rabbit antirat igg  (Vector Laboratories)


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  • 99
    Name:
    Unconjugated Rabbit Anti Rat IgG Antibody
    Description:
    Unconjugated Rabbit Anti Rat IgG H L Antibody is supplied in carrier free lyophilized form This ultrapure high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination fluorochrome labeling or enzyme conjugations It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier free immunoglobulins
    Catalog Number:
    ai-4000
    Price:
    None
    Host:
    Rabbit
    Size:
    1 5 mg
    Category:
    Antibodies
    Reactivity:
    Rat
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    Structured Review

    Vector Laboratories rabbit antirat igg
    Unconjugated Rabbit Anti Rat IgG Antibody
    Unconjugated Rabbit Anti Rat IgG H L Antibody is supplied in carrier free lyophilized form This ultrapure high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination fluorochrome labeling or enzyme conjugations It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier free immunoglobulins
    https://www.bioz.com/result/rabbit antirat igg/product/Vector Laboratories
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg - by Bioz Stars, 2021-01
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: The TLR7/8 agonist R848 remodels tumor and host responses to promote survival in pancreatic cancer
    Article Snippet: .. Histofine Simple Stain Max Reagents for anti-rat (Nichirei Biosciences, #414311 F) and anti-rabbit (#414144 F) were used for detection of primary antibodies, and the final stain development performed with AEC (Vector Lab, SK-4200). .. Slides were coverslipped in water and scanned following each marker using a Leica Aperio.

    Article Title: Systemic Delivery of a Glucosylceramide Synthase Inhibitor Reduces CNS Substrates and Increases Lifespan in a Mouse Model of Type 2 Gaucher Disease
    Article Snippet: .. Slides were then incubated with a 1∶250 dilution of rabbit anti-rat secondary antibody (Vector laboratories, Burlingame, CA) and stained using the Bond Polymer Refine detection kit (Leica Microsystems, Wetzlar, Germany). .. For each staining technique exposure-matched digital images were obtained from similar brain regions of each experimental group using the Aperio ScanScope XT system (Aperio Technologies, Vista, CA).

    Article Title: Differential Activation of Innate Immune Responses by Adenovirus and Adeno-Associated Virus Vectors
    Article Snippet: .. After being washed with PBS, sections were incubated with rabbit anti-rat immunoglobulin G (IgG) antibody for 30 min at room temperature and then stained using the ABC protocol and DAB substrate (Vector Laboratories). ..

    Incubation:

    Article Title: Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone
    Article Snippet: .. After rinsing in PBS, the sections were incubated with a rat anti-mouse B220 (clone: RA3-6B2, ×200; BD Biosciences) monoclonal antibody, followed by incubation with rabbit anti-rat IgG (Vector Labs, CA, USA) at room temperature. .. Bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ZyMax, South San Francisco, CA) at room temperature.

    Article Title: Systemic Delivery of a Glucosylceramide Synthase Inhibitor Reduces CNS Substrates and Increases Lifespan in a Mouse Model of Type 2 Gaucher Disease
    Article Snippet: .. Slides were then incubated with a 1∶250 dilution of rabbit anti-rat secondary antibody (Vector laboratories, Burlingame, CA) and stained using the Bond Polymer Refine detection kit (Leica Microsystems, Wetzlar, Germany). .. For each staining technique exposure-matched digital images were obtained from similar brain regions of each experimental group using the Aperio ScanScope XT system (Aperio Technologies, Vista, CA).

    Article Title: TLR4 and TLR5 on Corneal Macrophages Regulate Pseudomonas aeruginosa Keratitis by Signaling through MyD88-Dependent and -Independent Pathways
    Article Snippet: .. Sections were washed and incubated with FITC-conjugated rabbit anti-rat Ab (Vector Laboratories) diluted 1/200 in 1% FCS-TBS for 45 min. Neutrophils in the corneal stroma were enumerated directly using fluorescence microscopy (magnification ×40) (Olympus Optical, Tokyo, Japan). .. Corneas were dissected and homogenized in tissue lyser in 200 μl/cornea 0.5% hydratropic acid-Br buffer and 10 mM N- ethylmaleimide (Sigma-Aldrich, St. Louis, MO).

