enolase 1  (Cell Signaling Technology Inc)

Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Source, application and concentration of antibodies.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Concentration Assay, Recombinant, Plasmid Preparation

Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Mass Spectrometry

Journal: Jornal Brasileiro de Pneumologia

Article Title: Diagnostic value of α-enolase expression and serum α-enolase autoantibody levels in lung cancer

doi: 10.1590/S1806-37562016000000241

Figure Lengend Snippet: Photomicrographs showing α-enolase (ENO1) expression in lung tissue samples (immunohistochemistry using the streptavidin-peroxidase staining method). Positive signs are visualized as yellow or brownish yellow granules. In A and B, adenocarcinoma tissue samples showing positive ENO1 expression (magnification, ×400 and ×100, respectively). In C and D, squamous cell carcinoma tissue samples showing positive ENO1 expression (magnification, ×400 and ×100, respectively). In E and F, pulmonary inflammatory pseudotumor tissue samples showing negative ENO1 expression (magnification, ×400 and ×100, respectively).

Article Snippet: The reagents used were rabbit anti-ENO1 monoclonal antibody (Abcam Biotechnology Co. Ltd., Cambridge, UK), an immunohistochemistry reagent kit (Maixin, Fuzhou, China) and an ENO1 antibody ELISA reagent kit (HuaAn, Hangzhou, China).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Jornal Brasileiro de Pneumologia

Article Title: Diagnostic value of α-enolase expression and serum α-enolase autoantibody levels in lung cancer

doi: 10.1590/S1806-37562016000000241

Figure Lengend Snippet: ROC curve showing the sensitivity and specificity of serum α-enolase antibody levels for the diagnosis of lung cancer.

Article Snippet: The reagents used were rabbit anti-ENO1 monoclonal antibody (Abcam Biotechnology Co. Ltd., Cambridge, UK), an immunohistochemistry reagent kit (Maixin, Fuzhou, China) and an ENO1 antibody ELISA reagent kit (HuaAn, Hangzhou, China).

Techniques:

Journal: Cells

Article Title: Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury

doi: 10.3390/cells11081329

Figure Lengend Snippet: Activation of PKA and glycolysis in Calr +/− kidney. The intensive mitochondrial damage results in an energy shortage. To overcome the energy crisis, the kidney cells in Calr +/− mice activate glycolysis. ( A ): Up-regulation of Slc5a1 a sodium/glucose cotransporter 1, which actively transports glucose into the cell, and PcK1 is also up-regulated to actively augment the glucose synthesis from lactate. ( B ): Enolase 1 is also up-regulated to favor the energy production from glucose. ( C ): PKA is significantly up-regulated and activated to accelerate glycolysis and to promote energy production, and the important glycolysis kinase PFK is significantly up-regulated in Calr +/− mouse kidneys. ( D ): Parallel to the activation of glycolysis, the enzymes involved in production of the alternative energy from phosphocreatine are also up-regulated in Calr +/− mouse kidney. data (*: p < 0.05) **: p < 0.01, ***: p < 0.001).

Article Snippet: Polyclonal rabbit anti-LC3 (NB-100-2220) was from Novus Biologicals (Centennial, CO, USA); rabbit monoclonal anti-Becn-1 (11//2016) was from Cell Signaling (Frankfurt am Main, Germany); rabbit monoclonal anti-Eno1 (ab155102), anti-Prdx6 (EPR3754), anti-iNos1 (ab178945), anti-Ncx1 (ab177952), anti-Pvalb (ab181086), anti-Phb (ab75766), anti-p65 (ab32536), anti-Trpv5 (ab137028), anti-Vdac1 (ab154856), anti-Col-1 (ab270994), rabbit polyclonal anti-Bcl-2 (ab196495), goat anti-Calr (ab2907), anti-Pdia3 (ab228789), anti-pEif2-alpha (ab131505), mouse monoclonal anti-Calm1 (ab2860), anti-Canx (ab112995), and anti-IkB (ab12134) antibodies were from Abcam (Berlin, Germany).

Techniques: Activation Assay

Journal: Nature neuroscience

Article Title: A glycolytic shift in Schwann cells supports injured axons

doi: 10.1038/s41593-020-0689-4

Figure Lengend Snippet: a-i, Representative immunofluorescence for the indicated metabolic components on longitudinal frozen sections from uninjured control nerve segments and axotomized distal sciatic nerve stumps at the shown post-injury times. HK2: Hexokinase 2 (a). GPI: Glucose-6-phosphate isomerase (b). ALDA: Aldolase A (c). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase (d). PGK1: Phosphoglycerate kinase 1 (e). PGAM1: Phosphoglycerate mutase 1 (f). ENO1: Enolase 1 (g). PKM1: Pyruvate kinase M1 (h). LDHB: Lactate dehydrogenase B (i). Arrows depict colocalization in SCs. Scale bars: 50μm. The experiments for each component were reproduced three times independently with similar results.

