rabbit polyclonal anti α2δ2  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti α2δ2
    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with <t>α2δ2</t> antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Rabbit Polyclonal Anti α2δ2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti α2δ2/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti α2δ2 - by Bioz Stars, 2023-03
    92/100 stars

    Images

    1) Product Images from "Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury"

    Article Title: Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI130391

    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Figure Legend Snippet: (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).

    Techniques Used: Labeling, Fluorescence, Western Blot, Expressing

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).
    Figure Legend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).

    Techniques Used: Fluorescence, Staining, Labeling, Western Blot, Expressing, Cell Culture, Electroporation, In Vitro

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.
    Figure Legend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.

    Techniques Used: Fluorescence, Staining, Labeling, Expressing

    rabbit anti-cacna2d2  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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    Alomone Labs rabbit anti-cacna2d2
    Rabbit Anti Cacna2d2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti-cacna2d2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti-cacna2d2 - by Bioz Stars, 2023-03
    86/100 stars

    Images

    rabbit polyclonal anti α2δ2  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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    • 92
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    Structured Review

    Alomone Labs rabbit polyclonal anti α2δ2
    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with <t>α2δ2</t> antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Rabbit Polyclonal Anti α2δ2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti α2δ2/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti α2δ2 - by Bioz Stars, 2023-03
    92/100 stars

    Images

    1) Product Images from "Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury"

    Article Title: Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI130391

    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Figure Legend Snippet: (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).

    Techniques Used: Labeling, Fluorescence, Western Blot, Expressing

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).
    Figure Legend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).

    Techniques Used: Fluorescence, Staining, Labeling, Western Blot, Expressing, Cell Culture, Electroporation, In Vitro

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.
    Figure Legend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.

    Techniques Used: Fluorescence, Staining, Labeling, Expressing

    rabbit polyclonal anti α2δ2  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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    • 92
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    Structured Review

    Alomone Labs rabbit polyclonal anti α2δ2
    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with <t>α2δ2</t> antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Rabbit Polyclonal Anti α2δ2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti α2δ2/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti α2δ2 - by Bioz Stars, 2023-03
    92/100 stars

    Images

    1) Product Images from "Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury"

    Article Title: Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI130391

    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Figure Legend Snippet: (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).

    Techniques Used: Labeling, Fluorescence, Western Blot, Expressing

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).
    Figure Legend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).

    Techniques Used: Fluorescence, Staining, Labeling, Western Blot, Expressing, Cell Culture, Electroporation, In Vitro

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.
    Figure Legend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.

    Techniques Used: Fluorescence, Staining, Labeling, Expressing

    rat kv3 3 kspitpgsrgrysrdrac rabbit polyclonal alomone acc  (Alomone Labs)


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    Alomone Labs rat kv3 3 kspitpgsrgrysrdrac rabbit polyclonal alomone acc
    Rat Kv3 3 Kspitpgsrgrysrdrac Rabbit Polyclonal Alomone Acc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    • rabbit polyclonal anti α2δ2  (Alomone Labs)
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    Alomone Labs rabbit polyclonal anti α2δ2
    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with <t>α2δ2</t> antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Rabbit Polyclonal Anti α2δ2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti-cacna2d2  (Alomone Labs)
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    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with <t>α2δ2</t> antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
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    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with <t>α2δ2</t> antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).
    Rat Kv3 3 Kspitpgsrgrysrdrac Rabbit Polyclonal Alomone Acc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).

