rabbit antibody against nfatc1  (Cell Signaling Technology Inc)

 
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    Name:
    Rabbit DA1E mAb IgG XP Isotype Control PE Conjugate
    Description:
    This Cell Signaling Technology antibody is conjugated to phycoerythrin PE and tested in house for direct flow cytometry analysis in human cells
    Catalog Number:
    5742
    Price:
    None
    Applications:
    Flow Cytometry
    Category:
    Antibody Conjugates
    Buy from Supplier


    Structured Review

    Cell Signaling Technology Inc rabbit antibody against nfatc1
    Iguratimod blocks PPAR-γ/c-Fos signaling. Proteins were extracted, and the protein expression levels of PPAR-γ, c-Fos and <t>NFATc1</t> were detected (A) and quantified (B). The experiments were repeated 3 times independently. Data are presented as means ± SD. *P
    This Cell Signaling Technology antibody is conjugated to phycoerythrin PE and tested in house for direct flow cytometry analysis in human cells
    https://www.bioz.com/result/rabbit antibody against nfatc1/product/Cell Signaling Technology Inc
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit antibody against nfatc1 - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ"

    Article Title: Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2017.7648

    Iguratimod blocks PPAR-γ/c-Fos signaling. Proteins were extracted, and the protein expression levels of PPAR-γ, c-Fos and NFATc1 were detected (A) and quantified (B). The experiments were repeated 3 times independently. Data are presented as means ± SD. *P
    Figure Legend Snippet: Iguratimod blocks PPAR-γ/c-Fos signaling. Proteins were extracted, and the protein expression levels of PPAR-γ, c-Fos and NFATc1 were detected (A) and quantified (B). The experiments were repeated 3 times independently. Data are presented as means ± SD. *P

    Techniques Used: Expressing

    Iguratimod inhibits the expression of c-Fos, NFATc1 and osteoclast marker genes. (A) The mRNA levels of c-Fos, NFATc1 and osteoclast marker genes were detected using RT-qPCR. Data are presented as means ± SD. (B and C) Proteins were extracted at indicated times and protein expression levels of c-Fos and NFATc1 were detected by western blotting (B) and quantified (C). The experiments were repeated 3 times independently. Data are presented as means ± SD. **P
    Figure Legend Snippet: Iguratimod inhibits the expression of c-Fos, NFATc1 and osteoclast marker genes. (A) The mRNA levels of c-Fos, NFATc1 and osteoclast marker genes were detected using RT-qPCR. Data are presented as means ± SD. (B and C) Proteins were extracted at indicated times and protein expression levels of c-Fos and NFATc1 were detected by western blotting (B) and quantified (C). The experiments were repeated 3 times independently. Data are presented as means ± SD. **P

    Techniques Used: Expressing, Marker, Quantitative RT-PCR, Western Blot

    2) Product Images from "Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis"

    Article Title: Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-29257-1

    The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p
    Figure Legend Snippet: The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p

    Techniques Used: Western Blot, Expressing

    3) Product Images from "Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR"

    Article Title: Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00693

    Meclizine decreases expression of osteoclast maker genes in BMMs. (A,B) Meclizine inhibits RANKL-induced protein expression of NFATc1 and c-Fos. BMMs were treated with or without meclizine (20 μM) in the presence of RANKL (100 ng/mL) and M-CSF (30 ng/ml). Protein expression levels were measured by western blotting at the indicated times and β-actin was used as a loading control. The experiments were repeated three times independently; ∗ P
    Figure Legend Snippet: Meclizine decreases expression of osteoclast maker genes in BMMs. (A,B) Meclizine inhibits RANKL-induced protein expression of NFATc1 and c-Fos. BMMs were treated with or without meclizine (20 μM) in the presence of RANKL (100 ng/mL) and M-CSF (30 ng/ml). Protein expression levels were measured by western blotting at the indicated times and β-actin was used as a loading control. The experiments were repeated three times independently; ∗ P

    Techniques Used: Expressing, Western Blot

    4) Product Images from "Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis"

    Article Title: Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-29257-1

    The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p
    Figure Legend Snippet: The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p

    Techniques Used: Western Blot, Expressing

    Related Articles

    Incubation:

    Article Title: Germinal Center hypoxia and regulation of antibody qualities by a hypoxia response system
    Article Snippet: .. Proteins in whole cell extracts were separated by SDS-PAGE, transferred onto nylon membranes (Millipore), and then incubated with rabbit antibodies against p-S6 (S235/236), p-p70S6K (S371), p-Akt (S473), p-Akt (T308), p-ACC (S79), p-AMPK (T172) (Cell Signaling Technologies), or goat anti-Actin (Santa Cruz) Abs followed by the appropriate fluorophore-conjugated, species-specific secondary anti-Ig antibodies (Rockland Immunochemicals, and LI-COR). .. Proteins were visualized and quantitated by laser excitation and infrared imaging (Odyssey, LI-COR).

    Recombinant:

    Article Title: A short-chain fatty acid, propionate, enhances the cytotoxic effect of cisplatin by modulating GPR41 signaling pathways in HepG2 cells
    Article Snippet: .. Materials (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl) butanamide (CFMB) and TNF-α antagonist R-7050 [ ] (Calbiochem, Darmstadt, Germany); sodium propionate (NaP) (Sigma-Aldrich, St. Louis, MO, USA); pertussis toxin (PTX) (Wako, Osaka, Japan); gallein, cyclopropanecarboxylic acid (CPC) and trichostatin A (TSA) (Tokyo Chemical Industry, Tokyo, Japan); human recombinant TNF-α (PeproTech, Rocky Hill, NJ, USA); polyclonal rabbit antibodies against human β-actin (Abcam), acetyl-histone H3 (Lys9/Lys14), cleaved caspase-3 (Asp175), cleaved caspase-9 (all Cell Signaling Technology, Boston, MA USA), GPR41 (Abcam), and GPR43 (Bioss, Boston, MA, USA); monoclonal rabbit antibodies against HDAC1, HDAC4, HDAC5 (Cell Signaling Technology) and HDAC8 (Abcam); monoclonal mouse antibodies against HDAC3 and caspase-8 (Cell Signaling Technology); and horseradish peroxidase-conjugated anti-mouse, anti-goat and anti-rabbit immunoglobulins (Dako, Glostrup, Denmark) were used in the study. .. Cell cultures HepG2 cells, HuH-7 cells, JHH-4 cells and HLE cells, which are all human hepatoma cell lines, were obtained from the Japanese Collection of Research Bioresources Cell Bank.

    other:

    Article Title: FoxO3 increases miR-34a to cause palmitate-induced cholangiocyte lipoapoptosis
    Article Snippet: Antibodies Rabbit antiserum against FoxO3 (2497), cleaved caspase 3 (C9661), and cleaved poly (ADP-ribose) polymerase (PARP) (P9542) were from Cell Signaling.

    SDS Page:

    Article Title: Germinal Center hypoxia and regulation of antibody qualities by a hypoxia response system
    Article Snippet: .. Proteins in whole cell extracts were separated by SDS-PAGE, transferred onto nylon membranes (Millipore), and then incubated with rabbit antibodies against p-S6 (S235/236), p-p70S6K (S371), p-Akt (S473), p-Akt (T308), p-ACC (S79), p-AMPK (T172) (Cell Signaling Technologies), or goat anti-Actin (Santa Cruz) Abs followed by the appropriate fluorophore-conjugated, species-specific secondary anti-Ig antibodies (Rockland Immunochemicals, and LI-COR). .. Proteins were visualized and quantitated by laser excitation and infrared imaging (Odyssey, LI-COR).

    Similar Products

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  • About
  • News
  • Press Release
  • Team
  • Advisors
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  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Cell Signaling Technology Inc rabbit antibody against nfatc1
    Iguratimod blocks PPAR-γ/c-Fos signaling. Proteins were extracted, and the protein expression levels of PPAR-γ, c-Fos and <t>NFATc1</t> were detected (A) and quantified (B). The experiments were repeated 3 times independently. Data are presented as means ± SD. *P
    Rabbit Antibody Against Nfatc1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibody against nfatc1/product/Cell Signaling Technology Inc
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit antibody against nfatc1 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Iguratimod blocks PPAR-γ/c-Fos signaling. Proteins were extracted, and the protein expression levels of PPAR-γ, c-Fos and NFATc1 were detected (A) and quantified (B). The experiments were repeated 3 times independently. Data are presented as means ± SD. *P

    Journal: Molecular Medicine Reports

    Article Title: Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ

    doi: 10.3892/mmr.2017.7648

    Figure Lengend Snippet: Iguratimod blocks PPAR-γ/c-Fos signaling. Proteins were extracted, and the protein expression levels of PPAR-γ, c-Fos and NFATc1 were detected (A) and quantified (B). The experiments were repeated 3 times independently. Data are presented as means ± SD. *P

    Article Snippet: Rabbit antibody against NFATc1 (no. 8032, dilution 1:1,000) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Expressing

    Iguratimod inhibits the expression of c-Fos, NFATc1 and osteoclast marker genes. (A) The mRNA levels of c-Fos, NFATc1 and osteoclast marker genes were detected using RT-qPCR. Data are presented as means ± SD. (B and C) Proteins were extracted at indicated times and protein expression levels of c-Fos and NFATc1 were detected by western blotting (B) and quantified (C). The experiments were repeated 3 times independently. Data are presented as means ± SD. **P

    Journal: Molecular Medicine Reports

    Article Title: Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ

    doi: 10.3892/mmr.2017.7648

    Figure Lengend Snippet: Iguratimod inhibits the expression of c-Fos, NFATc1 and osteoclast marker genes. (A) The mRNA levels of c-Fos, NFATc1 and osteoclast marker genes were detected using RT-qPCR. Data are presented as means ± SD. (B and C) Proteins were extracted at indicated times and protein expression levels of c-Fos and NFATc1 were detected by western blotting (B) and quantified (C). The experiments were repeated 3 times independently. Data are presented as means ± SD. **P

    Article Snippet: Rabbit antibody against NFATc1 (no. 8032, dilution 1:1,000) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Expressing, Marker, Quantitative RT-PCR, Western Blot

    The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p

    Journal: Scientific Reports

    Article Title: Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis

    doi: 10.1038/s41598-018-29257-1

    Figure Lengend Snippet: The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p

    Article Snippet: Rabbit antibody against NFATc1 (D15F1) was purchased from Cell Signaling (Danvers, MA, United States).

    Techniques: Western Blot, Expressing

    Meclizine decreases expression of osteoclast maker genes in BMMs. (A,B) Meclizine inhibits RANKL-induced protein expression of NFATc1 and c-Fos. BMMs were treated with or without meclizine (20 μM) in the presence of RANKL (100 ng/mL) and M-CSF (30 ng/ml). Protein expression levels were measured by western blotting at the indicated times and β-actin was used as a loading control. The experiments were repeated three times independently; ∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR

    doi: 10.3389/fphar.2017.00693

    Figure Lengend Snippet: Meclizine decreases expression of osteoclast maker genes in BMMs. (A,B) Meclizine inhibits RANKL-induced protein expression of NFATc1 and c-Fos. BMMs were treated with or without meclizine (20 μM) in the presence of RANKL (100 ng/mL) and M-CSF (30 ng/ml). Protein expression levels were measured by western blotting at the indicated times and β-actin was used as a loading control. The experiments were repeated three times independently; ∗ P

    Article Snippet: Rabbit antibody against NFATc1 (D15F1) was purchased from Cell Signaling Technology (Boston, MA, United States).

    Techniques: Expressing, Western Blot

    The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p

    Journal: Scientific Reports

    Article Title: Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis

    doi: 10.1038/s41598-018-29257-1

    Figure Lengend Snippet: The inhibitory effects of NC-8 on the RANKL-induced osteoclast differentiation signaling pathway. ( A and B ) RAW 264.7 cells were treated with 100 ng/ml RANKL or with 10 μg/ml NC-8, and the protein was collected after the reaction. Western blot was used to detect MAPK pathway and downstream NFATc1, c-Fos and NF-κB protein molecules. ( A ) Relative protein expressions analysis of proteins related to the MAPK signaling pathway, including p-ERK, p-JNK, and p-p38. β-actin was used as a loading control. ( B ) Relative protein expressions analysis of proteins related to the NF-κB signaling pathway, including p65, NFATc1 and c-Fos. β-actin was used as a loading control. Relative expression levels of p65, NFATc1 and c-Fos protein in the cytoplasm and in the nuclear fractions. β-actin and HDAC1 were used as loading controls. The results are expressed as the mean ± S.E.M. of four independent experiments. *p

    Article Snippet: Rabbit antibody against NFATc1 (D15F1) was purchased from Cell Signaling (Danvers, MA, United States).

    Techniques: Western Blot, Expressing