Structured Review

Abcam rabbit antibodies against vegf
Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal <t>(4-HNE)</t> and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor <t>[VEGF],</t> angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4
Rabbit Antibodies Against Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis"

Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

Journal: Arthritis Research & Therapy

doi: 10.1186/s13075-018-1592-1

Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4
Figure Legend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4

Techniques Used: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining

4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p
Figure Legend Snippet: 4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p

Techniques Used: Immunofluorescence, Derivative Assay, Activation Assay, Cell Culture

2) Product Images from "Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis"

Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

Journal: Arthritis Research & Therapy

doi: 10.1186/s13075-018-1592-1

Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4
Figure Legend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets : Figure S4

Techniques Used: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining

4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p
Figure Legend Snippet: 4-Hydroxy-2-nonenal (4-HNE) induces pro-angiogenic and pro-inflammatory mechanisms in primary rheumatoid arthritis synovial fibroblast cells (RASFC). Increased vascular endothelial growth factor (VEGF) immunofluorescence in RASFC subjected to 4-HNE compared to the basal cells and quantification of VEGF, angiopoietin 2 (Ang2), basic fibroblast growth factor (bFGF), interleukin (IL)-8, platelet-derived growth factor subunit B (PDGF-B), regulated on activation, normal T cell expressed and secreted (RANTES), intercellular adhesion molecule (ICAM) in RASFC supernatants ( n = 7) following cell culture with 4-HNE. Data are presented as mean ± SEM. * p

Techniques Used: Immunofluorescence, Derivative Assay, Activation Assay, Cell Culture

Related Articles

Incubation:

Article Title: Vascular endothelial growth factor regulation of endothelial nitric oxide synthase phosphorylation is involved in isoflurane cardiac preconditioning
Article Snippet: .. The membranes were blocked in Tris-buffered saline containing 5% milk and subsequently incubated with primary antibodies against rabbit VEGF (1:1000) (Abcam, Cambridge, MA, USA), VEGF receptor 2 (VEGFR2) (1:500) (Cell signaling, MA, USA), rabbit Akt (Cell Signaling), phosphorylated Akt at the activation site Serine 473 (Cell Signaling), rabbit eNOS (1:200) (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), phosphorylated eNOS at the activation site Serine1177 (p-eNOS) (1:100) (Cell Signaling), and mouse β-actin (1:5000) (Cell signaling) overnight at 4°C. .. The membrane was washed and then incubated with the appropriate anti-rabbit and anti-mouse secondary antibody.

Activation Assay:

Article Title: Vascular endothelial growth factor regulation of endothelial nitric oxide synthase phosphorylation is involved in isoflurane cardiac preconditioning
Article Snippet: .. The membranes were blocked in Tris-buffered saline containing 5% milk and subsequently incubated with primary antibodies against rabbit VEGF (1:1000) (Abcam, Cambridge, MA, USA), VEGF receptor 2 (VEGFR2) (1:500) (Cell signaling, MA, USA), rabbit Akt (Cell Signaling), phosphorylated Akt at the activation site Serine 473 (Cell Signaling), rabbit eNOS (1:200) (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), phosphorylated eNOS at the activation site Serine1177 (p-eNOS) (1:100) (Cell Signaling), and mouse β-actin (1:5000) (Cell signaling) overnight at 4°C. .. The membrane was washed and then incubated with the appropriate anti-rabbit and anti-mouse secondary antibody.

Staining:

Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis
Article Snippet: Immunofluorescence staining of RASFC and synovial tissueSingle-immunofluorescence staining was performed on RASFC following 24-hour cell stimulations with 4-HNE. .. To visualise immunoexpression of VEGF, cells were fixed in 4% PFA and stained with primary rabbit antibody against VEGF (Abcam). .. To demonstrate ST co-expression of markers of angiogenesis, oxidative stress and bioenergetics, dual-immunofluorescence staining was performed on cryostat synovial sections.

other:

Article Title: miR-34a-dependent overexpression of Per1 decreases cholangiocarcinoma growth
Article Snippet: The rabbit polyclonal antibody against VEGF was purchased from Abcam, Cambridge, MA).

