Journal: International Journal of Molecular Sciences

Article Title: Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo

doi: 10.3390/ijms242115963

Figure Lengend Snippet: Overview of the impact of melatonin administration on key skin aging biomarkers. ↑ = increased, ↔ = no change, ↓ = decreased, compared to the vehicle. Mann–Whitney or Student’s t -test, * p < 0.5, ** p < 0.01.

Article Snippet: , γH2A.x , 4% PFA, 10 min at RT , 10% goat serum in PBS , rabbit monoclonal anti-phospho-histone H2A.X (Ser139) (γH2A.X) (1:1500; Cell Signaling, #2577S) , goat monoclonal anti-rabbit IgG-Alexa Fluor 555 (1:400; Invitrogen, A21428).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo

doi: 10.3390/ijms242115963

Figure Lengend Snippet: List of antibodies and immunohistochemical staining treatments used in the study.

Article Snippet: , γH2A.x , 4% PFA, 10 min at RT , 10% goat serum in PBS , rabbit monoclonal anti-phospho-histone H2A.X (Ser139) (γH2A.X) (1:1500; Cell Signaling, #2577S) , goat monoclonal anti-rabbit IgG-Alexa Fluor 555 (1:400; Invitrogen, A21428).

Techniques: Immunohistochemistry, Staining, Blocking Assay, Amplification, Avidin-Biotin Assay

Journal: International Journal of Molecular Sciences

Article Title: Less Severe Polymicrobial Sepsis in Conditional mgmt -Deleted Mice Using LysM-Cre System, Impacts of DNA Methylation and MGMT Inhibitor in Sepsis

doi: 10.3390/ijms241210175

Figure Lengend Snippet: The characteristics of bone marrow-derived macrophages (BMDM) from mgmt control (mgmt fl/fl ; LysM-Cre -/- ) or mgmt null (mgmt fl/fl ; LysM-Cre cre/- ) mice at 24 h after activation by lipopolysaccharide (LPS) as indicated by supernatant cell-free DNA ( A ), malondialdehyde (MDA; a reactive stress molecule) ( B ), and immunofluorescent stained for DNA break (phospho-histone H2A.X; green color) and actin filament (red color) in intensity score and representative pictures ( C , D ) are demonstrated. Triplicated independent experiments were performed. Mean ± SEM with the one-way ANOVA followed by Tukey’s analysis was used. #, p ˂ 0.05 vs mgmt control DMEM; *, p ˂ 0.05 between the indicated groups.

Article Snippet: Fixed samples were blocked with 2% bovine serum albumin in 1X TBS for 1 h at room temperature and then incubated overnight at 4 °C with phospho-histone H2A.X (Ser139) (20E3) rabbit mAb (Cell signaling).

Techniques: Derivative Assay, Activation Assay, Staining

Journal: bioRxiv

Article Title: The RNA helicase DDX1 associates with the nuclear RNA exosome and modulates R-loops

doi: 10.1101/2023.04.17.537228

Figure Lengend Snippet: (A) N2A cells were treated with camptothecin (CPT) or PBS (Control), fixed, and analyzed by immunofluorescence using an antibody that detects the DNA damage marker, γH2AX. (B) The Input and immunoprecipitated samples from nuclear fractions (Bound) treated with either CPT or PBS (Control) for both EXOSC3 and control IgG (Ctrl IgG) are shown. DDX1, EXOSC9, and EXOSC3 are detected. (C) The immunoprecipitation experiment in was performed in biological triplicate and DDX1 bands in EXOSC3 Bound fractions were quantified. Statistical significance was calculated by a student’s T-test. Asterisk (*) represents p-value < 0.05. IP: immunoprecipitation; IB: immunoblot.

Article Snippet: Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400).

Techniques: Immunofluorescence, Marker, Immunoprecipitation, Western Blot