Journal: Experimental and Therapeutic Medicine

Article Title: Gelatin methacryloyl hydrogel scaffold loaded with activated Schwann cells attenuates apoptosis and promotes functional recovery following spinal cord injury

doi: 10.3892/etm.2023.11843

Figure Lengend Snippet: Apoptosis-related proteins and pathways detected using western blot analysis. (A and B) Representative western blots and quantification of expression levels for protein caspase-3 and Bcl-2 in each group at 7 days post-surgery. (C and D) Detection of apoptosis-related pathways, including p38, ERK1/2, and JNK1/2. The data shown are the mean ± standard deviation (n=6 in each group). *P<0.05 and **P<0.01 compared with the SCI or ASC group. ASCs, activated Schwann cells; SCI, spinal cord injury; ns, no significance.

Article Snippet: The primary antibodies used were as follows: Anti-Bcl-2 (1:1,000; cat. no. ab196495; Abcam), anti-caspase-3 (1:1,000; cat. no. ab32351; Abcam), anti-p38 (1:1,000; cat. no. 8690; Cell Signaling Technology, Inc.), anti-p-p38 (1:1,000; cat. no. 4511; Cell Signaling Technology, Inc.), anti-ERK1/2 (1:1,000; cat. no. 9194; Cell Signaling Technology, Inc.), anti-p-ERK1/2 (1:1,000; cat. no. 4370; Cell Signaling Technology, Inc.), anti-JNK1/2 (1:1,000; cat. no. 3708; Cell Signaling Technology, Inc.), anti-p-JNK1/2 (1:1,000, cat. no. 9255, Cell Signaling Technology, Inc.), and anti-GAPDH (1:1,000, cat. no. AF0006, Beyotime Institute of Biotechnology).

Techniques: Western Blot, Expressing, Standard Deviation