rabbit monoclonal anti acetylated lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti acetylated lysine
    Rabbit Monoclonal Anti Acetylated Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti mouse monoclonal acetyl lysine antibody  (Danaher Inc)


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    Danaher Inc rabbit anti mouse monoclonal acetyl lysine antibody
    Rabbit Anti Mouse Monoclonal Acetyl Lysine Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti mouse monoclonal acetyl lysine antibody  (Abcam)

     
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    Abcam rabbit anti mouse monoclonal acetyl lysine antibody
    Rabbit Anti Mouse Monoclonal Acetyl Lysine Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit monoclonal anti acetylated lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti acetylated lysine
    Rabbit Monoclonal Anti Acetylated Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti acetylated lysine/product/Cell Signaling Technology Inc
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    anti acetyl lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti acetyl lysine
    Anti Acetyl Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti acetyl lysine/product/Cell Signaling Technology Inc
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    rabbit monoclonal anti acetyl lysine  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti acetyl lysine

    Rabbit Monoclonal Anti Acetyl Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti acetyl lysine/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
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    rabbit monoclonal anti acetyl lysine - by Bioz Stars, 2024-07
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    1) Product Images from "Histone malonylation is regulated by SIRT5 and KAT2A"

    Article Title: Histone malonylation is regulated by SIRT5 and KAT2A

    Journal: iScience

    doi: 10.1016/j.isci.2023.106193


    Figure Legend Snippet:

    Techniques Used: Recombinant, Plasmid Preparation, Software

    acetylated lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc acetylated lysine
    Acetylated Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit monoclonal anti acetyl lysine antibody anti ack  (ImmuneChem)

     
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    ImmuneChem rabbit monoclonal anti acetyl lysine antibody anti ack
    Rabbit Monoclonal Anti Acetyl Lysine Antibody Anti Ack, supplied by ImmuneChem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti acetylated histone 3 lysine 27 h3k27ac antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti acetylated histone 3 lysine 27 h3k27ac antibody
    (A) Reduced cytosolic citrate and acetyl-CoA (Ac-CoA) levels in Mir140UGCG mice. Ac-CoA and citrate levels were determined in primary rib chondrocytes from 10-day-old mice. (B) Immunofluorescence of indicated acetylated histones in the spheno-occipital growth plate. Dotted boxes indicate the resting zone. Insets are magnified views of the resting zone. MIr1430UGCG mice show reduced histone acetylation. (C) Immunoblot analysis for H3K27 acetylation. (D) Immunofluorescence assessment of <t>H3K27</t> <t>acetylation</t> in the tibial growth plate of control and Ldha cKO mice. Insets are magnified views of dotted boxes. (E) Quantification of H3K27 acetylation <t>(H3K27Ac)</t> in D . H3K27Ac-positive cells were counted in the resting zone and proliferating zone and normalized to the total number of cells per area. Ldha cKO show reduced numbers of chondrocytes positive for H3K27Ac. (F) Immunoblot quantification of acetylation of indicated histones in primary rib chondrocytes. Ldha cKO shows reduced histone acetylation.
    Anti Acetylated Histone 3 Lysine 27 H3k27ac Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti acetylated histone 3 lysine 27 h3k27ac antibody/product/Cell Signaling Technology Inc
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    1) Product Images from "Reduced glycolysis links resting zone chondrocyte proliferation in the growth plate"

    Article Title: Reduced glycolysis links resting zone chondrocyte proliferation in the growth plate

