rabbit antirat secondary antibody  (Vector Laboratories)


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    Name:
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed
    Description:
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins Biotinylated Rabbit Anti Rat IgG mouse adsorbed is supplied in solution With some exceptions the recommended dilution for most applications is 1 100 The mouse adsorbed anti rat IgG H L products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross react with mouse immunoglobulins This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues
    Catalog Number:
    BA-4001
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Rat
    Size:
    0 5 mg
    Host:
    Rabbit
    Buy from Supplier


    Structured Review

    Vector Laboratories rabbit antirat secondary antibody
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed
    Biotinylated Rabbit Anti Rat IgG Antibody mouse adsorbed is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins Biotinylated Rabbit Anti Rat IgG mouse adsorbed is supplied in solution With some exceptions the recommended dilution for most applications is 1 100 The mouse adsorbed anti rat IgG H L products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross react with mouse immunoglobulins This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues
    https://www.bioz.com/result/rabbit antirat secondary antibody/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibody - by Bioz Stars, 2021-07
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Matrilysin [MMP-7] Expression Selects for Cells with Reduced Sensitivity to Apoptosis 1
    Article Snippet: .. The blots were washed and incubated with biotinylated anti-species secondary antibodies (Vector Laboratories, Burlingame, CA). .. After washing, the blots were incubated with streptavidin-HRP (Jackson Immuno-Research, West Grove, PA).

    Article Title: Luteolin Inhibits Human Prostate Tumor Growth by Suppressing Vascular Endothelial Growth Factor Receptor 2-Mediated Angiogenesis
    Article Snippet: Histology and Immunohistochemistry Tumor tissues were fixed in 10% neutral-buffered formalin for 24 hours, processed, and embedded in paraffin blocks. .. The sections (5 µm) were blocked with 10% goat serum and incubated with a rabbit anti-CD31 (1∶100; Novus Biologicals Inc, Littleton, CO) and mouse ant-CD34 (1∶100; BD Pharmingen Inc, San Diego, CA) antibodies for 24 h. The slides were subsequently incubated for 30 min with biotinylated anti-rabbit/anti-mouse secondary antibody (Vector laboratories, Burlingame, CA) and followed by incubation of Vectastain ABC Kit (Vector Laboratories). .. Diaminobenzidine (Sigma) was used as the chromagen and methyl green (Sigma) as the counterstain.

    Article Title: Quercetin Inhibits Angiogenesis Mediated Human Prostate Tumor Growth by Targeting VEGFR- 2 Regulated AKT/mTOR/P70S6K Signaling Pathways
    Article Snippet: Histology and Immunohistochemistry Tumor tissues were fixed in 10% neutral-buffered formalin for 24 hours, processed, and embedded in paraffin blocks. .. The sections (5 µm) were blocked with 10% goat serum and incubated with a rabbit anti-CD31 (1∶100; Novus Biologicals Inc, Littleton, CO) and mouse ant-CD34 (1∶100; BD Pharmingen Inc, San Diego, CA) antibodies for 24 h. The slides were subsequently incubated for 30 min with biotinylated anti-rabbit/anti-mouse secondary antibody (Vector laboratories, Burlingame, CA) and followed by incubation of Vectastain ABC Kit (Vector Laboratories). .. Diaminobenzidine (Sigma) was used as the chromagen and methyl green (Sigma) as the counterstain.

    Article Title: Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters
    Article Snippet: .. Immunohistochemical procedures were as follows: (1) deparaffinization and rehydration, (2) treatment with 0.3% H2 O2 in methanol for 30 min, (3) washing in 0.01 m phosphate-buffered saline (PBS, pH 7.4), (4) blocking with 1.5% normal goat serum/PBS for 60 min, (5) incubation with rabbit antiserum anti-rat ghrelin [13–28] ( ) diluted 1 : 15 000 overnight at 4 °C, (6) washing in PBS, (7) incubation with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, USA) diluted 1 : 200 for 30 min, (8) washing in PBS, (9) incubation with peroxidase-conjugated avidin–biotin complex (Elite ABC kit; Vector Laboratories) for 30 min, (10) washing in PBS, (11) detection of immunoreactivity with a 0.025% 3,3′-diaminobenzidine−0.003% H2 O2 solution, and (12) stopping the reaction with distilled water. ..

    Article Title: Lack of human-like extracellular sortilin neuropathology in transgenic Alzheimer’s disease model mice and macaques
    Article Snippet: For the sections subjected to 6E10 immunolabeling, an antigen retrieval step with formic acid treatment (1 h at room temperature) was applied prior to the aforementioned steps. .. Following incubation with the primary antibodies at 4 °C overnight, the sections were reacted with biotinylated pan-specific secondary antibody (horse anti-mouse, rabbit, and goat immunoglobulin G [IgG]) at 1:400 for 1 h and with avidin-biotin complex reagents (1:400; Vector Laboratories, Burlingame, CA, USA) for another 1 h. The immunoreactive product was visualized in 0.003% H2 O2 and 0.05% 3,3′-diaminobenzidine, with sections mounted on microslides, dehydrated, and coverslipped for light microscopic examination. .. Double immunofluorescence was initiated with treatment of a batch of sections from transgenic mouse, monkey, and human cortex in PBS containing 5% donkey serum for 30 min.

