rabbit anti vegf (Cell Signaling Technology Inc)
Name:
Rabbit Anti Mouse IgG Light Chain Specific D3V2A mAb HRP Conjugate
Description:
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase HRP via its amine groups
Catalog Number:
58802
Price:
None
Category:
Secondary Antibodies
Source:
Monoclonal antibody is produced by immunizing animals with native total mouse IgG.
Applications:
Western Blot
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Structured Review

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase HRP via its amine groups
https://www.bioz.com/result/rabbit anti vegf/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis"
Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
Journal: Experimental and Therapeutic Medicine
doi: 10.3892/etm.2018.6397

Figure Legend Snippet: The correlation analysis between the relative protein expression level of Wnt1 and that of VEGF in the aorta of the rats. The protein expression level of Wnt1 in the aorta of the rats is positively correlated with that of VEGF (P
Techniques Used: Expressing

Figure Legend Snippet: Detection of relevant protein expression levels in the aorta of the rats in each group with western blot analysis. (A) Strip figure. (B) Relative protein expression level of Wnt1. (C) Relative protein expression level of β-catenin. (D) Relative protein expression level of DKK1. (E) Relative protein expression level of VEGF. The protein expression levels of Wnt1, β-catenin, DKK1 and VEGF in the aorta of the rats in the model group are significantly higher than those of the rats in the control group (**P
Techniques Used: Expressing, Western Blot, Stripping Membranes
2) Product Images from "Hyperactivated FRS2α-mediated signaling in prostate cancer cells promotes tumor angiogenesis and predicts poor clinical outcome of patients"
Article Title: Hyperactivated FRS2α-mediated signaling in prostate cancer cells promotes tumor angiogenesis and predicts poor clinical outcome of patients
Journal: Oncogene
doi: 10.1038/onc.2015.239

Figure Legend Snippet: Depletion of FRS2 α reduces expression of Vegf-a in mouse TRAMP tumors A. Real-time RT-PCR analyses of the indicated gene expression in TRAMP tumors with or without tissue-specific ablation of Frs2α in the epithelial cells. B C . Immunostaining (B) and Western blot (C) analyses of cJUN and HIF1α expression in the TRAMP tumors. F/F, Frs2α flox ; CN, Frs2α CN ; *, P
Techniques Used: Expressing, Quantitative RT-PCR, Immunostaining, Western Blot
3) Product Images from "Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway"
Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Journal: Experimental and Therapeutic Medicine
doi: 10.3892/etm.2018.5783

Figure Legend Snippet: Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P
Techniques Used: Expressing, Knock-Out, Western Blot

Figure Legend Snippet: Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.
Techniques Used: Expressing, Inhibition, Translocation Assay

Figure Legend Snippet: Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P
Techniques Used: Expressing, Knock-Out, Western Blot
4) Product Images from "Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway"
Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Journal: Experimental and Therapeutic Medicine
doi: 10.3892/etm.2018.5783

Figure Legend Snippet: Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P
Techniques Used: Expressing, Knock-Out, Western Blot

Figure Legend Snippet: Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.
Techniques Used: Expressing, Inhibition, Translocation Assay

Figure Legend Snippet: Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P
Techniques Used: Expressing, Knock-Out, Western Blot
5) Product Images from "The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis"
Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
Journal: Experimental and Therapeutic Medicine
doi: 10.3892/etm.2018.6397

Figure Legend Snippet: The correlation analysis between the relative protein expression level of Wnt1 and that of VEGF in the aorta of the rats. The protein expression level of Wnt1 in the aorta of the rats is positively correlated with that of VEGF (P
Techniques Used: Expressing

Figure Legend Snippet: Detection of relevant protein expression levels in the aorta of the rats in each group with western blot analysis. (A) Strip figure. (B) Relative protein expression level of Wnt1. (C) Relative protein expression level of β-catenin. (D) Relative protein expression level of DKK1. (E) Relative protein expression level of VEGF. The protein expression levels of Wnt1, β-catenin, DKK1 and VEGF in the aorta of the rats in the model group are significantly higher than those of the rats in the control group (**P
Techniques Used: Expressing, Western Blot, Stripping Membranes
6) Product Images from "VEGF silencing inhibits human osteosarcoma angiogenesis and promotes cell apoptosis via PI3K/AKT signaling pathway"
Article Title: VEGF silencing inhibits human osteosarcoma angiogenesis and promotes cell apoptosis via PI3K/AKT signaling pathway
Journal: International Journal of Clinical and Experimental Medicine
doi:

Figure Legend Snippet: VEGF silencing inhibits the activation of PI3K and AKT in U2OS cells. The levels of p-PI3K, total PI3K, p-AKT and total AKT were detected with western blotting GAPDH was used as a control for normalization of the total protein loading.
Techniques Used: Activation Assay, Western Blot
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