    Journal: bioRxiv

    doi: 10.1101/2023.01.18.524550

    (A) Reduced cytosolic citrate and acetyl-CoA (Ac-CoA) levels in Mir140UGCG mice. Ac-CoA and citrate levels were determined in primary rib chondrocytes from 10-day-old mice. (B) Immunofluorescence of indicated acetylated histones in the spheno-occipital growth plate. Dotted boxes indicate the resting zone. Insets are magnified views of the resting zone. MIr1430UGCG mice show reduced histone acetylation. (C) Immunoblot analysis for H3K27 acetylation. (D) Immunofluorescence assessment of H3K27 acetylation in the tibial growth plate of control and Ldha cKO mice. Insets are magnified views of dotted boxes. (E) Quantification of H3K27 acetylation (H3K27Ac) in D . H3K27Ac-positive cells were counted in the resting zone and proliferating zone and normalized to the total number of cells per area. Ldha cKO show reduced numbers of chondrocytes positive for H3K27Ac. (F) Immunoblot quantification of acetylation of indicated histones in primary rib chondrocytes. Ldha cKO shows reduced histone acetylation.
    Figure Legend Snippet: (A) Reduced cytosolic citrate and acetyl-CoA (Ac-CoA) levels in Mir140UGCG mice. Ac-CoA and citrate levels were determined in primary rib chondrocytes from 10-day-old mice. (B) Immunofluorescence of indicated acetylated histones in the spheno-occipital growth plate. Dotted boxes indicate the resting zone. Insets are magnified views of the resting zone. MIr1430UGCG mice show reduced histone acetylation. (C) Immunoblot analysis for H3K27 acetylation. (D) Immunofluorescence assessment of H3K27 acetylation in the tibial growth plate of control and Ldha cKO mice. Insets are magnified views of dotted boxes. (E) Quantification of H3K27 acetylation (H3K27Ac) in D . H3K27Ac-positive cells were counted in the resting zone and proliferating zone and normalized to the total number of cells per area. Ldha cKO show reduced numbers of chondrocytes positive for H3K27Ac. (F) Immunoblot quantification of acetylation of indicated histones in primary rib chondrocytes. Ldha cKO shows reduced histone acetylation.

    Techniques Used: Immunofluorescence, Western Blot

    (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.
    Figure Legend Snippet: (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.

    Techniques Used: Staining, Western Blot

    anti acetylate histone 3 lysine 9 h3k9ac antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti acetylate histone 3 lysine 9 h3k9ac antibody
    (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced <t>histone</t> <t>3</t> acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.
    Anti Acetylate Histone 3 Lysine 9 H3k9ac Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti acetylate histone 3 lysine 9 h3k9ac antibody/product/Cell Signaling Technology Inc
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    Images

    1) Product Images from "Reduced glycolysis links resting zone chondrocyte proliferation in the growth plate"

    Article Title: Reduced glycolysis links resting zone chondrocyte proliferation in the growth plate

    Journal: bioRxiv

    doi: 10.1101/2023.01.18.524550

    (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.
    Figure Legend Snippet: (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.

    Techniques Used: Staining, Western Blot

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    Cell Signaling Technology Inc rabbit monoclonal anti acetylated lysine
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    ImmuneChem rabbit monoclonal anti acetyl lysine antibody anti ack

    Rabbit Monoclonal Anti Acetyl Lysine Antibody Anti Ack, supplied by ImmuneChem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti acetylated histone 3 lysine 27 h3k27ac antibody
    (A) Reduced cytosolic citrate and acetyl-CoA (Ac-CoA) levels in Mir140UGCG mice. Ac-CoA and citrate levels were determined in primary rib chondrocytes from 10-day-old mice. (B) Immunofluorescence of indicated acetylated histones in the spheno-occipital growth plate. Dotted boxes indicate the resting zone. Insets are magnified views of the resting zone. MIr1430UGCG mice show reduced histone acetylation. (C) Immunoblot analysis for H3K27 acetylation. (D) Immunofluorescence assessment of <t>H3K27</t> <t>acetylation</t> in the tibial growth plate of control and Ldha cKO mice. Insets are magnified views of dotted boxes. (E) Quantification of H3K27 acetylation <t>(H3K27Ac)</t> in D . H3K27Ac-positive cells were counted in the resting zone and proliferating zone and normalized to the total number of cells per area. Ldha cKO show reduced numbers of chondrocytes positive for H3K27Ac. (F) Immunoblot quantification of acetylation of indicated histones in primary rib chondrocytes. Ldha cKO shows reduced histone acetylation.
    Anti Acetylated Histone 3 Lysine 27 H3k27ac Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti acetylate histone 3 lysine 9 h3k9ac antibody
    (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced <t>histone</t> <t>3</t> acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.
    Anti Acetylate Histone 3 Lysine 9 H3k9ac Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Journal: iScience

    Article Title: Histone malonylation is regulated by SIRT5 and KAT2A

    doi: 10.1016/j.isci.2023.106193

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal anti-acetyl-lysine , Cell Signaling Technology , Cat. #9814, RRID: AB_10544700.

