Journal: The Analyst

Article Title: A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1

doi: 10.1039/d0an00174k

Figure Lengend Snippet: IDH1 catalyzes the conversion of isocitrate to αKG.

Article Snippet: The membrane was then blocked using Odyssey® TBS blocking buffer (LI-COR, Lincoln, NE) at room temperature for 1 hour and incubated overnight with rabbit IgG anti-IDH1 (Cell Signaling Technology, Danvers, MA) at a 1 : 1000 dilution and mouse IgG1 anti-HSP70 (BD Biosciences, Franklin Lanes, NJ) at a 1 : 2000 dilution at 4 °C.

Techniques:

Journal: The Analyst

Article Title: A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1

doi: 10.1039/d0an00174k

Figure Lengend Snippet: (a) Scheme for the preparation and analysis of recombinant IDH1 samples. (b) αKG concentrations measured in the 384-sample biochemical screen (7 samples did not produce either 12C or 13C αKG capture).

Article Snippet: The membrane was then blocked using Odyssey® TBS blocking buffer (LI-COR, Lincoln, NE) at room temperature for 1 hour and incubated overnight with rabbit IgG anti-IDH1 (Cell Signaling Technology, Danvers, MA) at a 1 : 1000 dilution and mouse IgG1 anti-HSP70 (BD Biosciences, Franklin Lanes, NJ) at a 1 : 2000 dilution at 4 °C.

Techniques: Recombinant

Journal: The Analyst

Article Title: A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1

doi: 10.1039/d0an00174k

Figure Lengend Snippet: IDH1 expression level as determined with western blot and αKG concentration as measured with SAMDI-MS for untreated cells and cells treated with transfection agent, control sequence siRNA and IDH1-targeting siRNA. All data are expressed as mean ± standard deviation with n = 2 experimental replicates.

Article Snippet: The membrane was then blocked using Odyssey® TBS blocking buffer (LI-COR, Lincoln, NE) at room temperature for 1 hour and incubated overnight with rabbit IgG anti-IDH1 (Cell Signaling Technology, Danvers, MA) at a 1 : 1000 dilution and mouse IgG1 anti-HSP70 (BD Biosciences, Franklin Lanes, NJ) at a 1 : 2000 dilution at 4 °C.

Techniques: Expressing, Western Blot, Concentration Assay, Transfection, Sequencing, Standard Deviation