rabbit anti endothelial nitric oxide synthase enos  (Bioss)


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    Bioss rabbit anti endothelial nitric oxide synthase enos
    Rabbit Anti Endothelial Nitric Oxide Synthase Enos, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti endothelial nitric oxide synthase enos/product/Bioss
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti endothelial nitric oxide synthase enos - by Bioz Stars, 2024-07
    93/100 stars

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    rabbit anti human eno1 igg antibody  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
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    Danaher Inc rabbit anti human eno1 igg antibody
    Reagents and tools table
    Rabbit Anti Human Eno1 Igg Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human eno1 igg antibody/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human eno1 igg antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Single-cell and spatial transcriptomics reveal a high glycolysis B cell and tumor-associated macrophages cluster correlated with poor prognosis and exhausted immune microenvironment in diffuse large B-cell lymphoma"

    Article Title: Single-cell and spatial transcriptomics reveal a high glycolysis B cell and tumor-associated macrophages cluster correlated with poor prognosis and exhausted immune microenvironment in diffuse large B-cell lymphoma

    Journal: Biomarker Research

    doi: 10.1186/s40364-024-00605-w

    Reagents and tools table
    Figure Legend Snippet: Reagents and tools table

    Techniques Used: RNA Sequencing Assay, Sequencing, Expressing, Formalin-fixed Paraffin-Embedded, Immunohistochemistry, Microscopy, Immunofluorescence, Software

    Identifcation of glycolysis / gluconeogenesis maker genes in high malignant B cells. A Volcano plot of differential genes among the benign B cells, low malignant B cells, and high malignant B cells. B. Functional analysis of highly expressed genes in high malignant B cells by Metascape. C. Identification of eight commonly genes overlapped across three groups and glycolysis hallmark genes. D. Module trait correlation showed the relationships between modules, CNV score, and Glycolysis score. E. Network visualization of 10 modules of high maligant B cells.( The modules highlighted in red and underlined are modules associated with CNV score and Glycolysis score. ) F. The first 25 eigengenes of each module. G. Trajectory of different malignant B subclusters predicted by monocle. H. Genes expression level in single spot ordered along the pseudotime for MKI67 and seven glycolysis / gluconeogenesis gene markers ( STMN1, ENO1, LDHA, TPI1, CDK1, PKM , and PPIA ). ( Abbreviation : HMB: high malignant B cells; CNV: copy number variation; UMAP: uniform manifold approximation and projection. *** p < 0.001.)
    Figure Legend Snippet: Identifcation of glycolysis / gluconeogenesis maker genes in high malignant B cells. A Volcano plot of differential genes among the benign B cells, low malignant B cells, and high malignant B cells. B. Functional analysis of highly expressed genes in high malignant B cells by Metascape. C. Identification of eight commonly genes overlapped across three groups and glycolysis hallmark genes. D. Module trait correlation showed the relationships between modules, CNV score, and Glycolysis score. E. Network visualization of 10 modules of high maligant B cells.( The modules highlighted in red and underlined are modules associated with CNV score and Glycolysis score. ) F. The first 25 eigengenes of each module. G. Trajectory of different malignant B subclusters predicted by monocle. H. Genes expression level in single spot ordered along the pseudotime for MKI67 and seven glycolysis / gluconeogenesis gene markers ( STMN1, ENO1, LDHA, TPI1, CDK1, PKM , and PPIA ). ( Abbreviation : HMB: high malignant B cells; CNV: copy number variation; UMAP: uniform manifold approximation and projection. *** p < 0.001.)