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    Abcam rabbit antihuman monoclonal antibody against vegfr 1
    Microphotography of breast cancer tumour with strongly immunohistochemical staining for <t>VEGFR-1</t> (brown staining) (HE; ×40).
    Rabbit Antihuman Monoclonal Antibody Against Vegfr 1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam antibodies against vegfr 2
    Vascular endothelial growth factor receptor 2 <t>(VEGFR-2)</t> expression in cerebellar p6, p17 and p30 rats. (A-I) Immunostaining of calbindin positive PCs (red), VEGFR-2 (green) and cell nuclei (blue) in cryosections of rat cerebella at p6, p17, p30. VEGFR-2 receptors are localized within the soma (simple white arrowheads; A–I ) of PCs and in Bergmann glia fibers (white arrows; B ), reaching up to the MCL; exemplarily few cell nuclei are labeled in different cell layers (curved white arrowheads; B,C,E,F,H,I ). Scale bars: (A–I) 20 μm.
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    Abcam rabbit monoclonal antibody against vegfr1
    <t>VEGFR1</t> acts as a stress-inducible molecule. (A) HUVECs and CT26 tumor cells were incubated with indicated concentrations of cisplatin, doxorubicin, paclitaxel and 5-FU, respectively. (B and C) HUVECs and CT26 tumor cells were irradiated with 2 Gy X-rays
    Rabbit Monoclonal Antibody Against Vegfr1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam monoclonal antibodies against vegfr 3
    Impact of <t>VEGFR-3</t> expression on survivals and clinicopathological characteristics and its clinical significance in patients with iCCA. (A) Patients with VEGFR-3-positive tumors showed more frequently multiple tumor numbers (chi-square-test, p =0.030), lymph node metastasis (chi-square-test, p =0.025) and tumor recurrence (chi-square-test, p
    Monoclonal Antibodies Against Vegfr 3, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Microphotography of breast cancer tumour with strongly immunohistochemical staining for VEGFR-1 (brown staining) (HE; ×40).

    Journal: International Journal of Breast Cancer

    Article Title: Vascular Endothelial Growth Factor Receptor-1 Expression in Breast Cancer and Its Correlation to Vascular Endothelial Growth Factor A

    doi: 10.1155/2013/746749

    Figure Lengend Snippet: Microphotography of breast cancer tumour with strongly immunohistochemical staining for VEGFR-1 (brown staining) (HE; ×40).

    Article Snippet: Dilutions of primary and secondary antibodies were as follows: a mouse antihuman monoclonal antibody against ER, clone NCL-ER-6F11 (Novocastra, UK) in the PBS/BSA buffer, pH = 7.2; a mouse antihuman monoclonal antibody against PR, clone NCL-PGR-312 (Novocastra, UK) dilution 1 : 150 in the PBS/BSA buffer, pH = 7.2; a mouse antihuman monoclonal antibody against VEGF that detects the 121, 165, and 189 VEGF isoforms in routinely fixed specimens, clone VG-1 (Abcam,plc, Cambridge, UK) dilution 1 : 200 in the PBS/BSA with NaN3 buffer; a rabbit antihuman monoclonal antibody against VEGFR-1 that recognises VEGFR-1 and its splice isoform sFlt1 in routinely fixed specimens, clone Y103 (Abcam,plc, Cambridge, UK) dilution 1 : 220 in the PBS/BSA with NaN3 buffer; a goat antimouse polyclonal antibody biotin conjugated (DAKO, Denmark) dilution 1 : 200 in the PBS/BSA buffer, pH = 7.2.

    Techniques: Immunohistochemistry, Staining

    Vascular endothelial growth factor receptor 2 (VEGFR-2) expression in cerebellar p6, p17 and p30 rats. (A-I) Immunostaining of calbindin positive PCs (red), VEGFR-2 (green) and cell nuclei (blue) in cryosections of rat cerebella at p6, p17, p30. VEGFR-2 receptors are localized within the soma (simple white arrowheads; A–I ) of PCs and in Bergmann glia fibers (white arrows; B ), reaching up to the MCL; exemplarily few cell nuclei are labeled in different cell layers (curved white arrowheads; B,C,E,F,H,I ). Scale bars: (A–I) 20 μm.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGF

    doi: 10.3389/fnmol.2017.00002

    Figure Lengend Snippet: Vascular endothelial growth factor receptor 2 (VEGFR-2) expression in cerebellar p6, p17 and p30 rats. (A-I) Immunostaining of calbindin positive PCs (red), VEGFR-2 (green) and cell nuclei (blue) in cryosections of rat cerebella at p6, p17, p30. VEGFR-2 receptors are localized within the soma (simple white arrowheads; A–I ) of PCs and in Bergmann glia fibers (white arrows; B ), reaching up to the MCL; exemplarily few cell nuclei are labeled in different cell layers (curved white arrowheads; B,C,E,F,H,I ). Scale bars: (A–I) 20 μm.