    Journal: The Journal of Clinical Investigation

    Article Title: Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury

    doi: 10.1172/JCI130391

    Figure Lengend Snippet: (A) Schematic of retrograde labeling of corticospinal neurons. (B) Representative fluorescence images of corticospinal neurons identified by retrograde labeling after Fluoro-Gold injections into the cervical spinal cords of adult GFP-M mice. Sagittal sections of the mouse brain were immunostained with α2δ2 antibody (n = 4 independent replicates). Scale bar: 50 μm. (C) Immunoblot shows α2δ2 expression in the mouse sensory-motor cortex during postnatal development. Under reducing conditions, the α2δ2 antibody recognizes 2 bands at approximately 130 and 105 kDa. Tuj1 is used as loading control. (D) Quantification of C. Data normalized using loading control (linear trend test **P < 0.01, n = 3 biological replicates). (E) Representative fluorescence images of corticospinal neurons from mouse brains at different ages. Scale bar: 50 μm. (F) Quantification of E. Box plot (minimum to maximum) and line at median (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; P7 n = 5, P14 n = 3, and P28 n = 3 mice, 60–95 neurons per condition). (G) Raster plots show spontaneous firing within layer V of the sensory-motor cortex at different stages of brain development. (H) Quantification of G. Mean and SEM (linear trend test *P < 0.05; P7 n = 5, P14 n = 7, and P28 n = 8 mice). (I) Schematic representation of C5 SCI experimental model. (J) Representative fluorescence images of retrogradely labeled corticospinal neurons (yellow arrows) 7 days after C5 SCI. DPO, days after operation. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 50 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 4 and SCI n = 4 mice, 229–302 neurons per condition).

    Article Snippet: The following antibodies were used: mouse monoclonal anti-βIII tubulin (Tuj1) (801202, RRID:AB_10063408, BioLegend), rabbit polyclonal anti-βIII tubulin (T2200, RRID:AB_262133, Sigma-Aldrich), mouse monoclonal anti-NeuN (MAB377, RRID:AB_2298772, Millipore), Alexa Fluor 488 Phalloidin (A12379, RRID:AB_2315147, Invitrogen), rabbit polyclonal anti-α2δ2 (ACC-102, RRID:AB_11124467, Alomone Labs), rabbit monoclonal anti–c-Fos (9F6) (2250S, RRID:AB_2247211, Cell Signaling Technology), rabbit polyclonal anti-GFAP (Z0334, RRID:AB_10013382, Dako), rabbit polyclonal anti-mCherry (ab167453, RRID: AB_2571870, Abcam), rabbit monoclonal anti-PKC gamma (59090, RRID: AB_2799557, Cell Signaling Technology), chicken polyclonal anti-Homer1 (160006, RRID:AB_2631222, Synaptic Systems), guinea pig polyclonal anti-VGLUT1 (135304, RRID:AB_887878, Synaptic Systems), rabbit polyclonal anti–Caspase-3 (ab 13847, RRID AB_443014, Abcam),and chicken polyclonal anti-GFP (GFP-1020, RRID:AB_10000240, Aves Labs).

    Techniques: Labeling, Fluorescence, Western Blot, Expressing

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).

    Journal: The Journal of Clinical Investigation

    Article Title: Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury

    doi: 10.1172/JCI130391

    Figure Lengend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from mice with sham operation or unilateral PTX performed at P10. PKCγ staining is shown to confirm lesion completeness. Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; sham n = 6 and PTX n = 5 mice). (F) Representative fluorescence images of corticospinal neurons 32 days after sham or PTX at P10. Sagittal sections of the mouse brain (right hemisphere) were immunostained with α2δ2 antibody. Scale bar: 25 μm. (G) Quantification of F (unpaired 2-tailed Student’s t test **P < 0.01; sham n = 6 and PTX n = 5 mice; 278–369 neurons per condition). (H) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed at P10. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm. (I) Immunoblot showing α2δ2 expression in E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either control (CTR) or Cacna2d2-expressing plasmids. Tuj1 is shown as a loading control (n = 3 independent replicates per condition). (J) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours after electroporation with GFP plus either CTR or Cacna2d2-expressing plasmids. DIV, day in vitro. Scale bar: 100 μm. (K) Quantification of J. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; triplicate experiments; 134–144 neurons per condition). (L) Representative fluorescence images of E17.5 cortical neurons cultured for 48 hours in the presence of either vehicle (0.9% saline) or 250 μM GBP. Scale bar: 100 μm. (M) Quantification of L. Mean and SEM (unpaired 2-tailed Student’s t test ***P < 0.001; 4 independent experiments; 163–165 neurons per condition).

