a x ser139 20e3 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

    Images

    phospho histone h2a x ser139 20e3 rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phospho histone h2a x ser139 20e3 rabbit mab
    a Viability assay of differentiated SH cells control treated for 72 hrs with either 10 μM Aβ40 or Aβ42 in the absence or presence of 10 μM ATA. Experiment was done in four technical replicates. b Western blot analysis of parental (Par) SH cells and three Ago2 k.o. clones. Experiments in a and b are representative of three independent biological repeats. c Western blot analysis of undifferentiated or 7-day differentiated SH cells and two Ago2 k.o. clones. d Viability assay of three Ago2 k.o. SH clones after exposure to 20 μM Aβ40 or Aβ42 for 72 hrs. Another k.o. clone was analyzed with similar results. This represents three independent experiments. Analysis of clones A9 and A10 is based on 5 technical replicates and of clone A11 on 8 technical replicates. P-values are given for the comparison of treated k.o. with parental cells in the same experiment. e , Top, Western blot analysis of differentiated SH cells control treated or pretreated with different concentrations of Enoxacin for 24 hrs and then treated with either 20 μM Aβ40 or Aβ42 for 24 hrs. This is representative of three independent experiments. Bottom, viability assay of the cells treated as above but for 72 hrs. This is based on 4 technical replicates. f Kinetics of <t>H2AX</t> phosphorylation of differentiated SH cells treated with 20 μM Aβ42. g Western blot analysis of differentiated SH cells first treated with 20 μM of Aβ40 or Aβ42 for 24 hrs and then control treated or treated with Enoxacin for 2 hours. In the sample on the right Aβ42 was washed out before an additional incubation for 2 hours. These data are representative of two independent experiments. h Western blot analysis of undifferentiated or 7-day differentiated parental SH cells or tau k.o. clone 231 K. i Viability assay of differentiated parental SH cells or two tau k.o. clones after mock treatment or treatment with 20 μM Aβ40/42. Experiment represents 4 technical replicates. This experiment was repeated with both differentiated and undifferentiated cells with similar results. j Western blot analysis of parental SH cells or different k.o. clones treated with 20 μM Aβ40/42 for 24 hrs. This is one of two independent experiments. k Western blot for γH2AX of 7 day differentiated SH cells treated with Aβ40 or Aβ42 for four hours in the absence or pretreated for 24 hrs with of 10 μM ATA. This experiment was repeated twice with different ATA concentrations. Mean with SD and two-sided Student’s t-test p-values are shown ( a, d, i ).
    Phospho Histone H2a X Ser139 20e3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho histone h2a x ser139 20e3 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho histone h2a x ser139 20e3 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Death Induced by Survival gene Elimination (DISE) correlates with neurotoxicity in Alzheimer’s disease and aging"

    Article Title: Death Induced by Survival gene Elimination (DISE) correlates with neurotoxicity in Alzheimer’s disease and aging