    Article Snippet: p6, p17 and p30 Cerebellar Rat Cryosections After blocking and permeabilization, cryosections were incubated with primary antibodies against VEGFR-2 (rabbit, polyclonal, 1:100, ab39256; Abcam) and placed in a fridge (4°C) overnight.

    Techniques: Expressing, Immunostaining, Labeling

    mRNA expression levels in PCs. (A,B) In situ hybridization: expression of VEGFR-2 in rat cerebella at the age of p6 (A) and p30 (B) . Cryosections (15 μm) of each stage were incubated with 80 nM double-DIG-LNA TM probe (Exiqon). In p6 nearly every PC showed a positive VEGFR-2 signal. During development the expression of VEGFR-2 extremely decreases. In p30 VEGFR-2 positive PCs were hardly detectable. P6 and (C) p30 PCs were laser microdissected at 200× magnification and subjected to the method of quantitative polymerase chain reaction (qPCR) (D) . For relative quantification of KDR expression, the 2 −ΔΔCt method was conducted using the housekeeping gene GAPDH for normalization; data are provided as means ± SEM. Data were tested for significance using Student’s t -test. Significant differences are indicated by *** p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGF

    doi: 10.3389/fnmol.2017.00002

    Figure Lengend Snippet: mRNA expression levels in PCs. (A,B) In situ hybridization: expression of VEGFR-2 in rat cerebella at the age of p6 (A) and p30 (B) . Cryosections (15 μm) of each stage were incubated with 80 nM double-DIG-LNA TM probe (Exiqon). In p6 nearly every PC showed a positive VEGFR-2 signal. During development the expression of VEGFR-2 extremely decreases. In p30 VEGFR-2 positive PCs were hardly detectable. P6 and (C) p30 PCs were laser microdissected at 200× magnification and subjected to the method of quantitative polymerase chain reaction (qPCR) (D) . For relative quantification of KDR expression, the 2 −ΔΔCt method was conducted using the housekeeping gene GAPDH for normalization; data are provided as means ± SEM. Data were tested for significance using Student’s t -test. Significant differences are indicated by *** p

    Article Snippet: p6, p17 and p30 Cerebellar Rat Cryosections After blocking and permeabilization, cryosections were incubated with primary antibodies against VEGFR-2 (rabbit, polyclonal, 1:100, ab39256; Abcam) and placed in a fridge (4°C) overnight.

    Techniques: Expressing, In Situ Hybridization, Incubation, Real-time Polymerase Chain Reaction

    VEGFR1 acts as a stress-inducible molecule. (A) HUVECs and CT26 tumor cells were incubated with indicated concentrations of cisplatin, doxorubicin, paclitaxel and 5-FU, respectively. (B and C) HUVECs and CT26 tumor cells were irradiated with 2 Gy X-rays

    Journal: Experimental and Therapeutic Medicine

    Article Title: Vascular endothelial growth receptor 1 acts as a stress-associated protein in the therapeutic response to thalidomide

    doi: 10.3892/etm.2017.5028

    Figure Lengend Snippet: VEGFR1 acts as a stress-inducible molecule. (A) HUVECs and CT26 tumor cells were incubated with indicated concentrations of cisplatin, doxorubicin, paclitaxel and 5-FU, respectively. (B and C) HUVECs and CT26 tumor cells were irradiated with 2 Gy X-rays

    Article Snippet: Sections were subsequently incubated with rabbit monoclonal antibody against VEGFR1 (ab32152, 1:250 dilution; Abcam) overnight at 4°C, and then washed in PBS (5 min, 3 times; pH 7.6).

    Techniques: Incubation, Irradiation

    Effect of THD-induced VEGFR1 upregulation on ROS. (A and B) CT26 tumor cells and HUVECs were incubated with indicated concentrations of THD for 6 h. Cells were stained with DCFH-DA and ROS levels were evaluated using flow cytometry. (B) HUVECs and CT26

    Journal: Experimental and Therapeutic Medicine

    Article Title: Vascular endothelial growth receptor 1 acts as a stress-associated protein in the therapeutic response to thalidomide

    doi: 10.3892/etm.2017.5028

    Figure Lengend Snippet: Effect of THD-induced VEGFR1 upregulation on ROS. (A and B) CT26 tumor cells and HUVECs were incubated with indicated concentrations of THD for 6 h. Cells were stained with DCFH-DA and ROS levels were evaluated using flow cytometry. (B) HUVECs and CT26

    Article Snippet: Sections were subsequently incubated with rabbit monoclonal antibody against VEGFR1 (ab32152, 1:250 dilution; Abcam) overnight at 4°C, and then washed in PBS (5 min, 3 times; pH 7.6).