    Techniques Used: Functional Assay, Expressing

    Prognostic value of four glycolysis / gluconeogenesis (STMN1, ENO1, CDK1, PKM, and PPIA) proteins in IHC cohort ( n = 34, 100X) and IFN_TAMs (CD68 + CXCL10 + PD-L1 + ) in mIF cohort ( n = 20, 10X). A-B. Kaplan–Meier curves of OS and PFS according to STMN1, CDK1, ENO1 and PKM proteins expression. C. Representative IHC staining of STMN1, CDK1, ENO1 and PKM in patient 1 (PFS = 7 months, OS = 9 months) and patient 2 (PFS = 135 months, OS = 135 months). D. Kaplan-Meier analysis for PFS based on IFN_TAMs intensity. E. Comparison of IFN_TAMs intensity in relapse and non_relapse groups. F. Correlation of IFN_TAMs’ intensity, CD8 + T cells’ intensity, and TGFβ1 intensity. G. Representative mIF staining of IFN_TAMs and CD8 + T cells in patient #1 (PFS = 2.7 months) and patient #2 (PFS = 90 months). ( Abbreviation : IHC: immunohistochemistry; IFN_TAMs: interferon-primed tumor-associated macrophages; mIF: multiple immunofluorescence; OS: overall survival; PFS: progression -free survival, Relapse: relapsed patients, patients without EFS24; Non_Relapse: non-relapsed patients, patients with EFS24. Mann-Whitney test was performed between groups.)
    Figure Legend Snippet: Prognostic value of four glycolysis / gluconeogenesis (STMN1, ENO1, CDK1, PKM, and PPIA) proteins in IHC cohort ( n = 34, 100X) and IFN_TAMs (CD68 + CXCL10 + PD-L1 + ) in mIF cohort ( n = 20, 10X). A-B. Kaplan–Meier curves of OS and PFS according to STMN1, CDK1, ENO1 and PKM proteins expression. C. Representative IHC staining of STMN1, CDK1, ENO1 and PKM in patient 1 (PFS = 7 months, OS = 9 months) and patient 2 (PFS = 135 months, OS = 135 months). D. Kaplan-Meier analysis for PFS based on IFN_TAMs intensity. E. Comparison of IFN_TAMs intensity in relapse and non_relapse groups. F. Correlation of IFN_TAMs’ intensity, CD8 + T cells’ intensity, and TGFβ1 intensity. G. Representative mIF staining of IFN_TAMs and CD8 + T cells in patient #1 (PFS = 2.7 months) and patient #2 (PFS = 90 months). ( Abbreviation : IHC: immunohistochemistry; IFN_TAMs: interferon-primed tumor-associated macrophages; mIF: multiple immunofluorescence; OS: overall survival; PFS: progression -free survival, Relapse: relapsed patients, patients without EFS24; Non_Relapse: non-relapsed patients, patients with EFS24. Mann-Whitney test was performed between groups.)

    Techniques Used: Expressing, Immunohistochemistry, Comparison, Staining, Immunofluorescence, MANN-WHITNEY

    rabbit anti eno1  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
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    Danaher Inc rabbit anti eno1
    HUVECs’ genetic and metabolic alterations to GExos treatment in HG culture. (A1 to A2) Enrichment plots from GSEAs of gene sets for the “Positive regulation of glycolysis signaling”. Expression levels and quantitative analysis of (B1 to B4) PFKM, PGK1, and <t>ENO1,</t> and (C1 to C4) PGLS, ACACA, and PDHA1. (D) Schematic illustration of the major genes and metabolic signaling pathways relevant to angiogenesis, regulated by GExos. (E) Volcano plots of differentially expressed metabolites identified at q < 0.05. (F) KEGG pathway enrichment analyses of the differentially expressed metabolites. (G) Sankey diagram of the differentially expressed metabolites and corresponding enrichment pathways. (H) Mechanism diagram of gene–metabolite interaction. Expression levels (I 1 to I 3 ) and quantitative analysis (J 1 to J 3 ) of G6P, FDP, and Acetyl-CoA. P values are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Rabbit Anti Eno1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti eno1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti eno1 - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Plant-Derived Exosomes as Novel Nanotherapeutics Contrive Glycolysis Reprogramming-Mediated Angiogenesis for Diabetic Ulcer Healing"

    Article Title: Plant-Derived Exosomes as Novel Nanotherapeutics Contrive Glycolysis Reprogramming-Mediated Angiogenesis for Diabetic Ulcer Healing

    Journal: Biomaterials Research

    doi: 10.34133/bmr.0035

    HUVECs’ genetic and metabolic alterations to GExos treatment in HG culture. (A1 to A2) Enrichment plots from GSEAs of gene sets for the “Positive regulation of glycolysis signaling”. Expression levels and quantitative analysis of (B1 to B4) PFKM, PGK1, and ENO1, and (C1 to C4) PGLS, ACACA, and PDHA1. (D) Schematic illustration of the major genes and metabolic signaling pathways relevant to angiogenesis, regulated by GExos. (E) Volcano plots of differentially expressed metabolites identified at q < 0.05. (F) KEGG pathway enrichment analyses of the differentially expressed metabolites. (G) Sankey diagram of the differentially expressed metabolites and corresponding enrichment pathways. (H) Mechanism diagram of gene–metabolite interaction. Expression levels (I 1 to I 3 ) and quantitative analysis (J 1 to J 3 ) of G6P, FDP, and Acetyl-CoA. P values are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: HUVECs’ genetic and metabolic alterations to GExos treatment in HG culture. (A1 to A2) Enrichment plots from GSEAs of gene sets for the “Positive regulation of glycolysis signaling”. Expression levels and quantitative analysis of (B1 to B4) PFKM, PGK1, and ENO1, and (C1 to C4) PGLS, ACACA, and PDHA1. (D) Schematic illustration of the major genes and metabolic signaling pathways relevant to angiogenesis, regulated by GExos. (E) Volcano plots of differentially expressed metabolites identified at q < 0.05. (F) KEGG pathway enrichment analyses of the differentially expressed metabolites. (G) Sankey diagram of the differentially expressed metabolites and corresponding enrichment pathways. (H) Mechanism diagram of gene–metabolite interaction. Expression levels (I 1 to I 3 ) and quantitative analysis (J 1 to J 3 ) of G6P, FDP, and Acetyl-CoA. P values are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Expressing