    Journal: Nature Communications

    doi: 10.1038/s41467-023-44465-8

    a Viability assay of differentiated SH cells control treated for 72 hrs with either 10 μM Aβ40 or Aβ42 in the absence or presence of 10 μM ATA. Experiment was done in four technical replicates. b Western blot analysis of parental (Par) SH cells and three Ago2 k.o. clones. Experiments in a and b are representative of three independent biological repeats. c Western blot analysis of undifferentiated or 7-day differentiated SH cells and two Ago2 k.o. clones. d Viability assay of three Ago2 k.o. SH clones after exposure to 20 μM Aβ40 or Aβ42 for 72 hrs. Another k.o. clone was analyzed with similar results. This represents three independent experiments. Analysis of clones A9 and A10 is based on 5 technical replicates and of clone A11 on 8 technical replicates. P-values are given for the comparison of treated k.o. with parental cells in the same experiment. e , Top, Western blot analysis of differentiated SH cells control treated or pretreated with different concentrations of Enoxacin for 24 hrs and then treated with either 20 μM Aβ40 or Aβ42 for 24 hrs. This is representative of three independent experiments. Bottom, viability assay of the cells treated as above but for 72 hrs. This is based on 4 technical replicates. f Kinetics of H2AX phosphorylation of differentiated SH cells treated with 20 μM Aβ42. g Western blot analysis of differentiated SH cells first treated with 20 μM of Aβ40 or Aβ42 for 24 hrs and then control treated or treated with Enoxacin for 2 hours. In the sample on the right Aβ42 was washed out before an additional incubation for 2 hours. These data are representative of two independent experiments. h Western blot analysis of undifferentiated or 7-day differentiated parental SH cells or tau k.o. clone 231 K. i Viability assay of differentiated parental SH cells or two tau k.o. clones after mock treatment or treatment with 20 μM Aβ40/42. Experiment represents 4 technical replicates. This experiment was repeated with both differentiated and undifferentiated cells with similar results. j Western blot analysis of parental SH cells or different k.o. clones treated with 20 μM Aβ40/42 for 24 hrs. This is one of two independent experiments. k Western blot for γH2AX of 7 day differentiated SH cells treated with Aβ40 or Aβ42 for four hours in the absence or pretreated for 24 hrs with of 10 μM ATA. This experiment was repeated twice with different ATA concentrations. Mean with SD and two-sided Student’s t-test p-values are shown ( a, d, i ).
    Figure Legend Snippet: a Viability assay of differentiated SH cells control treated for 72 hrs with either 10 μM Aβ40 or Aβ42 in the absence or presence of 10 μM ATA. Experiment was done in four technical replicates. b Western blot analysis of parental (Par) SH cells and three Ago2 k.o. clones. Experiments in a and b are representative of three independent biological repeats. c Western blot analysis of undifferentiated or 7-day differentiated SH cells and two Ago2 k.o. clones. d Viability assay of three Ago2 k.o. SH clones after exposure to 20 μM Aβ40 or Aβ42 for 72 hrs. Another k.o. clone was analyzed with similar results. This represents three independent experiments. Analysis of clones A9 and A10 is based on 5 technical replicates and of clone A11 on 8 technical replicates. P-values are given for the comparison of treated k.o. with parental cells in the same experiment. e , Top, Western blot analysis of differentiated SH cells control treated or pretreated with different concentrations of Enoxacin for 24 hrs and then treated with either 20 μM Aβ40 or Aβ42 for 24 hrs. This is representative of three independent experiments. Bottom, viability assay of the cells treated as above but for 72 hrs. This is based on 4 technical replicates. f Kinetics of H2AX phosphorylation of differentiated SH cells treated with 20 μM Aβ42. g Western blot analysis of differentiated SH cells first treated with 20 μM of Aβ40 or Aβ42 for 24 hrs and then control treated or treated with Enoxacin for 2 hours. In the sample on the right Aβ42 was washed out before an additional incubation for 2 hours. These data are representative of two independent experiments. h Western blot analysis of undifferentiated or 7-day differentiated parental SH cells or tau k.o. clone 231 K. i Viability assay of differentiated parental SH cells or two tau k.o. clones after mock treatment or treatment with 20 μM Aβ40/42. Experiment represents 4 technical replicates. This experiment was repeated with both differentiated and undifferentiated cells with similar results. j Western blot analysis of parental SH cells or different k.o. clones treated with 20 μM Aβ40/42 for 24 hrs. This is one of two independent experiments. k Western blot for γH2AX of 7 day differentiated SH cells treated with Aβ40 or Aβ42 for four hours in the absence or pretreated for 24 hrs with of 10 μM ATA. This experiment was repeated twice with different ATA concentrations. Mean with SD and two-sided Student’s t-test p-values are shown ( a, d, i ).

    Techniques Used: Viability Assay, Western Blot, Clone Assay, Comparison, Incubation

    phospho histone h2a x ser139 20e3 rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phospho histone h2a x ser139 20e3 rabbit mab
    Phospho Histone H2a X Ser139 20e3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho histone h2a x ser139 20e3 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho histone h2a x ser139 20e3 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

    Images

    rabbit monoclonal anti phospho histone h2a x ser139  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti phospho histone h2a x ser139
    Overview of the impact of melatonin administration on key skin aging biomarkers. ↑ = increased, ↔ = no change, ↓ = decreased, compared to the vehicle. Mann–Whitney or Student’s t -test, * p < 0.5, ** p < 0.01.
    Rabbit Monoclonal Anti Phospho Histone H2a X Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho histone h2a x ser139/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho histone h2a x ser139 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo"

    Article Title: Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms242115963

    Overview of the impact of melatonin administration on key skin aging biomarkers. ↑ = increased, ↔ = no change, ↓ = decreased, compared to the vehicle. Mann–Whitney or Student’s t -test, * p < 0.5, ** p < 0.01.
    Figure Legend Snippet: Overview of the impact of melatonin administration on key skin aging biomarkers. ↑ = increased, ↔ = no change, ↓ = decreased, compared to the vehicle. Mann–Whitney or Student’s t -test, * p < 0.5, ** p < 0.01.

    Techniques Used:

    List of antibodies and immunohistochemical staining treatments used in the study.
    Figure Legend Snippet: List of antibodies and immunohistochemical staining treatments used in the study.