    Techniques: Incubation, Staining, Flow Cytometry, Cytometry

    Effect of THD on VEGFR1 protein expression levels in vitro . (A) HUVECs and (B) CT26, (C) HCT116, SW480 and SW620 cells were treated with various concentrations of THD for 24 h. Protein expression levels of VEGFR1 in the various cell types were determined

    Journal: Experimental and Therapeutic Medicine

    Article Title: Vascular endothelial growth receptor 1 acts as a stress-associated protein in the therapeutic response to thalidomide

    doi: 10.3892/etm.2017.5028

    Figure Lengend Snippet: Effect of THD on VEGFR1 protein expression levels in vitro . (A) HUVECs and (B) CT26, (C) HCT116, SW480 and SW620 cells were treated with various concentrations of THD for 24 h. Protein expression levels of VEGFR1 in the various cell types were determined

    Article Snippet: Sections were subsequently incubated with rabbit monoclonal antibody against VEGFR1 (ab32152, 1:250 dilution; Abcam) overnight at 4°C, and then washed in PBS (5 min, 3 times; pH 7.6).

    Techniques: Expressing, In Vitro

    Effect of THD on VEGFR1 protein expression levels in vivo . (A) CT26 cells were implanted into the abdominal cavity of BALB/c mice. Tumor-bearing mice received THD therapy or saline. Body weight, ascites volume and tumor weight were assessed. (B) Representative

    Journal: Experimental and Therapeutic Medicine

    Article Title: Vascular endothelial growth receptor 1 acts as a stress-associated protein in the therapeutic response to thalidomide

    doi: 10.3892/etm.2017.5028

    Figure Lengend Snippet: Effect of THD on VEGFR1 protein expression levels in vivo . (A) CT26 cells were implanted into the abdominal cavity of BALB/c mice. Tumor-bearing mice received THD therapy or saline. Body weight, ascites volume and tumor weight were assessed. (B) Representative

    Article Snippet: Sections were subsequently incubated with rabbit monoclonal antibody against VEGFR1 (ab32152, 1:250 dilution; Abcam) overnight at 4°C, and then washed in PBS (5 min, 3 times; pH 7.6).

    Techniques: Expressing, In Vivo, Mouse Assay

    Impact of VEGFR-3 expression on survivals and clinicopathological characteristics and its clinical significance in patients with iCCA. (A) Patients with VEGFR-3-positive tumors showed more frequently multiple tumor numbers (chi-square-test, p =0.030), lymph node metastasis (chi-square-test, p =0.025) and tumor recurrence (chi-square-test, p

    Journal: International Journal of Biological Sciences

    Article Title: Expression of VEGFR-3 in intrahepatic cholangiocarcinoma correlates with unfavorable prognosis through lymphangiogenesis

    doi: 10.7150/ijbs.26045

    Figure Lengend Snippet: Impact of VEGFR-3 expression on survivals and clinicopathological characteristics and its clinical significance in patients with iCCA. (A) Patients with VEGFR-3-positive tumors showed more frequently multiple tumor numbers (chi-square-test, p =0.030), lymph node metastasis (chi-square-test, p =0.025) and tumor recurrence (chi-square-test, p

    Article Snippet: After antigen retrieval and washing in phosphate-buffered saline, tissue sections were incubated overnight at 4℃ with the monoclonal antibodies against VEGFR-3 (rabbit monoclonal, 1:200; Abcam) and podoplanin (rabbit monoclonal, 1:200; Abcam).

