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Abcam rabbit antibodies against vegf
Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal <t>(4-HNE)</t> and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor <t>[VEGF],</t> angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4
Rabbit Antibodies Against Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antibodies against vegf/product/Abcam
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
rabbit antibodies against vegf - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis"

Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

Journal: Arthritis Research & Therapy

doi: 10.1186/s13075-018-1592-1

Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4
Figure Legend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4

Techniques Used: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining

4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p
Figure Legend Snippet: 4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p

Techniques Used: Immunofluorescence, Derivative Assay, Activation Assay, Cell Culture

2) Product Images from "Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis"

Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

Journal: Arthritis Research & Therapy

doi: 10.1186/s13075-018-1592-1

Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4
Figure Legend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4

Techniques Used: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining

4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p
Figure Legend Snippet: 4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p

Techniques Used: Immunofluorescence, Derivative Assay, Activation Assay, Cell Culture

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Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis
Article Snippet: .. To visualise immunoexpression of VEGF, cells were fixed in 4% PFA and stained with primary rabbit antibody against VEGF (Abcam). .. To demonstrate ST co-expression of markers of angiogenesis, oxidative stress and bioenergetics, dual-immunofluorescence staining was performed on cryostat synovial sections.

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  • 92
    Abcam rabbit antibodies against vegf
    Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal <t>(4-HNE)</t> and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor <t>[VEGF],</t> angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4
    Rabbit Antibodies Against Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibodies against vegf/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit antibodies against vegf - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Abcam rabbit monoclonal primary antibodies against human vegf
    Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor <t>[VEGF],</t> angiopoietin 2 <t>[Ang2],</t> tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4
    Rabbit Monoclonal Primary Antibodies Against Human Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal primary antibodies against human vegf/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal primary antibodies against human vegf - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Abcam antibodies against vegfr 2
    Vascular endothelial growth factor receptor 2 <t>(VEGFR-2)</t> expression in cerebellar p6, p17 and p30 rats. (A-I) Immunostaining of calbindin positive PCs (red), VEGFR-2 (green) and cell nuclei (blue) in cryosections of rat cerebella at p6, p17, p30. VEGFR-2 receptors are localized within the soma (simple white arrowheads; A–I ) of PCs and in Bergmann glia fibers (white arrows; B ), reaching up to the MCL; exemplarily few cell nuclei are labeled in different cell layers (curved white arrowheads; B,C,E,F,H,I ). Scale bars: (A–I) 20 μm.
    Antibodies Against Vegfr 2, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against vegfr 2/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against vegfr 2 - by Bioz Stars, 2020-09
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    Abcam mouse anti vegf a
    Stimulation of <t>VEGF-A</t> expression in PCa cells by ERRα ( A – B ) Visualization of the overexpression of ERRα protein expression in tumor by IHC on bone lesions in vivo induced by PC3-ERRα (ERRα)(B) compared PC3-CT (CT)(A). ( C – D ) Paralleling the overexpression of ERRα, VEGF-A expression in tumor was also stimulated in vivo in bone lesions induced by PC3-ERRα (ERRα) (D) compared PC3-CT (CT) cells (C). ( E ) Decreased ERRα protein expression by transfection of three pooled siRNA sequences in parental PC3 cells shown by Western blot and ( F – G ) by real-time RT-PCR for ERRα and VEGF-A expression (Student's t -tests P = 0.0002; P = 0.0027). ( H ) Decreased VEGF-A mRNA expression was also observed after XCT-790 treatment at 10 −6 M for 48 h in PC3-ERRα cells (Student's t -tests P = 0.006). ( I – J ) Real-time RT-PCR was performed on triplicate samples and normalized against the ribosomal protein gene L32 to evaluate ERRα ( P = 0.0001) and VEGF-A ( P = 0.0001) expression within subcutaneous (SC) tumors in vivo (pool of n = 3 for each condition). ( K–N ) Similarly, to the PC3 model, IHC revealed that ERRα and VEGF-A protein levels in tumors were increased in mixed lesions induced by PC3c-ERRα (ERRα) (L and N concomitantly) compared to PC3c-CT (CT) (K and M concomitantly) in vivo . Bar = 200 μm, T: Tumor; OB: osteoblasts; BM: Bone Matrix.
    Mouse Anti Vegf A, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti vegf a/product/Abcam
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    Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4

