anti gfp d5 1 mab rabbit (Cell Signaling Technology Inc)


Structured Review

Anti Gfp D5 1 Mab Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gfp d5 1 mab rabbit/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Transcriptional kinetic synergy: A complex landscape revealed by integrating modeling and synthetic biology"
Article Title: Transcriptional kinetic synergy: A complex landscape revealed by integrating modeling and synthetic biology
Journal: Cell systems
doi: 10.1016/j.cels.2023.02.003

Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Recombinant, SYBR Green Assay, Plasmid Preparation, Software, Flow Cytometry
anti gfp d5 1 rabbit monoclonal antibody (Cell Signaling Technology Inc)


Structured Review

Anti Gfp D5 1 Rabbit Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gfp d5 1 rabbit monoclonal antibody/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Transcriptional kinetic synergy: A complex landscape revealed by integrating modeling and synthetic biology"
Article Title: Transcriptional kinetic synergy: A complex landscape revealed by integrating modeling and synthetic biology
Journal: Cell systems
doi: 10.1016/j.cels.2023.02.003

Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Recombinant, SYBR Green Assay, Plasmid Preparation, Software, Flow Cytometry
gfp d5 1 rabbit mab (Cell Signaling Technology Inc)


Structured Review
Gfp D5 1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp d5 1 rabbit mab/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti gfp d5 1 xp rabbit mab (Cell Signaling Technology Inc)


Structured Review
Anti Gfp D5 1 Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gfp d5 1 xp rabbit mab/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
gfp d5 1 rabbit mab (Cell Signaling Technology Inc)


Structured Review

Gfp D5 1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp d5 1 rabbit mab/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia"
Article Title: Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia
Journal: iScience
doi: 10.1016/j.isci.2023.107024

Figure Legend Snippet: Identification of EDRF1 as another substrate of dengue virus protease (A and B) In vitro pull-down assay from K562 cell lysate with pRSET-A NS2BNS3pro purified protein and (B) negative control. The arrows indicate the bands used for identification. See also
Techniques Used: In Vitro, Pull Down Assay, Purification, Negative Control, Western Blot, Cotransfection, Transfection, Recombinant, Staining, Marker

Figure Legend Snippet:
Techniques Used: Infection, Recombinant, Staining, Plasmid Preparation, Isolation, Gel Extraction, Derivative Assay, Software
rabbit anti gfp monoclonal antibody (Cell Signaling Technology Inc)


Structured Review
Rabbit Anti Gfp Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti gfp monoclonal antibody/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti gfp d5 1 xp rabbit mab (Cell Signaling Technology Inc)


Structured Review

Anti Gfp D5 1 Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gfp d5 1 xp rabbit mab/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells"
Article Title: Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells
Journal: Journal of Cell Science
doi: 10.1242/jcs.260827

Figure Legend Snippet: Pacsin 2 SH3 domain interacts with the N-cadherin cytoplasmic region to regulate cell–cell contact formation. (A) Schematically illustrated domain structures of human pacsin 2 and human N-cadherin. Cyto, cytoplasmic domain; EC, extracellular domain; TM, transmembrane domain. Numbers indicate amino acid positions. (B) Amino acid sequences of the cytoplasmic domain of N-cadherin. Two PxxP motifs (bold and underlined) and p120-binding regions (italicized and underlined) are shown. Numbers indicate amino acid positions. (C) Interaction between pacsin 2 SH3 domain and N-cadherin in T24 cells in GST pull-down assays. Immunoblot probed with anti-N-cadherin antibody (IB: αNCAD) and a CBB-stained SDS–PAGE gel (CBB) for input (Inp; 0.16% of total lysate) and pulled-down fractions with either GST beads (GST) or GST–pacsin 2 SH3 beads (GST–SH3) are shown. The positions of endogenous N-cadherin in T24 cells, and of GST–SH3 and GST alone, are marked by arrowheads. (D) Interaction between pacsin 2 SH3 domain and N-cadherin cytoplasmic domain in GST pull-down assays. Immunoblots probed with anti-GFP antibody (IB: αGFP) and CBB-stained SDS–PAGE gels (CBB) for input (Inp; 0.16% of total lysate) and pulled-down fractions with either GST beads (GST) or GST–pacsin 2 SH3 beads (GST–SH3) are shown. Positions of wild-type (wt) and mutant (P818/821A, P847/850/851A and P818/821/847/850/851A) forms of GFP-tagged N-cadherin cytoplasmic domain (GFP–NCADcyto), and of GST (GST) and GST-tagged pacsin 2 SH3 domain (GST–SH3), are marked by arrowheads. (E) Interaction between pacsin 2 SH3 domain and full-length N-cadherin in GST pull-down assays. Immunoblots probed with anti-GFP antibody (IB: αGFP) and CBB-stained SDS–PAGE gels (CBB) for input (Inp; 0.16% of total lysate) and pulled-down fractions with either GST beads (GST) or GST–pacsin 2 SH3 beads (GST–SH3) are shown. Positions of wild-type (Wt) or the PxxP mutant form (P818/821/847/850/851A) of GFP-tagged N-cadherin (NCAD–GFP), and of GST (GST) and GST-tagged pacsin 2 SH3 domain (GST–SH3), are marked by arrowheads. Images shown in C–E are representative of three independent experiments. (F) Expression of pacsin 2-binding-defective N-cadherin phenocopies the effects of pacsin 2 depletion. Immunofluorescence images showing exogenously expressed GFP-tagged wild-type (NCADWt–GFP) or PA mutant (P818/821/847/850/851A mutations; NCADPA–GFP) N-cadherin, and F-actin in T24 cells. Merged images show GFP-tagged N-cadherin (green), F-actin (red) and DNA (blue). Scale bar: 10 μm. (G) Expression of pacsin 2-binding-defective N-cadherin induces accumulations of a junctional component. Immunofluorescence images showing exogenously expressed GFP-tagged wild-type (NCADWt–GFP) or PA mutant (NCADPA–GFP) N-cadherin, and α-catenin in T24 cells. Merged images show GFP-tagged N-cadherin (green), α-catenin (red) and DNA (blue). Scale bar: 10 μm. (H) Quantification of GFP-tagged N-cadherin (NCAD–GFP) signal intensity at cell–cell contact sites for wild-type (Wt) and PA mutant (PA) (Wt, n =63; PA, n =105). (I) Quantification of α-catenin signal intensity at cell–cell contact sites in cells expressing either wild-type (Wt) or PA mutant (PA) NCAD–GFP (Wt, n =63; PA, n =105). (J) The relative intensity of either wild-type (Wt) or PA mutant (PA) NCAD–GFP at cell–cell contact sites, normalized to α-catenin intensity (Wt, n =63; PA, n =105). Horizontal lines in H–J indicate the median. P -values in H–J were calculated using a two-tailed Mann–Whitney test (ns, not significant). (K) Quantification of cell contact formation upon expression of either wild-type (Wt) or PA mutant (PA) NCAD–GFP. Data are presented as mean±s.d. ( n ≥110 cells, N =3). P -values were calculated using an unpaired two-tailed t -test.
Techniques Used: Binding Assay, Western Blot, Staining, SDS Page, Mutagenesis, Expressing, Immunofluorescence, Two Tailed Test, MANN-WHITNEY

