Structured Review

Cell Signaling Technology Inc rabbit anti phospho ser133 creb
BDNF stimulates <t>CREB</t> phosphorylation via the MAPK signaling pathway. A , left panel , Western blot analysis of <t>phospho-Ser133</t> CREB ( p-CREB ) expression in cortical neurons stimulated with BDNF for various periods of time. Right panel , quantitative analysis. Values represent the mean ± S.E. of five independent experiments and are shown as phospho-CREB expression levels relative to the control ( Ctrl ) value. B , left panel , Western blot analysis of phospho-CREB expression in cortical neurons stimulated with BDNF for 30 min in the presence or absence of the MEK inhibitor U0126. Right panel , quantitative analysis. Values represent the mean ± S.E. of three independent experiments and are shown as phospho-CREB expression levels relative to the control value.
Rabbit Anti Phospho Ser133 Creb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti phospho ser133 creb/product/Cell Signaling Technology Inc
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti phospho ser133 creb - by Bioz Stars, 2019-10
79/100 stars

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Article Title:

Journal:

doi: 10.1074/jbc.M110.125740

BDNF stimulates CREB phosphorylation via the MAPK signaling pathway. A , left panel , Western blot analysis of phospho-Ser133 CREB ( p-CREB ) expression in cortical neurons stimulated with BDNF for various periods of time. Right panel , quantitative analysis. Values represent the mean ± S.E. of five independent experiments and are shown as phospho-CREB expression levels relative to the control ( Ctrl ) value. B , left panel , Western blot analysis of phospho-CREB expression in cortical neurons stimulated with BDNF for 30 min in the presence or absence of the MEK inhibitor U0126. Right panel , quantitative analysis. Values represent the mean ± S.E. of three independent experiments and are shown as phospho-CREB expression levels relative to the control value.
Figure Legend Snippet: BDNF stimulates CREB phosphorylation via the MAPK signaling pathway. A , left panel , Western blot analysis of phospho-Ser133 CREB ( p-CREB ) expression in cortical neurons stimulated with BDNF for various periods of time. Right panel , quantitative analysis. Values represent the mean ± S.E. of five independent experiments and are shown as phospho-CREB expression levels relative to the control ( Ctrl ) value. B , left panel , Western blot analysis of phospho-CREB expression in cortical neurons stimulated with BDNF for 30 min in the presence or absence of the MEK inhibitor U0126. Right panel , quantitative analysis. Values represent the mean ± S.E. of three independent experiments and are shown as phospho-CREB expression levels relative to the control value.

Techniques Used: Western Blot, Expressing

Related Articles

Western Blot:

Article Title:
Article Snippet: To examine the knockdown efficiency of endogenous CRTC1 expression, cortical neurons were transfected with CRTC1 shRNA or Ctrl shRNA, and the ratio of CRTC1 immunofluorescence intensity in the nucleus of the transfected neuron to that in neighboring non-transfected neurons was determined using ImageJ software. .. Western blotting was performed as described previously ( ) with rabbit anti-phospho-Thr202 /Tyr204 p44/42 MAPK (1:1000), rabbit anti-phospho-Ser473 Akt (1:1000), rabbit anti-phospho-Ser133 CREB (1:1000; Cell Signaling Technology), mouse anti-Myc tag (9B11; 1:2000), rabbit anti-CRTC1 (C71D11; 1:1500), and mouse anti-β-tubulin (clone tub 2.1; 1:1000; Sigma) antibodies. .. ECL horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (1:10,000; Amersham Biosciences) was used as a secondary antibody.

Chromatin Immunoprecipitation:

Article Title: A CREB-Mpp7-Amot Regulatory Axis Controls Muscle Stem Cell Expansion and Self-Renewal Competence
Article Snippet: Paragraph title: ChIP-qPCR and qRT-PCR ... Fragmented chromatin was obtained using the truChIP chromatin shearing reagent kit (Covaris) and subjected to IP by rabbit anti-phospho-Ser133-CREB and normal rabbit IgG (Cell Signaling).

