phospho akt ser473 d9e xp rabbit mab 4060 cell signaling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473 d9e xp rabbit mab 4060 cell signaling
    sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt <t>(Ser473),</t> and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.
    Phospho Akt Ser473 D9e Xp Rabbit Mab 4060 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473 d9e xp rabbit mab 4060 cell signaling/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    phospho akt ser473 d9e xp rabbit mab 4060 cell signaling - by Bioz Stars, 2024-07
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    1) Product Images from "Soluble CD72, is a T-cell activator probably via binding to CD6 in homeostasis and autoimmunity"

    Article Title: Soluble CD72, is a T-cell activator probably via binding to CD6 in homeostasis and autoimmunity

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1367120

    sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt (Ser473), and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.
    Figure Legend Snippet: sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt (Ser473), and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.

    Techniques Used: Binding Assay, Western Blot

    anti phospho akt ½ ser473 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho akt ½ ser473 rabbit mab
    Anti Phospho Akt ½ Ser473 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho akt ½ ser473 rabbit mab/product/Cell Signaling Technology Inc
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    phospho akt ser473 rabbit mab rabbit mab 60 kda  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473 rabbit mab rabbit mab 60 kda
    Phospho Akt Ser473 Rabbit Mab Rabbit Mab 60 Kda, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473 rabbit mab rabbit mab 60 kda/product/Cell Signaling Technology Inc
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    rabbit monoclonal anti phospho akt ser473  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt ser473

    Rabbit Monoclonal Anti Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt ser473/product/Cell Signaling Technology Inc
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    1) Product Images from "Cbl and Cbl-b ubiquitin ligases are essential for intestinal epithelial stem cell maintenance"

    Article Title: Cbl and Cbl-b ubiquitin ligases are essential for intestinal epithelial stem cell maintenance

    Journal: iScience

    doi: 10.1016/j.isci.2024.109912


    Figure Legend Snippet:

    Techniques Used: Recombinant, In Vivo, In Vitro, Plasmid Preparation, Staining, Polymer, Blocking Assay, Amplification, Isolation, Reverse Transcription, SYBR Green Assay, Western Blot, Bicinchoninic Acid Protein Assay, Knock-In, Control, Software, Flow Cytometry

    anti phospho akt ser473 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho akt ser473 rabbit mab
    Anti Phospho Akt Ser473 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho akt ser473 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho akt ser473 rabbit mab - by Bioz Stars, 2024-07
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    rabbit monoclonal anti phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt ser473
    List of the antibodies with relative concentration used.
    Rabbit Monoclonal Anti Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt ser473/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    rabbit monoclonal anti phospho akt ser473 - by Bioz Stars, 2024-07
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    1) Product Images from "Expression and functional significance of phosphoenolpyruvate carboxykinase 1 in uveal melanoma"

    Article Title: Expression and functional significance of phosphoenolpyruvate carboxykinase 1 in uveal melanoma

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-024-01963-y

    List of the antibodies with relative concentration used.
    Figure Legend Snippet: List of the antibodies with relative concentration used.

    Techniques Used: Concentration Assay

    rabbit monoclonal anti phospho akt ser473  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt ser473
    Rabbit Monoclonal Anti Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt ser473/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    rabbit monoclonal anti phospho akt ser473 - by Bioz Stars, 2024-07
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    rabbit monoclonal anti phospho akt xp ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt xp ser473
    (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and <t>Ser473</t> phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.
    Rabbit Monoclonal Anti Phospho Akt Xp Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt xp ser473/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    rabbit monoclonal anti phospho akt xp ser473 - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1"

    Article Title: Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.113837

    (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and Ser473 phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.
    Figure Legend Snippet: (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and Ser473 phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.

    Techniques Used: Expressing, RNA Sequencing Assay, Western Blot, Immunoprecipitation, Activation Assay


    Figure Legend Snippet:

    Techniques Used: Virus, Expressing, Plasmid Preparation, Recombinant, Isolation, MTT Assay, Western Blot, Software

    phospho akt ser473 d9e xp rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc phospho akt ser473 d9e xp rabbit mab

    Phospho Akt Ser473 D9e Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473 d9e xp rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 d9e xp rabbit mab - by Bioz Stars, 2024-07
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    1) Product Images from "Glucose starvation causes ferroptosis-mediated lysosomal dysfunction"

    Article Title: Glucose starvation causes ferroptosis-mediated lysosomal dysfunction

    Journal: iScience

    doi: 10.1016/j.isci.2024.109735


    Figure Legend Snippet:

    Techniques Used: Recombinant, Produced, Activity Assay, Modification, Protease Inhibitor, Blocking Assay, Software, Real-time Polymerase Chain Reaction

    rabbit monoclonal anti phospho akt xp ser473  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt xp ser473
    (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and <t>Ser473</t> phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.
    Rabbit Monoclonal Anti Phospho Akt Xp Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt xp ser473/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    rabbit monoclonal anti phospho akt xp ser473 - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1"

    Article Title: Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.113837

    (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and Ser473 phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.
    Figure Legend Snippet: (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and Ser473 phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.