    Techniques Used: Immunohistochemistry, Staining, Blocking Assay, Amplification, Avidin-Biotin Assay

    rabbit monoclonal anti phospho histone h2a x ser139  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti phospho histone h2a x ser139
    KEY RESOURCES TABLE
    Rabbit Monoclonal Anti Phospho Histone H2a X Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho histone h2a x ser139/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho histone h2a x ser139 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Irreversible cell cycle exit associated with senescence is mediated by constitutive MYC degradation"

    Article Title: Irreversible cell cycle exit associated with senescence is mediated by constitutive MYC degradation

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.113079

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Luminex, Staining, shRNA, Software, Imaging

    rabbit monoclonal anti phospho histone h2a x ser139  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti phospho histone h2a x ser139

    Rabbit Monoclonal Anti Phospho Histone H2a X Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho histone h2a x ser139/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho histone h2a x ser139 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "WNK1 is required during male pachynema to sustain fertility"

    Article Title: WNK1 is required during male pachynema to sustain fertility

    Journal: iScience

    doi: 10.1016/j.isci.2023.107616


    Figure Legend Snippet:

    Techniques Used: Recombinant, In Situ, Software

    rabbit monoclonal anti phospho histone h2a x ser139  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti phospho histone h2a x ser139
    Key Resources Table
    Rabbit Monoclonal Anti Phospho Histone H2a X Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho histone h2a x ser139/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho histone h2a x ser139 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "SPARCLE , a p53-induced lncRNA, controls apoptosis after genotoxic stress by promoting PARP-1 cleavage"

    Article Title: SPARCLE , a p53-induced lncRNA, controls apoptosis after genotoxic stress by promoting PARP-1 cleavage

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2022.01.001

    Key Resources Table
    Figure Legend Snippet: Key Resources Table

    Techniques Used: Recombinant, Binding Assay, Modification, Protease Inhibitor, Immunoprecipitation, Hybridization, Staining, Labeling, TUNEL Assay, Caspase-Glo Assay, PCR Cloning, Luciferase, Reporter Assay, Purification, Gel Extraction, Plasmid Preparation, Western Blot, Microscopy, Negative Control, Software, Imaging

    phospho histone h2a x ser139 20e3 rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phospho histone h2a x ser139 20e3 rabbit mab
    Phospho Histone H2a X Ser139 20e3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho histone h2a x ser139 20e3 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho histone h2a x ser139 20e3 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Cell Signaling Technology Inc phospho histone h2a x ser139 20e3 rabbit mab
    a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of <t>γ-H2AX</t> staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14 + CD11b + healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V + cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.
    Phospho Histone H2a X Ser139 20e3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho histone h2a x ser139 20e3 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho histone h2a x ser139 20e3 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc rabbit monoclonal anti phospho histone h2a x ser139 antibody
    a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of <t>γ-H2AX</t> staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14 + CD11b + healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V + cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.
    Rabbit Monoclonal Anti Phospho Histone H2a X Ser139 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho histone h2a x ser139 antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho histone h2a x ser139 antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc rabbit monoclonal anti phospho histone h2a x ser139
    Overview of the impact of melatonin administration on key skin aging biomarkers. ↑ = increased, ↔ = no change, ↓ = decreased, compared to the vehicle. Mann–Whitney or Student’s t -test, * p < 0.5, ** p < 0.01.
    Rabbit Monoclonal Anti Phospho Histone H2a X Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho histone h2a x ser139/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho histone h2a x ser139 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of γ-H2AX staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14 + CD11b + healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V + cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model

    doi: 10.1038/s41467-024-48663-w

    Figure Lengend Snippet: a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of γ-H2AX staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14 + CD11b + healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V + cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.

    Article Snippet: Cells were incubated with phospho-histone H2A.X (Ser139) (20E3) rabbit mAb (cat. #9718, Cell Signaling) diluted 1:200 in blocking buffer at 4 °C overnight on a rotator.

    Techniques: Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay

    Overview of the impact of melatonin administration on key skin aging biomarkers. ↑ = increased, ↔ = no change, ↓ = decreased, compared to the vehicle. Mann–Whitney or Student’s t -test, * p < 0.5, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo

    doi: 10.3390/ijms242115963

    Figure Lengend Snippet: Overview of the impact of melatonin administration on key skin aging biomarkers. ↑ = increased, ↔ = no change, ↓ = decreased, compared to the vehicle. Mann–Whitney or Student’s t -test, * p < 0.5, ** p < 0.01.

    Article Snippet: , γH2A.x , 4% PFA, 10 min at RT , 10% goat serum in PBS , rabbit monoclonal anti-phospho-histone H2A.X (Ser139) (γH2A.X) (1:1500; Cell Signaling, #2577S) , goat monoclonal anti-rabbit IgG-Alexa Fluor 555 (1:400; Invitrogen, A21428).

    Techniques:

    List of antibodies and immunohistochemical staining treatments used in the study.

    Journal: International Journal of Molecular Sciences

    Article Title: Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo

    doi: 10.3390/ijms242115963

    Figure Lengend Snippet: List of antibodies and immunohistochemical staining treatments used in the study.

    Article Snippet: , γH2A.x , 4% PFA, 10 min at RT , 10% goat serum in PBS , rabbit monoclonal anti-phospho-histone H2A.X (Ser139) (γH2A.X) (1:1500; Cell Signaling, #2577S) , goat monoclonal anti-rabbit IgG-Alexa Fluor 555 (1:400; Invitrogen, A21428).

    Techniques: Immunohistochemistry, Staining, Blocking Assay, Amplification, Avidin-Biotin Assay