Journal: iScience

Article Title: Bromodomain protein BRD8 regulates cell cycle progression in colorectal cancer cells through a TIP60-independent regulation of the pre-RC complex

doi: 10.1016/j.isci.2023.106563

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Article Snippet: Rabbit monoclonal anti-c-Fos , Cell Signaling Technology , Cat# 2250; RRID:AB_2247211.

Techniques: Recombinant, Plasmid Preparation, Cell Cycle Assay, CCK-8 Assay, Flow Cytometry, Viability Assay, Expressing, Clone Assay, Mutagenesis, Software

Journal: Science Advances

Article Title: GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency

doi: 10.1126/sciadv.ade5397

Figure Lengend Snippet: ( A ) AAV microinjection into the ACC. ( B ) The experimental design for hM4Di-DREADD validation in vGat-Cre mice. ( C ) Immunohistochemical staining showing Fos and hM4Di-mCherry expression 90 min after the injection of either CNO or vehicle in vGat-Cre mice microinjected with AAV-Syn-DIO-hM4Di-mCherry. Scale bars, 200 μm. ( D ) Fos quantification in the ACC (unpaired t test, t 9 = 3.967, P < 0.01). n = 5 to 6 per group. ( E ) Immunohistochemical staining showing Fos and CaMKIIα expression after the injection of either CNO or vehicle in vGat-Cre mice injected with AAV-Syn-DIO-hM4Di-mCherry. Scale bars, 100 μm. ( F ) Fos quantification in CaMKIIα-positive neurons (E) (unpaired t test, t 8 = 4.714, P < 0.01). n = 5 to 6 per group. ( G ) The experimental design for behavioral assays. ( H and I ) Social interaction (SI) ratio of hM4Di mice administered vehicle (H) (paired t test, SIT-1 versus SIT-2, t 8, = 0.4389, P > 0.05; SIT-1 versus SIT-3, t 8, = 1.38, P > 0.05) and CNO (I) in the SIT (paired t test, SIT-1 versus SIT-2, t 9, = 2.509, P < 0.05; SIT-1 versus SIT-3, t 9, = 2.64, P < 0.05). n = 9 to 10 per group. * P < 0.05 and ** P < 0.01. Bar graphs show the means ± SEM.

Article Snippet: Antibodies against Fos (1:500 or 1:1000; Cell Signaling, #2250), CaMKIIα (1:500; Abcam, ab22609), NeuN (1:1000; Millipore, MAB377), and hemagglutinin (HA; 1:1000, Merck, #11867423001) were used.

Techniques: Immunohistochemical staining, Staining, Expressing, Injection