Journal: Nanoscale Advances

Article Title: In vitro β-catenin attenuation by a mefloquine-loaded core–shell nano emulsion strategy to suppress liver cancer cells

doi: 10.1039/d4na00547c

Figure Lengend Snippet: Expression studies of the Wnt1 protein towards HepG2 cells. (A) Control group (untreated), (B) treated with MQ/GNE, (C) treated with GNP, (D) treated with MEF/GNE-GNP for 24 h by flow cytometry (Histogram).

Article Snippet: The primary antibodies β-catenin and Wnt1 Rabbit Anti-Human IgG were obtained from Abclonal.

Techniques: Expressing, Control, Flow Cytometry

Journal: Nanoscale Advances

Article Title: In vitro β-catenin attenuation by a mefloquine-loaded core–shell nano emulsion strategy to suppress liver cancer cells

doi: 10.1039/d4na00547c

Figure Lengend Snippet: Expression studies of the Wnt1 protein towards HepG2 cells after treatment.

Article Snippet: The primary antibodies β-catenin and Wnt1 Rabbit Anti-Human IgG were obtained from Abclonal.

Techniques: Expressing

Journal: Oncology Reports

Article Title: Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation

doi: 10.3892/or.2023.8681

Figure Lengend Snippet: Effect of Wnt1-positive expression on proliferative and migratory C6 GSCs in different groups. (A) The positive expression of Wnt1 on C6 GSCs was observed by immunofluorescence staining. Scale bar, 100 µm. (B) The statistical data of the number of Wnt1-positive cells are shown in each group. *P<0.05 and ***P<0.001. GSCs, glioma stem cells; ns, no significance.

Article Snippet: The fixed cells were incubated at 4°C overnight with primary antibodies, including rabbit anti-γ-H 2 AX (1:2,000; cat. no. ab11174; Abcam), rabbit anti-Wnt1 (1:3,000; cat. no. ab15151; Abcam), rabbit anti-Wnt3a (1:2,000; cat. no. ab28472; Abcam) and rabbit anti-β-catenin (1:5,000; cat. no. C2206; MiliporeSigma).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Oncology Reports

Article Title: Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation

doi: 10.3892/or.2023.8681

Figure Lengend Snippet: Changes in Wnt1, Wnt3a and β-catenin protein levels in proliferative and migratory C6 glioma stem cells in different groups. (A) By Western blot analysis, the Wnt1, Wnt3a and β-catenin proteins were detected in the different groups. β-actin was used as the internal control. (B-D) The gray value quantification of (B) Wnt1, (C) Wnt3a and (D) β-catenin protein, normalized to β-actin. *P<0.05, **P<0.01 and ***P<0.001. ns, no significance.

Article Snippet: The fixed cells were incubated at 4°C overnight with primary antibodies, including rabbit anti-γ-H 2 AX (1:2,000; cat. no. ab11174; Abcam), rabbit anti-Wnt1 (1:3,000; cat. no. ab15151; Abcam), rabbit anti-Wnt3a (1:2,000; cat. no. ab28472; Abcam) and rabbit anti-β-catenin (1:5,000; cat. no. C2206; MiliporeSigma).

Techniques: Western Blot