Article Snippet: Following primary antibodies were used for nerve and teased fiber immunofluorescence: Neurofilament 200 (1:500, Sigma, N4142), Hexokinase I C35C4 (1:200, Cell Signaling, 2024), Hexokinase II C64G5 (1:200, Cell Signaling, 2867), GPI (1:200, Proteintech, 15171-1-AP), PFKM (1:200, Proteintech, 55028-1-AP), Aldolase A D73H4 (1:200, Cell Signaling, 8060), GAPDH (1:500, Sigma, G9545), PGK1 (1:150, Proteintech, 17811-1-AP), PGAM1 D3J9T (1:50, Cell Signaling, 12098), Enolase-1 D2S1A (1:200, Cell Signaling, 13410), PKM1 D30G6 (1:400, Cell Signaling, 7067), PKM2 D78A4 (1:200, Cell Signaling, 4053), PFKFB3 D7H4Q (1:200, Cell Signaling, 13123), LDHA/LDHC C28H7 (1:200, Cell Signaling, 3558), LDHB (1:200, Proteintech, 14824-1-AP), Glut1 D3J3A (1:200, Cell Signaling, 12939), PDHK1 C47H1 (1:100, Cell Signaling, 3820), Pyruvate Dehydrogenase C54G1 (1:100, Cell Signaling, 3205), CS (1:150, Proteintech, 16131-1-AP), IDH3A (1:100, Proteintech, 15909-1-AP), OGDH (1:50, Proteintech, 15212-1-AP), MCT1 M-45 (1:200, Santa Cruz Biotechnology, sc-50325), MCT4 H-90 (1:250, Santa Cruz Biotechnology, sc-50329), Phospho-S6 Ribosomal Protein (Ser240/244) D68F8 (1:800, Cell Signaling, 5364), Phospho-Akt (Ser473) D9E (1:100, Cell Signaling, 4060), HIF-1 alpha (1:200, Novus Biologicals, NB100-479), c-Myc D84C12 (1:800, Cell Signaling, 5605), c-Myc/N-Myc D3N8F (1:800, Cell Signaling, 13987), Phospho-AMPKα (Thr172) 40H9 (1:100, Cell Signaling, 2535), S100 4C4.9 (1:200, Thermo Fisher Scientific, MA5-12966), P0 (1:1000, Aves Labs, PZO), TUJ1/TUBB3 (1:500, BioLegend, 801202).

Techniques: Expressing, Immunofluorescence

Journal: iScience

Article Title: Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling

doi: 10.1016/j.isci.2021.103607

Figure Lengend Snippet: Proteo-transcriptomic integration confirms HIF signaling and glycolysis as unique features of the male EC phenotype Proteo-transcriptomic analysis in HC (n = 9 ), EC (n = 9 ), and VP (n = 9 ). (A) Upset plot describing the number of features detected by each of the sparse partial least squares models. (B) Schematic representation of the derivation of EC-specific proteins. (C) PCA plot representing sample clustering with respect to EC-specific proteins. The first two principal components capturing maximum of variance were used. Data ellipses were drawn at the level of 0.9. (D) Heatmap visualizing quantile normalized and Z-scaled expression pattern of EC-specific proteins. Column annotation represents the cohorts and the different groups used for pairwise comparison. Proteins are hierarchically clustered based on Euclidean distance. (E) Functional analysis results of proteins belonging to cluster 1 in (D). Size of the bubble and the color gradient are relative to number of features in each pathway and adjusted p value of the enrichment test, respectively. (F) Functional analysis results of the proteins belonging to cluster 2 in (D). The bubble size and color gradient are relative to number of features in each pathway and adjusted p value of the enrichment test, respectively. (G) Schematic representation of the proteins belonging to pathways in (F). Circular nodes in red represents upregulated proteins in EC, in relation to HC, and gray color denotes non-significant expression levels. (H) Western blot analysis of HIF target genes identified in (G) for male HC (n = 9 ), and EC (n = 9 ). (I) Violin plots representing relative protein quantification of HIF-1α, ENO1, and ENO3 normalized to β-Actin from (H) using unpaired t test (significance level, p < 0.05) represented with violin plot with mean. See also Figure S2 .

Article Snippet: Rabbit monoclonal anti-ENO1 [EPR10863(B)] , Abcam , Cat#ab155102.

Techniques: Expressing, Functional Assay, Western Blot

Journal: iScience

Article Title: Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling

doi: 10.1016/j.isci.2021.103607

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-ENO1 [EPR10863(B)] , Abcam , Cat#ab155102.

Techniques: Software