Figure Legend Snippet: A N-cadherin mutant with defective pacsin 2 binding shows attenuated internalization. (A) Representative immunoblots of N-cadherin internalization experiments. Surface GFP-tagged N-cadherin was biotinylated at 4°C, and cells were subsequently incubated at 37°C for the indicated periods to allow endocytosis. MESNa was added, as indicated, to remove biotin remaining at the cell surface. Immunoblots using an anti-GFP antibody (IB: αGFP) to detect total GFP-tagged N-cadherin (Input, arrowheads) and internalized GFP-tagged N-cadherin (Pull-down, arrowheads) from T24 cells expressing either wild-type (NCADWt–GFP) or PA mutant (NCADPA–GFP) N-cadherin are shown. (B) Quantification of internalized GFP-tagged N-cadherin from cells expressing either wild-type (NCADWt–GFP) or PA mutant (NCADPA–GFP) N-cadherin after normalizing the internalized GFP-tagged N-cadherin (Pull-down) to the total amount of GFP-tagged N-cadherin (Input) in experiments as shown in A. Data are mean±s.d. ( n =3).
Techniques Used: Mutagenesis, Binding Assay, Western Blot, Incubation, Expressing
rabbit monoclonal anti gfp (Cell Signaling Technology Inc)


Structured Review
Rabbit Monoclonal Anti Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal anti gfp/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit monoclonal gfp antibody (Cell Signaling Technology Inc)


Structured Review

Rabbit Monoclonal Gfp Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal gfp antibody/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Bcl-x short-isoform is essential for maintaining homeostasis of multiple tissues"
Article Title: Bcl-x short-isoform is essential for maintaining homeostasis of multiple tissues
Journal: iScience
doi: 10.1016/j.isci.2023.106409

Figure Legend Snippet: Key resources table
Techniques Used: Subcloning, Recombinant, Transfection, Protease Inhibitor, Clone Assay, SYBR Green Assay, Plasmid Preparation, Purification, Gel Extraction, Isolation, Viability Assay, Knock-Out, Cell Culture, Mutagenesis, Software, Imaging, Modification
rabbit monoclonal gfp antibody (Cell Signaling Technology Inc)


Structured Review

Rabbit Monoclonal Gfp Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal gfp antibody/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Bcl-x short-isoform is essential for maintaining homeostasis of multiple tissues"
Article Title: Bcl-x short-isoform is essential for maintaining homeostasis of multiple tissues
Journal: iScience
doi: 10.1016/j.isci.2023.106409

Figure Legend Snippet: Key resources table
Techniques Used: Subcloning, Recombinant, Transfection, Protease Inhibitor, Clone Assay, SYBR Green Assay, Plasmid Preparation, Purification, Gel Extraction, Isolation, Viability Assay, Knock-Out, Cell Culture, Mutagenesis, Software, Imaging, Modification