    GExos promote angiogenesis in diabetic ulcers in mice by reprogramming glycolysis. Microvascular imaging (A 1 ) and semi-quantification of the microvascular density (A 2 ) in diabetic healed skins. 8D, 8 days; 16D, 16 days. Immunofluorescence staining (B 1 ) of CD31 (red) and semi-quantification of the area percentage of CD31 (B 2 ) in diabetic healed skins. H&E staining (C) and eNOS staining (D) in diabetic healed skins. Expression levels (E) and quantitative analysis (F 1 to F 3 ) of PFKM, PGK1, and ENO1 on day 8 in diabetic healed skin. Expression levels (G) and quantitative analysis (H 1 to H 3 ) of PFKM, PGK1, and ENO1 on day 16 in diabetic healed skin. Quantitative analysis of G6P (I 1 and I 2 ), FDP (J 1 and J 2 ), and Acetyl-CoA (K 1 and K 2 ) in diabetic healed skins on day 8 and day 16. (L) Schematic diagram of the therapeutic mechanism of GExos promoting angiogenesis in diabetic ulcers. P values are shown: * P < 0.05, ** P < 0.01, **** P < 0.0001.
    Figure Legend Snippet: GExos promote angiogenesis in diabetic ulcers in mice by reprogramming glycolysis. Microvascular imaging (A 1 ) and semi-quantification of the microvascular density (A 2 ) in diabetic healed skins. 8D, 8 days; 16D, 16 days. Immunofluorescence staining (B 1 ) of CD31 (red) and semi-quantification of the area percentage of CD31 (B 2 ) in diabetic healed skins. H&E staining (C) and eNOS staining (D) in diabetic healed skins. Expression levels (E) and quantitative analysis (F 1 to F 3 ) of PFKM, PGK1, and ENO1 on day 8 in diabetic healed skin. Expression levels (G) and quantitative analysis (H 1 to H 3 ) of PFKM, PGK1, and ENO1 on day 16 in diabetic healed skin. Quantitative analysis of G6P (I 1 and I 2 ), FDP (J 1 and J 2 ), and Acetyl-CoA (K 1 and K 2 ) in diabetic healed skins on day 8 and day 16. (L) Schematic diagram of the therapeutic mechanism of GExos promoting angiogenesis in diabetic ulcers. P values are shown: * P < 0.05, ** P < 0.01, **** P < 0.0001.

    Techniques Used: Imaging, Immunofluorescence, Staining, Expressing

    rabbit anti endothelial nitric oxide synthase enos  (Bioss)


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    Bioss rabbit anti endothelial nitric oxide synthase enos
    Rabbit Anti Endothelial Nitric Oxide Synthase Enos, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti endothelial nitric oxide synthase enos/product/Bioss
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti endothelial nitric oxide synthase enos - by Bioz Stars, 2024-07
    93/100 stars

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    Structured Review

    Abcam rabbit monoclonal eno1
    Source, application and concentration of antibodies.
    Rabbit Monoclonal Eno1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal eno1/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal eno1 - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches"

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0291023

    Source, application and concentration of antibodies.
    Figure Legend Snippet: Source, application and concentration of antibodies.