    Article Snippet: The following antibodies were used: mouse monoclonal anti-βIII tubulin (Tuj1) (801202, RRID:AB_10063408, BioLegend), rabbit polyclonal anti-βIII tubulin (T2200, RRID:AB_262133, Sigma-Aldrich), mouse monoclonal anti-NeuN (MAB377, RRID:AB_2298772, Millipore), Alexa Fluor 488 Phalloidin (A12379, RRID:AB_2315147, Invitrogen), rabbit polyclonal anti-α2δ2 (ACC-102, RRID:AB_11124467, Alomone Labs), rabbit monoclonal anti–c-Fos (9F6) (2250S, RRID:AB_2247211, Cell Signaling Technology), rabbit polyclonal anti-GFAP (Z0334, RRID:AB_10013382, Dako), rabbit polyclonal anti-mCherry (ab167453, RRID: AB_2571870, Abcam), rabbit monoclonal anti-PKC gamma (59090, RRID: AB_2799557, Cell Signaling Technology), chicken polyclonal anti-Homer1 (160006, RRID:AB_2631222, Synaptic Systems), guinea pig polyclonal anti-VGLUT1 (135304, RRID:AB_887878, Synaptic Systems), rabbit polyclonal anti–Caspase-3 (ab 13847, RRID AB_443014, Abcam),and chicken polyclonal anti-GFP (GFP-1020, RRID:AB_10000240, Aves Labs).

    Techniques: Fluorescence, Staining, Labeling, Western Blot, Expressing, Cell Culture, Electroporation, In Vitro

    (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.

    Journal: The Journal of Clinical Investigation

    Article Title: Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury

    doi: 10.1172/JCI130391

    Figure Lengend Snippet: (A) Experimental scheme of B. (B) Representative fluorescence images of C7 spinal cord sections from adult mice with unilateral PTX performed at 8 weeks of age. PKCγ staining is shown to confirm lesion completeness. The arrows indicate sprouting corticospinal axons (bottom panels). Scale bar: 500 μm. D, dorsal; V, ventral. (C) Quantification of B. Mean and SEM (unpaired 2-tailed Student’s t test *P < 0.05; vehicle n = 6 and GBP n = 6 mice). (D) Coronal sections of the medullary pyramid showing BDA-labeled corticospinal axons. Scale bar: 50 μm. (E) Quantification of D (unpaired 2-tailed Student’s t test NS, not significant; vehicle n = 6 and GBP n = 6 mice). (F) α2δ2 expression in corticospinal neurons 4 weeks after PTX. Sagittal sections of the mouse brain (left and right hemispheres) were immunostained with α2δ2 antibody. Mean and SEM (1-way ANOVA followed by Dunnett post test *P < 0.05; **P < 0.01; vehicle n = 6 and GBP n = 6 mice, 349–396 neurons per condition). (G) Representative fluorescence images of C7 spinal cord sections from mice with unilateral PTX performed in adulthood treated with GBP. The arrows indicate excitatory synaptic puncta along sprouting corticospinal axons. Inset, 3D reconstruction of the region in the main panel indicated by the yellow arrow. Scale bar: 100 μm.

    Article Snippet: The following antibodies were used: mouse monoclonal anti-βIII tubulin (Tuj1) (801202, RRID:AB_10063408, BioLegend), rabbit polyclonal anti-βIII tubulin (T2200, RRID:AB_262133, Sigma-Aldrich), mouse monoclonal anti-NeuN (MAB377, RRID:AB_2298772, Millipore), Alexa Fluor 488 Phalloidin (A12379, RRID:AB_2315147, Invitrogen), rabbit polyclonal anti-α2δ2 (ACC-102, RRID:AB_11124467, Alomone Labs), rabbit monoclonal anti–c-Fos (9F6) (2250S, RRID:AB_2247211, Cell Signaling Technology), rabbit polyclonal anti-GFAP (Z0334, RRID:AB_10013382, Dako), rabbit polyclonal anti-mCherry (ab167453, RRID: AB_2571870, Abcam), rabbit monoclonal anti-PKC gamma (59090, RRID: AB_2799557, Cell Signaling Technology), chicken polyclonal anti-Homer1 (160006, RRID:AB_2631222, Synaptic Systems), guinea pig polyclonal anti-VGLUT1 (135304, RRID:AB_887878, Synaptic Systems), rabbit polyclonal anti–Caspase-3 (ab 13847, RRID AB_443014, Abcam),and chicken polyclonal anti-GFP (GFP-1020, RRID:AB_10000240, Aves Labs).

    Techniques: Fluorescence, Staining, Labeling, Expressing