    Article Title: Differential Activation of Innate Immune Responses by Adenovirus and Adeno-Associated Virus Vectors
    Article Snippet: .. After being washed with PBS, sections were incubated with rabbit anti-rat immunoglobulin G (IgG) antibody for 30 min at room temperature and then stained using the ABC protocol and DAB substrate (Vector Laboratories). ..

    Article Title: Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm
    Article Snippet: .. Rat anti-MRP9 mAbs were used in undiluted form (culture supernatant) for 2 h at room temperature; after rinsing with PBS/0.05% Tween 20, the coverslip was pre-incubated with rabbit anti-rat IgG (Vector lab, pre-absorbed with mouse IgG in 1:200 dilution), followed by the polymer–HRP incubation with the AEC substrate, as specified by Dako. ..

    Fluorescence:

    Article Title: TLR4 and TLR5 on Corneal Macrophages Regulate Pseudomonas aeruginosa Keratitis by Signaling through MyD88-Dependent and -Independent Pathways
    Article Snippet: .. Sections were washed and incubated with FITC-conjugated rabbit anti-rat Ab (Vector Laboratories) diluted 1/200 in 1% FCS-TBS for 45 min. Neutrophils in the corneal stroma were enumerated directly using fluorescence microscopy (magnification ×40) (Olympus Optical, Tokyo, Japan). .. Corneas were dissected and homogenized in tissue lyser in 200 μl/cornea 0.5% hydratropic acid-Br buffer and 10 mM N- ethylmaleimide (Sigma-Aldrich, St. Louis, MO).

    Microscopy:

    Article Title: TLR4 and TLR5 on Corneal Macrophages Regulate Pseudomonas aeruginosa Keratitis by Signaling through MyD88-Dependent and -Independent Pathways
    Article Snippet: .. Sections were washed and incubated with FITC-conjugated rabbit anti-rat Ab (Vector Laboratories) diluted 1/200 in 1% FCS-TBS for 45 min. Neutrophils in the corneal stroma were enumerated directly using fluorescence microscopy (magnification ×40) (Olympus Optical, Tokyo, Japan). .. Corneas were dissected and homogenized in tissue lyser in 200 μl/cornea 0.5% hydratropic acid-Br buffer and 10 mM N- ethylmaleimide (Sigma-Aldrich, St. Louis, MO).

    Immunostaining:

    Article Title: Targeting the IL-15 Receptor with an Antagonist IL-15 Mutant/Fc?2a Protein Blocks Delayed-Type Hypersensitivity
    Article Snippet: .. The secondary Ab used for all immunostaining was rabbit anti-rat IgG conjugated with biotin (Vector Laboratories). ..

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  • 99
    Vector Laboratories biotinylated antirat igg
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with <t>antirat</t> or antirabbit <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Biotinylated Antirat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antirat igg/product/Vector Laboratories
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotinylated antirat igg - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories rabbit antirat immunoglobulin g
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with <t>antirat</t> or antirabbit <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Rabbit Antirat Immunoglobulin G, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat immunoglobulin g/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat immunoglobulin g - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories rabbit antirat secondary antibody
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with <t>antirat</t> or antirabbit <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Rabbit Antirat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro 1

    doi:

    Figure Lengend Snippet: TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Article Snippet: Serial sections were stained with antibodies to CD31, CD102, CD144, CD106, Flk-1, and CD62E at 1 µg/ml, detected with biotinylated antirat IgG, and developed with the NovaRED substrate kit (Vector Laboratories).

    Techniques: Staining, Fluorescence, Inverted Microscopy, Software, Generated