    Techniques Used: Concentration Assay, Recombinant, Plasmid Preparation

    Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.
    Figure Legend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Techniques Used: Mass Spectrometry

    rabbit anti endothelial nitric oxide synthase enos  (Bioss)


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    Bioss rabbit anti endothelial nitric oxide synthase enos
    Representative immunohistochemical photomicrographs of carotid artery sections with (A) VEGF and (C) <t>eNOS</t> staining of each group. Scale bar = 10 µm. (B) The VEGF signal was significantly stronger in the EPC group than in the non-EPC group ( ϕ P = 0.0326, vs non-EPC ). VEGF expression differed significantly between the EPC and EPC groups (** P = 0.0059, vs EPC ) and between the non-EPC and non-EPC groups (# P = 0.0320, vs non-EPC ). (D) eNOS signal intensity was significantly higher in the EPC group than in the non-EPC group ( ϕ P = 0.0260, vs non-EPC ). eNOS expression did differ significantly between the non-EPC and EPC groups ( $$ P = 0.003, vs non-EPC ). eNOS expression was also significantly different between the EPC and EPC groups (* P = 0.0488, vs EPC ). Values are means ± SD. eNOS: endothelial nitric-oxide <t>synthase;</t> EPC: endothelial progenitor cell; VEGF: vascular endothelial growth factor.
    Rabbit Anti Endothelial Nitric Oxide Synthase Enos, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti endothelial nitric oxide synthase enos/product/Bioss
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti endothelial nitric oxide synthase enos - by Bioz Stars, 2024-07
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    1) Product Images from "Human Cord Blood–Endothelial Progenitor Cells Alleviate Intimal Hyperplasia of Arterial Damage in a Rat Stroke Model"

    Article Title: Human Cord Blood–Endothelial Progenitor Cells Alleviate Intimal Hyperplasia of Arterial Damage in a Rat Stroke Model

    Journal: Cell Transplantation

    doi: 10.1177/09636897231193069

    Representative immunohistochemical photomicrographs of carotid artery sections with (A) VEGF and (C) eNOS staining of each group. Scale bar = 10 µm. (B) The VEGF signal was significantly stronger in the EPC group than in the non-EPC group ( ϕ P = 0.0326, vs non-EPC ). VEGF expression differed significantly between the EPC and EPC groups (** P = 0.0059, vs EPC ) and between the non-EPC and non-EPC groups (# P = 0.0320, vs non-EPC ). (D) eNOS signal intensity was significantly higher in the EPC group than in the non-EPC group ( ϕ P = 0.0260, vs non-EPC ). eNOS expression did differ significantly between the non-EPC and EPC groups ( $$ P = 0.003, vs non-EPC ). eNOS expression was also significantly different between the EPC and EPC groups (* P = 0.0488, vs EPC ). Values are means ± SD. eNOS: endothelial nitric-oxide synthase; EPC: endothelial progenitor cell; VEGF: vascular endothelial growth factor.
    Figure Legend Snippet: Representative immunohistochemical photomicrographs of carotid artery sections with (A) VEGF and (C) eNOS staining of each group. Scale bar = 10 µm. (B) The VEGF signal was significantly stronger in the EPC group than in the non-EPC group ( ϕ P = 0.0326, vs non-EPC ). VEGF expression differed significantly between the EPC and EPC groups (** P = 0.0059, vs EPC ) and between the non-EPC and non-EPC groups (# P = 0.0320, vs non-EPC ). (D) eNOS signal intensity was significantly higher in the EPC group than in the non-EPC group ( ϕ P = 0.0260, vs non-EPC ). eNOS expression did differ significantly between the non-EPC and EPC groups ( $$ P = 0.003, vs non-EPC ). eNOS expression was also significantly different between the EPC and EPC groups (* P = 0.0488, vs EPC ). Values are means ± SD. eNOS: endothelial nitric-oxide synthase; EPC: endothelial progenitor cell; VEGF: vascular endothelial growth factor.

    Techniques Used: Immunohistochemistry, Staining, Expressing


    Structured Review

    Proteintech rabbit anti human eno1
    Rabbit Anti Human Eno1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human eno1/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human eno1 - by Bioz Stars, 2024-07
    86/100 stars

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    enos rabbit 1 100 cell signaling  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc enos rabbit 1 100 cell signaling
    Enos Rabbit 1 100 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enos rabbit 1 100 cell signaling/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    enos rabbit 1 100 cell signaling - by Bioz Stars, 2024-07
    86/100 stars

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    eno1 rabbit monoclonal abcam wb  (Danaher Inc)


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    Danaher Inc eno1 rabbit monoclonal abcam wb
    Eno1 Rabbit Monoclonal Abcam Wb, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eno1 rabbit monoclonal abcam wb/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
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    eno1 rabbit monoclonal abcam wb - by Bioz Stars, 2024-07
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    Structured Review

    Proteintech anti eno1 rabbit polyclonal antibody
    <t>ENO1,</t> PFKFB3, NSDHL and SQLE expression in HNSCC. A The typical staining and IHC score of <t>ENO1</t> in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). B The typical staining and IHC score of PFKFB3 in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). C The typical staining and IHC score of NSDHL in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). D The typical staining and IHC score of SQLE in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). Data were shown as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)
    Anti Eno1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti eno1 rabbit polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti eno1 rabbit polyclonal antibody - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Prognosis-related molecular subtyping in head and neck squamous cell carcinoma patients based on glycolytic/cholesterogenic gene data"

