rabbit antirat igg  (Abcam)

 
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  • 94
    Name:
    Rabbit Anti Rat IgG H L
    Description:

    Catalog Number:
    ab6703
    Price:
    None
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    Structured Review

    Abcam rabbit antirat igg

    https://www.bioz.com/result/rabbit antirat igg/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Negative Control:

    Article Title: Retrotransposons are specified as DNA replication origins in the gene-poor regions of Arabidopsis heterochromatin
    Article Snippet: .. One μg of soluble chromatin was employed per ChIP reaction, using the following antibodies: anti-H3K9me2 (Abcam ab1220, 3 μg), anti-H3K27me1 (Millipore 07-448, 1 μg), anti-total H3 (Abcam ab1791, 2 μg), or anti-rat IgG (Abcam ab6703, 2 μg) as a negative control. .. Immune complexes were recovered with 50 μl of protein G agarose beads (SCBT) and washed and eluted essentially as described ( ).

    Article Title: Auxin and Epigenetic Regulation of SKP2B, an F-Box That Represses Lateral Root Formation 1, an F-Box That Represses Lateral Root Formation 1 [C], an F-Box That Represses Lateral Root Formation 1 [C] [W], an F-Box That Represses Lateral Root Formation 1 [C] [W] [OA]
    Article Snippet: .. Chromatin was immunoprecipitated with 10 µg of anti-Myc antibody, clone 4A6 (Millipore), or anti-IgG (Abcam ab6703) used as a negative control. .. The different promoter fragments were amplified by PCR and resolved on agarose gels.

    Article Title: Genome-wide analysis of histone H3.1 and H3.3 variants in Arabidopsis thaliana
    Article Snippet: .. Chromatin was sonicated in a Bioruptor (Diagenode) for 30 cycles of 30 s on and 30 s off, and the same amount of chromatin was immunoprecipitated with either 10 μg of anti-Myc antibody (clone 4A6; Millipore) or anti-IgG (ab6703; Abcam) used as a negative control. .. Illumina libraries for sequencing were generated, and the libraries were sequenced using the HiSEq 2000 Sequencing System (Illumina), following the manufacturer's instructions.

    Immunohistochemistry:

    Article Title: The Impact of Hippocampal Sex Hormones Receptors in Modulation of Depressive-Like Behavior Following Chronic Anabolic Androgenic Steroids and Exercise Protocols in Rats
    Article Snippet: .. The slides were then incubated with secondary Rabbit Anti-Rat IgG H & L (1:1,000, ab6703, Abcam, UK) for 30 min. An EXPOSE Rabbit specific HRP/DAB detection IHC Kit (ab80437, Abcam, UK) was used according to the manufacturer’s protocol. .. For ERα staining, the slides were incubated with Anti-ER alpha antibody (E115; 1:200, ab32063, Abcam, UK) overnight at room temperature.

    Immunoprecipitation:

    Article Title: Auxin and Epigenetic Regulation of SKP2B, an F-Box That Represses Lateral Root Formation 1, an F-Box That Represses Lateral Root Formation 1 [C], an F-Box That Represses Lateral Root Formation 1 [C] [W], an F-Box That Represses Lateral Root Formation 1 [C] [W] [OA]
    Article Snippet: .. Chromatin was immunoprecipitated with 10 µg of anti-Myc antibody, clone 4A6 (Millipore), or anti-IgG (Abcam ab6703) used as a negative control. .. The different promoter fragments were amplified by PCR and resolved on agarose gels.

    Article Title: Genome-wide analysis of histone H3.1 and H3.3 variants in Arabidopsis thaliana
    Article Snippet: .. Chromatin was sonicated in a Bioruptor (Diagenode) for 30 cycles of 30 s on and 30 s off, and the same amount of chromatin was immunoprecipitated with either 10 μg of anti-Myc antibody (clone 4A6; Millipore) or anti-IgG (ab6703; Abcam) used as a negative control. .. Illumina libraries for sequencing were generated, and the libraries were sequenced using the HiSEq 2000 Sequencing System (Illumina), following the manufacturer's instructions.

    Incubation:

    Article Title: NLRP3 Inflammasome and Mineralocorticoid Receptors Are Associated with Vascular Dysfunction in Type 2 Diabetes Mellitus
    Article Snippet: .. The secondary antibodies were incubated for 1 h: anti-Rat (1-1000, Sigma-Aldrich, A9037, Saint Louis, MO, USA); anti-Rabbit (1-3000, Abcam, ab6703, Cambridge, UK); and anti-Mouse (1-3000, Abcam, ab6789, Cambridge, UK) signals were revealed through chemiluminescence using Luminata™ Forte Western HRP Substrate (Millipore® , Burlington, Massachusetts, MA, USA), and the images were captured in a ImageQuant 350 Photodocumentation system (GE Healthcare® , Piscata Way, NJ, USA). ..