    Immunohistochemistry:

    Article Title: Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters
    Article Snippet: .. Immunohistochemical procedures were as follows: (1) deparaffinization and rehydration, (2) treatment with 0.3% H2 O2 in methanol for 30 min, (3) washing in 0.01 m phosphate-buffered saline (PBS, pH 7.4), (4) blocking with 1.5% normal goat serum/PBS for 60 min, (5) incubation with rabbit antiserum anti-rat ghrelin [13–28] ( ) diluted 1 : 15 000 overnight at 4 °C, (6) washing in PBS, (7) incubation with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, USA) diluted 1 : 200 for 30 min, (8) washing in PBS, (9) incubation with peroxidase-conjugated avidin–biotin complex (Elite ABC kit; Vector Laboratories) for 30 min, (10) washing in PBS, (11) detection of immunoreactivity with a 0.025% 3,3′-diaminobenzidine−0.003% H2 O2 solution, and (12) stopping the reaction with distilled water. ..

    Blocking Assay:

    Article Title: Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters
    Article Snippet: .. Immunohistochemical procedures were as follows: (1) deparaffinization and rehydration, (2) treatment with 0.3% H2 O2 in methanol for 30 min, (3) washing in 0.01 m phosphate-buffered saline (PBS, pH 7.4), (4) blocking with 1.5% normal goat serum/PBS for 60 min, (5) incubation with rabbit antiserum anti-rat ghrelin [13–28] ( ) diluted 1 : 15 000 overnight at 4 °C, (6) washing in PBS, (7) incubation with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, USA) diluted 1 : 200 for 30 min, (8) washing in PBS, (9) incubation with peroxidase-conjugated avidin–biotin complex (Elite ABC kit; Vector Laboratories) for 30 min, (10) washing in PBS, (11) detection of immunoreactivity with a 0.025% 3,3′-diaminobenzidine−0.003% H2 O2 solution, and (12) stopping the reaction with distilled water. ..

    Avidin-Biotin Assay:

    Article Title: Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters
    Article Snippet: .. Immunohistochemical procedures were as follows: (1) deparaffinization and rehydration, (2) treatment with 0.3% H2 O2 in methanol for 30 min, (3) washing in 0.01 m phosphate-buffered saline (PBS, pH 7.4), (4) blocking with 1.5% normal goat serum/PBS for 60 min, (5) incubation with rabbit antiserum anti-rat ghrelin [13–28] ( ) diluted 1 : 15 000 overnight at 4 °C, (6) washing in PBS, (7) incubation with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, USA) diluted 1 : 200 for 30 min, (8) washing in PBS, (9) incubation with peroxidase-conjugated avidin–biotin complex (Elite ABC kit; Vector Laboratories) for 30 min, (10) washing in PBS, (11) detection of immunoreactivity with a 0.025% 3,3′-diaminobenzidine−0.003% H2 O2 solution, and (12) stopping the reaction with distilled water. ..

    Article Title: Lack of human-like extracellular sortilin neuropathology in transgenic Alzheimer’s disease model mice and macaques
    Article Snippet: For the sections subjected to 6E10 immunolabeling, an antigen retrieval step with formic acid treatment (1 h at room temperature) was applied prior to the aforementioned steps. .. Following incubation with the primary antibodies at 4 °C overnight, the sections were reacted with biotinylated pan-specific secondary antibody (horse anti-mouse, rabbit, and goat immunoglobulin G [IgG]) at 1:400 for 1 h and with avidin-biotin complex reagents (1:400; Vector Laboratories, Burlingame, CA, USA) for another 1 h. The immunoreactive product was visualized in 0.003% H2 O2 and 0.05% 3,3′-diaminobenzidine, with sections mounted on microslides, dehydrated, and coverslipped for light microscopic examination. .. Double immunofluorescence was initiated with treatment of a batch of sections from transgenic mouse, monkey, and human cortex in PBS containing 5% donkey serum for 30 min.

    Staining:

    Article Title: CD38 Facilitates Recovery from Traumatic Brain Injury
    Article Snippet: Rabbit anti-glial fibrillary acidic protein (GFAP) polyclonal Abs (Z 0334; Dako Cytomation; Carpinteria, CA) was used for detecting astrocytes, and a biotin-conjugated mouse mAb raised against neuronal nuclei (NeuN) (MAB 377B, Chemicon) was used to detect neurons. .. The secondary Abs used for 3,3′-diaminobenzidine (DAB) chromogenic staining were as follows: biotin-conjugated rabbit anti-rat diluted 1:500 (for Mac-2 staining) or 1:250 (for F4/80 staining; BA-4001; Vector Laboratories, Burlingame, CA) and biotin-conjugated goat anti-rabbit antibody diluted 1:200 (BA-1000; Vector Laboratories). ..

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  • 99
    Vector Laboratories rabbit antirat igg biotinylated secondary antibody
    Rabbit Antirat Igg Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat igg biotinylated secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg biotinylated secondary antibody - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    98
    Vector Laboratories rabbit antirat secondary antibody
    Rabbit Antirat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat secondary antibody/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibody - by Bioz Stars, 2021-07
    98/100 stars
      Buy from Supplier

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