    Article Title: Prognosis-related molecular subtyping in head and neck squamous cell carcinoma patients based on glycolytic/cholesterogenic gene data

    Journal: Cancer Cell International

    doi: 10.1186/s12935-023-02880-3

    ENO1, PFKFB3, NSDHL and SQLE expression in HNSCC. A The typical staining and IHC score of ENO1 in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). B The typical staining and IHC score of PFKFB3 in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). C The typical staining and IHC score of NSDHL in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). D The typical staining and IHC score of SQLE in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). Data were shown as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)
    Figure Legend Snippet: ENO1, PFKFB3, NSDHL and SQLE expression in HNSCC. A The typical staining and IHC score of ENO1 in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). B The typical staining and IHC score of PFKFB3 in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). C The typical staining and IHC score of NSDHL in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). D The typical staining and IHC score of SQLE in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). Data were shown as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

    Techniques Used: Expressing, Staining


    Structured Review

    Proteintech anti eno1 rabbit polyclonal antibody
    Anti Eno1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti eno1 rabbit polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
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    anti eno1 rabbit polyclonal antibody - by Bioz Stars, 2024-07
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    Bioss rabbit anti endothelial nitric oxide synthase enos
    Rabbit Anti Endothelial Nitric Oxide Synthase Enos, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti endothelial nitric oxide synthase enos/product/Bioss
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    Danaher Inc rabbit anti human eno1 igg antibody
    Reagents and tools table
    Rabbit Anti Human Eno1 Igg Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human eno1 igg antibody/product/Danaher Inc
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    Danaher Inc rabbit anti eno1
    HUVECs’ genetic and metabolic alterations to GExos treatment in HG culture. (A1 to A2) Enrichment plots from GSEAs of gene sets for the “Positive regulation of glycolysis signaling”. Expression levels and quantitative analysis of (B1 to B4) PFKM, PGK1, and <t>ENO1,</t> and (C1 to C4) PGLS, ACACA, and PDHA1. (D) Schematic illustration of the major genes and metabolic signaling pathways relevant to angiogenesis, regulated by GExos. (E) Volcano plots of differentially expressed metabolites identified at q < 0.05. (F) KEGG pathway enrichment analyses of the differentially expressed metabolites. (G) Sankey diagram of the differentially expressed metabolites and corresponding enrichment pathways. (H) Mechanism diagram of gene–metabolite interaction. Expression levels (I 1 to I 3 ) and quantitative analysis (J 1 to J 3 ) of G6P, FDP, and Acetyl-CoA. P values are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Rabbit Anti Eno1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    <t>ENO1,</t> PFKFB3, NSDHL and SQLE expression in HNSCC. A The typical staining and IHC score of <t>ENO1</t> in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). B The typical staining and IHC score of PFKFB3 in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). C The typical staining and IHC score of NSDHL in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). D The typical staining and IHC score of SQLE in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). Data were shown as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)
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    Image Search Results


    Reagents and tools table

    Journal: Biomarker Research

    Article Title: Single-cell and spatial transcriptomics reveal a high glycolysis B cell and tumor-associated macrophages cluster correlated with poor prognosis and exhausted immune microenvironment in diffuse large B-cell lymphoma

    doi: 10.1186/s40364-024-00605-w

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Rabbit anti-human ENO1 IgG antibody , ab227978 , Abcam.

    Techniques: RNA Sequencing Assay, Sequencing, Expressing, Formalin-fixed Paraffin-Embedded, Immunohistochemistry, Microscopy, Immunofluorescence, Software

    Identifcation of glycolysis / gluconeogenesis maker genes in high malignant B cells. A Volcano plot of differential genes among the benign B cells, low malignant B cells, and high malignant B cells. B. Functional analysis of highly expressed genes in high malignant B cells by Metascape. C. Identification of eight commonly genes overlapped across three groups and glycolysis hallmark genes. D. Module trait correlation showed the relationships between modules, CNV score, and Glycolysis score. E. Network visualization of 10 modules of high maligant B cells.( The modules highlighted in red and underlined are modules associated with CNV score and Glycolysis score. ) F. The first 25 eigengenes of each module. G. Trajectory of different malignant B subclusters predicted by monocle. H. Genes expression level in single spot ordered along the pseudotime for MKI67 and seven glycolysis / gluconeogenesis gene markers ( STMN1, ENO1, LDHA, TPI1, CDK1, PKM , and PPIA ). ( Abbreviation : HMB: high malignant B cells; CNV: copy number variation; UMAP: uniform manifold approximation and projection. *** p < 0.001.)