    Journal: Arthritis Research & Therapy

    Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

    doi: 10.1186/s13075-018-1592-1

    Figure Lengend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4

    Article Snippet: ST sections were fixed with acetone for 10 minutes and co-incubated with primary mouse antibody against human 4-HNE (GENTAUR, Kampenhout, Belgium) and with primary rabbit antibodies against VEGF, Ang2, Tie2, ATP5B and glucose transporter 1 (GLUT1) (all from Abcam), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Trevigen, Gaithersburg, MD, USA) and pyruvate kinase isozyme 2 (PKM2) (Abgent, San Diego, CA, USA).

    Techniques: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining

    4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p

    Journal: Arthritis Research & Therapy

    Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

    doi: 10.1186/s13075-018-1592-1

    Figure Lengend Snippet: 4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p

    Article Snippet: ST sections were fixed with acetone for 10 minutes and co-incubated with primary mouse antibody against human 4-HNE (GENTAUR, Kampenhout, Belgium) and with primary rabbit antibodies against VEGF, Ang2, Tie2, ATP5B and glucose transporter 1 (GLUT1) (all from Abcam), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Trevigen, Gaithersburg, MD, USA) and pyruvate kinase isozyme 2 (PKM2) (Abgent, San Diego, CA, USA).

    Techniques: Immunofluorescence, Derivative Assay, Activation Assay, Cell Culture

    Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4

    Journal: Arthritis Research & Therapy

    Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

    doi: 10.1186/s13075-018-1592-1

    Figure Lengend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4

    Article Snippet: The sections were incubated with rabbit monoclonal primary antibodies against human VEGF, Ang2, Tie2, ATP5B (all from Abcam), GAPDH (Trevigen) and mouse monoclonal antibodies against human 4-HNE (GENTAUR).

    Techniques: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining

    Vascular endothelial growth factor receptor 2 (VEGFR-2) expression in cerebellar p6, p17 and p30 rats. (A-I) Immunostaining of calbindin positive PCs (red), VEGFR-2 (green) and cell nuclei (blue) in cryosections of rat cerebella at p6, p17, p30. VEGFR-2 receptors are localized within the soma (simple white arrowheads; A–I ) of PCs and in Bergmann glia fibers (white arrows; B ), reaching up to the MCL; exemplarily few cell nuclei are labeled in different cell layers (curved white arrowheads; B,C,E,F,H,I ). Scale bars: (A–I) 20 μm.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGF

    doi: 10.3389/fnmol.2017.00002

    Figure Lengend Snippet: Vascular endothelial growth factor receptor 2 (VEGFR-2) expression in cerebellar p6, p17 and p30 rats. (A-I) Immunostaining of calbindin positive PCs (red), VEGFR-2 (green) and cell nuclei (blue) in cryosections of rat cerebella at p6, p17, p30. VEGFR-2 receptors are localized within the soma (simple white arrowheads; A–I ) of PCs and in Bergmann glia fibers (white arrows; B ), reaching up to the MCL; exemplarily few cell nuclei are labeled in different cell layers (curved white arrowheads; B,C,E,F,H,I ). Scale bars: (A–I) 20 μm.

    Article Snippet: p6, p17 and p30 Cerebellar Rat Cryosections After blocking and permeabilization, cryosections were incubated with primary antibodies against VEGFR-2 (rabbit, polyclonal, 1:100, ab39256; Abcam) and placed in a fridge (4°C) overnight.