Quantitative RT-PCR:

Article Title: A CREB-Mpp7-Amot Regulatory Axis Controls Muscle Stem Cell Expansion and Self-Renewal Competence
Article Snippet: Paragraph title: ChIP-qPCR and qRT-PCR ... Fragmented chromatin was obtained using the truChIP chromatin shearing reagent kit (Covaris) and subjected to IP by rabbit anti-phospho-Ser133-CREB and normal rabbit IgG (Cell Signaling).

Software:

Article Title:
Article Snippet: Western blotting was performed as described previously ( ) with rabbit anti-phospho-Thr202 /Tyr204 p44/42 MAPK (1:1000), rabbit anti-phospho-Ser473 Akt (1:1000), rabbit anti-phospho-Ser133 CREB (1:1000; Cell Signaling Technology), mouse anti-Myc tag (9B11; 1:2000), rabbit anti-CRTC1 (C71D11; 1:1500), and mouse anti-β-tubulin (clone tub 2.1; 1:1000; Sigma) antibodies. .. ECL horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (1:10,000; Amersham Biosciences) was used as a secondary antibody.

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    Cell Signaling Technology Inc affinity purified rabbit monoclonal anti p creb antibody
    <t>anti-P-CREB</t> ser 133 immunostaining difference in A, 57-day-old alcohol exposed mouse hippocampus and B, 57-day-old control mouse hippocampus. Original magnification for anti-P-CREB ser 133 ×40,
    Affinity Purified Rabbit Monoclonal Anti P Creb Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 79/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified rabbit monoclonal anti p creb antibody/product/Cell Signaling Technology Inc
    Average 79 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    affinity purified rabbit monoclonal anti p creb antibody - by Bioz Stars, 2019-10
    79/100 stars
      Buy from Supplier

    Image Search Results


    anti-P-CREB ser 133 immunostaining difference in A, 57-day-old alcohol exposed mouse hippocampus and B, 57-day-old control mouse hippocampus. Original magnification for anti-P-CREB ser 133 ×40,

    Journal:

    Article Title: ALTERATIONS IN P-CREB ACTIVITY: A PATHWAY FOR FETAL ALCOHOL SYNDROME RELATED NEUROTOXICITY

    doi: 10.1016/j.ajog.2008.08.054

    Figure Lengend Snippet: anti-P-CREB ser 133 immunostaining difference in A, 57-day-old alcohol exposed mouse hippocampus and B, 57-day-old control mouse hippocampus. Original magnification for anti-P-CREB ser 133 ×40,

    Article Snippet: Slides with adjacent sections were incubated overnight in 4°C in affinity purified rabbit monoclonal anti P-CREB antibody (1:50 Phospho-CREB (Ser133) (87G3) Rabbit mAb Cell Signaling Technology, Danvers, MA) and then incubated for 30 minutes at room temperature with anti-rabbit IgG biotinylated secondary antibody (1:200).

    Techniques: Immunostaining

    Absolute staining density of anti-P-CREB ser 133 in mice at PND 57 in the hippocampal subregions. Bar represents mean absolute staining ± SE. There is a decreased intensity of staining for anti-P-CREB ser 133r, P

    Journal:

    Article Title: ALTERATIONS IN P-CREB ACTIVITY: A PATHWAY FOR FETAL ALCOHOL SYNDROME RELATED NEUROTOXICITY

    doi: 10.1016/j.ajog.2008.08.054

    Figure Lengend Snippet: Absolute staining density of anti-P-CREB ser 133 in mice at PND 57 in the hippocampal subregions. Bar represents mean absolute staining ± SE. There is a decreased intensity of staining for anti-P-CREB ser 133r, P

    Article Snippet: Slides with adjacent sections were incubated overnight in 4°C in affinity purified rabbit monoclonal anti P-CREB antibody (1:50 Phospho-CREB (Ser133) (87G3) Rabbit mAb Cell Signaling Technology, Danvers, MA) and then incubated for 30 minutes at room temperature with anti-rabbit IgG biotinylated secondary antibody (1:200).

