rabbit antirat secondary antibody  (Vector Laboratories)


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    Name:
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed
    Description:
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins Biotinylated Rabbit Anti Rat IgG mouse adsorbed is supplied in solution With some exceptions the recommended dilution for most applications is 1 100 The mouse adsorbed anti rat IgG H L products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross react with mouse immunoglobulins This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues
    Catalog Number:
    ba-4001
    Price:
    None
    Host:
    Rabbit
    Size:
    0 5 mg
    Category:
    Antibodies
    Reactivity:
    Rat
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    Structured Review

    Vector Laboratories rabbit antirat secondary antibody
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins Biotinylated Rabbit Anti Rat IgG mouse adsorbed is supplied in solution With some exceptions the recommended dilution for most applications is 1 100 The mouse adsorbed anti rat IgG H L products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross react with mouse immunoglobulins This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues
    https://www.bioz.com/result/rabbit antirat secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibody - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: CD38 Facilitates Recovery from Traumatic Brain Injury
    Article Snippet: .. The secondary Abs used for 3,3′-diaminobenzidine (DAB) chromogenic staining were as follows: biotin-conjugated rabbit anti-rat diluted 1:500 (for Mac-2 staining) or 1:250 (for F4/80 staining; BA-4001; Vector Laboratories, Burlingame, CA) and biotin-conjugated goat anti-rabbit antibody diluted 1:200 (BA-1000; Vector Laboratories). ..

    Incubation:

    Article Title: Synaptic and non-synaptic localization of protocadherin-?C5 in the rat brain
    Article Snippet: .. The washed sections were incubated with biotinylated anti-rabbit IgG and avidin-biotin-horseradish peroxidase complex (ABC procedure, Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA) in 1% normal goat serum. .. To visualize the reaction, sections were incubated with 3-3′ diaminobenzidine tetrahydrochloride (DAB) in the presence of 0.03% cobalt chloride, 0.03% nickel ammonium sulfate and 0.01% H2 O2 .

    Article Title: Hyperactivation of JAK1 tyrosine kinase induces stepwise, progressive pruritic dermatitis
    Article Snippet: .. Sections were incubated with biotinylated secondary anti-rabbit IgG Ab, followed by an avidin–biotinylated alkaline phosphatase (AP) complex (VECTASTAIN ABC-AP Rabbit IgG Kit; Vector Laboratories; catalog AK-5001). ..

    Article Title: Quercetin Inhibits Angiogenesis Mediated Human Prostate Tumor Growth by Targeting VEGFR- 2 Regulated AKT/mTOR/P70S6K Signaling Pathways
    Article Snippet: .. The sections (5 µm) were blocked with 10% goat serum and incubated with a rabbit anti-CD31 (1∶100; Novus Biologicals Inc, Littleton, CO) and mouse ant-CD34 (1∶100; BD Pharmingen Inc, San Diego, CA) antibodies for 24 h. The slides were subsequently incubated for 30 min with biotinylated anti-rabbit/anti-mouse secondary antibody (Vector laboratories, Burlingame, CA) and followed by incubation of Vectastain ABC Kit (Vector Laboratories). .. Diaminobenzidine (Sigma) was used as the chromagen and methyl green (Sigma) as the counterstain.

    Article Title: Luteolin Inhibits Human Prostate Tumor Growth by Suppressing Vascular Endothelial Growth Factor Receptor 2-Mediated Angiogenesis
    Article Snippet: .. The sections (5 µm) were blocked with 10% goat serum and incubated with a rabbit anti-CD31 (1∶100; Novus Biologicals Inc, Littleton, CO) and mouse ant-CD34 (1∶100; BD Pharmingen Inc, San Diego, CA) antibodies for 24 h. The slides were subsequently incubated for 30 min with biotinylated anti-rabbit/anti-mouse secondary antibody (Vector laboratories, Burlingame, CA) and followed by incubation of Vectastain ABC Kit (Vector Laboratories). .. Diaminobenzidine (Sigma) was used as the chromagen and methyl green (Sigma) as the counterstain.