    Journal: Biomarker Research

    Article Title: Single-cell and spatial transcriptomics reveal a high glycolysis B cell and tumor-associated macrophages cluster correlated with poor prognosis and exhausted immune microenvironment in diffuse large B-cell lymphoma

    doi: 10.1186/s40364-024-00605-w

    Figure Lengend Snippet: Identifcation of glycolysis / gluconeogenesis maker genes in high malignant B cells. A Volcano plot of differential genes among the benign B cells, low malignant B cells, and high malignant B cells. B. Functional analysis of highly expressed genes in high malignant B cells by Metascape. C. Identification of eight commonly genes overlapped across three groups and glycolysis hallmark genes. D. Module trait correlation showed the relationships between modules, CNV score, and Glycolysis score. E. Network visualization of 10 modules of high maligant B cells.( The modules highlighted in red and underlined are modules associated with CNV score and Glycolysis score. ) F. The first 25 eigengenes of each module. G. Trajectory of different malignant B subclusters predicted by monocle. H. Genes expression level in single spot ordered along the pseudotime for MKI67 and seven glycolysis / gluconeogenesis gene markers ( STMN1, ENO1, LDHA, TPI1, CDK1, PKM , and PPIA ). ( Abbreviation : HMB: high malignant B cells; CNV: copy number variation; UMAP: uniform manifold approximation and projection. *** p < 0.001.)

    Article Snippet: Rabbit anti-human ENO1 IgG antibody , ab227978 , Abcam.

    Techniques: Functional Assay, Expressing

    Prognostic value of four glycolysis / gluconeogenesis (STMN1, ENO1, CDK1, PKM, and PPIA) proteins in IHC cohort ( n = 34, 100X) and IFN_TAMs (CD68 + CXCL10 + PD-L1 + ) in mIF cohort ( n = 20, 10X). A-B. Kaplan–Meier curves of OS and PFS according to STMN1, CDK1, ENO1 and PKM proteins expression. C. Representative IHC staining of STMN1, CDK1, ENO1 and PKM in patient 1 (PFS = 7 months, OS = 9 months) and patient 2 (PFS = 135 months, OS = 135 months). D. Kaplan-Meier analysis for PFS based on IFN_TAMs intensity. E. Comparison of IFN_TAMs intensity in relapse and non_relapse groups. F. Correlation of IFN_TAMs’ intensity, CD8 + T cells’ intensity, and TGFβ1 intensity. G. Representative mIF staining of IFN_TAMs and CD8 + T cells in patient #1 (PFS = 2.7 months) and patient #2 (PFS = 90 months). ( Abbreviation : IHC: immunohistochemistry; IFN_TAMs: interferon-primed tumor-associated macrophages; mIF: multiple immunofluorescence; OS: overall survival; PFS: progression -free survival, Relapse: relapsed patients, patients without EFS24; Non_Relapse: non-relapsed patients, patients with EFS24. Mann-Whitney test was performed between groups.)

    Journal: Biomarker Research

    Article Title: Single-cell and spatial transcriptomics reveal a high glycolysis B cell and tumor-associated macrophages cluster correlated with poor prognosis and exhausted immune microenvironment in diffuse large B-cell lymphoma

    doi: 10.1186/s40364-024-00605-w

    Figure Lengend Snippet: Prognostic value of four glycolysis / gluconeogenesis (STMN1, ENO1, CDK1, PKM, and PPIA) proteins in IHC cohort ( n = 34, 100X) and IFN_TAMs (CD68 + CXCL10 + PD-L1 + ) in mIF cohort ( n = 20, 10X). A-B. Kaplan–Meier curves of OS and PFS according to STMN1, CDK1, ENO1 and PKM proteins expression. C. Representative IHC staining of STMN1, CDK1, ENO1 and PKM in patient 1 (PFS = 7 months, OS = 9 months) and patient 2 (PFS = 135 months, OS = 135 months). D. Kaplan-Meier analysis for PFS based on IFN_TAMs intensity. E. Comparison of IFN_TAMs intensity in relapse and non_relapse groups. F. Correlation of IFN_TAMs’ intensity, CD8 + T cells’ intensity, and TGFβ1 intensity. G. Representative mIF staining of IFN_TAMs and CD8 + T cells in patient #1 (PFS = 2.7 months) and patient #2 (PFS = 90 months). ( Abbreviation : IHC: immunohistochemistry; IFN_TAMs: interferon-primed tumor-associated macrophages; mIF: multiple immunofluorescence; OS: overall survival; PFS: progression -free survival, Relapse: relapsed patients, patients without EFS24; Non_Relapse: non-relapsed patients, patients with EFS24. Mann-Whitney test was performed between groups.)