    Techniques: Expressing

    Different expression of VEGFR-3 in intrahepatic cholangiocarcinoma (iCCA). (A) Representative iCCA tissue characterized by a glandular architectural pattern (hematein-eosin-saffron staining). (B) Negative, (C) Weak, (D) Moderate and (E) Strong immunostaining intensity for VEGFR-3 in cancer cells of iCCA tissue. Strong staining intensity for VEGFR-3 in the cytoplasm of cancer cells was regarded as positive controls. (F) Staining for VEGFR-3 was negative in all para-carcinoma tissues and was regarded as negative controls. (G) Of total 106 patients enrolled in the study, 77 patients (72.6%) with iCCA showed positive expression of VEGFR-3 in tumors, while 29 patients (27.4%) presented with negative expression. (scale bar: 200μm)

    Journal: International Journal of Biological Sciences

    Article Title: Expression of VEGFR-3 in intrahepatic cholangiocarcinoma correlates with unfavorable prognosis through lymphangiogenesis

    doi: 10.7150/ijbs.26045

    Figure Lengend Snippet: Different expression of VEGFR-3 in intrahepatic cholangiocarcinoma (iCCA). (A) Representative iCCA tissue characterized by a glandular architectural pattern (hematein-eosin-saffron staining). (B) Negative, (C) Weak, (D) Moderate and (E) Strong immunostaining intensity for VEGFR-3 in cancer cells of iCCA tissue. Strong staining intensity for VEGFR-3 in the cytoplasm of cancer cells was regarded as positive controls. (F) Staining for VEGFR-3 was negative in all para-carcinoma tissues and was regarded as negative controls. (G) Of total 106 patients enrolled in the study, 77 patients (72.6%) with iCCA showed positive expression of VEGFR-3 in tumors, while 29 patients (27.4%) presented with negative expression. (scale bar: 200μm)

    Article Snippet: After antigen retrieval and washing in phosphate-buffered saline, tissue sections were incubated overnight at 4℃ with the monoclonal antibodies against VEGFR-3 (rabbit monoclonal, 1:200; Abcam) and podoplanin (rabbit monoclonal, 1:200; Abcam).

    Techniques: Expressing, Staining, Immunostaining

    Positive expression of VEGFR-3 correlates with tumor-associated lymphangiogenesis in iCCA. Representative tissue sections of iCCA showed low (MLVD=6) (A), median (MLVD=12) (B) and high (MLVD=25) (C) level of tumor-associated lymphangiogenesis assessed by micro-lymphatic vessel density (MLVD). (arrows, micro-lymphatic vessels; scale bar=100μm). (D) In all 106 hepatic resected patients with iCCA, patients with VEGFR-3 positive tumors had significantly higher proportion of MLVD (U-test, p

    Journal: International Journal of Biological Sciences

    Article Title: Expression of VEGFR-3 in intrahepatic cholangiocarcinoma correlates with unfavorable prognosis through lymphangiogenesis

    doi: 10.7150/ijbs.26045

    Figure Lengend Snippet: Positive expression of VEGFR-3 correlates with tumor-associated lymphangiogenesis in iCCA. Representative tissue sections of iCCA showed low (MLVD=6) (A), median (MLVD=12) (B) and high (MLVD=25) (C) level of tumor-associated lymphangiogenesis assessed by micro-lymphatic vessel density (MLVD). (arrows, micro-lymphatic vessels; scale bar=100μm). (D) In all 106 hepatic resected patients with iCCA, patients with VEGFR-3 positive tumors had significantly higher proportion of MLVD (U-test, p

    Article Snippet: After antigen retrieval and washing in phosphate-buffered saline, tissue sections were incubated overnight at 4℃ with the monoclonal antibodies against VEGFR-3 (rabbit monoclonal, 1:200; Abcam) and podoplanin (rabbit monoclonal, 1:200; Abcam).

    Techniques: Expressing, Mann-Whitney U-Test

    Prognostic factors and MLVD in 77 patients with VEGFR-3 positive tumors. (A) For overall survival, tumor size (95%CI: 1.013-3.245, p =0.045) and MLVD analyzed by interquartile range (95%CI: 2.254-10.668, p

    Journal: International Journal of Biological Sciences

    Article Title: Expression of VEGFR-3 in intrahepatic cholangiocarcinoma correlates with unfavorable prognosis through lymphangiogenesis

    doi: 10.7150/ijbs.26045

    Figure Lengend Snippet: Prognostic factors and MLVD in 77 patients with VEGFR-3 positive tumors. (A) For overall survival, tumor size (95%CI: 1.013-3.245, p =0.045) and MLVD analyzed by interquartile range (95%CI: 2.254-10.668, p

    Article Snippet: After antigen retrieval and washing in phosphate-buffered saline, tissue sections were incubated overnight at 4℃ with the monoclonal antibodies against VEGFR-3 (rabbit monoclonal, 1:200; Abcam) and podoplanin (rabbit monoclonal, 1:200; Abcam).

    Techniques: