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Cell Signaling Technology Inc rabbit anti vegf
Effect of curcumin and hypoxia on <t>IGF-1R,</t> p-Akt, p-Erk1/2, HIF-1α, <t>VEGF</t> protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P
Rabbit Anti Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti vegf/product/Cell Signaling Technology Inc
Average 99 stars, based on 7 article reviews
Price from $9.99 to $1999.99
rabbit anti vegf - by Bioz Stars, 2020-09
99/100 stars

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1) Product Images from "Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway"

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2018.5783

Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P
Figure Legend Snippet: Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P

Techniques Used: Expressing, Knock-Out, Western Blot

Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.
Figure Legend Snippet: Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.

Techniques Used: Expressing, Inhibition, Translocation Assay

Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P
Figure Legend Snippet: Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P

Techniques Used: Expressing, Knock-Out, Western Blot

2) Product Images from "VEGF silencing inhibits human osteosarcoma angiogenesis and promotes cell apoptosis via PI3K/AKT signaling pathway"

Article Title: VEGF silencing inhibits human osteosarcoma angiogenesis and promotes cell apoptosis via PI3K/AKT signaling pathway

Journal: International Journal of Clinical and Experimental Medicine

doi:

VEGF silencing inhibits the activation of PI3K and AKT in U2OS cells. The levels of p-PI3K, total PI3K, p-AKT and total AKT were detected with western blotting GAPDH was used as a control for normalization of the total protein loading.
Figure Legend Snippet: VEGF silencing inhibits the activation of PI3K and AKT in U2OS cells. The levels of p-PI3K, total PI3K, p-AKT and total AKT were detected with western blotting GAPDH was used as a control for normalization of the total protein loading.

Techniques Used: Activation Assay, Western Blot

3) Product Images from "The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis"

Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2018.6397

The correlation analysis between the relative protein expression level of Wnt1 and that of VEGF in the aorta of the rats. The protein expression level of Wnt1 in the aorta of the rats is positively correlated with that of VEGF (P
Figure Legend Snippet: The correlation analysis between the relative protein expression level of Wnt1 and that of VEGF in the aorta of the rats. The protein expression level of Wnt1 in the aorta of the rats is positively correlated with that of VEGF (P

Techniques Used: Expressing

Detection of relevant protein expression levels in the aorta of the rats in each group with western blot analysis. (A) Strip figure. (B) Relative protein expression level of Wnt1. (C) Relative protein expression level of β-catenin. (D) Relative protein expression level of DKK1. (E) Relative protein expression level of VEGF. The protein expression levels of Wnt1, β-catenin, DKK1 and VEGF in the aorta of the rats in the model group are significantly higher than those of the rats in the control group (**P
Figure Legend Snippet: Detection of relevant protein expression levels in the aorta of the rats in each group with western blot analysis. (A) Strip figure. (B) Relative protein expression level of Wnt1. (C) Relative protein expression level of β-catenin. (D) Relative protein expression level of DKK1. (E) Relative protein expression level of VEGF. The protein expression levels of Wnt1, β-catenin, DKK1 and VEGF in the aorta of the rats in the model group are significantly higher than those of the rats in the control group (**P

Techniques Used: Expressing, Western Blot, Stripping Membranes

4) Product Images from "Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway"

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2018.5783

Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P
Figure Legend Snippet: Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P

Techniques Used: Expressing, Knock-Out, Western Blot

Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.
Figure Legend Snippet: Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.

Techniques Used: Expressing, Inhibition, Translocation Assay

Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P
Figure Legend Snippet: Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P

Techniques Used: Expressing, Knock-Out, Western Blot

5) Product Images from "Hyperactivated FRS2α-mediated signaling in prostate cancer cells promotes tumor angiogenesis and predicts poor clinical outcome of patients"

Article Title: Hyperactivated FRS2α-mediated signaling in prostate cancer cells promotes tumor angiogenesis and predicts poor clinical outcome of patients

Journal: Oncogene

doi: 10.1038/onc.2015.239

Depletion of FRS2 α reduces expression of Vegf-a in mouse TRAMP tumors A. Real-time RT-PCR analyses of the indicated gene expression in TRAMP tumors with or without tissue-specific ablation of Frs2α in the epithelial cells. B C . Immunostaining (B) and Western blot (C) analyses of cJUN and HIF1α expression in the TRAMP tumors. F/F, Frs2α flox ; CN, Frs2α CN ; *, P
Figure Legend Snippet: Depletion of FRS2 α reduces expression of Vegf-a in mouse TRAMP tumors A. Real-time RT-PCR analyses of the indicated gene expression in TRAMP tumors with or without tissue-specific ablation of Frs2α in the epithelial cells. B C . Immunostaining (B) and Western blot (C) analyses of cJUN and HIF1α expression in the TRAMP tumors. F/F, Frs2α flox ; CN, Frs2α CN ; *, P

Techniques Used: Expressing, Quantitative RT-PCR, Immunostaining, Western Blot

6) Product Images from "The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis"

Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2018.6397

The correlation analysis between the relative protein expression level of Wnt1 and that of VEGF in the aorta of the rats. The protein expression level of Wnt1 in the aorta of the rats is positively correlated with that of VEGF (P
Figure Legend Snippet: The correlation analysis between the relative protein expression level of Wnt1 and that of VEGF in the aorta of the rats. The protein expression level of Wnt1 in the aorta of the rats is positively correlated with that of VEGF (P

Techniques Used: Expressing

Detection of relevant protein expression levels in the aorta of the rats in each group with western blot analysis. (A) Strip figure. (B) Relative protein expression level of Wnt1. (C) Relative protein expression level of β-catenin. (D) Relative protein expression level of DKK1. (E) Relative protein expression level of VEGF. The protein expression levels of Wnt1, β-catenin, DKK1 and VEGF in the aorta of the rats in the model group are significantly higher than those of the rats in the control group (**P
Figure Legend Snippet: Detection of relevant protein expression levels in the aorta of the rats in each group with western blot analysis. (A) Strip figure. (B) Relative protein expression level of Wnt1. (C) Relative protein expression level of β-catenin. (D) Relative protein expression level of DKK1. (E) Relative protein expression level of VEGF. The protein expression levels of Wnt1, β-catenin, DKK1 and VEGF in the aorta of the rats in the model group are significantly higher than those of the rats in the control group (**P

Techniques Used: Expressing, Western Blot, Stripping Membranes

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Incubation:

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    Cell Signaling Technology Inc rabbit anti vegf
    Effect of curcumin and hypoxia on <t>IGF-1R,</t> p-Akt, p-Erk1/2, HIF-1α, <t>VEGF</t> protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P
    Rabbit Anti Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vegf/product/Cell Signaling Technology Inc
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rabbit anti vegf - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    Cell Signaling Technology Inc rabbit anti vegf receptor 2
    Gene expression analysis of GEnCs cultured on hydrogel substrates at designated time points. (A)  PECAM1  encoding for platelet endothelial cell adhesion molecule or CD31. (B)  CDH5  encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C)  ITGB1  encoding for integrin subunit β1. (D)  ITGB3  encoding for integrin subunit β3. (E)  KDR  encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F)  TEK  encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G)  VWF  encoding for von Willebrand factor. (H)  NOS3  encoding for nitric oxide synthase 3. (I)  PLVAP  encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression  normalized to gene expression of GEnCs cultured in tissue culture polystyrene on day 0 ( n  = 4).
    Rabbit Anti Vegf Receptor 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti vegf receptor 2 - by Bioz Stars, 2020-09
    85/100 stars
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    86
    Cell Signaling Technology Inc rabbit monoclonal anti vegfr 2
    VEGF-A secreted by BM-MSC activate LEC. (A) Western blot analyses of VEGF-A and VEGF-C production on serum-free EBM-2 (CTR) and MSC conditioned medium (MSC CM). (B) <t>VEGFR-2</t> (top) and VEGFR-3 (bottom) proteins were detected following a phosphorylated tyrosine-containing protein (pY) immunoprecipation (IP) of LEC lysates after cell stimulation with control medium (CTR) or with MSC conditioned medium (MSC CM). Cells treated with VEGF-A (10 ng/ml) or VEGF-C (400 ng/ml) were used as negative and positive controls, respectively. GAPDH western blot was performed on the flowthrough of each sample.
    Rabbit Monoclonal Anti Vegfr 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti vegfr 2/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti vegfr 2 - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

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    Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

    doi: 10.3892/etm.2018.5783

    Figure Lengend Snippet: Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P

    Article Snippet: The primary antibodies used in the experiment were as follows: Rabbit anti-IGF-1R (cat. no. 9750), rabbit anti-HIF-1α (cat. no. 3716), rabbit anti-VEGF (cat. no. 2463), rabbit anti-Akt (cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (cat. no. 5012), rabbit anti-extracellular signal-regulated kinases (Erk1/2; cat. no. 4695), rabbit anti-p-Erk1/2 (cat. no. 4377) (all 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) and rabbit anti-β-actin (cat. no. sc-7210; 1:4,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Expressing, Knock-Out, Western Blot

    Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

    doi: 10.3892/etm.2018.5783

    Figure Lengend Snippet: Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.

    Article Snippet: The primary antibodies used in the experiment were as follows: Rabbit anti-IGF-1R (cat. no. 9750), rabbit anti-HIF-1α (cat. no. 3716), rabbit anti-VEGF (cat. no. 2463), rabbit anti-Akt (cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (cat. no. 5012), rabbit anti-extracellular signal-regulated kinases (Erk1/2; cat. no. 4695), rabbit anti-p-Erk1/2 (cat. no. 4377) (all 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) and rabbit anti-β-actin (cat. no. sc-7210; 1:4,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Expressing, Inhibition, Translocation Assay

    Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

    doi: 10.3892/etm.2018.5783

    Figure Lengend Snippet: Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P

    Article Snippet: The primary antibodies used in the experiment were as follows: Rabbit anti-IGF-1R (cat. no. 9750), rabbit anti-HIF-1α (cat. no. 3716), rabbit anti-VEGF (cat. no. 2463), rabbit anti-Akt (cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (cat. no. 5012), rabbit anti-extracellular signal-regulated kinases (Erk1/2; cat. no. 4695), rabbit anti-p-Erk1/2 (cat. no. 4377) (all 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) and rabbit anti-β-actin (cat. no. sc-7210; 1:4,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Expressing, Knock-Out, Western Blot

    AuNPs reduced TGF-β1, CTGF, and VEGF production via Akt-dependent pathway. Notes: ( A ) Cell proliferation rate after AuNP treatment with different concentrations was detected by CCK-8 assay (n=6). ( B , C ) The effect of different treatments on the apoptosis of SW620 cells, no significant difference of the apoptosis rate was found after AuNP treatment with different concentrations (n=3). ( D ) The effects of AuNPs with different concentrations on TGF-β1, CTGF, and VEGF expression of SW620 cells were determined using Western blot analysis. ( E ) The effects of AuNPs on the expression of Akt and phosphorylated Akt were detected by Western blot analysis. ( F – H ) The effects of AuNPs with different concentrations on TGF-β 1, CTGF, and VEGF secretion of SW620 cells were determined using ELISA analysis (n=3; AuNPs1: 10 ng/mL; AuNPs2: 25 ng/mL; AuNPs3: 50 ng/mL; AuNPs4: 100 ng/mL). One-way ANOVA was utilized for analysis, and error bars indicate SEM ( *** P

    Journal: International Journal of Nanomedicine

    Article Title: Gold nanoparticles enhance cisplatin delivery and potentiate chemotherapy by decompressing colorectal cancer vessels

    doi: 10.2147/IJN.S176928

    Figure Lengend Snippet: AuNPs reduced TGF-β1, CTGF, and VEGF production via Akt-dependent pathway. Notes: ( A ) Cell proliferation rate after AuNP treatment with different concentrations was detected by CCK-8 assay (n=6). ( B , C ) The effect of different treatments on the apoptosis of SW620 cells, no significant difference of the apoptosis rate was found after AuNP treatment with different concentrations (n=3). ( D ) The effects of AuNPs with different concentrations on TGF-β1, CTGF, and VEGF expression of SW620 cells were determined using Western blot analysis. ( E ) The effects of AuNPs on the expression of Akt and phosphorylated Akt were detected by Western blot analysis. ( F – H ) The effects of AuNPs with different concentrations on TGF-β 1, CTGF, and VEGF secretion of SW620 cells were determined using ELISA analysis (n=3; AuNPs1: 10 ng/mL; AuNPs2: 25 ng/mL; AuNPs3: 50 ng/mL; AuNPs4: 100 ng/mL). One-way ANOVA was utilized for analysis, and error bars indicate SEM ( *** P

    Article Snippet: TGF-β1, CTGF, VEGF, and phosphorylated Akt were analyzed using a TGF-β Rabbit mAb (no 3709), Rabbit CTGF mAb (no 86641), Rabbit VEGF antibody (no 2463), Akt mAb (no 4691), and Akt rabbit phospho-Akt mAb (no 4060; Cell Signaling, Beverly, MA, USA).

    Techniques: CCK-8 Assay, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Gene expression analysis of GEnCs cultured on hydrogel substrates at designated time points. (A)  PECAM1  encoding for platelet endothelial cell adhesion molecule or CD31. (B)  CDH5  encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C)  ITGB1  encoding for integrin subunit β1. (D)  ITGB3  encoding for integrin subunit β3. (E)  KDR  encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F)  TEK  encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G)  VWF  encoding for von Willebrand factor. (H)  NOS3  encoding for nitric oxide synthase 3. (I)  PLVAP  encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression  normalized to gene expression of GEnCs cultured in tissue culture polystyrene on day 0 ( n  = 4).

    Journal: Biomaterials

    Article Title: Poly(Ethylene Glycol)-Crosslinked Gelatin Hydrogel Substrates with Conjugated Bioactive Peptides Influence Endothelial Cell Behavior

    doi: 10.1016/j.biomaterials.2019.02.001

    Figure Lengend Snippet: Gene expression analysis of GEnCs cultured on hydrogel substrates at designated time points. (A) PECAM1 encoding for platelet endothelial cell adhesion molecule or CD31. (B) CDH5 encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C) ITGB1 encoding for integrin subunit β1. (D) ITGB3 encoding for integrin subunit β3. (E) KDR encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F) TEK encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G) VWF encoding for von Willebrand factor. (H) NOS3 encoding for nitric oxide synthase 3. (I) PLVAP encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression normalized to gene expression of GEnCs cultured in tissue culture polystyrene on day 0 ( n = 4).

    Article Snippet: Primary antibodies were diluted in Sea Block as follows: mouse anti-PECAM-1 at 1:100 (Abcam, #ab187377) and rabbit anti-VEGF Receptor 2 at 1:200 (Cell Signaling Technology, #2479).

    Techniques: Expressing, Cell Culture

    Gene expression analysis and immunofluorescence staining of HUVECs cultured on hydrogel substrates at designated time points. (A)  PECAM1  encoding for platelet endothelial cell adhesion molecule or CD31. (B)  CDH5  encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C)  ITGB1  encoding for integrin subunit β1. (D)  ITGB3  encoding for integrin subunit β3. (E)  KDR  encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F)  TEK  encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G)  VWF  encoding for von Willebrand factor. (H)  NOS3  encoding for nitric oxide synthase 3. (I)  PLVAP  encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression normalized to gene expression of HUVECs cultured in tissue culture polystyrene on day 0 ( n  = 4). (J) Whole-mount immunofluorescence staining of HUVECs cultured on hydrogel substrates at 6 and 24 hrs. Merged images: PECAM-1 (green), VEGFR2 (red), and DAPI (blue).

    Journal: Biomaterials

    Article Title: Poly(Ethylene Glycol)-Crosslinked Gelatin Hydrogel Substrates with Conjugated Bioactive Peptides Influence Endothelial Cell Behavior

    doi: 10.1016/j.biomaterials.2019.02.001

    Figure Lengend Snippet: Gene expression analysis and immunofluorescence staining of HUVECs cultured on hydrogel substrates at designated time points. (A) PECAM1 encoding for platelet endothelial cell adhesion molecule or CD31. (B) CDH5 encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C) ITGB1 encoding for integrin subunit β1. (D) ITGB3 encoding for integrin subunit β3. (E) KDR encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F) TEK encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G) VWF encoding for von Willebrand factor. (H) NOS3 encoding for nitric oxide synthase 3. (I) PLVAP encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression normalized to gene expression of HUVECs cultured in tissue culture polystyrene on day 0 ( n = 4). (J) Whole-mount immunofluorescence staining of HUVECs cultured on hydrogel substrates at 6 and 24 hrs. Merged images: PECAM-1 (green), VEGFR2 (red), and DAPI (blue).

    Article Snippet: Primary antibodies were diluted in Sea Block as follows: mouse anti-PECAM-1 at 1:100 (Abcam, #ab187377) and rabbit anti-VEGF Receptor 2 at 1:200 (Cell Signaling Technology, #2479).

    Techniques: Expressing, Immunofluorescence, Staining, Cell Culture

    VEGF-A secreted by BM-MSC activate LEC. (A) Western blot analyses of VEGF-A and VEGF-C production on serum-free EBM-2 (CTR) and MSC conditioned medium (MSC CM). (B) VEGFR-2 (top) and VEGFR-3 (bottom) proteins were detected following a phosphorylated tyrosine-containing protein (pY) immunoprecipation (IP) of LEC lysates after cell stimulation with control medium (CTR) or with MSC conditioned medium (MSC CM). Cells treated with VEGF-A (10 ng/ml) or VEGF-C (400 ng/ml) were used as negative and positive controls, respectively. GAPDH western blot was performed on the flowthrough of each sample.

    Journal: PLoS ONE

    Article Title: Bone Marrow-Derived Mesenchymal Stem Cells Drive Lymphangiogenesis

    doi: 10.1371/journal.pone.0106976

    Figure Lengend Snippet: VEGF-A secreted by BM-MSC activate LEC. (A) Western blot analyses of VEGF-A and VEGF-C production on serum-free EBM-2 (CTR) and MSC conditioned medium (MSC CM). (B) VEGFR-2 (top) and VEGFR-3 (bottom) proteins were detected following a phosphorylated tyrosine-containing protein (pY) immunoprecipation (IP) of LEC lysates after cell stimulation with control medium (CTR) or with MSC conditioned medium (MSC CM). Cells treated with VEGF-A (10 ng/ml) or VEGF-C (400 ng/ml) were used as negative and positive controls, respectively. GAPDH western blot was performed on the flowthrough of each sample.

    Article Snippet: After 4 h precipitation using protein A sepharose beads (GE Healthcare, Diegem, Belgium), proteins were released from the beads by heating 5 min at 95°C in sample buffer (50 mM TrisHCl pH6.8, 4% SDS, 1% beta-mercaptoethanol, 20% glycerol, 0,05% bromophenol blue) and were subjected to VEGFR-2 and -3 Western-Blotting experiments (1/1000; rabbit monoclonal anti-VEGFR-2, Cell Signaling, San Diego, CA and 1/1000; mouse monoclonal anti-VEGFR-3, Millipore, Carrigtwohill, Ireland).

    Techniques: Western Blot, Cell Stimulation