    Article Snippet: Rabbit anti-human ENO1 IgG antibody , ab227978 , Abcam.

    Techniques: Expressing, Immunohistochemistry, Comparison, Staining, Immunofluorescence, MANN-WHITNEY

    HUVECs’ genetic and metabolic alterations to GExos treatment in HG culture. (A1 to A2) Enrichment plots from GSEAs of gene sets for the “Positive regulation of glycolysis signaling”. Expression levels and quantitative analysis of (B1 to B4) PFKM, PGK1, and ENO1, and (C1 to C4) PGLS, ACACA, and PDHA1. (D) Schematic illustration of the major genes and metabolic signaling pathways relevant to angiogenesis, regulated by GExos. (E) Volcano plots of differentially expressed metabolites identified at q < 0.05. (F) KEGG pathway enrichment analyses of the differentially expressed metabolites. (G) Sankey diagram of the differentially expressed metabolites and corresponding enrichment pathways. (H) Mechanism diagram of gene–metabolite interaction. Expression levels (I 1 to I 3 ) and quantitative analysis (J 1 to J 3 ) of G6P, FDP, and Acetyl-CoA. P values are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Biomaterials Research

    Article Title: Plant-Derived Exosomes as Novel Nanotherapeutics Contrive Glycolysis Reprogramming-Mediated Angiogenesis for Diabetic Ulcer Healing

    doi: 10.34133/bmr.0035

    Figure Lengend Snippet: HUVECs’ genetic and metabolic alterations to GExos treatment in HG culture. (A1 to A2) Enrichment plots from GSEAs of gene sets for the “Positive regulation of glycolysis signaling”. Expression levels and quantitative analysis of (B1 to B4) PFKM, PGK1, and ENO1, and (C1 to C4) PGLS, ACACA, and PDHA1. (D) Schematic illustration of the major genes and metabolic signaling pathways relevant to angiogenesis, regulated by GExos. (E) Volcano plots of differentially expressed metabolites identified at q < 0.05. (F) KEGG pathway enrichment analyses of the differentially expressed metabolites. (G) Sankey diagram of the differentially expressed metabolites and corresponding enrichment pathways. (H) Mechanism diagram of gene–metabolite interaction. Expression levels (I 1 to I 3 ) and quantitative analysis (J 1 to J 3 ) of G6P, FDP, and Acetyl-CoA. P values are shown: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20, the membranes were then incubated at 4 °C overnight with the following antibodies: rabbit anti-PFKM (Abcam, ab154804, 1:1,000), rabbit anti-PGK1 (Abcam, ab199438, 1:2,000), rabbit anti-ENO1 (Abcam, ab227978, 1:1,000), rabbit anti-PGLS (Abcam, ab229980, 1:2,000), rabbit anti-ACACA (Abcam, ab45174, 1:2,000), rabbit anti-PDHA1 (Abcam, ab168379, 1:2,000), rabbit anti-β-actin (Abcam, ab68477, 1:10,000, internal control), rabbit anti-Cyclin D1 (Abcam, ab134175, 1:10,000), mouse anti-Cyclin D3 (Abcam, ab289546, 1:1,000), and mouse anti-β-actin (Abcam, ab8226, 1:1,000, internal control).

    Techniques: Expressing

    GExos promote angiogenesis in diabetic ulcers in mice by reprogramming glycolysis. Microvascular imaging (A 1 ) and semi-quantification of the microvascular density (A 2 ) in diabetic healed skins. 8D, 8 days; 16D, 16 days. Immunofluorescence staining (B 1 ) of CD31 (red) and semi-quantification of the area percentage of CD31 (B 2 ) in diabetic healed skins. H&E staining (C) and eNOS staining (D) in diabetic healed skins. Expression levels (E) and quantitative analysis (F 1 to F 3 ) of PFKM, PGK1, and ENO1 on day 8 in diabetic healed skin. Expression levels (G) and quantitative analysis (H 1 to H 3 ) of PFKM, PGK1, and ENO1 on day 16 in diabetic healed skin. Quantitative analysis of G6P (I 1 and I 2 ), FDP (J 1 and J 2 ), and Acetyl-CoA (K 1 and K 2 ) in diabetic healed skins on day 8 and day 16. (L) Schematic diagram of the therapeutic mechanism of GExos promoting angiogenesis in diabetic ulcers. P values are shown: * P < 0.05, ** P < 0.01, **** P < 0.0001.