    Techniques: Recombinant, Plasmid Preparation, Software

    (A) Reduced cytosolic citrate and acetyl-CoA (Ac-CoA) levels in Mir140UGCG mice. Ac-CoA and citrate levels were determined in primary rib chondrocytes from 10-day-old mice. (B) Immunofluorescence of indicated acetylated histones in the spheno-occipital growth plate. Dotted boxes indicate the resting zone. Insets are magnified views of the resting zone. MIr1430UGCG mice show reduced histone acetylation. (C) Immunoblot analysis for H3K27 acetylation. (D) Immunofluorescence assessment of H3K27 acetylation in the tibial growth plate of control and Ldha cKO mice. Insets are magnified views of dotted boxes. (E) Quantification of H3K27 acetylation (H3K27Ac) in D . H3K27Ac-positive cells were counted in the resting zone and proliferating zone and normalized to the total number of cells per area. Ldha cKO show reduced numbers of chondrocytes positive for H3K27Ac. (F) Immunoblot quantification of acetylation of indicated histones in primary rib chondrocytes. Ldha cKO shows reduced histone acetylation.

    Journal: bioRxiv

    Article Title: Reduced glycolysis links resting zone chondrocyte proliferation in the growth plate

    doi: 10.1101/2023.01.18.524550

    Figure Lengend Snippet: (A) Reduced cytosolic citrate and acetyl-CoA (Ac-CoA) levels in Mir140UGCG mice. Ac-CoA and citrate levels were determined in primary rib chondrocytes from 10-day-old mice. (B) Immunofluorescence of indicated acetylated histones in the spheno-occipital growth plate. Dotted boxes indicate the resting zone. Insets are magnified views of the resting zone. MIr1430UGCG mice show reduced histone acetylation. (C) Immunoblot analysis for H3K27 acetylation. (D) Immunofluorescence assessment of H3K27 acetylation in the tibial growth plate of control and Ldha cKO mice. Insets are magnified views of dotted boxes. (E) Quantification of H3K27 acetylation (H3K27Ac) in D . H3K27Ac-positive cells were counted in the resting zone and proliferating zone and normalized to the total number of cells per area. Ldha cKO show reduced numbers of chondrocytes positive for H3K27Ac. (F) Immunoblot quantification of acetylation of indicated histones in primary rib chondrocytes. Ldha cKO shows reduced histone acetylation.

    Article Snippet: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac) antibody (#9649, Cell Signaling Technology).

    Techniques: Immunofluorescence, Western Blot

    (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.

    Journal: bioRxiv

    Article Title: Reduced glycolysis links resting zone chondrocyte proliferation in the growth plate

    doi: 10.1101/2023.01.18.524550

    Figure Lengend Snippet: (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.

    Article Snippet: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac) antibody (#9649, Cell Signaling Technology).

    Techniques: Staining, Western Blot

    (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.

    Journal: bioRxiv

    Article Title: Reduced glycolysis links resting zone chondrocyte proliferation in the growth plate

    doi: 10.1101/2023.01.18.524550

    Figure Lengend Snippet: (A) Acly cKO mice show shortening and bowing of the tibia ( Top) . Mice are perinatally lethal. H/E-stained sections of the tibia at P 0.5. The bottom panels show magnified views of proliferating chondrocytes in the boxed areas. The flat morphology of the columnar chondrocytes is lost in cKO mice. (B) Expansion of the resting zone chondrocytes in the spheno-occipital growth plate in E18.5 Acly cKO mice. Left , H/E-stained ( Top ) and EdU-stained ( Bottom ) sections. Boxed areas indicate the resting zone. Right , The number of resting chondrocytes is increased in the Acly cKO growth plate. (C) Reduced histone 3 acetylation in primary rib chondrocytes from E18.5 Acly cKO mouse embryos. Whole-cell lysates from an overnight culture of primary rib chondrocytes were subjected to immunoblot analysis. Acly cKO chondrocytes show reduced acetylation of H3K27 and H3K9. (D). Differential phosphorylation of AMPK between Acly cKO and Ldh compound cKO chondrocytes. The level of p-AMPK is unchanged in Acly cKO, whereas it is upregulated in Ldh compound cKO cells, suggesting ATP deficiency in Ldh compound cKO, but not in Acly cKO chondrocytes.

    Article Snippet: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac) antibody (#9649, Cell Signaling Technology).

    Techniques: Staining, Western Blot