    Article Title: The inflammasome NLRP3 plays a dual role on mouse corpora cavernosa relaxation
    Article Snippet: .. Membranes were then incubated with the following secondary antibodies: goat anti-mouse IgG H & L (AB6789, diluted 1:10.000, Abcam), goat anti-rabbit IgG H & L (AB6721, diluted 1:10.000, Abcam), rabbit anti-rat IgG (AB6703, diluted 1:3.000, Abcam). .. In vivo measurements of intracavernosal pressure and mean arterial pressure The animals were anesthetized with 2% isoflurane in 100% oxygen (2 L/min).

    Article Title: The Impact of Hippocampal Sex Hormones Receptors in Modulation of Depressive-Like Behavior Following Chronic Anabolic Androgenic Steroids and Exercise Protocols in Rats
    Article Snippet: .. The slides were then incubated with secondary Rabbit Anti-Rat IgG H & L (1:1,000, ab6703, Abcam, UK) for 30 min. An EXPOSE Rabbit specific HRP/DAB detection IHC Kit (ab80437, Abcam, UK) was used according to the manufacturer’s protocol. .. For ERα staining, the slides were incubated with Anti-ER alpha antibody (E115; 1:200, ab32063, Abcam, UK) overnight at room temperature.

    Article Title: Abrogating native α-synuclein tetramers in mice causes a L-DOPA responsive motor syndrome closely resembling Parkinson’s disease
    Article Snippet: .. Sections were washed five times for 10 min i n PBS followed by incubation with rabbit anti-rat bridging antibody (1:100 Abcam ab6703) in PBS containing 1% BSA for 1h at room temperature, then washed five times in PBS, followed by incubation with 15 nm Protein A-gold particles (1:50, Utrecht University Med Ctr) in PBS containing 1% BSA for an hour at room temperature. .. Sections were subsequently washed in PBS and then fixed in 1% (v/v) glutaraldehyde in PBS for 30 min. For Epon embedding, slices were incubated in 0.5% (w/v) osmium in ddH2 O for 30 min, washed three times in ddH2 O then stepwise dehydrated (each step for 10 min) in 70% (v/v) ethanol, 95% (v/v) ethanol and twice in 100% (v/v) ethanol.

    Article Title: Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia
    Article Snippet: .. Those sections were incubated with a rabbit anti-rat IgG primary antibody (ab102168, Abcam). .. Alexa Fluor 488-conjugated goat anti-rabbit IgG antibodies (ab150081, Abcam) were added to the sections followed by incubation.

    Sonication:

    Article Title: Genome-wide analysis of histone H3.1 and H3.3 variants in Arabidopsis thaliana
    Article Snippet: .. Chromatin was sonicated in a Bioruptor (Diagenode) for 30 cycles of 30 s on and 30 s off, and the same amount of chromatin was immunoprecipitated with either 10 μg of anti-Myc antibody (clone 4A6; Millipore) or anti-IgG (ab6703; Abcam) used as a negative control. .. Illumina libraries for sequencing were generated, and the libraries were sequenced using the HiSEq 2000 Sequencing System (Illumina), following the manufacturer's instructions.

    Western Blot:

    Article Title: NLRP3 Inflammasome and Mineralocorticoid Receptors Are Associated with Vascular Dysfunction in Type 2 Diabetes Mellitus
    Article Snippet: .. The secondary antibodies were incubated for 1 h: anti-Rat (1-1000, Sigma-Aldrich, A9037, Saint Louis, MO, USA); anti-Rabbit (1-3000, Abcam, ab6703, Cambridge, UK); and anti-Mouse (1-3000, Abcam, ab6789, Cambridge, UK) signals were revealed through chemiluminescence using Luminata™ Forte Western HRP Substrate (Millipore® , Burlington, Massachusetts, MA, USA), and the images were captured in a ImageQuant 350 Photodocumentation system (GE Healthcare® , Piscata Way, NJ, USA). ..

    Chromatin Immunoprecipitation:

    Article Title: Retrotransposons are specified as DNA replication origins in the gene-poor regions of Arabidopsis heterochromatin
    Article Snippet: .. One μg of soluble chromatin was employed per ChIP reaction, using the following antibodies: anti-H3K9me2 (Abcam ab1220, 3 μg), anti-H3K27me1 (Millipore 07-448, 1 μg), anti-total H3 (Abcam ab1791, 2 μg), or anti-rat IgG (Abcam ab6703, 2 μg) as a negative control. .. Immune complexes were recovered with 50 μl of protein G agarose beads (SCBT) and washed and eluted essentially as described ( ).

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  • 94
    Abcam rabbit antirat igg
    Rabbit Antirat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat igg/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    98
    Abcam antirat igg
    Antirat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirat igg/product/Abcam
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antirat igg - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

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