    Journal: Biomaterials Research

    Article Title: Plant-Derived Exosomes as Novel Nanotherapeutics Contrive Glycolysis Reprogramming-Mediated Angiogenesis for Diabetic Ulcer Healing

    doi: 10.34133/bmr.0035

    Figure Lengend Snippet: GExos promote angiogenesis in diabetic ulcers in mice by reprogramming glycolysis. Microvascular imaging (A 1 ) and semi-quantification of the microvascular density (A 2 ) in diabetic healed skins. 8D, 8 days; 16D, 16 days. Immunofluorescence staining (B 1 ) of CD31 (red) and semi-quantification of the area percentage of CD31 (B 2 ) in diabetic healed skins. H&E staining (C) and eNOS staining (D) in diabetic healed skins. Expression levels (E) and quantitative analysis (F 1 to F 3 ) of PFKM, PGK1, and ENO1 on day 8 in diabetic healed skin. Expression levels (G) and quantitative analysis (H 1 to H 3 ) of PFKM, PGK1, and ENO1 on day 16 in diabetic healed skin. Quantitative analysis of G6P (I 1 and I 2 ), FDP (J 1 and J 2 ), and Acetyl-CoA (K 1 and K 2 ) in diabetic healed skins on day 8 and day 16. (L) Schematic diagram of the therapeutic mechanism of GExos promoting angiogenesis in diabetic ulcers. P values are shown: * P < 0.05, ** P < 0.01, **** P < 0.0001.

    Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20, the membranes were then incubated at 4 °C overnight with the following antibodies: rabbit anti-PFKM (Abcam, ab154804, 1:1,000), rabbit anti-PGK1 (Abcam, ab199438, 1:2,000), rabbit anti-ENO1 (Abcam, ab227978, 1:1,000), rabbit anti-PGLS (Abcam, ab229980, 1:2,000), rabbit anti-ACACA (Abcam, ab45174, 1:2,000), rabbit anti-PDHA1 (Abcam, ab168379, 1:2,000), rabbit anti-β-actin (Abcam, ab68477, 1:10,000, internal control), rabbit anti-Cyclin D1 (Abcam, ab134175, 1:10,000), mouse anti-Cyclin D3 (Abcam, ab289546, 1:1,000), and mouse anti-β-actin (Abcam, ab8226, 1:1,000, internal control).

    Techniques: Imaging, Immunofluorescence, Staining, Expressing

    Source, application and concentration of antibodies.

    Journal: PLOS ONE

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    doi: 10.1371/journal.pone.0291023

    Figure Lengend Snippet: Source, application and concentration of antibodies.

    Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

    Techniques: Concentration Assay, Recombinant, Plasmid Preparation

    Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Journal: PLOS ONE

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    doi: 10.1371/journal.pone.0291023

    Figure Lengend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

    Techniques: Mass Spectrometry

    ENO1, PFKFB3, NSDHL and SQLE expression in HNSCC. A The typical staining and IHC score of ENO1 in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). B The typical staining and IHC score of PFKFB3 in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). C The typical staining and IHC score of NSDHL in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). D The typical staining and IHC score of SQLE in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). Data were shown as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

    Journal: Cancer Cell International

    Article Title: Prognosis-related molecular subtyping in head and neck squamous cell carcinoma patients based on glycolytic/cholesterogenic gene data

    doi: 10.1186/s12935-023-02880-3

    Figure Lengend Snippet: ENO1, PFKFB3, NSDHL and SQLE expression in HNSCC. A The typical staining and IHC score of ENO1 in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). B The typical staining and IHC score of PFKFB3 in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). C The typical staining and IHC score of NSDHL in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). D The typical staining and IHC score of SQLE in in normal tissues and HNSCC tissues from recurrent HNSCC patients (recurrence group) and first diagnosed HNSCC patients surviving at least 5 years (high survival group). Data were shown as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

    Article Snippet: Slides were incubated overnight at 4 °C with the following primary antibodies: anti-ENO1 rabbit polyclonal antibody (1:200; Proteintech, Wuhan, China), anti-PFKFBS rabbit polyclonal antibody (1:200; Proteintech, Wuhan, China), anti-SQLE rabbit polyclonal antibody (1:200; Proteintech, Wuhan, China), anti-NSDHL rabbit polyclonal antibody (1:200; Proteintech, Wuhan, China).

    Techniques: Expressing, Staining