rabbit monoclonal eno1  (Abcam)

Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Source, application and concentration of antibodies.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Concentration Assay, Recombinant, Plasmid Preparation

Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Mass Spectrometry

Journal: Jornal Brasileiro de Pneumologia

Article Title: Diagnostic value of α-enolase expression and serum α-enolase autoantibody levels in lung cancer

doi: 10.1590/S1806-37562016000000241

Figure Lengend Snippet: Photomicrographs showing α-enolase (ENO1) expression in lung tissue samples (immunohistochemistry using the streptavidin-peroxidase staining method). Positive signs are visualized as yellow or brownish yellow granules. In A and B, adenocarcinoma tissue samples showing positive ENO1 expression (magnification, ×400 and ×100, respectively). In C and D, squamous cell carcinoma tissue samples showing positive ENO1 expression (magnification, ×400 and ×100, respectively). In E and F, pulmonary inflammatory pseudotumor tissue samples showing negative ENO1 expression (magnification, ×400 and ×100, respectively).

Article Snippet: The reagents used were rabbit anti-ENO1 monoclonal antibody (Abcam Biotechnology Co. Ltd., Cambridge, UK), an immunohistochemistry reagent kit (Maixin, Fuzhou, China) and an ENO1 antibody ELISA reagent kit (HuaAn, Hangzhou, China).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Jornal Brasileiro de Pneumologia

Article Title: Diagnostic value of α-enolase expression and serum α-enolase autoantibody levels in lung cancer

doi: 10.1590/S1806-37562016000000241

Figure Lengend Snippet: ROC curve showing the sensitivity and specificity of serum α-enolase antibody levels for the diagnosis of lung cancer.

Article Snippet: The reagents used were rabbit anti-ENO1 monoclonal antibody (Abcam Biotechnology Co. Ltd., Cambridge, UK), an immunohistochemistry reagent kit (Maixin, Fuzhou, China) and an ENO1 antibody ELISA reagent kit (HuaAn, Hangzhou, China).

Techniques:

Journal: Cells

Article Title: Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury

doi: 10.3390/cells11081329

Figure Lengend Snippet: Activation of PKA and glycolysis in Calr +/− kidney. The intensive mitochondrial damage results in an energy shortage. To overcome the energy crisis, the kidney cells in Calr +/− mice activate glycolysis. ( A ): Up-regulation of Slc5a1 a sodium/glucose cotransporter 1, which actively transports glucose into the cell, and PcK1 is also up-regulated to actively augment the glucose synthesis from lactate. ( B ): Enolase 1 is also up-regulated to favor the energy production from glucose. ( C ): PKA is significantly up-regulated and activated to accelerate glycolysis and to promote energy production, and the important glycolysis kinase PFK is significantly up-regulated in Calr +/− mouse kidneys. ( D ): Parallel to the activation of glycolysis, the enzymes involved in production of the alternative energy from phosphocreatine are also up-regulated in Calr +/− mouse kidney. data (*: p < 0.05) **: p < 0.01, ***: p < 0.001).

Article Snippet: Polyclonal rabbit anti-LC3 (NB-100-2220) was from Novus Biologicals (Centennial, CO, USA); rabbit monoclonal anti-Becn-1 (11//2016) was from Cell Signaling (Frankfurt am Main, Germany); rabbit monoclonal anti-Eno1 (ab155102), anti-Prdx6 (EPR3754), anti-iNos1 (ab178945), anti-Ncx1 (ab177952), anti-Pvalb (ab181086), anti-Phb (ab75766), anti-p65 (ab32536), anti-Trpv5 (ab137028), anti-Vdac1 (ab154856), anti-Col-1 (ab270994), rabbit polyclonal anti-Bcl-2 (ab196495), goat anti-Calr (ab2907), anti-Pdia3 (ab228789), anti-pEif2-alpha (ab131505), mouse monoclonal anti-Calm1 (ab2860), anti-Canx (ab112995), and anti-IkB (ab12134) antibodies were from Abcam (Berlin, Germany).

Techniques: Activation Assay

Journal: Nature neuroscience

Article Title: A glycolytic shift in Schwann cells supports injured axons

doi: 10.1038/s41593-020-0689-4

Figure Lengend Snippet: a-i, Representative immunofluorescence for the indicated metabolic components on longitudinal frozen sections from uninjured control nerve segments and axotomized distal sciatic nerve stumps at the shown post-injury times. HK2: Hexokinase 2 (a). GPI: Glucose-6-phosphate isomerase (b). ALDA: Aldolase A (c). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase (d). PGK1: Phosphoglycerate kinase 1 (e). PGAM1: Phosphoglycerate mutase 1 (f). ENO1: Enolase 1 (g). PKM1: Pyruvate kinase M1 (h). LDHB: Lactate dehydrogenase B (i). Arrows depict colocalization in SCs. Scale bars: 50μm. The experiments for each component were reproduced three times independently with similar results.

Article Snippet: Following primary antibodies were used for nerve and teased fiber immunofluorescence: Neurofilament 200 (1:500, Sigma, N4142), Hexokinase I C35C4 (1:200, Cell Signaling, 2024), Hexokinase II C64G5 (1:200, Cell Signaling, 2867), GPI (1:200, Proteintech, 15171-1-AP), PFKM (1:200, Proteintech, 55028-1-AP), Aldolase A D73H4 (1:200, Cell Signaling, 8060), GAPDH (1:500, Sigma, G9545), PGK1 (1:150, Proteintech, 17811-1-AP), PGAM1 D3J9T (1:50, Cell Signaling, 12098), Enolase-1 D2S1A (1:200, Cell Signaling, 13410), PKM1 D30G6 (1:400, Cell Signaling, 7067), PKM2 D78A4 (1:200, Cell Signaling, 4053), PFKFB3 D7H4Q (1:200, Cell Signaling, 13123), LDHA/LDHC C28H7 (1:200, Cell Signaling, 3558), LDHB (1:200, Proteintech, 14824-1-AP), Glut1 D3J3A (1:200, Cell Signaling, 12939), PDHK1 C47H1 (1:100, Cell Signaling, 3820), Pyruvate Dehydrogenase C54G1 (1:100, Cell Signaling, 3205), CS (1:150, Proteintech, 16131-1-AP), IDH3A (1:100, Proteintech, 15909-1-AP), OGDH (1:50, Proteintech, 15212-1-AP), MCT1 M-45 (1:200, Santa Cruz Biotechnology, sc-50325), MCT4 H-90 (1:250, Santa Cruz Biotechnology, sc-50329), Phospho-S6 Ribosomal Protein (Ser240/244) D68F8 (1:800, Cell Signaling, 5364), Phospho-Akt (Ser473) D9E (1:100, Cell Signaling, 4060), HIF-1 alpha (1:200, Novus Biologicals, NB100-479), c-Myc D84C12 (1:800, Cell Signaling, 5605), c-Myc/N-Myc D3N8F (1:800, Cell Signaling, 13987), Phospho-AMPKα (Thr172) 40H9 (1:100, Cell Signaling, 2535), S100 4C4.9 (1:200, Thermo Fisher Scientific, MA5-12966), P0 (1:1000, Aves Labs, PZO), TUJ1/TUBB3 (1:500, BioLegend, 801202).

Techniques: Expressing, Immunofluorescence