    Techniques Used: Expressing, RNA Sequencing Assay, Western Blot, Immunoprecipitation, Activation Assay


    Figure Legend Snippet:

    Techniques Used: Virus, Expressing, Plasmid Preparation, Recombinant, Isolation, MTT Assay, Western Blot, Software

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    Cell Signaling Technology Inc phospho akt ser473 d9e xp rabbit mab 4060 cell signaling
    sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt <t>(Ser473),</t> and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.
    Phospho Akt Ser473 D9e Xp Rabbit Mab 4060 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti phospho akt ½ ser473 rabbit mab
    sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt <t>(Ser473),</t> and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.
    Anti Phospho Akt ½ Ser473 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc phospho akt ser473 rabbit mab rabbit mab 60 kda
    sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt <t>(Ser473),</t> and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.
    Phospho Akt Ser473 Rabbit Mab Rabbit Mab 60 Kda, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Rabbit Monoclonal Anti Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt ser473/product/Cell Signaling Technology Inc
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    Anti Phospho Akt Ser473 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho akt ser473 rabbit mab/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt xp ser473
    (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and <t>Ser473</t> phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.
    Rabbit Monoclonal Anti Phospho Akt Xp Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt xp ser473/product/Cell Signaling Technology Inc
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    rabbit monoclonal anti phospho akt xp ser473 - by Bioz Stars, 2024-07
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    Cell Signaling Technology Inc phospho akt ser473 d9e xp rabbit mab

    Phospho Akt Ser473 D9e Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473 d9e xp rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 d9e xp rabbit mab - by Bioz Stars, 2024-07
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    sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt (Ser473), and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.

    Journal: Frontiers in Immunology

    Article Title: Soluble CD72, is a T-cell activator probably via binding to CD6 in homeostasis and autoimmunity

    doi: 10.3389/fimmu.2024.1367120

    Figure Lengend Snippet: sCD72 binding to CD6 promotes the phosphorylation of SLP-76 (Ser 376), Akt (Ser 473), and mTOR (Ser2448) in CD4 + T cells Activated CD4 + T cells were stimulated with 2-µg/ml sCD72 for 10 min on ice followed by 5 min at 37°C. Then, cell lysates were prepared in the presence of a phosphorylation buffer, as described. Blots prepared from the lysates were probed with antibodies directed against (A) Phospho-SLP-76 (Ser 376), (B) Phospho-Akt (Ser473), and (C) Phospho-mTOR (Ser2448). Shown are the resulting western blots and the quantification graphs, which represent the mean ± SD of the phospho/total ratio of each indicated component from 6 independent experiments. P, phosphor; T, total; *,P<0.05.

    Article Snippet: Phospho-AKT (Ser473) (D9E) XP ® Rabbit mAb (4060, Cell Signaling).

    Techniques: Binding Assay, Western Blot

    Journal: iScience

    Article Title: Cbl and Cbl-b ubiquitin ligases are essential for intestinal epithelial stem cell maintenance

    doi: 10.1016/j.isci.2024.109912

    Figure Lengend Snippet:

    Article Snippet: Rabbit Monoclonal anti-Phospho AKT (Ser473) , Cell Signaling Technology , Cat#4060; RRID: AB_2315049.

    Techniques: Recombinant, In Vivo, In Vitro, Plasmid Preparation, Staining, Polymer, Blocking Assay, Amplification, Isolation, Reverse Transcription, SYBR Green Assay, Western Blot, Bicinchoninic Acid Protein Assay, Knock-In, Control, Software, Flow Cytometry

    (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and Ser473 phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.

    Journal: Cell reports

    Article Title: Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1

    doi: 10.1016/j.celrep.2024.113837

    Figure Lengend Snippet: (A) The gene expression levels in HUVECs were compared between Akt isoforms by RNA-seq. N = 3. Data are represented as the mean ± SD. (B and C) The protein level of each Akt isoform (N = 3–5) and Ser473 phosphorylation of all Akts were determined in R-Ras38V-transduced (B) or R-Ras-silenced (C) ECs by western blot. For quantification, pAkt Ser473 was normalized to the total Akt. Data are represented as the mean ± SEM. (D) Phosphorylation of each Akt isoform was determined in mock- or R-Ras38V-transduced ECs by immunoprecipitation of the total pAkt Ser473 followed by western blot of each isoform. (E) Each Akt isoform was silenced in mock- or R-Ras38V-transduced ECs and the protein levels of Jagged1 and p21 were determined in these cells. (F) The Akt3 isoform was silenced in R-Ras38V-transduced ECs, and Notch1 and Notch3 activation as well as the protein levels of Jagged1, p21, VE-cadherin, and UNC5b were determined. Data are represented as the mean ± SEM. (G) Akt3 was silenced in mock- (EC mock ) or R-Ras38V-transduced ECs (EC RRas38V ) that were cocultured with parental ECs (EC), and Jagged1 expression was evaluated. *p < 0.05, **p < 0.01; n.s., not significant.

    Article Snippet: Rabbit monoclonal anti-phospho Akt XP (Ser473) (D9E) , Cell Signaling Technology , Cat #4060; RRID:AB_2315049.

    Techniques: Expressing, RNA Sequencing Assay, Western Blot, Immunoprecipitation, Activation Assay

    Journal: Cell reports

    Article Title: Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1

    doi: 10.1016/j.celrep.2024.113837

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal anti-phospho Akt XP (Ser473) (D9E) , Cell Signaling Technology , Cat #4060; RRID:AB_2315049.

    Techniques: Virus, Expressing, Plasmid Preparation, Recombinant, Isolation, MTT Assay, Western Blot, Software

    Journal: iScience

    Article Title: Glucose starvation causes ferroptosis-mediated lysosomal dysfunction

    doi: 10.1016/j.isci.2024.109735

    Figure Lengend Snippet:

    Article Snippet: Phospho-Akt (Ser473) (D9E) XP Rabbit mAb , Cell Signaling Technology , #4060.

    Techniques: Recombinant, Produced, Activity Assay, Modification, Protease Inhibitor, Blocking Assay, Software, Real-time Polymerase Chain Reaction