    Article Title: In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2
    Article Snippet: .. After blocking, slides were incubated with rabbit anti-human active caspase 3 polyclonal Ab (1:1500 dilution, R & D Systems), at 4°C overnight, followed by incubation with biotinylated anti-rabbit Ab and HRP-conjugated streptavidin (both from Vector Laboratories). .. Normal rabbit gamma globulin was used as a negative control (MP Biomedical Services, Santa Ana, CA).

    Article Title: Cytoplasmic and Nuclear Forms of Thyroid Hormone Receptor β1 Are Inversely Associated with Survival in Primary Breast Cancer
    Article Snippet: .. After incubation with a biotinylated secondary anti-rabbit or anti-mouse IgG antibody, and with the associated avidin–biotin–peroxidase complex (both Vectastain Elite ABC Kit; Vector Laboratories, Burlingame, CA, USA), visualization was performed with substrate and chromogen 3, 3-diamino-benzidine (DAB; Dako, Glostrup, Denmark). .. Negative controls (colored in blue) were performed in BC tissue by replacement of the primary antibodies by species-specific (rabbit/mouse) isotype control antibodies (Dako, Glostrup, Denmark).

    Avidin-Biotin Assay:

    Article Title: Synaptic and non-synaptic localization of protocadherin-?C5 in the rat brain
    Article Snippet: .. The washed sections were incubated with biotinylated anti-rabbit IgG and avidin-biotin-horseradish peroxidase complex (ABC procedure, Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA) in 1% normal goat serum. .. To visualize the reaction, sections were incubated with 3-3′ diaminobenzidine tetrahydrochloride (DAB) in the presence of 0.03% cobalt chloride, 0.03% nickel ammonium sulfate and 0.01% H2 O2 .

    Article Title: Hyperactivation of JAK1 tyrosine kinase induces stepwise, progressive pruritic dermatitis
    Article Snippet: .. Sections were incubated with biotinylated secondary anti-rabbit IgG Ab, followed by an avidin–biotinylated alkaline phosphatase (AP) complex (VECTASTAIN ABC-AP Rabbit IgG Kit; Vector Laboratories; catalog AK-5001). ..

    Article Title: Cytoplasmic and Nuclear Forms of Thyroid Hormone Receptor β1 Are Inversely Associated with Survival in Primary Breast Cancer
    Article Snippet: .. After incubation with a biotinylated secondary anti-rabbit or anti-mouse IgG antibody, and with the associated avidin–biotin–peroxidase complex (both Vectastain Elite ABC Kit; Vector Laboratories, Burlingame, CA, USA), visualization was performed with substrate and chromogen 3, 3-diamino-benzidine (DAB; Dako, Glostrup, Denmark). .. Negative controls (colored in blue) were performed in BC tissue by replacement of the primary antibodies by species-specific (rabbit/mouse) isotype control antibodies (Dako, Glostrup, Denmark).

    Immunostaining:

    Article Title: MATRIX METALLOPROTEINASE-13 DEFICIENT MICE ARE RESISTANT TO OSTEOARTHRITIC CARTILAGE EROSION BUT NOT CHONDROCYTE HYPERTROPHY OR OSTEOPHYTE DEVELOPMENT
    Article Snippet: .. Immunostaining was done with a biotinylated anti-rabbit IgG, horse-radish-peroxidase conjugated streptavidin, Nova Red color reagent (Vector Laboratories, CA, USA) and sections were counterstained with Mayers haematoxylin. .. Apoptosis was detected using TUNEL staining (ApopTag, Chemicon, Australia) according to the manufacturer’s instructions.

    Blocking Assay:

    Article Title: In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2
    Article Snippet: .. After blocking, slides were incubated with rabbit anti-human active caspase 3 polyclonal Ab (1:1500 dilution, R & D Systems), at 4°C overnight, followed by incubation with biotinylated anti-rabbit Ab and HRP-conjugated streptavidin (both from Vector Laboratories). .. Normal rabbit gamma globulin was used as a negative control (MP Biomedical Services, Santa Ana, CA).

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  • 99
    Vector Laboratories rabbit antirat igg
    Rabbit Antirat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat igg/product/Vector Laboratories
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories rabbit antirat secondary antibody
    Rabbit Antirat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories rabbit antirat igg biotinylated secondary antibody
    Rabbit Antirat Igg Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat igg biotinylated secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg biotinylated secondary antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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