rabbit monoclonal anti gapdh antibody  (Boster Bio)

Journal: PLoS ONE

Article Title: Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function

doi: 10.1371/journal.pone.0052404

Figure Lengend Snippet: ScFvs against Ig1–4 reduce, while scFvs against Fn1–3 enhance levels of phospho-src and downstream phospho-Erk1/2. Cultured SK-N-SH cells were incubated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 for 30 min in serum-free medium. ( A ) Levels of src and phospho-src of cells treated with L1/ecd (at 16.5 µM, right) or untreated (control, left), with lanes 1, 2 and 3 showing representative blots from three independent experiments with L1/ecd treated or untreated cells. ( B ) Src and phospho-src levels of cells treated with non-immune human IgG, L1/ecd or I27 (all at 16.5 µM) or left untreated (control). ( C ) Src and phospho-srclevels of cells treated with the indicated concentrations of scFv I4 or I6. ( D ) Src and phospho-src levels of cells treated with the indicated concentrations of scFv I13 or I27. ( E–F ) Comparison of levels of src and phospho-src ( E ) and Erk1/2 and phospho-Erk1/2 ( F ) in untreated cells (control) or cells treated with IgG, scFv I4, I6, I13 or I27 (all at 16.5 µM). Representative images from three independent experiments are shown in A–E . For the quantification shown in C–F , values of phospho-src ( C–E ) and phospho-Erk1/2 ( F ) were normalized to GAPDH as loading control. Mean values ± SEM from three independent experiments are shown. Asterisks denote significant differences from control. ** p

Article Snippet: Twenty five µg protein was subjected to 12% SDS-PAGE, transferred onto a PVDF membrane (Millipore, Temecula, CA, USA), and probed with rabbit anti-human src (Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-phospho-src (Santa Cruz Biotechnology), mouse anti-phospho-Erk1/2 (Beyotime), rabbit anti-human Erk1/2 (Beyotime), rabbit anti-human Bcl-2 (Biosis, Bei Jing, China), mouse anti-human Bax, mouse anti-human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (BOSTER, Wu Han, China).

Techniques: Cell Culture, Incubation, Positive Control, Negative Control

Journal: PLoS ONE

Article Title: Erythropoietin Inhibits Gluconeogenesis and Inflammation in the Liver and Improves Glucose Intolerance in High-Fat Diet-Fed Mice

doi: 10.1371/journal.pone.0053557

Figure Lengend Snippet: The effect of EPO on the expression of PEPCK and G6Pase in the liver. The relative levels of PEPCK and G6Pase in the livers of individual mice were determined by quantitative RT-PCR and Western blot assays. Data are representative images or expressed as the mean ± SEM of individual group (n = 8 per group) of mice from three separate experiments. ( A ) RT-PCR analysis of the relative levels of PEPCK and G6Pase mRNA transcripts to control GAPDH in the liver. (B) Western blot and quantitative analysis of the relative levels of PEPCK in the liver. ( C ) Western blot and quantitative analysis of the relative levels of G6Pase in the liver. # P

Article Snippet: The membranes were blocked with 5% fat-free milk and incubated with anti-IR, anti-IRS1 (Millipore, Billerica, USA), anti-JNK, anti-p-JNK (Thr183/Tyr185), anti-p-IRS1 (Ser307), anti-p-IR (Tyr1346), anti-Akt, anti-p-Akt (Ser473), anti-p38, anti-p-p38 (Thr180/Tyr182), anti-TLR4, anti-PEPCK, anti-G6Pase (Cell Signaling Technology, Danvers, USA), anti-GAPDH, anti-tubulin (Boster, Wuhan, China), respectively.

Techniques: Expressing, Mouse Assay, Quantitative RT-PCR, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Stem Cell Research & Therapy

Article Title: The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential

doi: 10.1186/s13287-018-0955-5

Figure Lengend Snippet: BMSCs pretreated with EMF exhibited stronger osteogenic differentiation potential. a RUNX2, ALP, OPN, and OCN mRNA levels of two groups analyzed by RT-PCR. GAPDH used as loading control for quantification ( n = 3). b Expression of OPN and RUNX2 proteins of both groups determined by western blot analysis. Relative densitometer values quantified by ImageJ software, GAPDH used as internal control ( n = 3). c Images of Alizarin Red S staining exhibited plaques of calcified extracellular matrix of both groups. Scale bar = 100 μm. d Semi-quantitative analysis of Alizarin Red S staining among both groups ( n = 6). Data shown as mean ± SD. * P

Article Snippet: Antibodies against GAPDH, RUNX2, secondary antibodies, and cell counting kit-8 (CCK-8) were provided by Boster (Wuhan, China).

Techniques: ALP Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Software, Staining

Journal: American Journal of Translational Research

Article Title: Inhibition of glioblastoma growth and invasion by 125I brachytherapy in rat glioma model

doi:

Figure Lengend Snippet: The implantation of 125 I seeds induced the mitochondria-dependent apoptotic pathway compared with the control. A: Western Blotting analysis of Bax, Bcl-2, pro-Caspase-3, Caspase-3, pro-Caspase-8, cleaved-Caspase-8, PARP, P53, β-Actin expression.

Article Snippet: After electrophoresis, proteins were electrotransferred to nitrocellulose membrane and immune complexes were formed by incubation of the proteins probed with primary antibodies, mouse anti-Bax, rabbit anti-Bcl-2, rabbit anti-Caspase-3, rabbit anti-Caspase-8 and rabbit anti-p53, rabbit anti-PARP, mouse anti-MMP-2, mouse anti-MMP-9 and mouse anti-Actin (Boster, Inc. China) for overnight at 4°C.

Techniques: Western Blot, Expressing