    Techniques: Staining, Mouse Assay

    The influence of PKA activity inhibition on the downstream factor CREB. Phosphorylation level of CREB-Ser133 was detected 15 min after the treatment with 1 mg/ml of LF with or without H-89, a selective PKA inhibitor. Adipocytes were pre-incubated in H-89 starting at 30 min before the addition of LF. Phosphorylation level was normalized to the CREB protein expression level. The statistical significance of the differences in the data for LF treated vs. untreated samples was evaluated using the Student t test. ** p

    Journal: PLoS ONE

    Article Title: Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes

    doi: 10.1371/journal.pone.0141378

    Figure Lengend Snippet: The influence of PKA activity inhibition on the downstream factor CREB. Phosphorylation level of CREB-Ser133 was detected 15 min after the treatment with 1 mg/ml of LF with or without H-89, a selective PKA inhibitor. Adipocytes were pre-incubated in H-89 starting at 30 min before the addition of LF. Phosphorylation level was normalized to the CREB protein expression level. The statistical significance of the differences in the data for LF treated vs. untreated samples was evaluated using the Student t test. ** p

    Article Snippet: The antibodies were used in this study were as follows: anti-adenylyl cyclase 2 (AC2) (C-20) (sc-587, Cosmo Bio Co., Ltd., Tokyo, Japan), anti-AC1 (ab38331, Abcam plc, Cambridge, UK), anti-AC6 (ab14781, Abcam plc, Cambridge, UK), anti-HSL/LIPE (ab45422, Abcam plc, Cambridge, UK), anti-phospho-HSL (Ser660) (#4126, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-c-Raf (#9422, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-phospho-perilipin (Ser497) (4855, VALA Sciences Inc, California, USA), anti-CREB (48H2) (#9197, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-phospho-CREB (Ser133) (87G3) (#9198, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-LRP1(85kDa) (ab92544, Abcam plc, Cambridge, UK), and HRP-conjugated goat anti-rabbit IgG (H+L) (115-035-062, Jackson ImmunoResearch Laboratories Inc., Pennsylvania, USA).

    Techniques: Activity Assay, Inhibition, Incubation, Expressing

    Analysis of the effect of LF on CREB activation. (A) Phosphorylation of CREB-Ser133 in adipocytes treated with LF. Phosphorylated CREB was detected in the presence or absence (0 min) of 1 mg/ml of LF. Phosphorylation levels normalized to the protein expression level of CREB. Changes in protein expression levels of (B) HSL and (C) AC isomers (AC1, 2, and 6) in the presence or absence (0 min) of 1 mg/ml LF normalized to the protein expression level of β-actin. The statistical significance of the data at each sampling time compared with the 0-min sample was evaluated using Dunnett’s multiple comparison test, and the data represent the mean ± SD values of triplicate determinations of one of three identical experiments. * p

    Journal: PLoS ONE

    Article Title: Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes

    doi: 10.1371/journal.pone.0141378

    Figure Lengend Snippet: Analysis of the effect of LF on CREB activation. (A) Phosphorylation of CREB-Ser133 in adipocytes treated with LF. Phosphorylated CREB was detected in the presence or absence (0 min) of 1 mg/ml of LF. Phosphorylation levels normalized to the protein expression level of CREB. Changes in protein expression levels of (B) HSL and (C) AC isomers (AC1, 2, and 6) in the presence or absence (0 min) of 1 mg/ml LF normalized to the protein expression level of β-actin. The statistical significance of the data at each sampling time compared with the 0-min sample was evaluated using Dunnett’s multiple comparison test, and the data represent the mean ± SD values of triplicate determinations of one of three identical experiments. * p

    Article Snippet: The antibodies were used in this study were as follows: anti-adenylyl cyclase 2 (AC2) (C-20) (sc-587, Cosmo Bio Co., Ltd., Tokyo, Japan), anti-AC1 (ab38331, Abcam plc, Cambridge, UK), anti-AC6 (ab14781, Abcam plc, Cambridge, UK), anti-HSL/LIPE (ab45422, Abcam plc, Cambridge, UK), anti-phospho-HSL (Ser660) (#4126, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-c-Raf (#9422, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-phospho-perilipin (Ser497) (4855, VALA Sciences Inc, California, USA), anti-CREB (48H2) (#9197, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-phospho-CREB (Ser133) (87G3) (#9198, Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-LRP1(85kDa) (ab92544, Abcam plc, Cambridge, UK), and HRP-conjugated goat anti-rabbit IgG (H+L) (115-035-062, Jackson ImmunoResearch Laboratories Inc., Pennsylvania, USA).

    Techniques: Activation Assay, Expressing, Sampling