    Techniques: Expressing, Immunostaining, Labeling

    mRNA expression levels in PCs. (A,B) In situ hybridization: expression of VEGFR-2 in rat cerebella at the age of p6 (A) and p30 (B) . Cryosections (15 μm) of each stage were incubated with 80 nM double-DIG-LNA TM probe (Exiqon). In p6 nearly every PC showed a positive VEGFR-2 signal. During development the expression of VEGFR-2 extremely decreases. In p30 VEGFR-2 positive PCs were hardly detectable. P6 and (C) p30 PCs were laser microdissected at 200× magnification and subjected to the method of quantitative polymerase chain reaction (qPCR) (D) . For relative quantification of KDR expression, the 2 −ΔΔCt method was conducted using the housekeeping gene GAPDH for normalization; data are provided as means ± SEM. Data were tested for significance using Student’s t -test. Significant differences are indicated by *** p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGF

    doi: 10.3389/fnmol.2017.00002

    Figure Lengend Snippet: mRNA expression levels in PCs. (A,B) In situ hybridization: expression of VEGFR-2 in rat cerebella at the age of p6 (A) and p30 (B) . Cryosections (15 μm) of each stage were incubated with 80 nM double-DIG-LNA TM probe (Exiqon). In p6 nearly every PC showed a positive VEGFR-2 signal. During development the expression of VEGFR-2 extremely decreases. In p30 VEGFR-2 positive PCs were hardly detectable. P6 and (C) p30 PCs were laser microdissected at 200× magnification and subjected to the method of quantitative polymerase chain reaction (qPCR) (D) . For relative quantification of KDR expression, the 2 −ΔΔCt method was conducted using the housekeeping gene GAPDH for normalization; data are provided as means ± SEM. Data were tested for significance using Student’s t -test. Significant differences are indicated by *** p

    Article Snippet: p6, p17 and p30 Cerebellar Rat Cryosections After blocking and permeabilization, cryosections were incubated with primary antibodies against VEGFR-2 (rabbit, polyclonal, 1:100, ab39256; Abcam) and placed in a fridge (4°C) overnight.

    Techniques: Expressing, In Situ Hybridization, Incubation, Real-time Polymerase Chain Reaction

    Stimulation of VEGF-A expression in PCa cells by ERRα ( A – B ) Visualization of the overexpression of ERRα protein expression in tumor by IHC on bone lesions in vivo induced by PC3-ERRα (ERRα)(B) compared PC3-CT (CT)(A). ( C – D ) Paralleling the overexpression of ERRα, VEGF-A expression in tumor was also stimulated in vivo in bone lesions induced by PC3-ERRα (ERRα) (D) compared PC3-CT (CT) cells (C). ( E ) Decreased ERRα protein expression by transfection of three pooled siRNA sequences in parental PC3 cells shown by Western blot and ( F – G ) by real-time RT-PCR for ERRα and VEGF-A expression (Student's t -tests P = 0.0002; P = 0.0027). ( H ) Decreased VEGF-A mRNA expression was also observed after XCT-790 treatment at 10 −6 M for 48 h in PC3-ERRα cells (Student's t -tests P = 0.006). ( I – J ) Real-time RT-PCR was performed on triplicate samples and normalized against the ribosomal protein gene L32 to evaluate ERRα ( P = 0.0001) and VEGF-A ( P = 0.0001) expression within subcutaneous (SC) tumors in vivo (pool of n = 3 for each condition). ( K–N ) Similarly, to the PC3 model, IHC revealed that ERRα and VEGF-A protein levels in tumors were increased in mixed lesions induced by PC3c-ERRα (ERRα) (L and N concomitantly) compared to PC3c-CT (CT) (K and M concomitantly) in vivo . Bar = 200 μm, T: Tumor; OB: osteoblasts; BM: Bone Matrix.

    Journal: Oncotarget

    Article Title: Estrogen related receptor alpha in castration-resistant prostate cancer cells promotes tumor progression in bone

    doi: 10.18632/oncotarget.12787

    Figure Lengend Snippet: Stimulation of VEGF-A expression in PCa cells by ERRα ( A – B ) Visualization of the overexpression of ERRα protein expression in tumor by IHC on bone lesions in vivo induced by PC3-ERRα (ERRα)(B) compared PC3-CT (CT)(A). ( C – D ) Paralleling the overexpression of ERRα, VEGF-A expression in tumor was also stimulated in vivo in bone lesions induced by PC3-ERRα (ERRα) (D) compared PC3-CT (CT) cells (C). ( E ) Decreased ERRα protein expression by transfection of three pooled siRNA sequences in parental PC3 cells shown by Western blot and ( F – G ) by real-time RT-PCR for ERRα and VEGF-A expression (Student's t -tests P = 0.0002; P = 0.0027). ( H ) Decreased VEGF-A mRNA expression was also observed after XCT-790 treatment at 10 −6 M for 48 h in PC3-ERRα cells (Student's t -tests P = 0.006). ( I – J ) Real-time RT-PCR was performed on triplicate samples and normalized against the ribosomal protein gene L32 to evaluate ERRα ( P = 0.0001) and VEGF-A ( P = 0.0001) expression within subcutaneous (SC) tumors in vivo (pool of n = 3 for each condition). ( K–N ) Similarly, to the PC3 model, IHC revealed that ERRα and VEGF-A protein levels in tumors were increased in mixed lesions induced by PC3c-ERRα (ERRα) (L and N concomitantly) compared to PC3c-CT (CT) (K and M concomitantly) in vivo . Bar = 200 μm, T: Tumor; OB: osteoblasts; BM: Bone Matrix.

    Article Snippet: IHC analysis was performed by incubating sections overnight with rabbit monoclonal against human and mouse anti-VEGF-A (1/50) (Abcam), rabbit polyclonal antibodies against human and mouse WNT5a (1/70)(Abcam), goat anti-mouse postn (1/200)(R & D), mouse monoclonal anti- human/mouse TGFb1 (1/40) (R & D), rabbit polyclonal anti-mouse vimentin(1/50)(Biovision) and mouse monoclonal anti-human/mouse ERRα antibody (1/50)(Santa Cruz) [ ].

    Techniques: Expressing, Over Expression, Immunohistochemistry, In Vivo, Transfection, Western Blot, Quantitative RT-PCR

    Schematic diagram showing ERRα pathways that can mediate tumor progression in bone Pathway 1: Increased expression of pro-resorption factors ( VEGF-A , WNT5A , TGF β 1 ) in PCa tumors overexpressing ERRα leads to direct stimulation of Oc formation. Pathway 2: Increased expression of pro-osteoblastic factors ( VEGF-A , WNT5A , TGF β 1 ) in PCa tumors overexpressing ERRα that leads to direct stimulation of Ob formation. Pathway 3: Indirect stimulation of osteoclasts through osteoblasts (via the regulation of rankl in Ob for the osteolytic model). Pathway 4: Stimulation of the metastatic niche through the stimulation of postn in fibroblasts infiltrating tumors by TGF β 1 secreted by tumor cells. Ob: osteoblast; Oc: osteoclast; direct signaling (in green), indirect signaling (in orange).

    Journal: Oncotarget

    Article Title: Estrogen related receptor alpha in castration-resistant prostate cancer cells promotes tumor progression in bone

    doi: 10.18632/oncotarget.12787

    Figure Lengend Snippet: Schematic diagram showing ERRα pathways that can mediate tumor progression in bone Pathway 1: Increased expression of pro-resorption factors ( VEGF-A , WNT5A , TGF β 1 ) in PCa tumors overexpressing ERRα leads to direct stimulation of Oc formation. Pathway 2: Increased expression of pro-osteoblastic factors ( VEGF-A , WNT5A , TGF β 1 ) in PCa tumors overexpressing ERRα that leads to direct stimulation of Ob formation. Pathway 3: Indirect stimulation of osteoclasts through osteoblasts (via the regulation of rankl in Ob for the osteolytic model). Pathway 4: Stimulation of the metastatic niche through the stimulation of postn in fibroblasts infiltrating tumors by TGF β 1 secreted by tumor cells. Ob: osteoblast; Oc: osteoclast; direct signaling (in green), indirect signaling (in orange).

    Article Snippet: IHC analysis was performed by incubating sections overnight with rabbit monoclonal against human and mouse anti-VEGF-A (1/50) (Abcam), rabbit polyclonal antibodies against human and mouse WNT5a (1/70)(Abcam), goat anti-mouse postn (1/200)(R & D), mouse monoclonal anti- human/mouse TGFb1 (1/40) (R & D), rabbit polyclonal anti-mouse vimentin(1/50)(Biovision) and mouse monoclonal anti-human/mouse ERRα antibody (1/50)(Santa Cruz) [ ].

    Techniques: Expressing